Adi Prayitno
Medical School of Sebelas Maret University, Surakarta

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Ekspresi interleukin-2 dan interleukin-10 pada kanker serviks uteri dengan positif HPV Prayitno, Adi; Darmawan, Ruben; Yuliadi, Istar
Jurnal Kedokteran Yarsi Vol 12, No 1 (2004): Jurnal Kedokteran Yarsi
Publisher : Jurnal Kedokteran Yarsi

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ABSTRACTNow the pathogenesis of cervical cancer is pointed to lzuman papilloma virus (HPV). The immunity against cancer many unknown. Interleukin-2 (IL-2) act as growth and differentiation factor for natural killer (NK) cells. Interleukine-10 (IL-10) is a unique because it can be press and stimulate the immune response. The objective of this experiment is to known the expression of IL-2 and IL-10 in cervical cancer with HPV positive. DNA was isolated from nineteen samplescervical cancer tissues frozen section. Diagnose related with HPV made by PCR method. Paraffin block of the tissues cervical cancer with HPV infection was cut in throughly cleaned cryotome and place in glass plate that covered with polyelysine.The imnzunohistocheinistry is done with monoclonal antibody anti IL-2 and IL-10 with TSA -indirect method. Seventeen samples diagnosed HPV positive. The Result of this experiment show that expression of IL-2 and IL-10 in cervicalcancer with HPV positive are in mild category (30-70%). The experiment that related with cervical cancer immunology is very suggested.KEYWORDS IL-2, IL-10, Cervical cancer, HPV
Profil IL-2 pada serum penderita kanker nasofaring yang terinfeksi virus Epstein-Barr Budian, Nendyah Ratna; Haryana, Sofia Mubarika; Sosroseno, Wihaskoro; Prayitno, Adi
Jurnal Kedokteran Yarsi Vol 10, No 2 (2002): Jurnal Kedokteran Yarsi
Publisher : Jurnal Kedokteran Yarsi

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ABSTRACTInterleukin 2 (IL-2) known as a T cell Growth Factor. IL-2 production is a key for the production of other cytokine by T-cell as an immune response against virus. The aim of thisstudy is to know the profile of IL-2 serum in nasophaynx carcinoma (NPC) patient with Epstein-Barr virus infection. Thirty two sample with NPC, five with Mononucleosis lnfectiosa (MI) and 10 as control are collected. The concentration of IL-2 is confirmed by ELISA method. The serological test to catch the Epstein-Ban virus is done by applying the IgA anti VCA and IgA anti ABNA test. The Statistical test shows that the highestconcentration of lL-2 seems to be found in NPC, compared with that found in MI, while the lowest concentration of that substance is found in the control. The study on IL-2 in serumof patlent infected with EBV is strongly suggested.KEYWORDS IL-2, serum, carcinoma nasopha ynx, EBV
Incidence of HPV Infection in Oral Squamous Cell Carcinoma and Its Association with the Presence of p53 & c-myc Mutation : A Case Control Study in Muwardi Hospital Surakarta Prayitno, Adi; Rukmo, Mandojo; Mudigdo, Ambar; Dalono, Dalono; Asnar, Elyana; Rahayu, Retno Puji; Purwanto, Joko Agus; Puspaningsih, Ni Nyoman Tri; Kuntoro, Kuntoro; Harjanto, Harjanto; Putra, Suhartono Taat
Journal of Dentistry Indonesia Vol 17, No 2 (2010): August
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (487.301 KB) | DOI: 10.14693/jdi.v17i2.44

Abstract

Introduction: Annual incidence rates for oral and pharyngeal cancer are estimated at 25 cases per 100,000 in developing countries. Human papilloma virus (HPV) was implicated in pathogenesis of Cancer. The mutations of p53 and c-myc are found 50% in cancer. Objective: Aims of this research were to know the incidence of OSSC patient which realized HPV infection without p53 and c-myc gene mutation. Materials and Methods: Tissue biopsy frozen sections were taken from BOSC (Benign Oral Squamous Cell) and OSCC (Oral Squamous Cell Carcinoma) patients collected from Oral and Dental Departement of dr Muwardi Distric Hospital in Surakarta from January 2007 to January 2008. To amplify L1-HPV gene for fixed the HPV stressor. To amplify p53 and c-myc genes, continued with SSCP (Single Strand Conformational Polymorphisme) analysis and followed with measurement using densitometer, to see mutation existence. The collected data were analyzed with Chi Square. Results: BOSC patient identified 23% with HPV infections and OSCC patient identified 73% with HPV infections. Hundred percent BOSC patient with HPV infection without mutation in p53 gene and c-myc gene, 81% OSCC patient with HPV infection without mutation in p53 gene and 91% OSCC patient with HPV infection without mutation in c-myc gene. Chi  square analysis showed significant difference between BOSC and OSCC patients with HPV infection without mutation in p53 and c-myc gene. Conclusion: HPV is a factor for pathogenesis of OSCC.DOI: 10.14693/jdi.v17i2.44
The Role of Heat Shock Proteins in Pathogenesis of Oral Squamous Cell Carcinoma Prayitno, Adi; Rukmo, Mandojo; Mudigdo, Ambar; Dalono, J. B.; Asnar, Elyana; Rahayu, Retno Puji; Purwanto, Joko Agus; Puspaningsih, Ni Nyoman Tri; Kuntoro, Kuntoro; Harjanto, Harjanto J. M.; Putra, Suhartono Taat
Journal of Dentistry Indonesia Vol 16, No 2 (2009): August
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2754.76 KB) | DOI: 10.14693/jdi.v16i2.91

Abstract

Cell in the distress situation, denaturation of proteins may occur, and may also respond by expressing stress proteins. However, such homeostasis effort does not always succeed and even may lead to disease, including cancer. In distress situation also ensue much protein misfolding. Objective: This research were to explain the role of heat shock protein 40 (Hsp40) and Hsp70 in pathogenesis of occurred oral squamous cell carcinoma (OSCC) patient which realized human papilloma virus (HPV) infection. Material and Method: Tissue biopsy frozen section were taken from BOSC and OSCC patients was cut into three part. Parrafin blocks were made from cutting I, which was subsequently stains with HE to ascertain the type of neoplasm. Cutting II was subjected to DNA isolation. The DNA isolation results were subjected to PCR to amplify L1-HPV gene for fixed the HPV stressoor. Protein isolation was treated from Cutting III, folloewd with Blottdot test by using antibody monoclonal anti Hsp40 and Hsp70 and continued with measurement using densitometer to find the concentration of Hsp40 and Hsp70. The collected data were analyzed with F Test (Manova) and discriminant analysis. Result: This experiment showed the differences in concentration of Hsp40 (p<=0,070) and Hsp70 (p<=0,006) between beningn oral squamous cell (BOSC) and OSCC patients which realized HPV infection. Conclusion: This experiment proved that OSCC patients which realized HPV infection indicated an up regulated of Hsp70 concentration, so that there was occurs misfolding of the proteins cell. The misfolding was ensue obstacle of apoptosis and to raise cell proliferation which to storm carcinogenesis. An up regulated of Hsp40 was role as co-chaperone.DOI: 10.14693/jdi.v16i2.91 
UJI VALIDITAS, REALIBILITAS HASIL PENGUKURAN BERAT BADAN MENGGUNAKAN RUMUS JUNG DENGAN TIMBANGAN ELEKTRONIK PADA PASIEN STROKE LANJUT USIA Jabar, Laode Abdul; Prayitno, Adi; Pancarini, Ety
Jurnal Kesehatan Kusuma Husada Vol. 9 No. 2, Juli 2018
Publisher : STIKes Kusuma Husada Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34035/jk.v9i2.277

Abstract

Pasien stroke akut seringkali tidak memiliki waktu dan sarana yang memadai untuk melakukan penimbangan berat badan pasien yang sebenarnya (aktual). Untuk pasien stroke tirah baring (bed rest), terpaksa harus di ukur menggunakan tempat tidur (hospital bed) khusus yang dilengkapi dengan timbangan berat badan elektronik. Akan tetapi, harganya sangat mahal dan tidak dimiliki oleh semua rumah sakit. Oleh karena itu, diperlukan instrumen yang bisa mengukur secara akurat dan tersedia secara luas di rumah sakit.Tujuan penelitian ini adalah untuk mengetahui validitas dan realibilitas hasil pengukuran berat badan menggunakan rumus Jung dengan timbangan elektronik pada pasien stroke lanjut usia. Jenis penelitian ini adalah observasional dengan desain cross sectional. Penelitian dilaksanakan pada bulan Mei 2017 sebanyak 94 pasien stroke rawat jalan di poliklinik Neuro RSUD dr. Moewardi Surakarta dilakukan pengukuran berat badan aktual menggunakan timbangan elektronik yang sebelumnya telah dikalibrasi ke terdekat 0,1 kg. Selanjutnya, dilakukan pengukuran lingkar lengan atas dan tinggi lutut yang kemudian dimasukkan kedalam rumus estimasi berat badan lansia Jung. Hasil penelitian ini adalah Rumus jung memiliki validititas yang baik terhadap timbangan elektronik pada pengkuruan berat badan pasien stroke lansia yang dapat dilihat pada nilai AUC sebesar 88,91% pada taraf signifikansi (a = 0,05) yaitu p-value = 0,000 < 0,05. Rumus jung terbukti realible/handal digunakan untuk pengkuruan berat badan pada pasien stroke lansia. Hal ini ditunjukkan dengan nilai AUC > 0,5 sebesar 12,084 (sig) p= 0,000 lebih kecil pada taraf signifikan a = 5%. Kesimpulan penelitian ini adalah Rumus Jung baik digunakan pada pengukuran berat badan pasien stroke. Acute stroke patients often do not have sufficient time and facilities for actual patient weight balancing (actual). For bed rest stroke patients, forced to be measured using a bed (hospital bed) special equipped with electronic weighing scales. However, the price is very expensive and is not owned by all hospitals. Therefore, an instrument that can measure accurately and widely available in the hospital is required. Objective of this study is to determine the validity and reliability of the results of weight measurements using Jung's formula with electronic scales in elderly stroke patients. The type of this study was observational with cross sectional design. This studi was conducted During May 2017 as many as 94 outpatient stroke patients in polyclinic Neuro RSUD dr. Moewardi Surakarta conducted actual weight measurement using electronic scales that had previously been calibrated to the nearest 0.1 kg. Furthermore, the measurement of the upper arm circumference and knee height is then incorporated into the formula of Jung's elderly weight estimate. Result this study the jung formula has good validity to the electronic scales in aging body weight of elderly stroke patients which can be seen in AUC value of 88,91% at significance level (a = 0,05) that is p-value = 0,000 <0,05. The proven realible / reliable junior formula is used for weight gain in elderly stroke patients. This is indicated by the value of AUC> 0.5 for 12.084 (sig) p = 0.000 smaller at significant level a = 5%. Conclusionthis study is Jung's formula is good for measuring the weight of stroke patients.
Geographical Satellite and Survey Data for Prediction of Dengue Cases in Sukoharjo, Indonesia Kusumawati, Dyah; Prayitno, Adi; Dharmawan, Ruben
Journal of Epidemiology and Public Health Vol 1, No 1 (2016)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Background: Dengue fever is a disease based on environment and still a health problem. Problems related to the dengue fever vector distribution factor in terms of the spread of vector space with the use of geographic data and survey data in order to predict the incidence of dengue in the region.Subjects and Methods: This study used analytic observational with cross sectional approach using modeling Geographical Information Systems (GIS). The sampling technique in this research is saturated sampling of secondary data Sukoharjo District Health Profile in 2011-2014, population data and data Geographic, then all the data were analyzed using multiple linear regression.Results: There is a positive relationship between the area per Km2 with the number of new cases of dengue fever, although the relationship was not statistically significant. (B = <0:01; CI -0.01 - 0:02; p = 0.310). There is a positive relationship between population density per soul / Km2dengan number of new cases of dengue fever, a significant relationship between population density with DHF cases. (B = <0:01; CI <0:01 to 0:01; p = 0.013). There is a negative relationship between topography per masl by the number of new cases of dengue fever, although the relationship was not statistically significant. (B = <0:01; CI -0.02 - 0:01; p = 0.335). There is a positive correlation between rainfall per mm / yr with the number of new cases of dengue fever, although the relationship was not statistically significant. (B = <0:01; CI <0:01 to 0:01; p = 0101). There is a positive relationship between river flow per ha by the number of new cases of dengue fever, although the relationship was not statistically significant. (B = 0:02; CI -0.01 - 0:03; p = 0318). There is a negative correlation between% Non Flick figure by the number of new cases of dengue fever, although the relationship was not statistically significant. (B = <0:01; CI -0.02 - 0:01; p = 0764).Conclusions: The increase in land area, population density, rainfall, river flow is predicted to affect the increase in dengue cases, whereas the increase ABJ predicted topography and affecting the decline of dengue cases in the district of Sukoharjo in 2011-2014.Keywords: geographical data and survey data, prediction of dengue casesCorrespondence: Dyah Kusumawati. Academy of Health Analyst 17  Agustus 1945, Semarang, Indonesia.  Email: dyahkusumawatiwinarno@yahoo.co.id. Mobile: 085876563978Journal of Epidemiology and Public Health (2016), 1(1): 11-17https://doi.org/10.26911/jepublichealth.2016.01.01.02                                                                                     
Implementation Analysis of Early Detection and Intervention Program for Growth and Development of Children Under Five at Tegal Health Centers Naharani, Adrestia Rifki; Joebagio, Hermanu; Prayitno, Adi
Indonesian Journal of Medicine Vol 1, No 3 (2016)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Background: The first five years of life is often considered as golden period,  critical period, or window opportunity. During this period there is maximal growth and development. Early Detection and Intervention Program for Growth and Development of Children Under Five (SDIDTK) is a program that aims to do early detection and intervention when there is an impairment in growth and development in children under five or pre-school children. Early detection allows early intervention. This study aimed to analyze the implementation of early detection and intervention program for growth and development of children under five.Subjects and Method: This was a qualitative study with case study approach, conducted in Tegal Health Centers, Tegal, Central Java. Nineteen key informants were selected purposely for this study, including health center midwife, midwife who were in charge of coordinating child and adolescents affair, head of the health center,  head of Children and Adolescents Division at District Health Office Tegal, and mothers who had children with growth disorder.Results: SDIDTK has been implemented since 2010. The communication aspect has not been implemented consistently. There was a lack in resources, including personnel, equipment, and  infrastructure. The attitude of the program implementer was not positive, because they considered that program was not effective. Guideline book and Standard Operating Procedure (SOP) were available. Growth examination activities were sufficient, but development examination activities were lacking. The reporting system was not sufficiently implemented.Conclusion: SDIDTK program was not been implemented optimally in Tegal. It is sugested to enhance the motivation of the program implementer and improve the equipment and they infrustructure for SDIDTK implementation.Keywords: implementation, SDIDTK program, growth, development, children under fiveCorrespondence: Adrestia Rifki Naharani. Masters Program in Public Health Sebelas Maret University, SurakartaIndonesian Journal of Medicine (2016), 1(3): 175-182https://doi.org/10.26911/theijmed.2016.01.03.05
Multilevel Analysis on the Factors Associated with Low Birth Weight in Temanggung, Central Java Khayati, Yulia Nur; Prayitno, Adi; Poncorini, Eti
Journal of Maternal and Child Health Vol 1, No 1 (2016)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Background: Infants with low birth weight or LBW is one of the risk factors for infant mortality. Complications LBW actually can be prevented and dealt with, but is constrained by access to health care, socio-economic circumstances, a referral system that has not gone well, delays in early detection and awareness of parents to seek medical help. By looking at the number of deaths caused by LBW remains high, and research on the causes of LBW widely used partial analysis, and have not been analyzed in stages, the researchers conducted this research with multilevel analysis.Subjects and Method: This was an observational studt with case control design. A total sample of 120 infants were selected by fixed disease sampling with a ratio of 1: 2 between cases and controls. Data were analyzed using multiple logistic regression.Results: There are three variables at the individual level were significantly associated with LBW and was statistically significant can among others, mother’s education (OR= 0.19; 95% CI= 0:07 to 0.53; p= 0.001), history of ANC t (OR= 7.76; 95% CI= 2.18 to 27.62; p= 0.002) and the nutritional mother status (OR= 5.61; 95% CI= 0.21 to 0.79; p= 0.008) and the variables that are not statistically significant is the mother’s age and family income, and there are  no contextual role within house with wellness facilities with LBW expressed by ICC < 0.001.Conclusion: The influence of maternal education, a history of the ANC examination and nutritional status of mothers with LBW and there is no contextual role of distance between home and health care facilities with LBW. This study suggests to health professional to improve the coverage of the ANC.Keywords: multilevel analysis, factor low birth weightCorrespondence: Yulia Nur Khayati. School of Health and sciences, STIKes Ngudi Waluyo, Ungaran, Indonesia. Email: yulia.farras@gmail.com.Journal of Maternal and Child Health (2016), 1(1): 7-12https://doi.org/10.26911/thejmch.2016.01.01.02
Association betweenMaternal Age at Pregnancy, Socioeconomic Status, Physical Environment, Prenatal, Perinatal, Postnatal History, and the Risk of Mental Retardation Nurochim, Erna; Indarto, Dono; Prayitno, Adi
Journal of Maternal and Child Health Vol 1, No 2 (2016)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Background: Mental retardation (MR) is a serious public health problem for an country. The prevalence of mental retardation in Indonesia was estimated at 1-3% of the population. About 0.1% of whichneed treatment and guidance the whole life. This study aimed to determine the association between maternal age at pregnancy, socioeconomic status, physical environment, prenatal, perinatal, postnatal history, and mental retardation.Subject and methods: This was an analytic observational study with case control design. This study was conducted in Pare, Kediri, East Java, Indonesia,from October to December 2016. A total 105 study subjects, consisting of 35 MR cases and 70 controls were selected for this study by fixed disease sampling. The independent variables were maternal age at pregnancy, socioeconomic status, physical environment, prenatal, perinatal, and postnatal history. The dependent variable was mental retardation. The data were collected by questionnaire and analyzed by logistic regression model.Results: Maternal age ≥35 years at pregnancy was associated with an increased risk of MR, and it was statistically significant The result showed that mother’s age while pregnant (OR=10.18; 95%CI=2.77 to 37.39; p<0.001). High socioeconomic status (OR=0.23;95%CI=0.07 to 0.81; p=0.022), good physical environment (OR=0.13; 95%CI=0.04 to 0.45; p=<0.001), good prenatal history (OR=0.24;95%CI=0.07 to 0.82; p=0.022), good perinatal history (OR=0.45;95%CI=0.15 to 1.40; p=0.168), good postnatal (OR=0.43;95%CI=0.14 to 1.35; p=0.148), were associated with a decreased risk of MR. Nagelkerke R2=53.8% for this model.Conclusion: Maternal age ≥35 years at pregnancy increases the risk of MR. High socioeconomic status, good physical environment, good prenatal, perinatal, and postnatal history, decrease the risk of MR.Keywords: mental retardation, maternal age at pregnancy, socioeconomic status, environment, pregnancy history.Correspondence: Erna Nurochim. Masters Program in Public Health, Sebelas Maret University, Surakarta.Journal of Maternal and Child Health (2016), 1(2): 119-130https://doi.org/10.26911/thejmch.2016.01.02.07
To reduction the primary dysmenorrhea on adolescent girl stressed with mung bean extract drinks and stretching Septiani, Baiq Dewi Sukma; Prayitno, Adi; Sugiarto, Sugiarto
International Journal of Public Health Science (IJPHS) Vol 8, No 1: March, 2019
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.11591/ijphs.v8i1.14852

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Primary dysmenorrhea is pain during menstruation period due to high levels of prostaglandins, especially prostaglandin F2α in epithelial cells. MungBean Extract Drinks contain several nutrients including vitamin C, calcium, carbohydrates and flavonoids as anti-depressants and anti-inflammatory agent. Stretching is a relaxation technique that can helps relieve menstrual pain in the abdominal part by increasing the production of endorphins which function as neurotransmitters. To determine the effect of Mung Bean ExtractDrinks and Stretching on primary dysmenorrhea (primary menstrual pain) in adolescent girls stressed. This type of open-label non-randomized controlled trial used 87female adolescents who experienced primary menstrual pain with moderate and severe stress levels from two Islamic boarding schools in Mataram City, West Nusa Tenggara, Indonesia, which were divided into 3 groups, one group as the control and two groups as the experiment. The experiment of 29people with pre and post-test. Mung bean extract drinks were given 250ml/day, otherwise stretching was given 1x/day for 7days before menstruation. Control and experimental group was observed for 1 month. Measurement of menstrual pain scale using the Numeric Rating Scale (NRS) assessment sheet. Data analysis uses T-test. The mean primary menstrual pain in adolescents for MungBean Extract Drinks with moderate stress levels was 1.31±1.32 and the difference before and after mung bean extract drinks experience is significant at p=0.004 while at severe stress levels was 1.69±1.54 and the difference between before and after mung bean extract drinks experience is significant at p=0.001. The mean primary menstrual pain in adolescents for stretching experience with moderate stress levels was 2.50±1.93 and the difference before and after stretching experience is significant at p=0.008 while the severe stress level was 2.05±1.80 and the difference before and after stretching experience is significant at p≤0.001. The multivariate test with linear regression revealed that mung bean extract drinks contributed y= (-)138X1+4.897(p=0.001) and related stretching contributed y= (-)2.517X1+6.276(p=0.001). MungBean ExtractDrinks and Stretching will affect to the reduction of primary dysmenorrhea in adolescent girls both at moderate and severe stress levels