Articles

Found 19 Documents
Search

isolation and Selection ofExtremophillic Cellulase Producing Bacteria Isolatedfrom Black Water Ecosystem ., Fikrinda; Anas, Iswandi; Purwadaria, Tresnawati; Santosa, Dwi Andreas
Jurnal Mikrobiologi Indonesia Vol 5, No 2 (2000): JURNAL MIKROBIOLOGI INDONESIA
Publisher : Jurnal Mikrobiologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The cellulolytic bacteria are potential organisms for biotechnological applications such as in decomposition oflignocellulose materials and enzyme technology. The black water ecosystem is a unique ecosystem because of its highdiversity of flora, fauna, and microbes. The aims of this experiment were to isolate cellulolytic bacteria from theecosystem of black water and to select their ability to grow at the extrem conditions low and high plls and hightemperature. The total number of cellulolytic bacteria isolated at 30°C with pH 4 or 7 and at 50°C with pH 4 or 7 was1.263. In further screening at higher temperatures, 30 bacteria were obtained at 60°C while at 70°C only 16 bacteriawere selected. The isolates were furthter screened at different pH based on their optimal growth temperature, andthey were able to grow at pH 3-11. Besides bacteria that grow at normal temperature 4-40°C and at p11 neutral 5.8.5there at high temperatures 60 and 70°C and low and high pils 3 and 10, 11 showing that there are high diversity inthe ecosystem of black water.
Apparent Induction of Xylanase by Bacillus pumilus PU4-2 using Pretreated Substrates WIDJAJA, SHERLY; PURWADARIA, TRESNAWATI; KETAREN, PIUS PERTUMPUN
Microbiology Indonesia Vol 2, No 1 (2008): April 2008
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Bacillus pumilus PU4-2 produces xylanase (â-1,4-D-xylan xylanohydrolase; EC 3.2.1.8) in wheat pollard with high activity. Water and NaOH-soaked pollard were used in this research to enhance the production of assayable enzyme. Enzyme activity was produced in minimal media containing 3% w/v untreated or water or NaOH-soaked pollards in 250 ml flasks incubated on shaker incubator at 30°C and 150 rpm for 36 h. The production was also compared to untreated oatmeal known as an inducer substrate. The highest xylanase activity was obtained by using untreated pollard as a sole carbon source. The enzyme activity was 157 U ml-1 with specific activity at 718 U mg-1. Xylanase production using different soaking time for water pretreated pollard also confirmed that untreated pollard was the best inducer. The production was not influenced by different water soaking times used to remove reducing sugar. Although pretreatment decreased the reducing sugar, the reduction did not enhance assayable enzyme levels. The production was best induced by the soluble oligosaccharides of untreated pollard. We conclude that B. pumilus PU4-2 was able to produce xylanase with reducing sugar content up to 660 ppm present in production medium. With this reducing sugar level, repression of enzyme production was not detected in the production medium.
The Production of Nata Colored by Monascus purpureus J1 Pigments as Functional Food PURWADARIA, TRESNAWATI; GUNAWAN, LISRINA; GUNAWAN, AGUSTIN WYDIA
Microbiology Indonesia Vol 4, No 1 (2010): April 2010
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pigments from Monascus sp. may color nata. The mold also produces monacolin K that inhibits HMG-CoA reductase affecting the reduction of blood cholesterol. The aim of this study was to produce the colored nata containing monacolin K. The mold was isolated from commercial angkak. Potato sucrose (PS) and synthetic glucose (SG) media were used to ferment nata with Monascus purpureus J1. Fermentation of nata in PS medium produced red nata, while that in SG medium produced orange nata. The color of nata was similar to the color of supernatant. The optimum red production was obtained after five days of incubation, while the orange production increased until the 14th day. The color concentrations of the supernatant of PS medium containing nata (35.4 μg mL-1) were lower than those without nata (12.4 μg mL-1). The colors of nata looked darker than the color of the supernatant. The concentration of monacolin K in the red nata and the supernatant of PS medium were 0.6 μg mL-1 and 4.6 μg mL-1 respectively, while those in the orange nata and the supernatant of  SG  medium were 3.2 μg mL-1 and 14.6  μg mL-1 respectively. Dry matter biomass in the PS medium was lower than that in the SG medium. Even though the color of nata looked relatively stable, analyses of the nata water extract  that showed a stable condition only occurred in  freezing (-20OC) and soaking in buffer solution of pH 12; boiling, water  washing, and soaking in a solution of pH 3 and 7 reduced the pigment concentration. Monacolin K concentration was not stable for every treatment, especially for water washing and freezing. Eventhough it was not stable, the boiling nata contained red pigments and monacolin K of 19.7 μg mL-1 and 0.1 μg mL-1 respectively, which can be served as functional food.
Isolation of Endophytic Bacteria from Palm Oil Fruits and Characterization of Their Lipases DJAFAR, FANDY; PURWADARIA, TRESNAWATI; SINURAT, ARNOLD PARLINDUNGAN
Microbiology Indonesia Vol 4, No 2 (2010): August 2010
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Lipases (EC 3.1.1.3)  can be produced in palm oil fruits by fruit cells or endophytic microbes. The purpose of this research is to isolate endophytic lipolytic bacteria of palm oil fruit and characterization their lipases. The bacteria were isolated and screened using medium Kouker and Jaeger containing olive oil,  minerals, yeast extract, peptone, agar, and rhodamine B as an indicator. Fifteen endophytic bacteria were isolated and identified having the microbial lipase activity. Most of them showed rod shape, positively Gram test, spore formation, and motility except one bacteria strain K which was coccus. The enzyme was produced using submerged culture in the same medium but not containing agar and rhodamine B. Based on data of enzyme activity towards p-nitrophenyl palmitate as a substrate, protein concentration, and  specific  activity, two bacteria were selected, those were BSWt2(1) and Ink 1.3 isolates. Microscopic and biochemistry analyses show that BSWt2(1) and Ink 1.3 were identified as Bacillus brevis and B. lacterosporus respectively. Crude lipase from B. brevis BSWt2(1) and B. lacterosporus Ink 1.3 showed optimum activities at pH 8.0-9.0 and 60°C, and at pH 8.5 and 60-70°C. The enzymes were stable pre-incubated at pH 7.5-9.0 and pH 7.5-8.0 respectively, and they were stable pre-incubated for 2-4 hours at 80°C and for 2-8 hours at 100°C.  Based on  stability in high temperature, lipase from both isolates were specific and might be applicable for use in waste water treatment in palm oil factories.
Isolation and Screening of Microbes for The microorganism used as probiotics must be bio-safety, could be cheaply and easily Purwadaria, Tresnawati; Kompiang, I Putu; Darma, Jinadasa; ., Supriati; Sudjatmika, Emi
Jurnal Ilmu Ternak dan Veteriner Vol 8, No 2 (2003): JUNE 2003
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.605 KB) | DOI: 10.14334/jitv.v8i2.376

Abstract

produced, and suitable with the environment of the digestive track. Isolation was carried out from commercial culture (containing mixture of Bacillus spp.), digestive tract (proventriculus, small intestine and large intestine) of local and broiler chickens, and commercial yoghurts (fermented milk). Neutral and acidic nutrient agars (NA) were used as the media at room temperature and in the aerobic or anaerobic conditions. Separate colonies were isolated, stained with Gram and spore staining and observed under the microscope. The bacteria which were Gram positive bacillus and can form spores were further identified. Eight different species of Bacillus spp.: B. laterosporus, B. coagulans, B. alvei, B. circulans, B. brevis B. bodius, B. pasteurii, and B. macroides were isolated from the commercial mixture. From the digestive tracts of local and broiler chickens, 13 bacteria and 2 yeasts were isolated, while 5 yeast were obtained from two commercial yoghurts. Those bacteria were facultative aerobic and only grew in neutral condition and not in acidic condition (pH 4.5), while the yeast were either facultative anaerobic also can grow in pH neutral and 4.5. The ability of each isolates to grow in the media containing mixture of minerals and glucose, sucrose or molasses were evaluated. Incubation was carried out in the shaker incubator at 40°C, 150 rpm for 48 hours. Dry matter of the biomass was determined as the growth parameter. All isolates of bacteria and yeast can grow in the substrate containing glucose, sucrose and molasses. Variance analyses show that there were interactions between kind of bacteria and carbon source or between kind of yeast and carbon source (P<0.05). Three isolates of bacteria that had highest production of biomass were B. coagulans on sucrose (the third for molasses), Sp. 9 on glucose, and B. apiarius on sucrose (the sixth on molasses). B. apiarius isolated from digestive tract of local chicken will be useful to be developed as probiotics. The highest production of yeast was the control Saccharomyces cerevisiae on three sugars. Between the isolates from digestive tract of local chicken, Toluraspora delbrueckii AL-15 produced 13.8x10-1mg/ml biomassa on molasses.   Key words: Poultry probiotics, bacterial and yeast isolates, glucose, sucrose, molasses
Substitutions of soybean meal with enriched palm kernel meal in laying hens diet Sinurat, Arnold P.; Purwadaria, Tresnawati; Ketaren, Pius P.; Pasaribu, Tiurma
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 3 (2014)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v19i3.1081

Abstract

A series of experiment was conducted in order to substitute soybean meal (SBM) with palm kernel cake (PKC) as a protein source in laying hens diet. First experiment was to increase its protein and amino acids content by fermentation process and followed by enzymes supplementation to improve nutrient digestibilities. Second experiment was conducted to evaluate the effectiveness of enzyme- supplemented fermented palm kernel cake (EFPKC) to replace SBM in laying hens diet. The energy (AME) of the PKC, the fermented PKC (FPKC) and the EFPKC was measured by ileal amino acids digestibility (IAAD) in broilers. The AME and the IAAD values of the EFPKC were used for diet formulation in the feeding trial. A feeding trial was performed in laying hens, aged 51 weeks for 8 weeks egg production. Five diets with different levels of substitution of SBM with EFPKC but similar nutrient contents were formulated, i.e.: 1. Control (without EFPKC), 2. 25% of SBM in control diet substituted with EFPKC, 3. 50% of SBM in control diet substituted with EFPKC, 4. 75% of SBM in control diet substituted with EFPKC and 5. 100% of SBM in control diet substituted with EFPKC. Each diet was fed to 24 hens (6 replicates of 4 birds/ replicate). Results of the experiment showed that the fermentation of PKC increased the crude protein and most of the amino acids contents except the threonine and arginine, but decreased its AME.  Supplementation of enzymes (BS4 or CE) improved the ileal amino acid digestibilities of the fermented PKC. However, only BS4 enzymes increased the AME of the EFPKC. About 25% to 50% of the SBM in the diet could be substituted with the EFPKC without any detrimental effect on the performances of laying hens. Key Words: Soybean Meal, Palm Kernel Cake, Fermentation, Enzyme, Egg Production
Isolation and Screening of Microbes for The microorganism used as probiotics must be bio-safety, could be cheaply and easily Purwadaria, Tresnawati; Kompiang, I Putu; Darma, Jinadasa; ., Supriati; Sudjatmika, Emi
Indonesian Journal of Animal and Veterinary Sciences Vol 8, No 2 (2003)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v8i2.376

Abstract

produced, and suitable with the environment of the digestive track. Isolation was carried out from commercial culture (containing mixture of Bacillus spp.), digestive tract (proventriculus, small intestine and large intestine) of local and broiler chickens, and commercial yoghurts (fermented milk). Neutral and acidic nutrient agars (NA) were used as the media at room temperature and in the aerobic or anaerobic conditions. Separate colonies were isolated, stained with Gram and spore staining and observed under the microscope. The bacteria which were Gram positive bacillus and can form spores were further identified. Eight different species of Bacillus spp.: B. laterosporus, B. coagulans, B. alvei, B. circulans, B. brevis B. bodius, B. pasteurii, and B. macroides were isolated from the commercial mixture. From the digestive tracts of local and broiler chickens, 13 bacteria and 2 yeasts were isolated, while 5 yeast were obtained from two commercial yoghurts. Those bacteria were facultative aerobic and only grew in neutral condition and not in acidic condition (pH 4.5), while the yeast were either facultative anaerobic also can grow in pH neutral and 4.5. The ability of each isolates to grow in the media containing mixture of minerals and glucose, sucrose or molasses were evaluated. Incubation was carried out in the shaker incubator at 40°C, 150 rpm for 48 hours. Dry matter of the biomass was determined as the growth parameter. All isolates of bacteria and yeast can grow in the substrate containing glucose, sucrose and molasses. Variance analyses show that there were interactions between kind of bacteria and carbon source or between kind of yeast and carbon source (P<0.05). Three isolates of bacteria that had highest production of biomass were B. coagulans on sucrose (the third for molasses), Sp. 9 on glucose, and B. apiarius on sucrose (the sixth on molasses). B. apiarius isolated from digestive tract of local chicken will be useful to be developed as probiotics. The highest production of yeast was the control Saccharomyces cerevisiae on three sugars. Between the isolates from digestive tract of local chicken, Toluraspora delbrueckii AL-15 produced 13.8x10-1mg/ml biomassa on molasses.   Key words: Poultry probiotics, bacterial and yeast isolates, glucose, sucrose, molasses
The comparison of cellulase activities from bacteria and molds isolated from termites Purwadaria, Tresnawati; Marbun, Pesta A; Sinurat, Arnold P; Ketaren, Pius P
Indonesian Journal of Animal and Veterinary Sciences Vol 8, No 4 (2003)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v8i4.393

Abstract

Screening for choosing the best bacterium and mold producing cellulase was carried out from eight xylanolytic bacteria and three cellulolytic molds isolated from termites. Each bacterium and mold was inoculated on the agar medium containing minerals and carboxymethylcellulose. The diameters of colony and clearing zones were measured after stained with Congo red. Four bacteria: Bacillus larvae XB 1-1, B. larvae XU 2-2, Bacillus PU 2-2, and B. pumilus PU 4-2 showing higher clearing zone ratio were further submerged cultured in PM medium containing 2% wheat pollard in the shaker incubator at room temperature, 150 rpm for 36 and 48 hours. The three molds: Aspergillus flavus S 13, MS 21, and Penicillium nalgiovense S 11 were also cultured in the same way as bacteria, but in Mandels medium containing 2% wheat pollard for three and five days incubation time. B. pumilus PU 4-2 and A. flavus S 13 produced the highest CMCase in each group respectively. All enzyme assays of A. flavus S 13 had higher activities than that of B. pumilus PU 4-2. The specific activity of CMCase, avicelase, FPase, and cellobiohidrolase were the highest on the enzyme of A. flavus S 13 produced from 3 days incubation time, while its β- glucosidase was the best for five days incubation. In term of activities, A. flavus S 13 had the highest ability to produce cellulase. The ability of B. pumilus PU 4-2 to produce cellulase and xylanase at the same time was an additional value.   Key words: Bacillus pumilus, Aspergillus flavus, cellulases
Substitutions of soybean meal with enriched palm kernel meal in laying hens diet Sinurat, Arnold P.; Purwadaria, Tresnawati; Ketaren, Pius P.; Pasaribu, Tiurma
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 3 (2014): SEPTEMBER 2014
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (277.333 KB) | DOI: 10.14334/jitv.v19i3.1081

Abstract

A series of experiment was conducted in order to substitute soybean meal (SBM) with palm kernel cake (PKC) as a protein source in laying hens diet. First experiment was to increase its protein and amino acids content by fermentation process and followed by enzymes supplementation to improve nutrient digestibilities. Second experiment was conducted to evaluate the effectiveness of enzyme- supplemented fermented palm kernel cake (EFPKC) to replace SBM in laying hens diet. The energy (AME) of the PKC, the fermented PKC (FPKC) and the EFPKC was measured by ileal amino acids digestibility (IAAD) in broilers. The AME and the IAAD values of the EFPKC were used for diet formulation in the feeding trial. A feeding trial was performed in laying hens, aged 51 weeks for 8 weeks egg production. Five diets with different levels of substitution of SBM with EFPKC but similar nutrient contents were formulated, i.e.: 1. Control (without EFPKC), 2. 25% of SBM in control diet substituted with EFPKC, 3. 50% of SBM in control diet substituted with EFPKC, 4. 75% of SBM in control diet substituted with EFPKC and 5. 100% of SBM in control diet substituted with EFPKC. Each diet was fed to 24 hens (6 replicates of 4 birds/ replicate). Results of the experiment showed that the fermentation of PKC increased the crude protein and most of the amino acids contents except the threonine and arginine, but decreased its AME.  Supplementation of enzymes (BS4 or CE) improved the ileal amino acid digestibilities of the fermented PKC. However, only BS4 enzymes increased the AME of the EFPKC. About 25% to 50% of the SBM in the diet could be substituted with the EFPKC without any detrimental effect on the performances of laying hens.
Effect of enzyme supplementation on nutritive values of fermented palm kernel cake used to substitute soybean meal in broiler diet Sinurat, Arnold P.; Purwadaria, Tresnawati; Purba, Maijon
Indonesian Journal of Animal and Veterinary Sciences Vol 20, No 3 (2015): SEPTEMBER 2015
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v20i3.1185

Abstract

Two experiments was designed to improve nutritional values of palm kernel cake (PKC) by biofermentation process, followed by enzyme supplementation to substitute soybean meal (SBM) in broilers diet. A factorial of 2 x 2 design was applied in the first experiment, i.e. fermentation process (non fermented PKC and fermented PKC) and enzyme supplementation (no enzyme and +BS4 enzyme). Dry matter (DM) digestibility, AME and amino acids ileal digestibility (IAAD) of the treatment ingredients were measured in broiler chickens. Seven replications were applied for the DM and AME assays and 3 replications for IAAD assay. Second experiment was designed to study the effect of SBM substitution with enzyme supplemented FPKC (EFPKC). Four diets were formulated, i.e., control diet without EFPKC, 10%, 20 and 40% SBM substituted with EFPKC. All diets were formulated to meet the nutrient requirements of broilers. Each diet was fed to broilers from 1 to 35 d. Body weight, feed consumption, FCR and mortalities were measured. Carcass yield, abdominal fat and weight of liver and gizzard were measured at the end of experiment. Results showed that fermentation of PKC increased the DM digestibility, the AME was also increased but not significant. Enzyme supplementation did not affect the DM digestibility and AME of PKC. Fermentation process significantly (P<0.05) decreased IAAD of some indispensable amino acids. However, supplementation of enzyme did not affect the IAAD of indispensable amino acids. Substitution of soybean meal with EFPKC reduced the feed intake and growth rate of broilers. Key Words: Palm Kernel Cake, Fermentation, Enzyme, Broilers, Soybean Meal