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Mode of Action Temu Kunci (Kaempferia pandurata) Essential Oil on E. coli K1.1 Cell Determined by Leakage of Material Cell and Salt Tolerance Assays MIKSUSANTI, MIKSUSANTI; JENIE, BETTY SRI LAKSMI; PRIOSOERYANTO, BAMBANG PONTJO; SYARIEF, RIZAL; REKSO, GATOT TRIMULYADI
HAYATI Journal of Biosciences Vol 15, No 2 (2008): June 2008
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (44.681 KB) | DOI: 10.4308/hjb.15.2.56

Abstract

The essential oil of Kaempferia pandurata consist of terpen and oxygenated terpen that exhibits broad-spectrum antimicrobial activity. It’s mode of action against the gram-negative bacterium E. coli K1.1 has been investigated using a range of treatments. The mode action of the essential oil were analyzed by it’s ability to leakage E. coli K1.1 cell, to change permeability of the cell, and to alter salt tolerance of the cell. Ion leakage from the cell were analyzed by atomic absorption spectrophotometer. Salt tolerance assays was conducted by investigating the ability of E. coli K1.1 treated with temu kunci essential oil to grow on NA supplemented with NaCl. Protein and acid nucleic leakage were analyzed by UV spectrophotometer. There were inorganic compound leakage (potassium, calcium ion) and organic compound leakage (nucleic acid, protein) from cytoplasmic membrane, after exposing this organism to essential oil of Kaempferia pandurata. The more concentration of oil added, the more leakage was observed due to the loss of absorbing material such as nucleic acid (260 nm) and protein (280 nm), the loss of potassium and calcium ion, and loss of the salt tolerance of E. coli K1.1. Key words: essential oil, E. coli K1.1, leakage, salt tolerance assay
MINUMAN TINGGI AKTIVITAS ANTIOKSIDAN BERBAHAN DASAR ALAMI MINYAK BEKATUL PADI (Oryzae sativa) DAN EKSTRAK MENIRAN (Phyllanthus niruri) Rachman, Purnawati Hustina; Priskila, .; Damayanthi, Evy; Priosoeryanto, Bambang Pontjo
Jurnal Gizi dan Pangan Vol 7, No 3 (2012): November 2012
Publisher : Jurnal Gizi dan Pangan

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ABSTRACTThis study aimed to assess the antioxidant potential of chocholate-rice bran oil-emulsion beverage and meniran extract beverage. RBO beverage was made using oil in water emulsion with sugar ester as the emulsifier.Four formulas was created with the RBO-chocolate concentrate : water ratio of 1:1, 1:3, 1:5, and 1:7. Meanwhile the meniran beverage was made by adding extract meniran in five different concentrations (30, 40, 50, 60, 70 ppm) along with flavor and sucralose. The selected emulsion beverage had the AEAC value of 30.75 mg Vit C/100 g, while the meniran extract beverage had the AEAC value of 9.12 mg Vit C/100 g with the total fenol of 92.75 mg/100 g. The results of TBA value, content of protein, fat, carbohydrate, ash, and water of the emulsion beverage were 0.12 mg malondialdehyde eq/kg, 0.24%, 0.52%,6.74%, 0.15% and 92.32%, respectively. Increasing concentrations of chocolate and RBO significantly affected the results of the chemical parameters (p
Pengobatan penyakit tumor mammae melalaui operasi (matektomi dan ovariohisterektomi) dan kombinasinya (tanaman herbal) pada hewan S, Gunanati; Priosoeryanto, Bambang Pontjo; Wientarsih, Ietje; Sumarny, Ros
Jurnal Ilmu Pertanian Indonesia Vol 14, No 1 (2009): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Tumor or neuplasm can be meant as a abnormal and uncontrol growth of the transformation tissue or the change of one or main location of the body. This degenerative desease is one of the deseases in the animal pet especially dog and cat. Generaly this tumor deseases can be used with therapy using surgery and usually will relapse after six months. from our previous research had succeded to examp by using activity in vitro antiproliverati from extract plant (nusa indah, blustru and temu putih) combined with recombinanct interferon dog ( rCaiFN). The above phenomenon indicate a new hope to make a therapy for tumor desease, especially for dog and cat and may be in the future can be used for human. In this research we used 21 female rabbits and divided into 7 treatment groups, consisted of 3 rabits ie : group A. negative control; B. surgery, preventive andcurative curcumine; C. positive control; D. positive control and surgery; E. surgery and preventive zedoaria capsul; F. surgery , preventive and curative zedoaria capsule and G. surgery and curative zedoaria capsule . Induction with carcinogen (MNU) treated every weeks during 3 weeks. Surgery is executed in the 5 weeks and giving capsule zedoaria is executed every day for 4 weeks (preventive or curative) and 8 weeks (preventive andcurative). Result of the research indicated that mammae tumor has been made successfully by MNU (n-metil-nnitrosuria)induction to rabit and capsule zedoaria and it has been treated by doing surgery therapy (mastectomy and ovariohisterectomy). Also decombination of zedoaria capsule. The result of the research indicated lindrance of tumorgenesis to the group which is given by zedoaria capsule. from the clinical picture shows that zedoaria capsule does not give negative effect to clinical picture (temperature, respiratory frequency and heart rate)/ stillnormal to all the groups. Tumor induction with (MNO) at mammary gland will occur allegic reaction inflammatory which is the beginning of tumor mechanism which is marked by total leucosit, neutrofil, eosinofil, and basofil increase. Tumor therapy with surgery (masektomi and ovariohisterektomi) and the combination with rimpang Temu Putih capsule can decrease the number of leucosit, neutrofil and limfosit but only a little affectmonosit number, although statistically it does not show real difference.
EFEK KONSUMSI MINUMAN BUBUK KAKAO (Theobroma cacao L.) BEBAS LEMAK TERHADAP SIFAT ANTIOKSIDATIF LIMFOSIT SUBYEK PEREMPUAN [The Effect of Fat Free Cocoa (Theobroma Cacao L.) Powder Drinks Consumption on Antioxidative Activity of Lymphocyte of Women Subjec Erniati, .; Zakaria, Fransiska R.; Priosoeryanto, Bambang Pontjo
Jurnal Teknologi Dan Industri Pangan Vol 23, No 1 (2012): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (252.854 KB) | DOI: 10.6066/5299

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Ketersediaan Hayati Isoflavon dalam Plasma dan Pengaruhnya Terhadap Nilai Biokimia Darah pada Tikus Hiperglikemia (BIOAVAILABILITY OF ISOFLAVONE PLASMA AND THEIR EFFECT OF THE VALUE OF BLOOD BIOCHEMISTRY IN HYPERGLYCEMIC RAT ) Suarsana, I Nyoman; Widyastuti, Srikayati; Priosoeryanto, Bambang Pontjo
Jurnal Veteriner Vol 13, No 1 (2012)
Publisher : Jurnal Veteriner

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Isoflavone compounds have been known to have biological effects in improving health. The purpose ofthis study was to determine the bioavailability of isoflavone in plasma and its role as lowering bloodglucose levels in hyperglycemia rats. Bioavailability of isoflavones in plasma was performed in 18 ratsstrains Spraque Dawley age 3 months with 200 g of body weight on average and were given 300 ppm oralof isoflavone single dose. The analysis of isoflavon as done at 0, 1, 6, 12, 18 and 24 hours using HPLCmethod. Study effect on blood biochemical values used 25 male rats, which was divided into 5 treatmentgroups. One group as a negative control (K1) was given aquades; positive group hyperglycemia (K2), and 3other groups (K3, K4 and K5) were a positive groups of hyperglycemia and given isoflavone different doses,i.e. 1.0, 1.5 and 2.0 mg/200g bw per oral. Blood glucose were analyzed by using glucose oxidase biosensor,while cholesterol, HDL (Hight-density lipoprotein) and triglycerides were analyzed enzymatically using aspectrophotometer. The results showed the analyzes isoflavone genistein was more efficiently absorbedthan daidzein and glisitein. Isoflavone as found at highest concentration in plasma at 6 hours after oraladministration i.e: genistein of 11,17 ppm, daidzein of 2,86 ppm and glisitein of 2,47 ppm. Theadministration isoflavones at dose 2 mg/200g bw could decrease blood glucose levels in hyperglycemiarats, and also has hipocholesterolemia effect.
Antioxidant, Cytotoxic Activities and Total Phenolic Content of Four Indonesian Medicinal Plants Nurcholis, Waras; Priosoeryanto, Bambang Pontjo; Purwakusumah, Edy Djauhari; Katayama, Takeshi; Suzuki, Toshisada
VALENSI Vol 2, No 4 (2012): Jurnal Valensi Volume 2//No.4//Mei 2012
Publisher : Program Studi Kimia, Fakultas Sains dan Teknologi, UIN Syarif Hidayatullah Jakarta

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The crude ethanol extracts of four Indonesian medicinal plants namely Curcuma xanthorrhiza Roxb.,Phyllanthus niruri Linn., Andrographis paniculata Ness., and Curcuma aeruginosa Roxb. wereexamined for their antioxidant (radical scavenging) activity using 2, 2-diphenyl-2-picrylhydrazyl(DPPH) free radical and cytotoxicity using brine shrimp lethality test (BSLT). The total phenoliccontent was used the Folin-Ciocalteu method. IC50 values for DPPH radical scavenging activityranged from 14.5 to 178.5 μg/ml, with P. niruri having the lowest value and therefore the mostpotent, and C. aeruginosa having the highest value. LC50 values for BSLT ranged from 210.3 to593.2 μg/ml, with C. xanthorrhiza and A. paniculata having the lowest and highest values,respectively. The total phenolic content of the Indonesian plants ranged from 133.0 ±3.7 to863.3±54.7 mg tannic acid equivalent per 1 g extract, with C. aeruginosa and P. niruri having thelowest and highest values, respectively. A positive correlation between free radical scavengingactivity and the content of phenolic compounds was found in the four of Indonesian medicinal plants.
Karakteristik Nori dari Campuran Rumput Laut Ulva lactuca dan Eucheuma cottonii Zakaria, Fransiska Rungkat; Priosoeryanto, Bambang Pontjo; Erniati, Erniati; Sajida, Sajida
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 1 (2017): Juni 2017
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (98.218 KB) | DOI: 10.15578/jpbkp.v12i1.336

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AbstrakRumput laut jenis Ulva lactuca dan Eucheuma cottonii merupakan rumput laut yang dapat dijumpai di perairan Indonesia, akan tetapi pemanfaatannya sebagai produk nori belum dilakukan. Penelitian ini bertujuan untuk memproduksi nori dari rumput laut U. lactuca dan E. cottonii dan menguji karakteristik mutu fisik, kimia dan sensori. Dari hasil penelitian didapatkan bahwa campuran rumput laut U. lactuta dan E. cottonii dapat menghasilkan produk nori yang berkualitas. Hasil uji organoleptik menunjukkan produk nori yang dipanggang lebih dapat diterima dibandingkan nori tidak dipanggang. Karakteristik kimia dan fisik produk berbeda nyata pada taraf 5%. Hasil analisis karakteristik kimia menunjukkan bahwa produk nori rumput laut U. lactuca dan E. cottonii mempunyai kandungan protein yang lebih rendah (18.84%), kandungan karbohidrat yang lebih tinggi (62.31%), kandungan serat pangan yang lebih tinggi (36.76%) dan kapasitas antioksidan yang lebih rendah (43.01%) dibandingkan nori komersial (karbohidrat 41.8%, protein 40%, serat 21.3%, kapasitas antioksidan 51%). Characteristics of the Nori from Mixture of  Ulva  lactuca and Eucheuma cottonii SeaweedsAbstractUlva  lactuca and  Eucheuma  cottonii are seaweed that are available in Indonesia, but their utilization as food product is very limited. This study aimed to produce nori processed from mixture of  U.  lactuca and  E.  cottonii seaweed and evaluate the quality characteristics of the physical, chemical and sensory of the product. The results showed that a mixture of U. lactuca and E.  cottonii seaweed could be processed into the nori. Organoleptic tests showed that roasted nori product were more acceptable than unroasted nori. The chemical and physical characteristics of the product significantly different at the level of 5%. Chemical characteristics showed that the nori product from  U.  lactuca and  E.  cottoniiseaweed contained higher carbohydrate (62.31%), lower protein (18.84%), higher fiber content (36.76%) and slightly lower antioxidant capacity (43.01%) compared to commercial nori (carbohydrate 41.8%, protein 40%, fiber 21.3%, and antioxidant capacity 51%).
Ultrastructural and Immunohistochemical Studies of Transplanted Canine Lung Carcinoma Cell to Severe Combined Immunodeficiency Mice (STUDI ULTRASTRUKTUR DAN IMUNOHISTOKIMIA TRANSPLANTASI SEL KANKER PARU-PARU ANJING PADA MENCIT SEVERE COMBINED IMMUNODEFF Sari, Dwi Kesuma; Priosoeryanto, Bambang Pontjo; Agungpriyono, Dewi Ratih; Yamaguchi, Ryoji; Uchida, Kazuyuki; Tateyama, Susumu
Jurnal Veteriner Vol 14, No 4 (2013)
Publisher : Jurnal Veteriner

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Abstract

Primary lung cancers, or tumors originating in the lung, are relatively uncommon in dogs. The objectiveof this study was to describe the canine lung carcinoma that serially transplanted into severe combinedimmunodeficiency (SCID) mice, in order to established cell line from this tumor cell.  Morphology andcharacteristic of this canine lung carcinoma in SCID mice by histopathological and ultrastructuralexaminations with metastatic lesion in lung were also examined.  Histopathologically, the tumor masswere consisted of cuboidal to columnar cells with papillary pattern, uniform in size, the nuclei were oftenvariable in size, and some cells have vacuole on their cytoplasms.  Glandular forms were predominant withlobulated pattern, ductal pattern with papillary injected into tube-like structure were also encountered.Mitotic figures commonly found with inflammatory reaction were sometimes present in the interstitiumand lumen gland.  Ultrastructural analysis of the tumor cells showed round to oval cells with one or moreprominent nucleoli.  The cells possessed numerous mitochondria, smooth endoplasmic reticulum, andindividual cells which were interconnected via desmosomes.  Tonofilament characterize by long cytoplasmicmaterial was encountered.  Positive reaction of the round to oval tumor cells to anti keratin antibodyconfirmed that their epithelial cell nature.  Lung metastatic lesions were found in SCID mice aftertransplantation and this phenomenon indicated that canine lung carcinoma is tumorigenic to SCID mice.
PERANAN HEMAGLUTININ Staphylococcus aureus DALAM PROSES ADHESI PADA SEL EPITEL AMBING SAPI PERAH Abrar, Mahdi; Wibawan, I Wayan Teguh; Priosoeryanto, Bambang Pontjo; Soedarwanto, Mirnawati; Pasaribu, Fachriyan Hasymi
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (352.365 KB) | DOI: 10.21157/j.ked.hewan.v7i1.564

Abstract

Tujuan penelitian ini adalah mengetahui kemampuan hemaglutinin pada Staphylococcus aureus (S. aureus) dalam proses adhesi. Uji adhesi pada sel epitel ambing dilakukan dengan menggunakan metode Velentine-Weiggand. Hasil penelitian ini menunjukkan bahwa S. aureus yang memiliki hemaglutinin menunjukkan kemampuan adhesi yang jauh lebih baik (±380 bakteri/20 sel) dibandingkan dengan yang tidak memiliki hemaglutinin (±56 bakteri/20 sel).
Dyspepsia in Nonsteroidal Anti-inflammatory Drugs Gastropathy Manan, Chudahman; Priosoeryanto, Bambang Pontjo; Daldiyono, Daldiyono; Estuningsih, Sri; Rahminiwati, Min
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 12, NUMBER 2, August 2011
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

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Abstract

Background: Nonsteroidal anti-inflammatory drugs (NSAIDs) gastropathy is a common complication, which has characteristic symptoms of dyspepsia syndrome. Mostly, it includes epigastric discomfort with bloating and nausea. The aim of this study was to provide evidences that clinical symptoms of dyspepsia are related to macroscopic changes of gastric form in rats, which are expected to be applied in human. Method: The study was conducted in 20 white rats (Rattus norvegicus, Sprague-Dawley strain) at the Department of Pathology and Clinical Reproduction, Bogor Agricultural University between January and December 2008. The rats were divided to treatment group and control group and each group consisted of 10 rats. Acetyl salicylic acid (ASA/aspirin) was administered at 400 mg dose, diluted in distilled water and was given to the treatment group using gastric cannula, once daily for three days period; while the control group had received aquabidest only. Subsequently, necropsies were conducted for both groups, followed by macroscopic observation and measurement of sagittal and transversal diameter. Gastric incisions along the minor curvature were performed in both groups to recognize any macroscopic changes of gastric mucosa. ANOVA test was utilized for data analysis, which was followed by Duncan test when the results were significant. Results: Gastric diameters in treatment group with positive lesion were significantly different from the control group and the treatment group with negative lesion on anthrum/pylorus region, with p < 0.05. Conclusion: Prominent gastric dilatation at anthrum/pylorus region found in the treatment group may become the initial cause and signs of dyspepsia in human. Keywords: NSAID gastropathy, dyspepsia, NSAIDs/aspirin, gastric dilatation