YULIA PUJIASTUTI
Department of Plant Pests and Diseases , University of Sriwijaya, Inderalaya, Ogan Ilir,30662,Indonesia

Published : 17 Documents
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Fecundity, Longevity, and Host Finding of Three Parasitoid Species of Liriomyza sativae HERLINDA, SITI; JAYA, AGUSMAN; PUJIASTUTI, YULIA; RAUF, AUNU
HAYATI Journal of Biosciences Vol 13, No 4 (2006): December 2006
Publisher : Bogor Agricultural University, Indonesia

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Liriomyza sativae is a polyphagous agromyzid leafminer and it has invaded large part of world, i.e Hemiptarsenus varicornis, Gronotoma micromorpha, and Opius dissitus. This research were conducted to investigate progeny, immature development period, longevity of female parasitoids of L. sativae, and to study female parasitoid behaviour in host finding. Host finding behaviour was observed by examining their visit frequency to the leaves that mined by leafminer larvae and healthy leaves. Results showed that H. varicornis produced more progenies (10.70 + 2.58 progenies/female), but not significantly different (P < 0.05) from progenies produced by G. micromorpha (9.90 + 3.81 progenies/female) and O. dissitus (9.60 + 3.31 progenies/female). The immature development period of G. micromorpha (25.65 + 0.38 days) was found to be longer than H. varicornis (16.14 ± 1.20 days) and O. dissitus (14.03 + 0.22 days). Significant different (P = 0.1014) of adult longevity was not found among H. varicornis (9.22 + 2.48 days), G. micromorpha (7.25 + 1.34 days), and O. dissitus (8.74 + 2.18 days). Our analyses also indicated that G. micromorpha and O. dissitus found their hosts based on the larvae mining, however, H. varicornis performed it randomly. Based on the number of progeny and longevity of adult female, all parasitoids tested may have a potential as biological control agents of leafminer, L. sativae. Key words: fecundity, longevity, behavior, Hemiptarsenus, Gronotoma, Opius, Liriomyza sativae
Quality Control of Mass Rearing of Egg Parasitoids of Yellow Rice Stem Borer Scirpophaga Incertulas Walker Wilyus, Wilyus; Nurdiansyah, Fuad; Herlinda, Siti; Irsan, Chandra; Pujiastuti, Yulia
International Journal on Advanced Science, Engineering and Information Technology Vol 3, No 1 (2013)
Publisher : International Journal on Advanced Science, Engineering and Information Technology

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The study was conducted to find out: suitability of eggs of Corcyra cephlalonica Stainton as a factitious host of S. incertulas egg parasitoids and techniques of parasitoid stock provision, serially conducted from February 2011 to December 2011. The researches were carried out in four laboratory experiments, consisting of the study of ultraviolet (UV) irradiation on C. cephalonica eggs; suitability of C. cephlalonica eggs as a factitious host of Telenomus rowani Gahan, Trichogramma japonicum Ashmead and Tetrastichus schoenobii Ferriere; storage method of C. cephalonica eggs; and storage method of parasitoids. The research design was suited to the research needs. The results showed that; The C. cephalonica eggs irradiated by the minimum of 28.48 minute 15 watt ultraviolet (UV) at a distance +15 cm between the lamp and the eggs could suppress the hatching eggs to zero (no emerged larvae). Among the three species of egg parasitoids of S. incertulas found in Jambi Province, only T. japonicum could be rearing on C. cephalonica eggs. The relationship between storage duration of C. cephalonica eggs irradiated by the 30 minutes 15 watts UV at 5oC and the emerged T. japonicum wasps was expressed by the equation of y = 31.04-1.151x, R²=0.865, P=0.000. The storage of T. japonicum pupae for six weeks at 5°C did not reduce the number of emerged T. japonicum wasps. The emerged wasps reduced significantly if the storage duration of T. japonicum pupae was increased to seven weeks or more.
Potential Use of Entomopathogenic Virus Native to Sumatra Island as Biological Control Agent of Setora nitens L. (Lepidoptera:Limacodidae), the Main Pest of Oilpalm Suparman, Suparman; Pujiastuti, Yulia; Bando, Hisanori; Asano, Shin-ichiro
International Journal on Advanced Science, Engineering and Information Technology Vol 3, No 1 (2013)
Publisher : International Journal on Advanced Science, Engineering and Information Technology

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Slug caterpillars Setora nitens, have been appearing to be more serious insect pest of oil palm as it might cause frond damages up to 90%. Many effort had been made to control the caterpillars using insecticides but the insects are still existing and causing significant damages to the palm. Microbial insecticide, especially the one developed from indigenous entomopathogenic virus, is a promising method of controlling the insect since its toxicity to non target animals and humans is extremely low. A conventional way of controlling S. nitens using crude sap of infected larvae has been applied in several oil palm plantations in Sumatra Island, but various improvements are required to make the method more effective, efficient, widely acceptable and scientifically justified. A research on the potential use of entomopathogenic virus native to Sumatra Island as biological control agent of slug caterpillar was conducted to comprehend the pathogenicity and virulence of the entomopathogenic virus and to reveal the morphological identify of its particle. The results showed that the use of virus infecting caterpillars to control the insect was quite successful in term of increasing the number of infected caterpillars and reducing the rate of population development in the field. The use of homogenized infected caterpillars to orally infect healty S. nitens caterpillars resulted in the symptoms characteristics to viral infections appeared in all treated caterpillars with various extent of symptom developments. Some caterpillars could spine cocons but failed to release adult moth. Purification of the virus particles from infected caterpillars resulted in the apperarance of white band in the sucrose gradient indicated the presence of viral RNA. Electron microscopic observation showed that the white band in the sucrose gradient contained sphericle shape of virus particles justifying that the agent infecting S. nitens caterpillars is a virus which still need further analysis for its complete identification.
Artropoda Predator Penghuni Ekosistem Persawahan Lebak dan Pasang Surut Sumatera Selatan Khodijah, Khodijah; Herlinda, Siti; Irsan, Chandra; Pujiastuti, Yulia; Thalib, Rosdah
Jurnal Lahan Suboptimal Vol 1, No 1 (2012)
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Khodijah, S. Herlinda, C. Irsan, Y. Pujiastuti, R. Thalib. 2012. Predatory Arthropods InhabitingFresh Swamp and Tidal Lowland Ecosystem in South Sumatra. J. Lahan Suboptimal 1(1):57-63 Research of predatory arthropods inhabiting fresh swamp and tidal land ecosystem in South Sumatra used survey method. The objective of this reasearch was to identify families of the predatory arthropods found in the fresh swamp and tidal land ecosystem in South Sumatra. Survey occured in January up to March 2012. The predatory arthropods were sampled using net and pitfall trapping. The predatory arthropods found were Insecta and Arachnida. Ordo of predatory insects found were Coleoptera, Dermaptera, Diptera, Hemiptera, Orthoptera, Odonata, and Hymenoptera, while predatory spiders found were ordo Araneae. Predatory arthropods inhabiting paddy canopy were almost trapped by net traps, while soil dwelling arthropods were trapped by pitfall traps. The dominant predatory insects found were families of Carabidae and staphylinidae, while dominant family of spiders was Lycosidae. Key species of the predatory artrhopods found from the fresh and tidal land ecosystem were Pheropsophus occipitalis, Paederus fuscipes, Coccinella arcuata, Pardosa pseudoannulata, Oxyopes sp., Pardosa sumatrana, Verania lineata, Broscus sp., and Chelisoches sp.
Pemanfaatan Limbah Organik dalam Pembuatan Bioinsektisda berbasis Bacillus thuringiensis sebagai Agens Pengendalian Hama Tanaman Caisim Brassica juncea Astuti, Dessy Tri; Damiri, Nurhayati; Pujiastuti, Yulia; Afriani, Siti Rakhmi
Jurnal Lahan Suboptimal : Journal of Suboptimal Lands Vol 7, No 2 (2018): JLSO
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Astuti et al, 2018. Utilization of Organic Waste in the Making of Bacillus thuringiensis-based Bioinsecticides as Agents for Control of Caisim Brassica juncea Pests. JLSO 7(2):136-143. Bacillus thuringiensis was one of the entomopathogenic bacteria that can produces toxic crystal proteine (ᴕ-endotoksin). The use of bioinsecticide as a biological agent in pest control was one component of integrated pest control (IPM). The aimed of this reseacrh was to know the population of pests in plants and the percentage of damage to plants that exist in each treatment. The research was conducted in a vegetable garden in the Banyuasin district in November 2017 until January 2018. The research method used a randomized block design with 5 treatments and 4 blocks. The treatment was Bacillus thuringiensis KJ3R5 cultured in coconut water and rice washing water  (A), Bacillus thuringiensis LC2 cultured in coconut water and tofu wastewater (B), commercial Bacillus thuringiensis (C), inorganic insecticide (sidametrin) (D), and water as control (E). The variables observed were arthropod populations on the canopy and ground surface of choy sum (Brassica rapa var. parachinensis or Brassica chinensis var. parachinensis) plants. The results showed that 4 species of pests i.e. Pyllotreta crucifera, Spodoptera litura, Plutella xylostella, and Helula sp. Treatment with Bacillus thuringiensis LC2 (B) resulted in reduction of plant damage by 61%.
Keefektivan Beauveria bassiana (Bals.) Vuill. Isolat Indigenous Pagaralam Sumatera Selatan Pada Media Beras Terhadap Larva Plutella xylostella Linn. (Lepidoptera: Yponomeutidae) PUJIASTUTI, YULIA; ., ERFANSYAH; HERLINDA, SITI
Jurnal Entomologi Indonesia Vol 3, No 1 (2006)
Publisher : Perhimpunan Entomologi Indonesia

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The objective of study was to investigate the effectiveness of Beauveria bassiana isolate cultured in half-ripe rice media against third-instar larvae of Plutella xylostella. The research used 10 isolates consisted of 4 isolates that were originnally collected from Pagaralam i.e. PD1 (isolated from P. xylostella cadaver), PD2, PD8, PD9B (from Crysodeixis chalcites cadaver), and 6 isolates from other areas as comparison, i.e. CCW3 (from Crysodeixis chalcites cadaver), BBL (from Hypothenemus hampei cadaver), CH (from Conomorpha cramerella cadaver), CPJW (from H. hampei cadavi), WC (from Nilaparvata lugens cadaver), and WSJT (from Leptocorixa acuta cadaver). The parameters that were measured were mortality of larvae, time of death and behaviour of larvae after application. Result of the test showed that B. bassiana isolated from L. acuta (WSJT isolate) caused the highest mortality i.e. 73.34 %, with the highest spore density 5.6x107 spore ml-1 (in half-rice media) and 3.0x107 spore ml-1 (GYA media). The lowest LT50 was 19.27 hours, and was obtained from the application of PD9B. After application of B. bassiana, the behaviour of larvae was slightly change from a healthy one to less in consuming of food and then die.KEY WORDS: Effectivity, Plutella xylostella, Beauveria bassiana, LT50
Uji Efikasi Bioinsektisida Jamur Entomopatogen Berformulasi Cair terhadap Plutella xylostella (L.) Di Laboratorium Nunilahwati, Haperidah; Herlinda, Siti; Irsan, Chandra; Pujiastuti, Yulia; Khodijah, .; Meidelima, Dewi
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 13, No 1 (2013): Maret, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

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Efficacy test of liquid bio-insecticide of entomopathogenic fungi in control against Plutella xylostella in the laboratory.  The insect pest P. xylostella could reduce crop production of Brassicaceae. The aim of research was to test the efficacy liquid bio insecticide with active ingredient of Beauveria bassiana and Metarhizium anisopliae fungi to control P. xylostella. Bio-insecticide was applied by spraying  on mustard leaves infested with 50 individuals of third instar larvae of P. xylostella and a density of 1x106 conidia ml-1. Larval mortality was observed every 2 hours and LT50 of larvae was calculated. The study showed that the highest percentage of mortality found in Mt ES and Mt ES (cf) isolates was 99.6%, the lowest mortality at Mt NES isolate was 96.80%. LT50 and LT95 values   Bb ES were the lowest i.e. 2.04 days and 2.95 days. The highest LT50 and LT95 of Mt NES isolate were 2.24 days and 3.32 days. The liquid bio-insecticide of entomopathogenic fungus B. bassiana and M. anisopliae were effective to control the larvae of P. xylostella.
EKSPLORASI, ISOLASI DAN SELEKSI JAMUR ENTOMOPATOGEN PLUTELLA XYLOSTELLA (LEPIDOPTERA: YPONOMEUTIDAE) PADA PERTANAMAN CAISIN (BRASSICA CHINENSIS) DI SUMATERA SELATAN Nunilahwati, Haperidah; Herlinda, Siti; Irsan, Chandra; Pujiastuti, Yulia
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 12, No 1 (2012): Maret, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

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Plutella xylostella is the most destructive insect pests of the brassicae family. The research objective was to explore, isolate and select entomopathogenic fungi as biological agents for control of P. xylostella. This study used 20 fungal isolates originating from soil and infected insects around the farmers’ field in lowland and highland of South Sumatra. The fungal isolates were tested to third instar larvae of P.xylostella. The suspension of entomopathogenic fungus was topical inoculated with a density of 1x106 conidia ml-1 on the test insect and five replicates. The result showed that the highest (83%) and the lowest (41%) mortality of the larvae P.xylostella was induced by fungal BPluS and BNIPTr, respectively. Moreover, the shortest (2.1 days) and the highest (4.3days) lethal times of the infected host were induced by fungal BPluS and BNIPTr, respectively.
POTENSI PARASITOID TELUR PENGGEREK BATANG PADI KUNING Scirpophaga incertulas Walker PADA BEBERAPA TIPOLOGI LAHAN DI PROVINSI JAMBI Wilyus, .; Nurdiansyah, Fuad; Herlinda, Siti; Irsan, Chandra; Pujiastuti, Yulia
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 12, No 1 (2012): Maret, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

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The research was conducted to analyze the diversity, dispersal and domination of the egg parasitoid species of the yellow rice stem borer Scirpophaga incertulas Walker on several land typologies in Jambi Province.  The study was conducted by survey method, from December 2010 until June 2011.  Samples of egg parasitoids of S. incertulas were collected by baiting parasitoids with eggs of S. incertulas.  The results of the research showed that there were three spesies of the egg parasitoids of S. incertulas found in Jambi Province, the most dominant was Telenomus rowani Gahan, followed by Trichogramma japonicum Ashmead and Tetrastichus schoenobii Ferriere. T. rowani and T. japonicum were found in all wetland ecosystems in Jambi Province (tidal swamp, swampy area, rainfed lowland, lowland technical irrigation, and upland technical irrigation), while T. schoenobii was found only in tidal swamp, swampy area, and lowland technical irrigation.  The highest species diversity of egg parasitoid of S. incertulas was found on tidal swamp (Shannon index 1.047), followed by swampy area, lowland technical irrigation, rainfed and upland technical irrigation area.  The average of proportion of egg masses parasitized by T. rowani, T. japonicum and T. Schoenobii were 22.58, 6.18 and 2.68% respectively. The average of individual eggs parasitized by T. rowani, T. schoenobii, and T. japnicum were 8.41, 1.67 and 1.47%   respectively.
Pemanfaatan Tumbuhan Liar Berbunga untuk Konservasi Musuh Alami Serangga di Ekosistem Kelapa Sawit di Lahan Sub-Optimal Sumatera Selatan Prabawati, Ghani; Herlinda, Siti; Pujiastuti, Yulia; karenina, Tili
Jurnal Lahan Suboptimal : Journal of Suboptimal Lands Vol 6, No 1 (2017): JLSO
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Prabawati et al, 2017. The Role of Wild Flowering Plants in Conserving Insect Natural Enemies in Lowland Oil-Palm Ecosystem of South Sumatera. JLSO 6(1):78-86.Wild flowering plants can be used in increasing the potential of insect natural enemies on oil palm plantations. This paper inventoried the diversity of natural enemies that interacted with wild flowering plants in the oil palm ecosystem. Wild flowering plants are used as feed sources and living places  which provide alternate hosts for natural enemies, namely predators and parasitoids. Some families of flowering plants associated with  parasitoids in oil palm ecosystem include Euphorbiaceae, Leguminosae, Fabaceae, Acanthaceae, Asteraceae, Polygonaceae, Rubiaceae, Capparidaceae, Lamiaceae, Turneraceae. Some plant species associated with predators in oil palm plantations include Antigonon leptosus, Elephantopus tomentosus, Nephrolepis biserata.