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Journal : Jurnal Perikanan Universitas Gadjah Mada

ISOLASI DAN KARAKTERISASI BAKTERI PENDENITRIFIKASI YANG DIISOLASI DARI LUMPUR KAWASAN MANGROVE Triyanto, Triyanto; Isnansetyo, Alim; Prijambada, Irfan D.; Widada, Jaka; Kembaren, Duranta D.
Jurnal Perikanan Universitas Gadjah Mada Vol 10, No 1 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.8912

Abstract

The aim of this research is to isolate denitrifying bacteria which have the highest activity to reduce nitrate. The sources of the denitrifying bacteria were mangrove sediment collected from Cilacap Regency, Central Java and Indramayu Regency, West Java. Basalt medium containing KNO3 as a source of nitrogen was used for isolating the denitrifying bacteria. Double layer agar was used for making anaerob condition. Fourty-one isolates were obtained at the first step of the isolation, 29 of them have nitrate reduction activity at a range of 0.77-95.62%. Three isolates, i.e. D19.2, DR2.1 and D27.3 having the highest activity were selected for further examination. The selected isolates were characterized and identified. Characterization includes colony and cell morphology, Gram staining, motility, spore staining and biochemical tests as catalase and oxidase. Identification was done by using profile matching to Bergey’s Manual of Determinative Bacteriology. The results indicate that isolate D19.2 and D27.3 have similarities to the characters of genus Listeria, whereas isolate DR2.1 has similarities to the characters of genus Propionibacterium. All of the selected isolates were able to grow in a medium having NaCl concentration at a range of 0.5-3.5% and pH range of 5-8. Observation of nitrate reduction ability of the isolates during five days incubation shows that isolate DR2.1 has the highest denitrifying activity. The selected isolates can be used as bioremediation agents for controlling nitrate pollution in brackish water pond.
ISOLASI, KARAKTERISASI DAN UJI INFEKSI BAKTERI PROTEOLITIK DARI LUMPUR KAWASAN HUTAN BAKAU Triyanto, Triyanto; Isnansetyo, Alim; Prijambada, Irfan D.; Widada, Jaka; Tarmiawati, Afi
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 1 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.307 KB) | DOI: 10.22146/jfs.2966

Abstract

This research was aimed to isolate proteolytic bacteria that have high ability to hydrolize protein. The sources of bacterial samples were collected from mangrove sediment in Indramayu and Cilacap Regency. Collwel SWYE Modification Agar medium and Skim Milk Agar Medium, which contain pepton and casein, were used for isolating the bacteria. Forty isolates having proteolitic activity were obtained. Three isolates, i.e. Ps.23.1, Ps.27.3 and Ps.23.2, having the highest activity were selected. The selected isolates were able to grow at pH range of 5 to 8. They were also able to grow at NaCl concentration of 0.5-3.5%. Intramuscular injection test on white shrimp (Lithopenaeus vannamei) shows that Ps.23.1 and Ps.23.2 were not pathogenic to shrimp. On the other hand, Ps.27.3 was indicated to cause disease on shrimp. These results indicated that isolates Ps.23.1 and Ps.23.2 potentially can be used as inoculant for bioremedation of brackish water pond. Isolate Ps.23.1 was characterized to be an aerobic, nonmotile, short rod, gram negative bacteria having catalase and oxidase activities. It produced yellowish, circulair, entire colonies. Isolate Ps.23.2 was characterized to be an aerobic, motile, rod, spore forming, gram positive bacteria having catalase and oxidase activities.