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Chitinase Activity in Healthy and Sclerotium rolfsii Infected Peanut PUDJIHARTATI, ENDANG; SISWANTO, SISWANTO; ILYAS, SATRIYAS; SUDARSONO, SUDARSONO
HAYATI Journal of Biosciences Vol 13, No 2 (2006): June 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.13.2.73

Abstract

The objectives of this experiment were to analyze the endo- or exo-chitinase activities of healthy and Sclerotium rolfsii infected peanuts. The experiment analyzed 24 different peanut genotypes. Results of the experiment showed chromogenic dimer was the most suitable substrate for analysing chitinase activities. Both endo- and exo-chitinases activities were detected in leaf, stem, and crown tissues. Increased in chitinase activities were detected in S. rolfsii infected peanut tissues than in healthy plant. Regression analysis showed negative slope between disease intensity and chitinase activity in S. rolfsii infected peanut tissue (R2= 0.45). Key words: Disease response, stem rot disease, endo- and exo-chitinase
Oxidative Burst, Peroxidase Activity, and Lignin Content of Sclerotium rolfsii Infected Peanut Tissue PUDJIHARTATI, ENDANG; ILYAS, SATRIYAS; SUDARSONO, SUDARSONO
HAYATI Journal of Biosciences Vol 13, No 4 (2006): December 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.13.4.166

Abstract

The objectives of this experiment were to analyse physiological responses, such as oxidative burst reaction, peroxidase activity, and lignin content of healthy and S. rolfsii-infected peanut tissues. Differences in physiological responses among 24 peanut genotypes were determined, the disease severity was calculated and used to group resistance of tested genotypes. The regressions among observed peroxidase activity, lignin content and disease severity were used to determine the possible mechanisms of S. rolfsii resistance in peanut. Peanut seeds were grown in polybag and the growing plants were inoculated at the crown, stem, and leaf tissues. Results of the experiment indicated that infection of S. rolfsii in peanut did not induce oxidative burst. However, infection of the pathogen resulted in increased peroxidase activity and lignin content in the infected tissues. Regression analysis between peroxidase activity and disease severity showed negative slopes, indicating the more resistance the genotype, the more peroxidase activity in the tissue. Regression analysis between lignin content and disease severity was not significant. Key words: hypersensitive response (HR), resistance mechanisms, Sclerotium stem rot, disease response, Arachis hypogaea
KARAKTER AGRONOMI GENOTIPE GANDUM (Triticum Aestivum L.) PADA LAHAN TROPIS DATARAN RENDAH DI INDONESIA Kurnia, Theresa Dwi; Widyawati, Nugraheni; Murdono, Djoko; Pudjihartati, Endang
Agric Vol 28 No 1 (2016)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2016.v28.i1.p95-104

Abstract

ABSTRACTDevelopment of tropical wheat that suitable to low altitude is important in Indonesia. Aims of the research was to determine agronomic traits and select of wheat genotypes adaptive to tropical lowland. Three years study was conducted by planting ten wheat genotypes in Tlogoweru village, Guntur Subdistrict, Demak, Central Java at the altitude + 20 meters above sea level, from 2013 to 2015. From ten wheat genotypes planted in 2013, five wheat genotypes were considered adaptive, i.e., ALTAR, BASRIBEY, LAJ3302, OASIS and SELAYAR. In 2014 four genotypes were selected, they are ALTAR, BASRIBEY, LAJ3302 and OASIS. Finnaly, in 2015 genotype ALTAR was prominent candidate for the lowland tropical wheat varieties in study site. Among genotypes ALTAR had highest plant height, seed weight per panicle, number of seeds per panicle, number of productive tillers and yield estimates.
ISOLASI, IDENTIFIKASI DAN ANTAGONISME IN VITRO ISOLAT TRICHODERMA SPP. ASAL KEBUN KARET BLIMBING, PEKALONGAN, JAWA TENGAH Berlian, Intan; Anarqi, Sindu; Pudjihartati, Endang
Jurnal Penelitian Karet JPK : Volume 34, Nomor 2, Tahun 2016
Publisher : Pusat Penelitian Karet - PT. Riset Perkebunan Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/ppk.jpk.v34i2.231

Abstract

Rigidoporus microporus merupakan patogen penyebab penyakit jamur akar putih (JAP) yang sangat merugikan perkebunan karet di Kebun Blimbing, Pekalongan, Jawa Tengah. Tindakan pengendalian secara preventif, kuratif dengan cara kultur teknis dan kimiawi sudah dilakukan namun belum dapat mengendalikannya. Penelitian ini bertujuan untuk mendapatkan isolat lokal perkebunan karet di Kebun Blimbing yang bersifat antagonis terhadap JAP. Penelitian terdiri dari: (1) isolasi, (2) identifikasi, dan (3) uji antagonisme dengan metode dual culture. Pengujian menggunakan rancangan acak lengkap. Penelitian ini berhasil mendapatkan sebelas isolat Trichoderma spp. dari rhizosfer tanaman karet di Kebun Blimbing. Sebelas isolat tersebut yaitu Trichoderma spp. dari Blok Sikaum TBM 1 (P1), Blok Sikaum TBM 2 (P2), Blok Sikaum TM 1 (P3), Blok Sikaum TM 2 (P4), Blok Sari TM 1 (P5), Blok Sari TM 2 (P6), Blok Sitirejo TBM 1 (P7), Blok Sitirejo TBM 2 (P8), Blok Semurup TM 1 (P9), Blok Semurup TM 2 (P10), Blok Semurup TM 3 (P11). Semua isolat Trichoderma spp. tersebut bersifat antagonis terhadap pertumbuhan R. microporus. Persentase daya hambat Trichoderma spp. terhadap R. microporus berkisar antara 36,56% sampai 69,66%. Penghambatan paling tinggi (69,66%) diamati pada isolat P9 dan terendah (36,56%) pada isolat P3.
REDESAIN SISTEM SIRKULASI, PARKIR DAN RAMBU-RAMBU LANSKAP KAMPUS 1 UNIVERSITAS KRISTEN SATYA WACANA Nugroho, Erik Kado; Pudjihartati, Endang; Simanjuntak, Bistok Hasioholan
Agric Vol 26 No 1 (2014)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2014.v26.i1.p61-74

Abstract

Circulation, signage, and parking are important parts of planning and designing campus openspace landscape. This study aimed to redesign the circulation system, signage, and parking of the landscape of campus 1 Satya Wacana Christian University in order to resolve the prevailing problems. Analysis and synthesis (redesign) using the method of “Problem Solving” was applied by following these steps (1) inventories (primary and secondary data), (2) analysis, (3) synthesis, (4) planning, and (5) design. The results showed that the problem of circulation conflict between pedestrians and vehicle users will be overcame by adding pedestrian pathways which are tailored to user population. The lack of signages indicated that user’s navigation system was weak thus the campus open-space landscape required signpost and map. The illegal parking of four-wheeled vehicles caused disturbances in several spots of the open-space. This can be addressed by diversion of vehicles parked in a location that is available and assertion of parking or parking-ban signs. The material used in the process of redesigning the pedestrian pathways and signages applies design principles.
Chitinase Activity in Healthy and Sclerotium rolfsii Infected Peanut PUDJIHARTATI, ENDANG; SISWANTO, SISWANTO; ILYAS, SATRIYAS; SUDARSONO, SUDARSONO
HAYATI Journal of Biosciences Vol 13, No 2 (2006): June 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.13.2.73

Abstract

The objectives of this experiment were to analyze the endo- or exo-chitinase activities of healthy and Sclerotium rolfsii infected peanuts. The experiment analyzed 24 different peanut genotypes. Results of the experiment showed chromogenic dimer was the most suitable substrate for analysing chitinase activities. Both endo- and exo-chitinases activities were detected in leaf, stem, and crown tissues. Increased in chitinase activities were detected in S. rolfsii infected peanut tissues than in healthy plant. Regression analysis showed negative slope between disease intensity and chitinase activity in S. rolfsii infected peanut tissue (R2= 0.45). Key words: Disease response, stem rot disease, endo- and exo-chitinase
Oxidative Burst, Peroxidase Activity, and Lignin Content of Sclerotium rolfsii Infected Peanut Tissue PUDJIHARTATI, ENDANG; ILYAS, SATRIYAS; SUDARSONO, SUDARSONO
HAYATI Journal of Biosciences Vol 13, No 4 (2006): December 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.13.4.166

Abstract

The objectives of this experiment were to analyse physiological responses, such as oxidative burst reaction, peroxidase activity, and lignin content of healthy and S. rolfsii-infected peanut tissues. Differences in physiological responses among 24 peanut genotypes were determined, the disease severity was calculated and used to group resistance of tested genotypes. The regressions among observed peroxidase activity, lignin content and disease severity were used to determine the possible mechanisms of S. rolfsii resistance in peanut. Peanut seeds were grown in polybag and the growing plants were inoculated at the crown, stem, and leaf tissues. Results of the experiment indicated that infection of S. rolfsii in peanut did not induce oxidative burst. However, infection of the pathogen resulted in increased peroxidase activity and lignin content in the infected tissues. Regression analysis between peroxidase activity and disease severity showed negative slopes, indicating the more resistance the genotype, the more peroxidase activity in the tissue. Regression analysis between lignin content and disease severity was not significant. Key words: hypersensitive response (HR), resistance mechanisms, Sclerotium stem rot, disease response, Arachis hypogaea
The Effect of Phosphorus and Potassium on the Growth and Quality of Viola (Viola cornuta L.) Seed Production Saputra, Alfa Sada; Suprihati, Suprihati; Pudjihartati, Endang
Caraka Tani: Journal of Sustainable Agriculture Vol 35, No 1 (2020): Caraka Tani in Press April 2020
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v35i1.33618

Abstract

Viola cornuta L. is an ornamental plant that is primarily propagated by high-quality seeds that have high demand in subtropical countries. The use of fertilizers according to recommendations results in the growth of parent plants below the standard. Furthermore, seed production is generated below 2 g per plant and the seed germination capacity is below 80%. In addition, phosphorus (P) and potassium (K) nutrients can increase the growth and seed production of the plants, including ornamental plants. The study aimed to determine the influence of P and K nutrients on growth and seed production of V. cornuta L. Research was carried out in November 2018 - January 2019 at PT. Selektani Horticulture, Ngablak, Magelang. There were seven treatments with four replications. Experiments were arranged according to the Randomized Complete Block Design (RCBD). Plants were grown in 5l volume-polybags. Fertilization was done by watering the planting media. Analysis of variance was performed at 95% accuracy. The study shows that P and K nutrients have a highly significant effect on height, number of flowers, number of seed per capsule, seed production and germination energy and significantly affected the number of leaves. The addition of both P and K fertilizers in the vegetative and P in the generative stage (P6) can result in the productivity of 3.28 g plant-1 and produce germination energy ?90%. It is recommended for increasing growth and seed production.