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KARAKTERISTIK ISOLAT Rhizoctonia sp. PATOGENIK DAN Rhizoctonia MIKORIZA PADA TANAMAN ANGGREK TANAH (Spathoglottis plicata) Soelistijono, Soelistijono; Sumardiyono, Christanti; Priyatmojo, Achmadi; Semiarti, Endang
Agrineḉa Vol 12, No 1 (2012): jurnal AGRINEÇA
Publisher : Agrineḉa

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Abstract

Mycorrhizal Rhizoctonia is a fungus that capable to associate with terrestrial orchids. Apart from being mycorrhizal, there are isolates of Rhizoctonia sp. that are pathogenic and caused root rot disease on Spathoglottis plicata. This study aimed to know the differences between pathogenic Rhizoctonia sp. and mycorrhizal Rhizoctonia in morphology and molecular structure using RAPD technique. The results showed that colony colour,  cell lenght and nucleus number a several isolates of pathogenic Rhizoctonia sp. and of mycorrhizal Rhizoctonia on S. plicata had no differences, but had differences on cell thickness and isolate grouping based on hyphal anastomosis test. RAPD molecular technique showed that each isolate of pathogenic Rhizoctonia sp. and mycorrhizal Rhizoctonia had differences on DNA structure.
KARAKTERISASI ISOLAT RHIZOCTONIA MIKORIZA PADA ANGGREK PHALAENOPSIS AMABILIS Soelistijono, Soelistijono; UTAMI, DWI SISILO; Priyatmojo, Achmadi
Agrineḉa Vol 12, No 2 (2012): AGRINEÇA
Publisher : Agrineḉa

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Abstract

Mycorrhizal Rhizoctonia is a fungus that capable to associate with Phalaenopsis amabilis orchid. This study was aimed to characteristic mycorrhizal Rhizoctonia that associated with P. amabilis orchid in morphology. The results showed that colony colour, cell lenght and nucleus number a several isolates of mycorrhizal Rhizoctonia on P. amabilis had no differences, but had differences on cell thickness and isolate grouping based on hyphal anastomosis test
Characterization and Pathogenicity of Fusarium oxysporum as the Causal Agent of Fusarium Wilt in Chili (Capsicum annuum L.) FERNIAH, REJEKI SITI; DARYONO, BUDI SETIADI; KASIAMDARI, RINA SRI; PRIYATMOJO, ACHMADI
Microbiology Indonesia Vol 8, No 3 (2014): September 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.8.3.5

Abstract

Fusarium wilt is a serious disease attacking chili plants in Central Java which cause lost of chili productivity. Fusarium wilt is caused by pathogenic fungi Fusarium oxysporum, which is host specific. The objectives of this research were to characterize the pathogenic F. oxysporum as the causal agent of fusarium wilt in chili plants and to observe the virulence of the pathogen. Fungal pathogen was isolated from Tawangmangu as an endemic area of fusarium wilt in Central Java. The fungi was characterized morphologically and identified molecularly by its internal transcribed spacer regions (ITS regions). Pathogenicity test was done to observe the virulence of the pathogen. One pathogenic strain was isolated from Tawangmangu, Karanganyar and was identified  morphologically and molecularly as F. oxysporum.  
Identifikasi Cendawan Penyebab Penyakit Pascapanen pada Beberapa Buah di Yogyakarta Widiastuti, Ani; Ningtyas, Ovianne Hapsari; Priyatmojo, Achmadi
Jurnal Fitopatologi Indonesia Vol 11, No 3 (2015)
Publisher : Jurnal Fitopatologi Indonesia

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Abstract

In Indonesia, high yield losses due to post-harvest diseases are often difficult to measure because research focusing on such matter are still limited. This study aimed to determine the genera of fungi that cause rot on postharvest fruit, which can be used as a basis to determine the important pathogens in the current post-harvest commodities and for further disease management. The method used is sample collection, single spore isolation, microscopic observation and identification of fungal genera. Colletotrichum sp. was sucessfuly isolated from antrachnose of papaya. Pestalotia sp. was found in the fruit rot of avocado and star fruit. Lasiodiplodia sp. was found in mango, while Pestalotia sp. and Lasiodiplodia sp. was found in both sapodilla and banana. Alternaria sp. was found in the fruit rot of pears and apples. Aspergillus sp. was found in grapes, and Fusarium sp. was isolated from pineapple fruit rot.
Peranan Faktor Cuaca terhadap Infeksi dan Perkembangan Penyakit Bercak Ungu pada Bawang Merah Hadisutrisno, Bambang; Sudarmadi, Sudarmadi; Subandiyah, Siti; Priyatmojo, Achmadi
Jurnal Perlindungan Tanaman Indonesia Vol 1, No 1 (1995)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.9351

Abstract

Purple blotch disease caused by Alternaria porri (Ell.) Cif. is one of the most important diseases on shallots. The farmers have been using fungicides as a reliable control method, however, the chemical control method has to be considered for the negative effect on the environment and the danger to the other species and causes the resistance to a certain pesticide. Therefore, it is important to develop other method that is safe, effective, and efficient, like the use of climatic data to decide the control strategy.The result of the study on ecology of purple blotch disease carried out at the experimental station in Kramat-Tegal, Kledung-Temanggung, and KP4 Kalitirto in dry and rainy season (1991/1992) suggested that climatic factors played on important role on the daily conidium dissemination of Alternaria porri and on the intensity of purple blotch disease. Daily conidium dissemination which was one of the important component in the epidemy of purple blotch disease was influenced by the temperature, relative humidity, and the wind velocity. Conidium dissemination was occurred both during the day and night and at 10.00 am-14.00 pm when the air temperature and wind velocity were high with low relative humidity, conidium dissemination was maximum. On the other hand, at 22.00 pm – 02.00 am there was minimum conidium dissemination.The result on the effects of leaf wetness period on disease intensity suggested that high humidity was not the only factor caused disease infection but it has to be supported by the presence of thin film water on the leaf surface at least for 4 hours since the attachment of the conidia on the leaves.Key words: purple blotch, climatic factor, Alternaria porri, shallot
Tipe Mating pada Empat Isolat Thanatephorus Cucumeris Anamorf: Rhizoctonia solani) Anastomosis Group (AG) 1-IC Priyatmojo, Achmadi
Jurnal Perlindungan Tanaman Indonesia Vol 12, No 2 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12077

Abstract

Four parent isolates (189, Rh28, BW3 and F-1) of Thanatephorus cucumeris (anamorph: Rhizoctonia solani) AG 1-IC were induced to produce basidiospores using soil-over culture method. All of four parent isolates could produce basidiospores. Colonies obtained from single basidiospore isolate of each parent isolate were paired on charcoal potato dextrose agar. Single basidiospore isolate having different mating type produced tuft at area of the junction of paired colonies. On the based of tuft formation, single basidiospore isolates of each parent isolate could be divided into two different mating types, therefore it is concluded that each of 189, Rh28, BW3 and F-1 isolate of T. cucumeris AG I-IC has bipolar mating type.
Ekstraksi DNA Phytophthora palmivora Isolat Kelapa Priyatmojo, Achmadi; Subandiyah, Siti
Jurnal Perlindungan Tanaman Indonesia Vol 2, No 1 (1996)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.9358

Abstract

Coconut isolate of Phytophthora palmivora DNA was extracted from sporangium and mycelium using SDS and Proteinase-K method. Sporangium and mycelium were harvested from liquid and solid culture of Potato Dextrose medium.The result of the study showed that the DNA extracted from sporangium and mycelium of 8 days solid culture was seen as distinct band. On the other hand, the dNA from sporangium and mycelium of 24 days old liquid culture was fragmented with smear band appearance.Key words: DNA, Phytophthora palmivora
KARAKTERISTIK ISOLAT Rhizoctonia sp. PATOGENIK DAN Rhizoctonia MIKORIZA PADA TANAMAN ANGGREK TANAH (Spathoglottis plicata) Soelistijono, S.; Sumardiyono, Christanti; Priyatmojo, Achmadi; Semiarti, Endang
AGRINECA Vol 12, No 1 (2012): JURNAL AGRINECA
Publisher : AGRINECA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Mycorrhizal Rhizoctonia is a fungus that capable to associate with terrestrial orchids. Apart from being mycorrhizal, there are isolates of Rhizoctonia sp. that are pathogenic and caused root rot disease on Spathoglottis plicata. This study aimed to know the differences between pathogenic Rhizoctonia sp. and mycorrhizal Rhizoctonia in morphology and molecular structure using RAPD technique. The results showed that colony colour,  cell lenght and nucleus number a several isolates of pathogenic Rhizoctonia sp. and of mycorrhizal Rhizoctonia on S. plicata had no differences, but had differences on cell thickness and isolate grouping based on hyphal anastomosis test. RAPD molecular technique showed that each isolate of pathogenic Rhizoctonia sp. and mycorrhizal Rhizoctonia had differences on DNA structure.
Masa Inkubasi Gejala Penyakit Hawar Daun Tanaman Kentang yang Diinduksi Ketahanannya oleh Jamur Antagonis Trichoderma viride Purwantisari, Susiana; Priyatmojo, Achmadi; Sancayaningsih, Retno Peni; Kasiamdari, Rina Sri
Bioma Vol. 18, No.1, Tahun 2016
Publisher : Bioma

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.14710/bioma, 18, 2, 41-47

Abstract

Late blight disease caused by Phytophthora infestans, is probably the single most important disease of potatoes worldwide. Infected plants were quickly killed and were difficult for replanting, causing significant losses for the growers. Various control methods were examined including the use of biocontrol agents of Trichoderma spp.  The biocontrol potential of Trichoderma viride against potato late blight pathogen, Phytophthora infestans, were studied  under greenhouse conditions. The research objective was to determine the ability of biocontrol agents Trichoderma viride to delay late blight disease incidence on potato plants in the field. The in vivo experiment was carried out at potato land area located at BALITSA in Cikole Sub District, Lembang District and Bandung Regency. Completely Randomized Design was used with six treatment and five replicates. The treatment tested consist of control (without pathogen fungal and antagonists fungus application too), control (with pathogen fungal application and without antagonist fungal application), chemical fungicide application, antagonist fungal application 2 weeks before planting, application 1 week after planting and both application 2 weeks before and 1 week after planting. Result of the research showed that application of antagonists fungal could delay disease intensity until 14 days. These antagonist could be used as biological agents initials to control leaf blight disease. Key words: Biological control, Potato late blight, Phytophthora infestans, Trichoderma viride
UJI PENGGABUNGAN PGPF DAN Pseudomonas putida STRAIN PF-20 DALAM PENGENDALIAN HAYATI PENYAKIT BUSUK LUNAK LIDAH BUAYA DI TANAH GAMBUT Supriyanto, .; Priyatmojo, Achmadi; Arwiyanto, Triwidodo
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 11, No 1 (2011): Maret, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (88.657 KB) | DOI: 10.23960/j.hptt.11111-21

Abstract

Aloe (Aloe vera L. Webb) planted in West Borneo peat land is well known to have the best product quality in Indonesia. Bacterial soft rot is one of the constraints on aloe cultivation on peat land.  Many methods have not given significant result for controlling this disease.  The research objectives were to study the application of PGPF and its combination with Pseudomonas putida Pf-20 for controlling aloe bacterial soft rot on peat land.  In vitro test showed that two isolates of PGPF tested had different respons when combining with P. putida Pf-20.  The bacterial P. putida Pf-20 inhibit the growth of PGPF SNTH003 (Aspergillus sp.) as 20.14% on King’s B and 7.48% on PDA, whereas SNTH001 (Penicillium sp.) as 62.4% on King’s B dan 34.39% on PDA.  Glass house trial showed that root dipping in P. putida Pf-20 suspension could not promote the growth of aloe, but could reduce the disease intensity.  The single application of SNTH001 and SNTH003 isolates were able to increase the growth of aloe compare with its combination each of PGPF isolates with P. putida Pf-20.  Single application of SNTH003, SNTH001 and P. putida Pf-20 was able to reduce the disease intensity of bacterial soft rot, but the capability decreased when each of PGPF combined with P. putida Pf-20.  However combination between SNTH003 and P. putida Pf-20 increased that disease intensity. The lowest disease intensty (25%) obtained by using  SNTH001 isolate.