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Isolasi dan Determinasi Bakteri Luminesensi yang Bersimbiosis pada Cumi-cumi Loligo duvauceli Pringgenies, Delianis; Sejati, Sri
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 9, No 1 (2004): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Bioluminisensi merupakan penomena alam, yaitu cahaya yang ditimbulkan oleh suatu organ sebagai hasil dari reaksi kimia yang melibatkan tiga komponen, yakni luciferin (substrat), luciferase (enzim) dan molekul oksigen. Hasil penelitian menunjukkan bahwa bakteri simbiosis pada organ luminisensi cumi L. duvauceli adalah bakteriluminisen dari jenis bakteri Photobacterium phosphoreum dan bakteri memancarkan warna biru. Jenis bakteri ini merupakan bakteri yang paling terang memancarkan cahaya di bandingkan dengan jenis bakteri luminisensi lainnya. Hasil penelitian terhadap jumlah total bakteri memperlihatkan bahwa bakteri dapat memancarkan cahaya pada konsentrasi 4,6. 109 CFU/ml dengan diameter koloni bakteri pada 0,075 cm, sedangkan pada konsentrasi lebih rendah yaitu 2,0. 104 dengan diameter koloni bakteri 0,025 cm memperlihatkan bakteriluminisensi tidak memancarkan cahaya. Jadi konsentrasi bakteri sangat berperan dalam proses pemancaran cahaya pada bakteri luminisensi P. phosphoreum.Kata kunci: Isolasi bakteri, luminesensi, cumi-cumi (Loligo duvauceli)Bioluminescence refers to the visible light emission in liveng organisms that accompanies the oxidation of organic compounds (luciferins), mediated by an enzyme catalyst (luseferase). Result on the identification of bacteria live in the cuttlefish of L. duvauceli showed species of Photobacterium phosphoreum and the colony emits a blue lights. P. phosphoreum is the brightest bacteria in terms of emitting lights compared to other species of luminous bacteria. The total bacterial count gives 4.6 109 CFU/ml with diameter of colony of 0.075 cm which able to emit lights, while lower concentration at 2.0 104 CFU/ml and colony diameter of 0.025 cm give no emission of light. In respect to this, it suggest that concentration of luminous bacteria has role in theprocess of light emission by P. phosphoreum.Key words: Bacteria isolation, bioluminescence bacteria determination, squid (Loligo duvauceli)
Morfologi dan Anatomi Cumi-Cumi Loligo duvauceli yang Memancarkan Cahaya Rudiana, Esti; Pringgenies, Delianis
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 9, No 2 (2004): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Setiap makhluk hidup di alam harus mempunyai strategi agar dapat lulus hidup dan strategi tersebut berbedabeda untuk setiap individu. Salah satu strategi cumi-cumi dalam mempertahankan diri dengan cara memancarkan cahaya, oleh karena itu ada beberapa jenis cumi-cumi yang memiliki organ cahaya. Tujuan penelitian ini adalah mengkaji morfologi dan anatomi cumi-cumi Loligo duvauceli yang dapat memancarkan cahaya. Pemecahan masalah dalam penelitian ini dilakukan melalui aspek cumi-cumi yang ditangkap langsung darilait Jepara dan dianalisis morfologi dan anatominya. Hasil penelitian menunjukkan bahwa jenis cumi-cumi yang memancarkan cahaya di perairan Jepara dan sekitarnya adalah Loligi duvauceli yang memiliki sepasang organ cahaya menempel pada dorso-lateral kantung tinta. Organ cahaya cumi berbentuk bulat, sebagian terdapat pada permukaan dan sebagian terbenam pada dinding kantung tinta. Organ cahaya terdiri atas lensa yang terletak pada permukaan luar kantung tinta, dan kantung organ cahaya (terbenam pada dinding kantung tinta) yang mempunyai saluran penghubung kantung dengan rongga mantelKata kunci: cumi-cumi (Loligo duvauceli), morfologi, anatomi, organ cahayaEvery organism in nature has a strategy in order to survive and that strategy may be deferent on each organism. Squids escape from predators by emitting lights; therefore, squids have to develop an organ in order to be able to emit light. Squids were collected from Jepara water and adjacent, the observation was done by analyzing of morphology and anatomy of squid. Results from identification of squids live in Jeparawaters and the adjacent is Loligo duvauceli. This species has a pair of light organs attached at the dorsalateral of the ink sac. The light organ of the squid has a rounded shape, which is partly emerge on the surfaceand the other part was submerge in the wall of the ink sac. Light organ consists of a lens situated on the outer part of the ink sac and it has a connecting canal to the mantel holeKey words : squid (Loligo duvauceli), morphology, anatomy, light organ
Penapisan Bakteri Simbion Gastropoda Stramonita armigera Penghasil Senyawa Antibakteri Multi Drug Resistant dari Perairan Ternate Pringgenies, Delianis; Dananjoyo, Mijil Ciptaning
Jurnal Natur Indonesia Vol 13, No 3 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.733 KB) | DOI: 10.31258/jnat.13.3.200-206

Abstract

Antibiotic resistance is an ability of bacteria to hold the antibiotic effect. It was reported that there is a human-patogen bacteria that resistance to one or more classes of antibiotic. It become a problem on medical world. Tosolve those problems, it is necessary to search the new antibiotic compounds that more effective and efficient tosolve the problem of Multi Drug Resistance (MDR). The secondary metabolite-producing marine invertebrates andsymbiont microorganisms, have prospect as an antibiotic. The symbiont microorganisms may produce thesecondary metabolite similar to their host. The aims of the reseach were to determinate of gastropods symbiontbacteria that capable of producing Antibacterial MDR (Multi Drugs Resistant) Compound. Sample of Molusc werecollected from Ternate (Molucas) islands. Isolation of symbiotic bacteria, screening for bacteria which producingsecondary metabolites as anti-MDR bacteria, antibacterial test, isolation of clinical pathogenic bacteria (MDR),conducting anti-bacterial sensitivity test, sensitivity test for antibacterial, DNA exctraction, DNA amplificationbased on PCR method, DNA sequencing. Result of 16S r-DNA sequence was then analyzed and edited usingGENETYX program and followed by 16S rDNA sequence analysis. The result showed that 17 strains were isolatedfrom gastropods Stramonita armigera. Antibacterial assays showed that TSA 8.7 isolate have ability to inhibitPseudomonas sp., Escherichia coli dan Enterobacter sp. the molecular analyses showed that isolate TSA 8.7closed by related to Vibrio sp. Strain JZDN1, with 98% of homology. Based on this experimental result, it could beconcluded that gastropods-symbiont bacterium Stramonita armigera capable of producing antibacterial compoundagainst strain Multi Drug Resistant (MDR). There is 11 isolates of gastropods-symbiont bacteria Stramonita armigerathat have an antibacterial MDR activity.
STUDY TO INVESTIGATE INDUCED SPAWING METHOD AND LARVAL REARING OF THE SEA CUCUMBER Holothuria scabra *) Hartati, Retno; Pringgenies, Delianis
JOURNAL OF COASTAL DEVELOPMENT Vol 1, No 3 (1998): Volume 1, Number 3, Year 1998
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

In irder to increase natural population stocks, a sea cucumber cultivation project was attempted in the Karimunjawa Islands (Java Sea, Indonesia). Since continuous production of larvae is essential to such a project, this portion of the research focuses on methods of inducing the sea cucumber Holothuria scabra to spawn and monitoring the development of resulting larvae. Four different types of environmental manipulation were used to try and induce spawning; artificial fertilization (using manually extracted eggs and sperm), thermal shock (raising the temperature of the spawning medium), desiccation (partially drying out) and treatment with potassium chloride (KCL) solutions of various concentrations. The development of the resulting larvae was carefully observed. Results indicate that thermal shock, desiccation and potassium chloride (KCL) are all effective in stimulating the sea cucumber Holothuria nobilis to spawn. Thermal shock gave the best result with both male and female spawning and 90% larvae development. Using a stocking density of 300 larvae/ liter in 10 liter aquaria, larvae was successfully raised up to pentactula stage. Regardless of the method used to induce spawning, fertilizer eggs development into auricularia larvae at 31 hours and 30 minutes. These larvae then metamorphosed into doliolaria and pentactula larvae at day 13 and 26. Mortality of all stocks at the pentactula stage was probably due to lack of provision of settlement substance.
Potensi Antibakteri Ekstrak Rumput Laut Terhadap Bakteri Penyakit Kulit Pseudomonas aeruginosa, Staphylococcus epidermidis, dan Micrococcus luteus Siregar, Angelina Ferawaty; Sabdono, Agus; Pringgenies, Delianis
Journal of Marine Research Vol 1, No 2 (2012): Journal of Marine Research
Publisher : Journal of Marine Research

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Abstract

Along with the increasing resistance of bacteria in the health world, it is necessary discover a new drug. Sources of new antibacterial bioactive compounds can be obtained from seaweeds. The purposes of the research were to determine the antibacterial activity of crude extract seaweed againts the skin disease bacteria, Pseudomonas aeruginosa, Staphylococcus epidermidis and Micrococcus luteus, and to identify classes of compounds contained in crude extract of seaweed. This research was done from January to Mei 2012. Seaweeds sampling (Caulerpa sp, Eucheuma sp, Gracilaria sp dan Sargassum sp) was conducted in territorial water of Jepara. Extraction process and analysis of bioactive compound of crude extracts was conducted at Laboratory, Marine Science Department, Diponegoro University, Semarang. Test antibacterial activity was conducted at Medicine Laboratory, Ungaran. The method used in this research was experimental laboratories. Extraction process was conducted with solid-liquid extraction method. Extract were tested for antibacterial activity by agar diffusion method according to Kirby-baurer. Based on the antibacterial activity’s assay, 12 extracts which have antibacterial activity against test bacteria P. aeruginosa, S. epidermidis and M. luteus were obtained the 12 seaweed extracts the highest consentration of inhibition zone was 200 µg/disk in ethyl acetate extract of the Sargassum sp was the most active extracts against bacteria P. aeruginosa and M. luteus, while the methanol extract of Sargassum sp was the most active extracts against the bacteria S. epidermidis. Steroids was the most dominant compounds on all of 12 seaweed extracts.
Uji Fitokimia dan Aktifitas Anti Bakteri Ekstrak Media Supernatan Bakteri Simbion Vibrio sp. Gastropoda Oliva vidua Terhadap Bakteri Multi Drug Resistant Harmawan, Adityo; Ridho, Ali; Pringgenies, Delianis
Journal of Marine Research Vol 1, No 1 (2012): Journal of Marine Research
Publisher : Journal of Marine Research

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Abstract

The development of antibiotic resistance has become an important issue in medicine. Many types of bacteria are pathogenic to humans were reported to have become resistant to one or more types of antibiotics. It is pushing for a new search for alternative sources of antibiotic-producing compounds. One source of potential alternatives to be developed as a source of new antibiotic compound s are symbiotic bacterium with gastropods. The purpose of this study was to determine the characteristics of bacterial symbionts in gastropods ( TOV 12.16 bacterial isolates), the bioactivity of crude bacterial extracts against bacterial MDR and find a group of compounds from TOV 12.16 crude bacterial extracts which most potentially. The main materials used in this study were samples of bacterial isolates TOV 12.16 in isolation from the gastropods (Oliva vidua) from Aquatic Ternate (North Maluku). While the bacterium MDR test are Escherichia coli and Staphylococcus aureus obtained from the Laboratory of Microbiology Faculty of Medicine UNDIP. Biochemical tests used to determine the characteristics of bacterial isolates TOV 12.16. Meanwhile, to identify the bioactive compounds using phytochemicals screening and GC/MS. Bacterial symbionts Oliva vidua Gastropoda is Vibrio ordalii. Ethyl acetate crude extract of bacterial symbionts Oliva vidua Gastropoda species showed antibacterial activity of the most active against MDR bacteria test. Phytochemical screening test results showed the ethyl acetate crude extract showed two groups of compounds which contain flavonoids and triterpenoid compound that has both the antibacterial activity. Being on the analysis of the GC/MS_are_known_isobutyric_acid_and_2-metilbutanoic_acid.
Uji Toksisitas Ekstrak Kloroform Cangkang dan Duri Landak Laut (Diadema setosum) Terhadap Mortalitas Nauplius Artemia sp Aprilia, Hilda Ayu; Pringgenies, Delianis; Yudiati, Ervia
Journal of Marine Research Vol 1, No 1 (2012): Journal of Marine Research
Publisher : Journal of Marine Research

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Abstract

Eggshells and spines of sea urchins (Diadema setosum) have toxins that can lead to certain biota surrounding death. Presumably the sea urchin has toxic compounds that can affect the surrounding environment. Because it needed a way to determine the toxic properties of sea urchin. Bioactive compounds contained in a previous study showing shells and spines of certain species of sea urchin pigment is coated by a black liquid that is stable. This fluid can be used as a mesh and skin coloring. It can also be used as fertilizer. This study aims to determine the lethal toxicity (LC50-24 h) of the chloroform extract of sea urchin of mortality Nauplius Artemia sp. The method used in this study is the experimental laboratory. Chloroform extract of the eggshells and sea urchin spines were tested using animal toxicity tests Nauplius Artemia sp.The results showed that the lethal toxicity (LC50-24 h) of the Nauplius Artemia sp in the chloroform extract of sea urchin eggshells at 133.58 ppm. As for the chloroform extract of sea urchin spines obtained results for 168.178 ppm. Chloroform extracts of both eggshells and sea urchin spines have potential activity as anti-microbial bio
Uji Fitokimia dan Aktivitas Antijamur Ekstrak Teripang Keling (Holoturia atra) Dari Pantai Bandengan Jepara Terhadap Jamur Candida albicans Septiadi, Tedi; Pringgenies, Delianis; Radjasa, Ocky Karna
Journal of Marine Research Vol 2, No 2 (2013) : Journal of Marine Research
Publisher : Journal of Marine Research

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Abstract

Holothuria atra is one of the marine lifes that lives at the bottom of the substrate, and able to adapt to its environment. Several previous studies showed that extracts of H. atra from different waters have activity as an antifungal. Fungus Candida albicans is one of human pathogens that attack on the mucosa of the mouth, skin and vagina. The purpose of this study were to identify the compounds contained in the extracts of H.atra and examine the effect of extracts concentration against C. albicans. The process of extraction was done by maceration with solvent n-hexane, ethyl acetate and methanol. Testing of secondary metabolites was carried out using phytochemical screening methods while testing antifungal activity was using agar diffusion test. The results showed that the extracts of H.atra contained saponins, alkaloids, steroids and triterpenoids. Antifungal activity assays showed that the n-hexane extract did not show any inhibition zone, while the ethyl acetate and methanol extracts showed inhibition zone at a concentration of 1 mg / disk with a large zone of inhibition of 8.27 ± 0.06 and 8.07 ± 0 , 12 mm, respectively based on these results it can be concluded that the extract of ethyl acetate solvent H.atra has strong potential as antifungal.
Uji Fitokimia Dan Toksisitas Ekstrak Kasar Gastropoda (Telescopium telescopium) Terhadap Larva Artemia salina Putri, Mukti K. Diana; Pringgenies, Delianis; Radjasa, Ocky Karna
Journal of Marine Research Vol 1, No 2 (2012): Journal of Marine Research
Publisher : Journal of Marine Research

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Abstract

Gastropod is one of the marine organisms that can be used as a source of natural bioactive compounds, one of which is Telescopium telescopium. T. telescopium until now it has not been yet exploited optimally, some people still use it as feed ingredients that contain high protein nutrition, however it must be supported by scientific information about the efficacy and side effects. This research aims at determining the secondary metabolite by crude extract T. telescopium, and the lethal toxicity value (LC50-24 hours). The research consisted of two steps, namely the phytochemical and toxicity test. The method used exploratif and experimental laboratory with a complete randomized design, which consited of five concentration treatments namely 25.12, 63.1, 158.5, 398.15, and 1000 ppm with three replications. Each treatment used 10 Artemia salina larvae, and observations were made during 24 hours of Artemia salina larvae mortality. LC50 was determined by probit analysis using the EPA Probit Analysis Program Version 1.5 Finney. The results of the phytochemical test showed that crude extract T. telescopium contain alkaloid compounds, steroids, flavonoids. The results BSLT (Brine Shrimp Lethality Test) of crude extract T. telescopium LC50-24 hours showed that the crude extract clorofom (229.562 ppm), crude extract ethyl acetate (244.906 ppm), and crude extract methanol (197.242 ppm). LC50-24 hours < 1000 ppm showed that the crude extract Telescopium telescopium potent antitumor compounds.
Karakterisasi Metabolit Sekunder Bakteri Simbion Gastropoda Conus miles dengan Metode GC-MS Sebagai Antibakteri MDR (Multi Drug Resistant) Hasanah, Nurul Fitrah; Pringgenies, Delianis; Wulandari, Sri Yulina
Journal of Marine Research Vol 1, No 2 (2012): Journal of Marine Research
Publisher : Journal of Marine Research

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Abstract

It is known that the bacterial symbionts are symbiotic with gastropods Conus miles has the potential to be able to produce antibacterial compounds against bacterial MDR (Multi Drug Resistant). Information found previously from bacterial symbionts and isolation based on screening results, the size of the zone of inhibition and inhibitory properties against several types of bacteria tests showed that isolates derived from Conus miles Gastropoda symbionts characterized by kinship rate of 98% in bacteria Pseudoalteromonas sp. The research objective was to determine the characteristics of the bacterial symbiont bioactive compounds associated with the type of mollusk Conus miles. The study was conducted three phases namely the isolation and purification of bacteria; characterization of isolates identified through test results on the reaction of physiological and biochemical tests, as well as the identification of secondary metabolites by GC-MS method QP2010S Shimadzu. The results showed that the bacterial symbiont isolates had a number of compounds with a percentage of the highest peak to the lowest compound the compound, Acetic acid (CAS) ethylic acid (35.22%); propanoic acid, 2-methyl-(CAS) Isobutyric acid (11.78%); Iso -valeric acid (9:38%), butanoic acid, 2-methyl-(CAS) 2-methylbutanoic acid (4.74%) and 1,2-Benzenedicarboxylic acid, dioctyl ester (CAS) Dioctyl phthala (4:36%). The results concluded that the bacterial symbiont isolates obtained in this study provide information on the results of potentially new antibiotics.