Articles

THE EFFECTIVENESS OF Acanthus Ilicifolius IN PROTECTING TIGER PRAWN (Penaeus monodon F.) FROM Vibrio harveyi INFECTION Saptiani, Gina; Prayitno, Slamet Budi; Anggoro, Sutrisno
JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 2 (2012): Volume 15, Number 2, Year 2012
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Sea holly (Achantus ilicifolius) is a mangrove plant that often used by coastal society as a traditional medicine.  It is potential to be developed as the sources of pharmaceutical products. This study aims at assessing the effectiveness of sea holly leaves as antibacterial agent and as an agent to enhance the durability of shrimp against Vibrio harveyi.  First, dried sea holly leaves were extracted with methanol, after that, it was fractionated with silica gel column method using solvent n-hexane, ethyl acetate, and n-butanol.  The treatments given to tiger prawn were crude extract, the fraction of ethyl acetate, and n-butanol, and it was given by immersion.   Next, the challenge test was conducted toward Vibrio harveyi.  As the result, the extract and the leaves of A. ilicifolius possess activities of inhibiting the growth of V. harveyi in vivo, reducing the prevalence of attacks and improving survival of prawn. In general, based on clinical symptoms and pathological anatomy, n-butanol fraction of the A.  ilicifolius leaves possess the best protection, along with ethyl acetate fraction and the crude.
APPLICATION OF REPETITIVE SEQUENCE-BASED PCR ON THE RICHNESS OF VIBRIO ON THE TIGER SHRIMP (Penaeus monodon Fab.) arjito, S; Ningrum, Nestin E.W.; Radjasa, Ocky Karna; Prayitno, Slamet Budi
JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 3 (2012): Volume 15, Number 3, Year 2012
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A molecular-based study was conducted to estimate the richness of the Vibrio on  tiger shrimp (Penaeus monodon Fab.) from brackish water pond of Mororejo, Kendal. Tiger shrimps were collected from the extensive brackish water pond and a total of 22 isolates were obtained from hepatopancreas and telson of tiger shrimp. Based on the repetitive sequence-based polymerase chain reaction (rep-PCR), it was found that two groups of Vibrio. To investige the efectiveness of rep-PCR in estimating the richness of Vibrio on tiger shrimps, three isolates  (JTW 01, JTW 03 and JTW 06) were chosen for further investigation. On the basis of sequence analysis, the result showed  that the JTW 01, JTW 03 and JTW 06 were closely related to Vibrio sp. Absa7 clone 423.1, Vibrio splendidus and Vibrio splendidus, respectively.  The result proved that two assosiated of Vibrio on tiger shrimp were Vibrio sp. Absa7 clone 423.1 and Vibrio splendidus. Therefore  the present study highlights the effectiveness of rep-PCR in rapid grouping and estimating the richness of Vibrio on  tiger shrimp.
THE GROWTH ANALYSIS OF Stichopus vastus (Echinodermata: Stichopodidae) IN KARIMUNJAWA WATERS Sulardiono, Bambang; Prayitno, Slamet Budi; Hendrarto, Ign Boedi
JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 3 (2012): Volume 15, Number 3, Year 2012
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Stichopus vastus is one of sea cucumber resources utilize which has commercial economic value, locally known  as “gametes”. Because this species is continuously exploited, while the nature of life has not been known, one of them especially its growth.  Aspect of growth is an important parameter which is used as a basis for evaluating these resources, hence this resource is well managed and can be utilized in a sustainable manner. The study aims to: (1) estimates the growth characteristics mortality rate, recruitment and potential use of sea cucumber of  S.  vastus in Karimunjawa. The study is expected to give beneficial to the species of sea cucumber resource management strategy at  Karimunjawa waters, Jepara. The analysis of the growth parameters of S. vastus obtained values ​​of growth coeficient (K) of 0.55 year-1 and length infinity L ∞ value of 315.80 mm. Therefore the obtained values ​​of K tend to be close to zero value, it indicates the nature of growth tends to slow and with a relatively long life.  Life span of  S. vastus is 5.41 years, which can achieve body lengths of 283.06 to 296.91 mm, and the monthly growth rate ranged from 9.0 to 12.37 mm. The results of the analysis of mortality showed that S. vastus has value of total mortality (Z) of 0.98,  natural mortality (M) of 0.298, and catch mortality (F) of 0.682, with the rate of exploitation (E) of 0.6963. This suggests that the rate of exploitation is above the threshold standard set by the Government of 0.5 (BRKP, 2004). The analysis of the S. vastus the recruitment showed that the higest percentage recruit occurred in May-June is 17.16 - 18.33%. This is presumably due to a population increase of the spawning process in those months, although the value of the addition is not very significant.   It is suggested the need for regulations regarding: (a) the catchment season which is based on the tendency of the reproductive patterns of S. vastus, in Karimujawa, (b) restrictions on the size of the catch, (c) catch quotas, (d) the methods that are environmentally friendly catch, (e) permits the ship / boat used to catch sea cucumbers business. To support the success of regulation, it is necessary to strengthen community institutions through the management of sea cucumber resource-based society, with the Co-management approach.
DETERMINATION OF MERCURY’S BIOACCUMULATION FACTOR IN MILK FISH (Chanos chanos) OF SEMARANG MUNICIPALITY FISHPONDS USING NEUTRON ACTIVATION ANALYSIS Prayitno, Slamet Budi
JOURNAL OF COASTAL DEVELOPMENT Vol 3, No 3 (2000): Volume 3 Number 3 Year 2000
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Determination of bioaccumulation factor of mercury (200Hg) in chanos chanos of Semarang municipality fishponds has been carried out by applying the neutron activation analysis (NAA) and gamma spectrometry analysis. The gamma spectrometry technique was employed to analyze the gamma radiation exposure from activated samples. The heavy metal 200 Hg can be identified in water and milk fish (Chanos chanos), along with natural radionuclides 40K in water. The concentration of Mercury in the water at the 3 villages was 0.950 10-6 ppm, 284 10-6, and 350 10-6 ppm respectively. Whilst mercury concentration in the milk fish was 186 10-3, 068 10-3, and 098 10-3 ppm for Mangunharjo, Mangkang, dan Karanganyar respectively. Bioaccumulation factor can be estimated from the ratio of these heavy metal element in the sample and the water. Result shows that the bioaccumulation factor for mercury at 3 villages (Mangunharjo, Mangkang dan Karanganyar) is 195.942, 238.961, 279.614 respectively.
THE EFFECT OF DIFFERENT C:N AND C:P RATIO OF MEDIA ON THE CONTENT OF POLYHYDROXYBUTYRATE IN BIOFLOC INOCULATED WITH BACTERIUM Bacillus cereus ., Supono; Hutabarat, Johannes; Prayitno, Slamet Budi; Darmanto, YS
JOURNAL OF COASTAL DEVELOPMENT Vol 16, No 2 (2013): VOLUME 16, NUMBER 2, YEAR 2013
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Biofloc technology  has  added values in aquaculture management, both in water quality  management and feeding  management.  As an optional feed, biofloc is capable to enhance growth due to high protein content.  Bacteria, main component biofloc, can produce polyhydroxybutyrate (PHB) as reserve of energy and growth accelerator for fish. The aim of the research were to study the effect of the different C:N and C:P ratio of media on the content of polyhydroxybutyrate in biofloc and to determine optimum media to produce high polyhydroxybutyrate content in biofloc. The experiment was arranged in factorial  with completely randomized design  in  three replications.  Treatments were C:N ratio of 15, 20, 25 and C:P ratio of 75, 100, and 125. The result showed that C:N ratio and C:P ratio of media and their interaction  affect the content of polyhydroxybutyrate in biofloc.  C:N ratio of 20 and C:P ratio of 125 resulted in most polyhydroxybutyrate (29.25±7.376 mg g-1 biofloc dry weight). Ratio of  C:N  of media gave linier and quadratic responses and C:P ratio of media gave linier one. Optimum polyhydroxybutyrate production was obtained at C:N ratio of 20.9 and C:P ratio of  125 resulting in 29.66 mg  g-1 biofloc dry weight (2,97%)
THE PERFORMANCE OF BI-FILTER ON THE PRODUCTION AND DISEASE INCIDENCE OF PRAWN (PENAEUS MONODON FAB.) Prayitno, Slamet Budi
JOURNAL OF COASTAL DEVELOPMENT Vol 4, No 1 (2000): Volume 4, Number 1, 2000
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It has been long known that the most potent factor that influences prawn production was unfavorable environment due to industrial, agriculture and habitat wastes as well as improper application of good husbandry management. This research was designed to study the effect of biological filter on the growth of tiger prawn, water qualilv, and disease incidence. 7`wo sets of experiments, namely field stddy and laboratory experiment were conducted . Field survey was carrted out to collect field data on actual husbandry practices, production, water and soil quality. Laboratory experiment was aimed at evaluating the efhctiveness of biologicalfilter and disease incidence. Juvenile prawn e (j 5 gr.) at a density of 15 animals/M2; 20 animals/M2, and 25 animals/M2 were used as experimental animals. The observed parameters were field productivity, growth, survival rate, water quolitv, bacterial counts, soil quality and disease incidence. Experiments were carried in 3 replicates. Field survey showed that prawn production decreased steephr at the third production cycle as low as 627.65 kg/Ha compared to the first cycle (1893. 28 kg/lla}. Most prawn were weak, and very vulnerable to diseases. Bacterial count was more than I0Jcfii/ml. Water quality rapidly declined as prawn grew especially during the second and third cycles. Laboratory experiment with biologicalfilter in contrast demonstrated better water quality and healthier prawn,. although water quality declined slightly along with both the age and density. The growth rate was better than during field trials; among three treatments there were signdicant dyferences; the survival rate was best at density of I 5 animals/M2 but, the best estimated production was reached by density of 20 animals/M2 producing 2645 kg/Ha/cycle without any problems for around 4 cycles. Disease incidence and parasites infestation was undetected in the tank during the experiment.
PENGARUH PEMBERIAN EKSTRAK DAUN JERUJU (Acanthus ilicifolius) TERHADAP PROFIL DARAH IKAN KERAPU MACAN (Epinephelus fuscoguttatus) Agung, Lukman Anugrah; Prayitno, Slamet Budi; -, Sarjito
Journal of Aquaculture Management and Technology Vol 2, No 1 (2013) : Journal of Aquaculture Management and Technology
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ABSTRAK Ikan kerapu termasuk salah satu komoditas perikanan laut bernilai ekonomis penting. Ikan kerapu macan memiliki beberapa keunggulan diantaranya nilai gizi yang tinggi dan pertumbuhan yang lebih cepat daripada ikan kerapu jenis lain. Salah satu permasalahan dalam budidaya ikan kerapu macan adalah serangan penyakit. Salah satu cara untuk mencegah terjadinya penyakit ialah meningkatkan imunitas ikan. Daun jeruju (Acanthus ilicifolius) merupakan bahan herbal yang diduga mampu meningkatkan imunitas ikan kerapu macan. Peningkatan imunitas ikan dapat diketahui dengan memeriksa profil darahnya. Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian ekstrak daun jeruju (Acanthus ilicifolius) terhadap profil darah ikan kerapu macan meliputi jumlah eritrosit, jumlah leukosit, diferensial leukosit dan aktivitas fagositosis. Rancangan percobaan menggunakan Rancangan  Acak Lengkap  (RAL)  dengan  4  perlakuan  dan  3  ulangan. Dosis ekstrak daun jeruju yang digunakan adalah: perlakuan A (0 gr/kg); perlakuan B (5 gr/kg); perlakuan C (10 gr/kg); dan perlakuan D  (15  gr/kg). Pemberian ekstrak daun jeruju melalui pakan dilakukan selama 14 hari. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun jeruju berpengaruh nyata terhadap jumlah eritrosit (P<0,05) dan berpengaruh sangat nyata terhadap jumlah leukosit (P<0,01) namun tidak berpengaruh nyata terhadap diferensial leukosit dan aktivitas fagositosis ikan kerapu macan (P>0,05). ABSTRACT Grouper is one of economically commodity fish. Tiger grouper has several advantages including high nutritional value and rapid growth than other types of grouper. One of the problems in the tiger grouper culture  is a disease. One way to prevent the disease is to improve the immunity of fish, Jeruju leaf extracts is one of the herbal material which can increased fish imunity. Increased of fish immunity can be detected by examining of blood profile. The purpose of this experiment is determine the effect of jeruju leaf extract  (Acanthus ilicifolius) on the blood profile of tiger grouper i.e. : number of erythrocyte, leukocyte, differential leukocyte, and phagocyty activity. The design of the experiment is a completely randomized design (CRD) with 4 treatments and 3 replications. Jeruju (acanthus ilicifolius) leaf extracts were mixed with the artificial feed at concentrations of 0 g/kg ,5 g/kg, 10g/kg and 15 g/kg and  had been fed to grouper for 14 days period. The results showed that administration of jeruju leaf extract significantly effect on the number of erythrocytes (P<0.05) and highly significant effect on  the number of leukocytes (P <0.01) but no significant effect (P>0,05) on differential leukocyte, and phagocyty activity of tiger grouper.
Potensi Chlorella sp. sebagai Imunostimulan untuk Pencegahan Penyakit Bercak Putih (White Spot Syndrome Virus) pada Udang Windu (Penaeus Monodon) Ermantianingrum, Aulia Ayu; Sari, Rohita; Prayitno, Slamet Budi
Journal of Aquaculture Management and Technology Vol 1, No 1 (2012): Journal Of Aquaculture Management and Technology
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ABSTRAK Chlorella sp. merupakan salah satu mikroalga yang berpotensi sebagai imunostimulan untuk meningkatkan sistem pertahanan tubuh udang windu terhadap infeksi penyakit. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian Chlorella sp. terhadap sistem pertahanan tubuh udang windu. Metode yang digunakan adalah metode eksperimental menggunakan rancangan acak lengkap (RAL) dengan 4 perlakuan dan masing-masing terdiri dari 3 ulangan. Perlakuan yang diujikan yaitu penambahan Chlorella sp. pada pakan dengan perlakuan A (tanpa penambahan Chlorella sp.), perlakuan B (5 gr/kg pakan),  perlakuan C (10 gr/kg pakan), dan perlakuan D (15 gr/kg pakan). Hewan uji yang digunakan adalah udang windu (P. monodon) stadia juvenil dengan bobot rata-rata 5,37±0,3 gram. Parameter yang diamati yaitu total haemocyte count (THC), differential haemocyte count (DHC), dan kelulushidupan udang windu yang diinfeksi WSSV. Hasil penelitian menunjukkan bahwa pemberian Chlorella sp. tidak berpengaruh nyata (P>0,05) terhadap total haemocyte count (THC), differential haemocyte count (DHC), dan kelulushidupan udang windu yang diinfeksi WSSV. Pencegahan penyakit pada udang windu dapat dilakukan dengan pemberian imunostimulan untuk meningkatkan sistem imun, meskipun tidak ada udang windu yang hidup dalam 6 hari setelah diinfeksi WSSV. Kualitas air selama penelitian masih dalam kisaran layak untuk kehidupan udang windu.   ABSTRACT Chlorella sp. is a microalga that has potency as an immunostimulant to increase immune system of black tiger shrimp. The purpose of this research was to find out the effect of Chlorella sp. to increase immune system of black tiger shrimp. The experiment method used in this research was Completely Randomized Design (RAL) with 4 treatments and each of them consists of 3 replication. The treatments were A without addition of Chlorella sp., treatment B, C, and D with addition of Chlorella sp. 5, 10, 15 gram/kg diet respectively. The experiment animals was black tiger shrimps (P. monodon) juvenile with average weight 5,37±0,3 gram. Parameters observed were total haemocyte count (THC), differential haemocyte count (DHC), and survival rate of black tiger shrimp infected by WSSV. The results of research indicated that the addition of Chlorella sp. Within the diets showed no significant effect (P>0,05) on total haemocyte count (THC), differential haemocyte count (DHC), and survival rate of black tiger shrimp. Disease prevention of black tiger shrimp could be done by an administration of immunostimulant to stimulate or increase immune system. However, there was no shrimp was survived within 6 days after challenged with WSSV. The water quality during the research within ideal range for the life of black tiger shrimps.  
Pengaruh Ekstrak Daun Jambu Biji (Psidium guajava) Untuk Menginaktifkan Viral Nervous Necrosis (VNN) Pada Ikan Kerapu Bebek (Epinephelus fuscoguttatus) Amelia, Nita; Prayitno, Slamet Budi
Journal of Aquaculture Management and Technology Vol 1, No 1 (2012): Journal Of Aquaculture Management and Technology
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Permasalahan yang sering dihadapi pembudidaya ikan kerapu adalah infeksi Viral Nervous Necrosis (VNN) yang dapat menyebabkan kematian 100%, terutama pada stadia larva. Antibiotik dalam penanggulangan penyakit VNN menyebabkan residu dan resistensi pada ikan, sehingga perlu alternatif herbal salah satunya dengan penggunaan ekstrak daun jambu biji yang mengandung flavonoid. Flavonoid adalah senyawa bioaktif yang akan mengubah reaksi tubuh terhadap senyawa lain sehingga dapat bersifat sebagai antivirus. Tujuan penelitian ini untuk mengetahui efektifitas penggunaan ekstrak daun jambu biji dalam menginaktifkan VNN dengan melihat 1. Hasil PCR ikan kerapu sebelum dan setelah diinjeksikan VNN dan ekstrak daun jambu biji. 2. Gejala klinis ikan kerapu yang terinfeksi VNN. 3. Histopatologi organ yang terinfeksi VNN. 4. Rata-rata waktu kematian ikan kerapu. 5. Tingkat mortalitas dan kelulushidupan ikan kerapu. 6. Kelayakan kualitas air untuk budidaya ikan kerapu. Metode yang digunakan eksperimental, disain Rancangan Acak Kelompok. Delapan puluh ekor ikan kerapu dibagi dalam 4 kelompok, masing-masing kelompok berisi 4 keranjang basket dan setiap keranjang basket berisi 5 ekor ikan. Ikan kontrol hanya diinjeksi VNN 0,1 ml/ekor. Perlakuan 1 ikan diinjeksi virus 0,1 ml/ekor dan ekstrak daun jambu biji 0,1 ml/ekor dengan konsentrasi 1000 µg/ml. Perlakuan 2 ikan diinjeksi virus 0,1 ml/ ekor dan ekstrak daun jambu biji 0,1 ml/ ekor dengan konsentrasi 100 µg/ml. Perlakuan 3 ikan diinjeksi virus 0,1 ml/ ekor dan ekstrak daun jambu biji 0,1 ml/ ekor dengan 10 µg/ml. Pengamatan deskriptif meliputi, hasil PCR, gejala klinis, histopatologi dan kualitas air. Perhitungan data meliputi, rata-rata waktu kematian, mortalitas dan kelulushidupan ikan kerapu. Hasil PCR sebelum penginjeksian, negatif ikan kerapu tidak terinfeksi VNN dan setelah diinjeksi VNN dan ekstrak daun jambu biji hasil PCR positif terinfeksi VNN. Gejala klinis ikan kerapu yang terinfeksi VNN adalah berenang memutar, berdiam diri di dasar, warna tubuh lebih gelap. Histopatologi menunjukkan adanya inclusi body (sel mengalami nekrosis), hipertropy (sel mengalami pembesaran) dan vacuola (ruang-ruang kosong). Rata-rata waktu kematian ikan kontrol 132,7 jam, perlakuan 1 87,5 jam, perlakuan 2 114,8 dan perlakuan 3 98,1 jam. Tingkat mortalitas pada ikan kontrol 85%, perlakuan 1 80%, perlakuan 2 85% dan perlakuan 3 75%. Kelulushidupan pada ikan kontrol 15%, perlakuan 1 20%, perlakuan 2 15% dan perlakuan 3 25%. Kualitas air untuk budidaya dapat dikatakan layak untuk budidaya. Hasil PCR, histopatologi, rata-rata waktu kematian, mortalitas dan kelangsungan hidup menunjukan bahwa ekstrak daun jambu biji tidak efektif karena konsentrasi, dosis dan metode ekstrak daun jambu biji tidak tepat untuk menginaktifkan VNN.
PENGARUH PENGGUNAAN EKSTRAK DAUN JERUJU (Acanthus ilicifolius) TERHADAP KELULUSHIDUPAN IKAN KERAPU MACAN (Epinephelus fuscogutattus) YANG DIINFEKSI Vibrio alginolyticus Aonullah, Asep Akmal; Prayitno, Slamet Budi; -, Sarjito
Journal of Aquaculture Management and Technology Vol 2, No 1 (2013) : Journal of Aquaculture Management and Technology
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ABSTRAK Kendala utama budidaya ikan kerapu adalah tingginya tingkat kematian terutama pada benih sebagai akibat serangan bakteri dan patogen. Penelitian ini bertujuan untuk mengkaji pengaruh penggunaan ekstrak daun jeruju (Acanthus ilicifolius) terhadap kelangsungan hidup ikan kerapu macan  (Epinephelus fuscogutattus) yang diinfeksi Vibrio alginolyticus. Ikan kerapu macan (E. fuscogutattus) sebanyak 120 ekor diberikan perlakuan selama 40 hari dengan pemberian pakan yang ditambahkan ekstrak daun jeruju (A. ilicifolius). Uji tantang dilakukan dengan menyuntikan suspensi bakteri V. alginolyticus secara intramuskular. Prosedur pengamatan dilakukan selama 10 hari pascainfeksi meliputi gejala klinis, kelulushidupan serta gambaran darah ikan kerapu macan. Hasil penelitian menunjukkan gejala klinis ikan sakit diantaranya bergerak lamban, berenang tidak teratur (erratic swimming), berenang di permukaan, warna tubuh ikan menjadi gelap, timbulnya luka di bagian punggung serta geripis pada bagian sirip ikan dan pembengkakan pada bola mata (exopthalmia). Pemberian ekstrak daun A. ilicifolius pada pakan menunjukkan hasil tidak berpengaruh nyata (P>0,05) terhadap kelulushidupan dan jumlah total eritrosit ikan kerapu macan pascainfeksi, akan tetapi penambahan ekstrak daun A. ilicifolius menunjukkan hasil berpengaruh nyata (P<0,05) pada hari ke-8 dengan nilai tertinggi 9,39x104 sel/mm3 serta berpengaruh sangat nyata (P<0,01) terhadap aktifitas fagositik hari ke-4 dengan nilai tertinggi 65%. Dosis terbaik dalam penelitian ini adalah penambahan ekstrak daun A. ilicifolius sebanyak 5 gr/kg pakan. ABSTRACT This research was aimed to find out the effect of using leaf extract Acanthus ilicifolius toward survival rate of tiger grouper (Epinephelus  fuscogutattus) infected by Vibrio alginolyticus. 120 fish E. fuscogutattus was treated 40 days with the added leaf extract A. ilicifolius in the feeding process. The test was done by injecting of bacterial suspensions V. alginolyticus in the intra-muscular of the fish. Observation procedure was performed for 10 days after the infection such as clinical symptoms, survival rate and blood picture of the fish. The results showed clinical symptoms of sick fishes i.e. abnormally swiming activity, erratic swimming, swimming on the surface, dark colouration of the skin, a wound on the back and fins, and exopthalmia. The test results showed that extract of A. ilicifolius leaf not significantly different (P>0.05) on survival rate and total erythrocyte of E. fuscogutattus post-infection, but the addition of leaf extracts A. ilicifolius indicate results significantly different (P<0.05) on day 8 with the highest score of 9.39 x104 cells/mm3 and differed significantly (P<0.01) on  phagocytic activity day 4 with a highest score of 65%. The best dosage in this research was 5 gr/kg of feed.
Co-Authors - Aminah - Istikhanah - Sarjito - Susanti, - - Triyaningsih Adhi Kurniawan Agatya Sara Ardiantami, Agatya Sara Agil Setya Utomo, Agil Setya Agung Sudaryono Alfabetian Harjuno Condro Haditomo Alfabetian Herjuno Condro Haditomo Amanda Mega Putri, Amanda Mega Ambaryanto Ambaryanto ANGELA MARIANA LUSIASTUTI Anggun Putriani Situmorang, Anggun Putriani Anisa Dwiaryani Latifah Aprilia Dwi Indriani Ardulanisa, Ridha Asep Akmal Aonullah Aulia Ayu Ermantianingrum Aulia Resty Wijayanti Awanis, Annisa Amalia Ayi Santika Ayu Wulandari Azis Nur Bambang Bambang Sulardiono Choeronawati, Anggih Isti Chyntia Arindita Dani Indrarini Devitha Tri Utami Dian Ratna Sari Edward Raharja, Edward Endah Setyowati Eni Ashfa Ashofa Ferdian Bagus Feriandika Gina Saptiani Haeruddin, . Hardyta Noviar Rahma Heraedi, Arifianto Ign Boedi Hendrarto Indah Febry Hastari Intan Eska Amalia Syahida Johannes Hutabarat Kurniawan Kurniawan Linuwih Aluh Prastiti, Linuwih Aluh Lukman Anugrah Agung Lusi Dianti Marwenni Siregar, Marwenni Mohammad Saleh Lubis Nailil Muna Nestin E.W. Ningrum Nita Amelia Nur Annisa Nuri Nia Yanti, Nuri Nia Nurul Hidayati OCKY KARNA RADJASA Pramudita Apriliyanti Pricilia, Shella Pungki Nanda Pratama R. Dewi Dharina Nurjannah Restiana Wisnu Ariyati Rohita Sari Rusydina Qamarul Salikin S arjito Sahala Hutabarat Sarah, Humidah Sarjito - Sarjito Sarjito Sekar Ayu Chairunnisa Siti Nurjanah Siti Ziyadaturrohmah Sri Hastuti Sri Nuryati Supono . Sutrisno Anggoro Sutrisno, Bagus Oktori Teguh Eko Suryo Agil Hermawan Tristiana Yuniarti Utari, Viola Indah Vivi Endar Herawati Yelliana Fatmawati Suwarno YS Darmanto Zairin Zain