R. Priyanto
Faculty of Animal Science, Bogor Agricultural University, Jl. Agatis, Darmaga Campus, Bogor 16680

Published : 38 Documents
Articles

Found 38 Documents
Search

Hubungan Keragaman Gen Leptin dengan Kualitas Fisik Daging Sapi Lokal Di Ciamis (Relationship between Leptin Gene Diversity with Physical Quality of Local Beef In Ciamis) Hilmia, Nena; Noor, R.R; Sumantri, C.; Priyanto, R.; E, Gurnadi
Jurnal Ilmu Ternak Universitas Padjadjaran Vol 15, No 2 (2015)
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Original Source | Check in Google Scholar | Full PDF (483.972 KB)

Abstract

Leptin  terlibat  dalam  berbagai  proses  fisiologispembentukan  lemak.  Keragaman  gen Leptin  karena  adanya  SNP  Arg25Cys  pada  exon  2,  mempunyai  hubungan  dengan  deposisi lemak, yang dapat mempengaruhi  kualitas daging. Penelitian  ini bertujuan  untuk mengetahui keragaman gen leptin  dan hubungannya dengan kualitas fisik daging sapi lokal di Ciamis Jawa Barat.  Penelitian  ini  menggunakan  14  sampel  daging  bagian  udamaru  yang  diperoleh  dari peternakan  rakyat.Sampel  DNA diisolasi  dari daging.  Amplifikasi  DNA menggunakan  PCR, dan   penentuan   genotipe   dianalisis   dari   hasil   sequencing   produk   PCR.   Hasil   penelitian menunjukkan Gen Leptin pada sapi lokal Ciamis bersifat polimorfik, terdapat tiga alel, yaitu C, T (memiliki mutasi Arg25Cys) dan H (memiliki mutasi Arg25His) serta terdapat tiga genotipe CC,  CT  dan  CH.  Perbedaan  genotipe  gen  Leptin  tidak  berpengaruh  terhadap  kualitas  fisik daging sapi lokal di Ciamis.Kata kunci: Kualitas daging, Leptin
PENGARUH PEMBERIAN AMPAS SAGU, LIMBAH UDANG DAN KOMBINASINYA TERHADAP KADAR KOLESTEROL DAN PERTUMBUHAN TIKUS PUTIH Ralahalu, T.N.; Kartiarso, -; Parakkasi, A.; Wiryawan, K.G.; Priyanto, R.
JURNAL ILMU DAN TEKNOLOGI PETERNAKAN Vol 1, No 3 (2011)
Publisher : Fakultas Peternakan Universitas Hasanuddin

Show Abstract | Original Source | Check in Google Scholar | Full PDF (19.032 KB)

Abstract

Cholesterol is very beneficial for the body but it needs attention when its level is increasing in the blood. The objective of the study was to determine cholesterol level and growth rate of white rat fed on ration containing sago waste, shrimp waste or their combinations. Fourty males Spraque dowley rats, aged two months with average body weight 195.62 g, were randomly assigned to one of ten experimental treatments according to completely randomised design with four replication for each treatment combination. The treatments were  R0 (control diet), ASA 20 LU 0 (20% sago waste  and 0% shrimp waste),  ASA 0 LU 10 (0% sago waste and 10% shrimp waste), ASA 17.5 LU 2.5 (17.5% sago waste and 2.5% shrimp waste), ASA 15 LU 5 % (15% sago waste  and 5% shrimp waste), ASA 12.5 LU 7.5  (12.5% sago waste  and 7.5% shrimp waste),  ASA 10 LU 10 (10% sago waste  and 10% shrimp waste), ASA 7.5 LU 12.5  (7.5% sago waste and 12.5% shrimp waste), ASA 5 LU 15  (5% sago waste and 15% shrimp waste %), ASA 2.5 LU 17.5 (2.5% sago waste  and 17.5% shrimp waste).Variables measured included feed intake, live weight gain, plasma cholesterol, HDL, LDL, triglyceride and meat cholesterol. The results showed that treatments had no (P>0.05) effects on feed intake, live weight gain, feed efficiency, plasma HDL and LDL as well as meat cholesterol. However significant differences (P<0.01) were found on plasma cholesterol and plasma triglyceride due to treatment. The lowest levels of plasma cholesterol and plasma triglyceride were observed for treatment ASA 15 LU which were 45.95 mg/dl and 43.81 mg/dl,crespectively. It can be concluded that levels of sago waste, shrimp waste and the combinations in the ration did not affect feed intake, weight gain, feed efficiency, plasma HDL, plasma LDL and meat cholesterol but it affected plasma triglycerides and plasma cholesterol of rat. Key words: Sago waste, Shrimp waste, Rat
Daya Dukung dan Eisiensi Produksi Sapi Madura dengan Pemanfaatan Limbah Kacang Kedalai Rab, S. A.; Priyanto, R.; Fuah, A. M.; Wiryawan, I. K. G.
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol 4, No 3 (2016): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Bogor Agricultural University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (310.16 KB)

Abstract

 The aim of this study was to evaluate productivity of beef cattle through the utilization soybean by-product.Twelve Madura cattle with and average initial liveweight of 175.64 ± 16.41 kg and aged between I1-I2 (18-30 months) were used in this study fattened for three monthsgiven differentfeeds level, ie. P0 (100% native grass), P1(40% roughage + 60% concentrate), P2(15% of soybean pod+ 85% concentrate), and P3(30% soybean pod + 70% concentrate). Observed parameters included production of soybean waste per hektar, carry capacity of cattle based of soybean waste, income over feed cost (IOFC), andRC ratio.The results showed that the production of soybean pods and tofu waste was 1.75 ton/ha, and 0.44 ton/ha. This results indicated that carry capasity of soybean wastes for cattle production was of8,95 ST/ha/year, and 1,91 ST/ha/year respentively. Economy analisis based on income over feed cost and R/C ratio results showed that use of soybean waste was to replaces forage quite potensial for farmer, especially in the region to here soybean production is high.
Polymorphism of stearoyl-CoA desaturase (SCD1) gene in Indonesian local cattle Hilmia, N.; Noor, R. R.; Sumantri, C.; Gurnadi, R. E.; Priyanto, R.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 1 (2017): March
Publisher : Diponegoro University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (107.456 KB)

Abstract

Stearoyl-Coa desaturase (SCD1) gene is one of genes that involves in converting saturated fatty acids to unsaturated fatty acids. SNP at exon 5 in SCD1 gene that changes amino acid valine to alanine (V293A) has an influence to meat fatty acid composition. The aim of this research was to analyze SCD1 gene polymorphisms based on SNP V293A at exon 5 of three Indonesian local cattle. The identification of SCD1 gene polymorphisms was done by using 98, 20 and 7 DNA sample from Ciamis, Bali/Banteng, and Ongole Grade (PO) cattle, respectively. PCR_RFLP method with AciI enzim was carried out to identify SNP Val293Ala. Allelic frequencies and heterozygosity value were analyzed by using POPGENE32. The result showed that SCD1 gene at Ciamis local cattle and PO cattle were polymorphic. Their frequencies were 74.5% and 71.4% for T and 25.5% and 28.6% for C, respectively. There were three genotypes on Ciamis local cattle i.e TT, CT and CC with their frequencies were 52%, 44.9% and 3.10%, respectively. There were two genotypes on PO cattle i.e TT and CT with their frequencies were 42.9% and 57.1%, respectively. Meanwhile, SCD1 gene in Bali cattle was monomorphic. Heterozygosity value of SCD1 gene in Ciamis and PO cattle were 0.38 and 0.44, respectively. Their heterozigosities were categorized as medium. 
PRODUCTIVITY AND GENETIC DIVERSITY OF LOCAL CATTLE IN CIAMIS-WEST JAVA Hilmia, N.; Noor, R.R.; Sumantri, C.; Gurnadi, R.E.; Priyanto, R.
Journal of the Indonesian Tropical Animal Agriculture Vol 38, No 1 (2013): (March)
Publisher : Diponegoro University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (131.858 KB)

Abstract

The objectives of this study were to identify the productivity and genetic diversity of local cattle inCiamis West Java based on DNA microsatellite, in order to provide the basic information for its rearing,conservation and development. Eighteen of local cattle were kept for 58 days by feeding concentratesand rice straw. The measured parameters were weekly body weight as well as carcass percentage. Thepercentage of comparing carcass was taken from PO, Bali and crossbred cattle from local cattle withLimousin, Simmental, Brahman and Angus cattle. Data were analyzed using Excel software and SAS(2004). Blood samples were taken from two subpopulations i.e., 46 and 52 samples from Tambaksariand Cijulang areas, respectively. Other DNA samples were collected from Bali, PO, Limousin, Simental,Brahman and Angus cattle. Genetic diversity and phylogenetic analysis were analyzed using 3microsatellite loci, that were INRA35, HEL9 and BM2113. The genotyping data were analyzed usingPOPGENE.32 program, while phylogenetic trees were analyzed by MEGA 4. Average daily gain (ADG)of local cattle in Ciamis was 0.62±0.23 kg/head/day with a carcass percentage was 51.62±1.80%. Thecarcasses percentage of local cattle in Ciamis was not significantly different from those of Bali, PO, andcrossbred cattle. The genetic diversity of local cattle in Ciamis was categorized in the medium level, thediversity of local cattle in Tambaksari subpopulation was higher than in Cijulang subpopulation. Localcattle in Ciamis had a closer genetic distance with the PO cattle.
Prediction of meat quality in Bali cattle using ultrasound imaging Jakaria, J.; Khasanah, H.; Priyanto, R.; Baihaqi, M.; Ulum, M. F.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 2 (2017): June
Publisher : Diponegoro University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (214.666 KB)

Abstract

The objective of this study were to predict carcass quality characteristics in Bali cattle using ultrasound imagery. The Number of samples were 81 heads of Bali cattle consist of bulls (62 heads) and cows (19 heads) with various age ranging from 1 to 6 years were collected their body weight and carcass qualities including backfat thickness (BF), longissmus dorsi thickness (LD), rump fat thickness (RF), rump thickness (RT), marbling score (MS) and the percentage of intramuscular fat (PIF). Those were estimated using ultrasound performed on 4.5-6,5 MHz frequency with depth of 8.8-13 cm. The BF, LD, MS and PIF measurement were applied on 12th-13th ribs, while the RT and RF measurement were conducted between ischium and illium. MS determination was calculated using Aus-Meat standard, while PIF was analysis based on Deaton and Rouse (2000). Body weight and carcass quality among traits were analyzed using descriptive and correlation procedures. The results showed that performance of body weight and carcass quality differs between Bali bulls and Bali cows, as well as among the age variations. Correlation analyses among traits (body weight and carcass quality) showed strong positive correlation (P<0.05) ranging from 0.291 to 0.938. In conclusion, ultrasound imaging method could be used to estimate carcass quality characteristics in Bali cattle.
IDENTIFICATIONS OF CARCASS CHARACTERISTIC FOR ESTIMATING THE COMPOSITION OF BEEF CARCASS Hafid, H.; Gurnadi, R.E.; Priyanto, R.; Saefuddin, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 35, No 1 (2010): (March)
Publisher : Diponegoro University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (121.266 KB)

Abstract

The research aimed to identify carcass characteristic that can be used for estimating composition ofbeef carcass. It was used 165 Brahman crossbred cattle in this research. Carcass characteristics wereweight of a half cold carcass (WC) ranged from 96 to 151 kg, loin eye area (LEA) ranged from 22.09 to304.8 mm2, 12th rib fat thickness (FT12) ranged from 0.80 to 2.90 mm, meat ranged from 53.55 to 90.10kg and carcass fat ranged from 5.54 to 39.72 kg. Result showed that a half weight cold carcass as asingle indicator major may be used to predict meat weight and carcass fat with regression linearequation: Meat (kg) = 10.64 + 0.49 WC (R2=0.728 and SE=3.58), and Fat (kg) = -21.70 + 0.36 WC**(R2=0.582 and SE=3.65). Multiple regression to predict meat weight based on fat percentage of kidney,pelvic and heart (KPH) was Meat (kg) = 7.99 + 0.58 WC** - 4.41 KPH** (R2=0.751 and SE=3.43).
THE GROWTH PATTERNS OF CARCASS TISSUES WITHIN WHOLESALE CUTS IN FATTENING STEER Priyanto, R.; Johnson, E.R.; Taylor, D.G.
Journal of the Indonesian Tropical Animal Agriculture Vol 34, No 3 (2009): (September)
Publisher : Diponegoro University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (100.866 KB)

Abstract

An investigation was conducted on beef carcasses, aimed at identifying the growth patterns of wholesalecuts and their tissues in steer during fattening phase. This study involved 69 grass-fed steers with a liveweightrange of 300 to 600 kg. They had entered, or were progressing along, their fat deposition phase. Anallometric model (Huxley, 1932) was used to study the growth patterns of carcass tissues within wholesalecuts. In general, The growths of muscle and fat within wholesale cuts followed similar patterns. Somedifferences in growth patterns between muscle and fat were identified on the dorsal region. The growth impetusfor fat moved from the thoracic backwards to the lumbar region which was the reverse of the growth impetus formuscle. Differential growth patterns occurred between intermuscular (IM) fat and subcutaneous (SC) fat. WithIM fat, its growth movement was similar to that of total fat where there was a concentration of growth in thelumbar area and thin flank as side weight increased. With SC fat, there were growth movements from the ventralregion to the dorsal region of the carcass. Bone growth within wholesale cuts showed a less clear pattern.
Production and physical composition of Priangan male sheep carcass supplemented by Pasak Bumi peptide (Eurycoma longifolia Jack) ., Zubir; Priyanto, R.; Gurnadi, E.; Manalu, W.; Winugroho, H.M.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 3 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Original Source | Check in Google Scholar | Full PDF (370.145 KB)

Abstract

Pasak Bumi (Eurycoma longifolia Jack/ELJ) is frequently claimmed to increase expression of masculinity, including inducing the lean growth. This study was aimed to determine the effect of ELJ peptide supplementation on yield and physical composition of lamb carcasses. Sixteen Priangan rams about 1 year old with average weight of 30.43±1.41 kg were maintained in individual cages. The experimental rams were divided into 4 treatments i.e. T0, T1, T2, and T3. In T0, T1 and T2 treatments, the experimental rams were administered: 0, 1.5, and 3 mg ELJ peptide/kg BW, respectively. In the T3 treatment, the experimental ram were administered 1 mg/kg commercial product eurypeptide®. The ELJ peptide and commercial product were administered first in the morning prior to feeding. The experiment was conducted in a completely randomized design of four treatments and four replications. Data were covariance analyzed with an empty body weight as covariate for carcass weight and the right carcass weight as covariate for carcasses physical composition. The results showed that the weights of dissection body fat of T2 and T3 were lower than T0 (P &lt; 0.05) and T1 (P &lt; 0.01). Fat weight reduction was found in all parts of the carcass and more apparent in the fore saddle. Lean weights were higher ( P &lt; 0.05) in T3 and T2 as compared to T1 and T0. The pattern of lean weight differed among treatments in the wholesale cuts that follow the total lean weight eventhough it was not significant. ELJ peptide treatment did not significantly affect either bone weight or wholesale cuts weight. In conclusion, T2 and T3 can improve carcass composition with equal ability. Key Words: Ram, Eurycoma Longifolia Peptide, Carcass
SINGLE NUCLEOTIDE POLYMORPHISMS OF LIPOPROTEIN LIPASE GENE AND ITS ASSOCIATION WITH MARBLING QUALITY IN LOCAL SHEEPS Hidayati, H.; Sumantri, C.; Noor, R. R.; Priyanto, R.; Rahayu, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 1 (2015): March
Publisher : Diponegoro University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (522.929 KB)

Abstract

Lipoprotein lipase (LPL) is a key enzyme that plays in metabolism and transport lipoprotein andtherefore has an influence on blood triglyceride levels. LPL controls triacylglycerol partitioning betweenadipose tissue and muscle that increases fat storage or provides energy in the form of fatty acids formuscle growth. The research was aimed to explore Single Nucleotide Polymorphisms of LPL gene andto associate SNP with marbling quality. A total of 66 genomic DNAs consisted of sumatera thin-tail edsheep (50 heads) and garut sheep (16 heads) were used in this study. Polymerase Chain Reaction wasused to amplify genomic DNA and direct sequencing method was to identify polymorphism sequences.The sequences were analyzed with Bio Edit and MEGA 5.2. The BLAST sequence was obtained fromgene bank X.68308.1. The association between the genotype and marbling quality was analyze by oneway ANOVA and further between mean differences were tested using least sgnificant difference. Theresults showed that 3 novel SNPs i.e. insertion g.26>C; insertion g.27> G and c.192T>C on garut sheepand a SNP insertion g.26>C/G on sumatera thin-tail ed sheep. The diversity of LPL gene at c.192T>Cwas associated with heneicosanoic acid, whereas TT genotype (0.04%) was higher than CC (0.03%) andCT (0.02%).