Edy Djauhari Purwakusumah
Biopharmaca Study Centre, Institute of Research and Community Services, Bogor Institute of Agriculture

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Variasi Bahan Bioaktif dan Bioaktivitas Tiga Nomor Harapan Temulawak pada Lokasi Budidaya Berbeda Nurcholis, Waras; Purwakusumah, Edy Djauhari; Rahardjo, Mono; Darusman, Latifah K.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 40, No 2 (2012): JURNAL AGONOMI INDONESIA
Publisher : Bogor Agricultural University

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Abstract

Temulawak (Curcuma  xanthorrhizaRoxb.) belongs to the family Zingiberaceae, has been empirically used as herbal medicines. The research was aimed to evaluate three promising lines of Temulawak based on their high bioactive contents (xanthorrhizol and curcuminoid) and its in vitro bioactivity (antioxidant and toxicity), and to obtain information on agrobiophysic environmental condition which produced high bioactive compounds. The xanthorrhizol and curcuminoid contents were measured by HPLC. In vitro antioxidant and toxicity were determined by DPPH (1,1-diphenyl-2-picryl-hydrazyl) method and BSLT (Brine Shrimp Lethality Test). The result showed that promising line A produced the highest yield of bioactive and bioactivity, i.e. 0.157 and 0.056 g plant-1of xanthorrizol and curcuminoid respectively. The IC50 of antioxidant activity was 65.09 mg L-1and LC50of toxicity was 69.05 mg L-1. In this study, Cipenjo had the best temulawak performance than two other locations. According to the agrobiophysic parameters, Cipenjo environmental condition was suitable for temulawak cultivation with temperature 28-34 ºC, rainfall ± 223.97 mm year-1 and sandy clay soil. Keywords: antioxidant, curcuminoid, promising lines, temulawak, xanthorrhizol
Antioxidant, Cytotoxic Activities and Total Phenolic Content of Four Indonesian Medicinal Plants Nurcholis, Waras; Priosoeryanto, Bambang Pontjo; Purwakusumah, Edy Djauhari; Katayama, Takeshi; Suzuki, Toshisada
VALENSI Vol 2, No 4 (2012): Jurnal Valensi Volume 2//No.4//Mei 2012
Publisher : Program Studi Kimia, Fakultas Sains dan Teknologi, UIN Syarif Hidayatullah Jakarta

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Abstract

The crude ethanol extracts of four Indonesian medicinal plants namely Curcuma xanthorrhiza Roxb.,Phyllanthus niruri Linn., Andrographis paniculata Ness., and Curcuma aeruginosa Roxb. wereexamined for their antioxidant (radical scavenging) activity using 2, 2-diphenyl-2-picrylhydrazyl(DPPH) free radical and cytotoxicity using brine shrimp lethality test (BSLT). The total phenoliccontent was used the Folin-Ciocalteu method. IC50 values for DPPH radical scavenging activityranged from 14.5 to 178.5 μg/ml, with P. niruri having the lowest value and therefore the mostpotent, and C. aeruginosa having the highest value. LC50 values for BSLT ranged from 210.3 to593.2 μg/ml, with C. xanthorrhiza and A. paniculata having the lowest and highest values,respectively. The total phenolic content of the Indonesian plants ranged from 133.0 ±3.7 to863.3±54.7 mg tannic acid equivalent per 1 g extract, with C. aeruginosa and P. niruri having thelowest and highest values, respectively. A positive correlation between free radical scavengingactivity and the content of phenolic compounds was found in the four of Indonesian medicinal plants.
Pengembangan Model Kemitraan dan Pemasaran Terpadu Biofarmaka dalam Rangka Pemberdayaan Masyarakat Sekitar Hutan di Kabupaten Sukabumi, Provinsi Jawa Barat Sundawati, Leti; Purnaningsih, Ninuk; Purwakusumah, Edy Djauhari
Jurnal Ilmu Pertanian Indonesia Vol 17, No 3 (2012): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Most of the community surrounding forest areas in Sukabumi District are poor farmers. They traditionally cultivate medicinal plants. However, quality of the products is not standardized, and hence, low price. Therefore, it is needed to strengthen and empower the farmer capacity, by developing an integrated marketing model for medicinal plants and by enhancing the competitiveness of the farmer group institution so that they can build a partnership with industry. This activity involved farmers at Mekarjaya Village, Ciemas Subdistrict who are member of Srijaya Farmer Groups Association. Survey, in-depth interview, and focus group discussion were conducted to get data on the existing condition. Farmer institution strengthening was conducted through trainings for farmers on entrepreneurship, medicinal plant cultivation and processing, facilitation of farmer group institution, and establishment of demonstration plots of medicinal plant cultivation. Agribusiness networking and partnership was built through the memorandum of understanding on marketing between Sri Jaya with two biopharmaca companies. Keywords: empowerment, farmer institution, marketing, medicinal plant, partnership
Pengembangan Model Kemitraan dan Pemasaran Terpadu Biofarmaka dalam Rangka Pemberdayaan Masyarakat Sekitar Hutan di Kabupaten Sukabumi, Provinsi Jawa Barat Sundawati, Leti; Purnaningsih, Ninuk; Purwakusumah, Edy Djauhari
Jurnal Ilmu Pertanian Indonesia Vol 17, No 3 (2012): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (320.236 KB)

Abstract

Most of the community surrounding forest areas in Sukabumi District are poor farmers. They traditionally cultivate medicinal plants. However, quality of the products is not standardized, and hence, low price. Therefore, it is needed to strengthen and empower the farmer capacity, by developing an integrated marketing model for medicinal plants and by enhancing the competitiveness of the farmer group institution so that they can build a partnership with industry. This activity involved farmers at Mekarjaya Village, Ciemas Subdistrict who are member of Srijaya Farmer Groups Association. Survey, in-depth interview, and focus group discussion were conducted to get data on the existing condition. Farmer institution strengthening was conducted through trainings for farmers on entrepreneurship, medicinal plant cultivation and processing, facilitation of farmer group institution, and establishment of demonstration plots of medicinal plant cultivation. Agribusiness networking and partnership was built through the memorandum of understanding on marketing between Sri Jaya with two biopharmaca companies.
Geographical classification of Java Tea (Orthosiphon stamineus) from Java Island by FTIR Spectroscopy Combined with Canonical Variate Analysis Rafi, Mohamad; Purwakusumah, Edy Djauhari; Ridwan, Taopik; Barus, Baba; Sutandi, Atang; Darusman, Latifah Kosim
JURNAL SAINS DAN MATEMATIKA Volume 23 Issue 1 Year 2015
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

FTIR spectroscopy combined with canonical variate analysis was used for differentiation of java tea (Orthosiphon stamineus) according to their geographical origin. FTIR spectra of all java tea samples were acquired in the mid infrared region (wavenumber range 4000-400 cm-1). Preprocessing signal of FTIR spectra has been carried out prior to canonical variate analysis by standard normal variate. Combination of FTIR spectra in the region 1800-900 cm-1with canonical variate analysis has the power to differentiate java tea samples in terms of geographical origin. The developed method could be used for identification of geographical origin of java tea based on the samples used in this study.
Identifikasi dan Autentikasi Jahe Merah Menggunakan Kombinasi Spektroskopi FTIR dan Kemometrik Purwakusumah, Edy Djauhari; Rafi, Mohamad; Safitri, Utami Dyah; Nurcholis, Waras; Zaim Adzkiya, Muhammad Agung
Agritech Vol 34, No 1 (2014)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.9526

Abstract

FTIR spectroscopy combined with principal component analysis and discriminant analysis were used for identification and authentication of jahe merah from its related species namely jahe emprit and jahe gajah. FTIR spectra of all samples were recorded in the wavenumber range 4000-400 cm-1 and then subjected for preprocessing signal such as normalization and baseline correction. Combination of FTIR spectra with some chemometrics method such as principal component analysis and discriminant analysis were used to distinguish the three varieties of ginger. By using discriminant analysis, the three varieties of ginger were classified according to its variety. The developed method could be used for identification and authentication of jahe merah.ABSTRAKSpektroskopi FTIR dan kombinasinya dengan analisis komponen utama dan analisis diskriminan telah digunakan untuk identifikasi dan autentikasi jahe merah dari dua spesies yang berkerabat dengannya yaitu jahe emprit dan jahe gajah. Seluruh contoh dibuat spektrum FTIR pada kisaran bilangan gelombang 4000-400 cm-1 dan diberi perlakuan pendahuluan seperti normalisasi dan koreksi garis dasar. Kombinasi spektrum FTIR dengan beberapa metode kemometrik seperti analisis komponen utama dan analisis diskriminan digunakan untuk membedakan ketiga varietas jahe. Analisis diskriminan dapat mengelompokkan ketiga varietas jahe sesuai dengan jenis varietasnya. Metode yang dikembangkan ini dapat digunakan untuk tujuan identifikasi dan autentikasi jahe merah.
Antioxidant Status of Sprague-Dawley Female Rat with Curcuminoids Nanoparticles of Balittro Curcuma Ambarsari, Laksmi; Febrianti, Riska; Purwakusumah, Edy Djauhari
Current Biochemistry Vol 4, No 1 (2017)
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/15-37

Abstract

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Evaluasi Aktivitas Antioksidan dan Perubahan Metabolit Sekunder Mayor Temulawak (Curcuma xanthorriza) Pada Umur Rimpang Yang Berbeda Purwakusumah, Edy Djauhari; Royani, Lusi; Rafi, Mohamad
Jurnal Jamu Indonesia Vol 1 No 1 (2016): Jurnal Jamu Indonesia
Publisher : Pusat Studi Biofarmaka Tropika LPPM IPB; Tropical Biopharmaca Research Center - Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (921.368 KB) | DOI: 10.29244/jji.v1i1.3

Abstract

Penelitian ini dilakukan untuk mengevaluasi aktivitas antioksidan dan perubahan metabolit sekunder mayor temulawak (C. xanthorrhiza) pada umur rimpang yang berbeda. Aktivitas antioksidan ditentukan menggunakan tiga metode yaitu DPPH (1,1-difenil-1-pikrilhidrazil), FRAP (ferric reducing antioxidant power), dan CUPRAC (cupric ion reducing antioxidant capacity), sedangkan kadar kurkuminoid dan xantorizol ditentukan dengan metode KCKT. Hasil pengukuran aktivitas antioksidan, kadar kurkuminoid dan xantorizol menunjukkan semakin meningkat dengan bertambahnya umur rimpang temulawak. Analisis korelasi antara aktivitas antioksidan dan kadar kurkuminoid serta xantorizoldiperoleh bahwa kapasitas antioksidan yang diukur dengan metode DPPH dan FRAP mempunyai korelasi positif dengan nilai sebesar 0.757  r  0.996 dan 0.522  r  0.976 berturut-turut, sedangkan metode CUPRAC berkorelasi tinggi dengan jumlah rendemen (r = 0.986). Analisis spektrum IR pada sampel rimpang temulawak memberikan profil yang identik dengan perbedaan hanya pada nilai absorbansnya. Rimpang temulawak dengan umur 9 bulan memiliki detail spektrum IR yang lebih jelas dan nilai absorbans yang lebih tinggi dibandingkan dengan umur rimpang 7 dan 8 bulan. Evaluasi yang dilakukan dapat memberikan informasi mengenai mutu rimpang temulawak berdasarkan masa tanamnya.