Tri Heru Prihadi
Badan Riset Kelautan dan Perikanan, Pasar Minggu Jakarta Selatan

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Identifikasi dan Penentuan Kadar Senyawa Fenol Pada Sedimen Tambak Di Kabupaten Sidoarjo Sukandar, Dede; Prihadi, Tri Heru; Hayati, Ai Faziah
VALENSI Vol 1, No 4 (2009): Jurnal Valensi Volume 1//No.4//Mei 2009
Publisher : Program Studi Kimia, Fakultas Sains dan Teknologi, UIN Syarif Hidayatullah Jakarta

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Abstract

Analisis terhadap kadar dan jenis senyawa fenol dalam sedimen tambak yang tercemar dan tidaktercemar oleh air lumpur Lapindo di Sidoarjo, Jawa Timur, telah dilakukan. Penentuan kadarsenyawa fenol dilakukan dengan metode kolorimetri menggunakan reagen Folin Ciocalteau dandianalisis dengan UV-Vis pada panjang gelombang 740 nm. Kadar senyawa fenol yang terdapatdalam tambak tercemar dan tidak tercemar air lumpur Lapindo berbeda secara signifikan. Senyawafenol lebih banyak terdistribusi pada tambak yang tercemar oleh air lumpur Lapindo. Kadarsenyawa fenol yang terdapat dalam masing-masing sampel rata-rata < 1 mg/L. Berdasarkan hasilanalisis secara kualitatif dengan GC-MS, jenis senyawa fenol yang teridentifikasi adalah fenol, 4-klorofenol, butilfenol dan metilfenol. Keempat senyawa tersebut merupakan jenis pencemar yangberbahaya bagi lingkungan perairan.
Analisis Kadar Phosfat dan N-Nitrogen (Amonia, Nitrat, Nitrit) pada Tambak Air Payau akibat Rembesan Lumpur Lapindo di Sidoarjo, Jawa Timur Hendrawati, Hendrawati; Prihadi, Tri Heru; Rohmah, Nuni Nurbani
VALENSI Vol 1, No 3 (2008): Jurnal Valensi Volume 1//No.3//November 2008
Publisher : Program Studi Kimia, Fakultas Sains dan Teknologi, UIN Syarif Hidayatullah Jakarta

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Abstract

Tujuan penelitian ini untuk mengetahui kadar Phosfat dan N-Nitrogen (Amonia, Nitrit, Nitrat)pada tambak air payau akibat rembesan lumpur Lapindo di Sidoarjo. Penentuan lokasi dilakukanpada tambak air payau yang tercemar dan tidak tercemar lumpur panas di Sidoarjo. Lokasitambak berada pada arah utara dan selatan pusat semburan lumpur Lapindo, tepatnyadisepanjang kali Alo dan kali Porong. Pada setiap tambak, diambil 5 sampel air yaitu inlet,outlet dan 3 titik ditengah tambak. Penelitian dilakukan pada bulan Mei - Juni tahun 2007metode yang digunakan mengacu pada SNI (Standar Nasional Indonesia) dengan menggunakanalat Spektrofotometer UV-Vis. Kadar amonia pada tambak yang tercemar lumpur kurang dari 2mg/L dan untuk tambak tidak tercemar kurang dari 3 mg/L. Kadar nitrit pada tambak yangtercemar antara 0- 27,86 mg/L dan untuk tambak tidak tercemar antara 0 – 0,22 mg/L. Kadarnitrat pada areal tambak tercemar 0 – 1,7 mg/L. Untuk tambak tidak tercemar disepanjang kaliPorong memiliki kisaran antara 0 – 0,3 mg/L. Kadar phosfat pada tambak tercemar antara 0,11-0,35 mg/L. Secara umum, kadar phosfat dan n-nitrogen pada tambak tercemar dan tidaktercemar menunjukkan perbedaan yang tidak berarti. Tetapi, dibandingkan dengan PP No.82tahun 2001, kadar amonia dan nitrit masih berada diatas ambang batas.
EFEKTIVITAS MINERAL KALSIUM TERHADAP PERTUMBUHAN YUWANA UDANG GALAH (Macrobrachium rosenbergii) Hadie, Lies Emmawati; Hadie, Wartono; Prihadi, Tri Heru
Jurnal Riset Akuakultur Vol 4, No 1 (2009): (April 2009)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.2 KB) | DOI: 10.15578/jra.4.1.2009.65-72

Abstract

Pertumbuhan udang galah dibatasi oleh kulitnya yang bersifat tidak elastis, karena terdiri atas khitin. Agar udang galah tumbuh dengan baik, maka harus ada unsur mineral dalam pakannya. Salah satu mineral yang bersifat esensial adalah mineral kalsium. Kalsium mempunyai fungsi dalam pembentukan tulang, jaringan lunak, proses regulasi dalam tubuh, dan menjaga keseimbangan asam basa. Oleh karena peran penting dari kalsium tersebut, maka dilakukan penelitian mengenai efek mineral kalsium dalam ransum pakan udang galah terhadap pertumbuhannya. Hewan uji yang digunakan pada penelitian ini adalah yuwana udang galah dengan kisaran bobot 56,0 ± 3,0 mg. Rancangan penelitian menggunakan Rancangan Acak Lengkap dengan perlakuan yang diterapkan adalah kalsium 1,0%; 3,0%; 5,0%; 7,0%; dan 0,0% sebagai kontrol. Setiap perlakuan mendapat 3 ulangan. Hasil penelitian ini menunjukkan bahwa kadar kalsium dalam ransum pakan sangat mempengaruhi laju pertumbuhan harian udang galah (P<0,05). Kadar kalsium yang optimal dalam ransum pakan udang galah adalah sebesar 3,46%.The growth of giant prawn is limited by a non elastic material called chitin, which is a limiting factor in its growth. Feed containing mineral is needed to improve its growth. One of the essential minerals is calcium. The function of calcium is essential in bone and soft tissue formations, acid balancing, and regulation processes in the body. Because of its benefits, the research on the calcium effect on giant prawn was conducted. The aims of this study was to know the effect of calcium on the growth rate of giant prawn juvenile. Test animals were juveniles of giant prawn with average weight of 56.0 ± 3.0 mg. Research design employed complete randomized design with five calcium mineral treatments as follows:1.0%, 3.0%, 5.0%, 7.0%, and 0.0% as control. Each treatment has three replications. The result showed that calcium affected the daily growth rate of giant prawn (P<0.05). The calcium dosage of 3.46% is the optimum level for giant prawn juvenile.
THE APPLICATION OF LIFE CYCLE ASSESSMENT TO EVALUATE WATER QUALITY CONDITION AROUND FISH CAGES IN CIRATA RESERVOIR, INDONESIA Prihadi, Tri Heru; Saputra, Adang; Taufik, Imam; Ardi, Idil
Indonesian Aquaculture Journal Vol 12, No 2 (2017): (December, 2017)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (347.171 KB) | DOI: 10.15578/iaj.12.2.2017.83-91

Abstract

Life Cycle Assessment (LCA) is an analytical tool used primarily for evaluating environmental conditions. The sources of decomposed organic matters in Cirata Reservoir originate from industrial activities, household waste, agricultural waste, and effluent from floating fish net cages. The wastes consist mainly of fat, protein, and carbohydrate. Bacteria are responsible for aerobic decomposition process of organic matters in the sediment. As bacteria consume oxygen during the decomposition processes, significant depletion of dissolved oxygen level in the waters may occur. This happens in Cirata Reservoir where a low level of oxygen in the water leads to anaerobic decomposition processes at the bottom of the reservoir. The porpuse of this study was to evaluate Cirata Reservoir water conditions, in terms of water quality, organic sediment and organic materials level based on the application of LCA. In this study, water and sediment samples were collected. Water quality conditions were measured in-situ. Water and sediment samples were analyzed in the laboratories. The results of the analyses showed that water quality condition in all sampling stations was relatively homogeneous. Based on the level of decomposition of organic matter, water quality conditions in Cirata reservoir could be classified as Class I, II, and III in IKA_STORET scale and categorized as poor. DO, sulfide, phenol, BOD, COD, total phosphate were outside the ranges of acceptable water quality standards.
IMPACT OF HEAVY METAL TO FISH AQUACULTURE IN FLOATING NET CAGE IN CIRATA RESERVOIR, INDONESIA Prihadi, Tri Heru; Murniyati, Murniyati; Ardi, Idil
Indonesian Aquaculture Journal Vol 3, No 1 (2008): (June 2008)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (440.524 KB) | DOI: 10.15578/iaj.3.1.2008.69-76

Abstract

Utilization of Cirata Reservoir for fisheries aquaculture with floating net cage system has been increasing rapidly. Industrial waste existed along watershed brings significant heavy metal pollutant that flows and difficult to control. Consequences of such activities were reflected in the degradation of reservoir environment indicated by sedimentation, water quality degradation and fish mass mortality because of up welling. The objectives of this research were: 1) to obtain data and information on heavy metal content in Cirata Reservoir waters, and 2) to observe and understand the effect of heavy metal to the fish cultured in floating net cage to support aquaculture in floating net cage system. Water quality and fish histology analyses were the methodologies used in this research. Descriptive and laboratory analysis were carry out to analysis the data. Based on observation and descriptive analyses, the content of heavy metal in Cirata Reservoir was classified as worse. Concentration of Pb, Cr, Hg, and Cd in the sample of water and tilapia digestive organ becomes fragile. Infiltration of hemoglobin cell, necrosis, degeneration and pigmentation occurred in body organs when up welling happened. Beside that, during up-welling digestive organ become easily breakable resulted in fish mass mortality in floating net cage. The affinity of hemoglobin to the toxic gas was higher than to oxygen, therefore aeration was not affective and mass mortality cannot be avoided. This was because of various events that occured to the fish and the other water biota that encompassed regular diffusion, bio-magnification, and bio-concentration to fish.
SISTEM INFORMASI GEOGRAFIS UNTUK APLIKASI MANAJEMEN TERPADU WILAYAH PESISIR DAN LAUT Saputra, Adang; Radiarta, I Nyoman; Prihadi, Tri Heru
Media Akuakultur Vol 1, No 1 (2006): (April 2006)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1924.582 KB) | DOI: 10.15578/ma.1.1.2006.41-47

Abstract

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DISTRIBUSI NITROGEN DAN FOSFOR PADA BUDIDAYA IKAN GABUS (Channa striata) DENGAN APLIKASI ECENG GONDOK (Eichhornia crassipes) DAN PROBIOTIK Saputra, Adang; Setijaningsih, Lies; Yosmaniar, Yosmaniar; Prihadi, Tri Heru
Jurnal Riset Akuakultur Vol 12, No 4 (2017): (Desember 2017)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (484.489 KB) | DOI: 10.15578/jra.12.4.2017.379-388

Abstract

Permasalahan yang dihadapi pembudidaya ikan dengan sistem intensif adalah meningkatnya limbah yang terakumulasi pada air dan sedimen. Limbah budidaya ikan pada umumnya berupa padatan dan nutrien terlarut pada air terutama nitrogen dan fosfor. Tujuan penelitian ini adalah mengkaji distribusi nitrogen total dan fosfor total pada budidaya ikan gabus secara intensif yang diberi eceng gondok Eichhornia crassipes dan probiotik (Pseudomonas aeruginosa dan Achromobacter insuavis). Penelitian dirancang menggunakan rancangan acak lengkap dengan perlakuan pemberian kombinasi eceng gondok dan probiotik (A), pemberian eceng gondok (B), dan pemberian probiotik (C), masing-masing perlakuan diulang tiga kali. Benih ikan gabus yang digunakan berukuran panjang 14,74 ± 0,01 cm dan bobot 25,53 ± 0,09 g dengan padat tebar 175 ekor/kolam (50 ekor/m3). Selama 90 hari masa pemeliharaan, ikan gabus diberi pakan berupa pelet dengan kandungan protein sekitar 30%. Jumlah pemberian pakan 5% dari biomassa dengan frekuensi pemberian empat kali dalam sehari (pagi, siang, sore, dan malam). Hasil penelitian menunjukkan nitrogen dan fosfor pada budidaya ikan gabus terdistribusi pada eceng gondok, sedimen, air, dan ikan. Eceng gondok menyerap nitrogen dan fosfor paling tinggi (P<0,05) dibandingkan air, ikan, dan sedimen. Laju pertumbuhan spesifik bobot (4,37 ± 0,01%/hari) dan biomassa (1,88 ± 0,01 g) ikan gabus tertinggi dicapai pada pemberian kombinasi eceng gondok dan probiotik. Hasil ini dapat dijadikan landasan untuk pengelolaan limbah nitrogen dan fosfor pada budidaya ikan gabus secara intensif.One of the problems in intensive aquaculture system is the the accumulation of waste in the water and sediment. Aquaculture wastes are discharged into the water in form of solids and dissolved nutrients which mostly consisted of nitrogen and phosphorus. The purpose of this study was to study the dynamics of total nitrogen and phosphorus in an intensive aquaculture media supplied with water hyacinth and probiotics (Pseudomonas aeruginosa and Achromobacter insuavis). The study was designed using a completely randomized design with treatment combinations of water hyacinth with probiotic (A), water hyacinth (B), and probiotic (C). Each treatment consisted of three replications. The seeds of snakehead used had body length of 14.74 ± 0.01 cm and weight 25.53 ± 0.09 g, stocked in ponds with stocking density of 175 individuals/pond (50 individuals/m3). During 90 days of rearing, the fish were fed with pellet with protein content of 30%. The amount of feeding was 5% of the biomass with feeding frequency of four times a day (morning, afternoon, evening, and night). The results showed that the produced nitrogen and phosphorus in the snakehead cultivation were distributed to water hyacinth, sediment, water, and fish. Water hyacinth absorbed most of the nitrogen and phosphorus compared to water, fish, and sediment. Higher specific growth rate (4.37 ± 0.01%/day) and biomass (1.88 ± 0.01 g) of snakehead were achieved in combination of water hyacinth and probiotic treatment. These results can be used as a basis for the management of nitrogen and phosphorus wastes in an intensive fish farming.
POTENCY AND EFFICACY TEST OF A VACCINE IN ADDITION WITH ADJUVANT AGAINST KOI HERPESVIRUS IN KOI (Cyprinus carpio) Sholichah, Lili; Yuhana, Munti; Lusiastuti, Angela Mariana; Prihadi, Tri Heru
Indonesian Aquaculture Journal Vol 11, No 1 (2016): (June 2016)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (656.285 KB) | DOI: 10.15578/iaj.11.1.2016.41-47

Abstract

Koi Herpesvirus (KHV) is a malignant virus infecting the goldfish and koi in all stadia and cause mortality up to 95%. The purpose of this study was to determine the potency and efficacy of inactivated-vaccine in addition with adjuvant against KHV in koi fish. The viral propagation was done using a KF-1 cell line in 25 cm3 flask. The cultured virus was harvested on 12 days post inoculation, and then the harvested virus was inactivated with 0.1% formalin as inactivated-vaccine. Three hundred of test fish (10.38 ± 1.25 g) maintained in 126 L of plastic containers with aeration, and fed with pellets twice a day. After 14 days of adaptation, the fish were divided into five treatments (A= vaccine; B= vaccine + Complete Freund’s Adjuvant; C= vaccine + Incomplete Freund’s Adjuvant; K+= positive control, and K-= negative control) and each treatment has four replicates. Vaccine was given by injecting intramuscularly of 0.1 mL per fish. All fish were challenged by injecting intramuscularly of 0.1 mL of KHV virus with concentration of 104.58 TCID50/mL after 21 days post vaccination. The results showed that the B treatment had higher (P<0.05) values of hematocrit level, lysozyme activity, and titer of antibody compared with positive control. In addition, the survival of fish in B treatment also had the highest percentages and significantly different compared to other treatments (P<0.05). The conclusion of this research was the application of inactivated KHV vaccine in 0.1% formalin with the addition of Complete Freund’s Adjuvant through the injection dose 0.1 mL fish-1 in 104.58 TCID50/mL capable to enhance the immune responses and raised the optimal protection of KHV antibody in koi fish.
Kajian Dampak Lingkungan Global dari Kegiatan Keramba Jaring Apung melalui Life Cycle Assessment (LCA) Prihadi, Tri Heru; Erlania, Erlania; Astuti, Iswari Ratna
Jurnal Riset Akuakultur Vol 3, No 2 (2008): (Agustus 2008)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1900.967 KB) | DOI: 10.15578/jra.3.2.2008.263-273

Abstract

Perubahan iklim global yang berlangsung saat ini memberikan pengaruh pada berbagai bidang, termasuk perikanan yang menyebabkan terjadinya degradasi lingkungan perairan. Hal ini berdampak pada muncul dan menyebarnya berbagai penyakit ikan, menurunnya laju pertumbuhan organisme perairan, bahkan hingga menimbulkan kematian massal ikan. Namun hal ini belum sepenuhnya dapat diatasi oleh para ilmuwan tanah air, bahkan bisa dikatakan baru sebagian kecil saja. Penerapan Best Management Practice (BMP) dengan aplikasi Life Cycle Assessment (LCA) akan sangat berarti dalam upaya penerapan perikanan budidaya berkelanjutan, dengan model pengelolaan kuantitatif. Dalam hal ini metode LCA secara kuantitatif merupakan pertama kalinya dilakukan di Indonesia. Penelitian ini bertujuan untuk mengetahui dan mengevaluasi kuantitas dan kategori dampak lingkungan akibat kegiatan budidaya keramba jaring apung (KJA) melalui LCA. Hasil penelitian menunjukkan bahwa kegiatan budidaya di KJA menimbulkan dampak yang signifikan terhadap lingkungan perairan. Dari berbagai faktor yang berperan dalam kegiatan budidaya KJA, pakan ikan merupakan faktor yang paling dominan dalam menghasilkan dampak lingkungan global (di atas 70%), berupa pemanasan global, penurunan jumlah sumberdaya abiotik, eutrofikasi, penipisan lapisan ozon, toksisitas pada manusia, dan penurunan jumlah keanekaragaman hayati. Dari faktor pakan tersebut, unsur yang paling berpengaruh dalam menghasilkan dampak lingkungan adalah soybean Brazil dan winter wheat, sehingga perlu dicari alternatif bahan untuk mensubstitusi kedua unsur tersebut. Demikian juga faktor-faktor lainnya (seperti: polystyrene foams, drum plastik, bambu, jaring, besi, skala budidaya, dan lain-lain) mempunyai peranan terhadap dampak yang ditimbulkan terhadap lingkungan perairan.Global climate change has been affecting many sectors, including fisheries causing aquatic environment degradation such as fish disease outbreaks, decreasing growth rate of fish and other aquatic organisms, and further, may cause fish mass mortality. Recently these conditions have not fully solved by Indonesian scientists. Application of Best Management Practice (BMP) with Life Cycle Assessment (LCA) using quantitative model is the best way for implementing a Sustainable Aquaculture. This case was the first attempt of using the quantitative LCA in Indonesia. This research was conducted to quantify and evaluate environmental aspects that were affected by floating net cage (KJA). The result showed that fish culture in KJA contributed significant impacts to the aquatic environment. From many factors of KJA, feed was the most dominant factor that caused the global environmental impact (above 70%), in form of global warming potential, abiotic depletion, eutrophycation, ozone depletion potential, human toxicity, and biodiversity depletion. Feed consist of many substances. Soybean Brazil and winter wheat were the major substances that brought out the most environmental impact. Thus, it is important to find alternative materials to substitute these substances. Other factors of KJA (plastic drums, polystyrene foam, bamboo, steel, net, farming scale, etc.) also affected the quantity and kind of impacts to aquatic environment.
KARAKTERISASI FENOTIPE DAN GENOTIPE TIGA POPULASI IKAN TENGADAK (Barbonymus schwanenfeldii) Kusmini, Irin Iriana; Gustiano, Rudhy; Radona, Deni; Prakoso, Vitas Atmadi; Putri, Fera Permata; Prihadi, Tri Heru
Jurnal Riset Akuakultur Vol 11, No 3 (2016): (September 2016)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.256 KB) | DOI: 10.15578/jra.11.3.2016.207-216

Abstract

Ikan tengadak, Barbonymus schwanenfeldii merupakan salah satu jenis ikan lokal yang potensial untuk dikembangkan. Kemungkinan keberhasilan pengembangan ikan ini sangat tinggi karena ikan tengadak sekerabat dengan ikan tawes. Studi keragaman genetik dilakukan dalam upaya pemanfaatan sumber daya genetik untuk kegiatan budidaya secara berkelanjutan. Tujuan penelitian ini adalah untuk menganalisis keragaman dan kekerabatan antara populasi ikan tengadak asal Jawa, Sumatera, dan Kalimantan. Analisis fenotipe dilakukan berdasarkan pengukuran truss-morfometrik, sedangkan analisis genotipe dilakukan secara molekuler menggunakan metode PCR-RAPD dengan primer OPA-08, OPA-09, dan OPC-02. Hasil analisis fungsi kanonikal truss-morfometrik menunjukkan sebaran pengukuran ketiga populasi berada pada kuadran yang berbeda dengan persentase indeks keseragaman tertinggi pada populasi Sumatera dan Jawa (100%). Analisis genotipe menunjukkan ikan tengadak populasi Kalimantan memiliki nilai polimorfisme (50,00) dan heterozigositas tertinggi (0,206); secara kekerabatan ikan tengadak Kalimantan memiliki jarak yang jauh dengan ikan tengadak Jawa dan Sumatera.Tinfoil barb Barbonymus schwanenfeldii is one of potential local fish species for aquaculture development. The successful probability of aquaculture development of tinfoil barb is very high due to close genetic relationship with silver barb. Study related to genetic diversity was conducted in order to explore genetic resources for sustainable aquaculture. The aim of this study was to analyze the genetic diversity and relationship between tinfoil barb populations from Java, Sumatra, and Kalimantan. Phenotype analysis was conducted by truss morphometric, whereas genotype analysis conducted by PCR-RAPD using OPA-08, OPA-09, and OPC-02 primers. The results of canonical correlation analysis from truss-morphometric measurements showed that the distribution of three populations were located in different quadrants with the highest similarity index percentage on Sumatra and Java population (100%). Genotypic analysis showed that tinfoil barb population from Kalimantan revealed the highest value of polymorphism (50.00) and heterozygosity (0.206), Kalimantan population have distantly genetic relationship to tinfoil barb from Java and Sumatra.