Bogi Pratomo
Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, University of Brawijaya/Dr. Saiful Anwar General Hospital, Malang

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Journal : Jurnal Penyakit Dalam Indonesia

Pengaruh Kurkumin Terhadap Kadar NF-κB dan Derajat Fibrosis Hati pada Tikus Fibrosis Hati Supriono, Supriono; Pratomo, Bogi; Praja, Dedy Indra
Jurnal Penyakit Dalam Indonesia Vol 5, No 4 (2018)
Publisher : Internal Medicine Department, Faculty of Medicine Universitas Indonesia-RSCM

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Abstract

Pendahuluan. Inflamasi kronik merupakan mekanisme utama penyebab fibrosis hati. NF-κB  berfungsi mengatur inflamasi, penyembuhan luka, serta kematian sel. Kurkumin berperan sebagai antiinflamasi, antifibrotik, dan induksi apoptosis, salah satunya melalui hambatan terhadap NF-κB. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian kurkumin terhadap perubahan kadar NF-κB dan korelasi antara lama pemberian kurkumin terhadap kadar NF-κB dan derajat fibrosis hati.Metode. Penelitian ini merupakan penelitian eksperimental in vivo menggunakan 32 ekor tikus wistar yang dibagi dalam 8 kelompok perlakuan, tiap kelompok terdiri dari 4 ekor tikus. Perlakuan terhadap tikus berupa induksi CCl4 dan pemberian kurkumin. Pengukuran kadar NF-κB dengan metode ELISA. Derajat fibrosis hati menggunakan Metavir scoring system. Analisis data menggunakan uji korelasi, one-way Anova, unpair T-test, Mann-Whitney, Chi-square, Kruskal-Wallis dan analisis jalur model struktural dengan nilai p signifikan yaitu p<0,05. Hasil. Terdapat perbedaan yang signifikan kadar NF-κB dan derajat fibrosis hati antara kelompok kontrol positif dengan kelompok kontrol negatif. Terdapat pengaruh yang signifikan pemberian kurkumin dan lama pemberian kurkumin terhadap perubahan derajat fibrosis hati dan kadar NF-κB jaringan hati. Terdapat korelasi negatif antara lama pemberian kurkumin dengan perubahan kadar NF-κB dan korelasi positif antara perubahan kadar NF-κB dengan derajat fibrosis hati.Simpulan. Pemberian kurkumin dapat menurunkan kadar NF-κB dan derajat fibrosis hati. Lama pemberian kurkumin berkorelasi dengan penurunan kadar NF-κB dan penurunan kadar NF-κB berkorelasi dengan penurunan derajat fibrosis hati. Kata Kunci: Derajat fibrosis hati, Kurkumin, NF-κB, Tikus model fibrosis hati The Effects of Curcumin on NF-κB Level and Degree of Liver Fibrosis in Rat Liver Fibrosis Introduction. Chronic inflammation is the main mechanism responsible for liver fibrosis. NF-κB regulates inflammation, wound healing, and cell death. Curcumin acts as an antiinflammatory, antifibrotic and induced apoptosis, one of them through resistance to NF-κB. This study aimed to determine the effect of curcumin on NF-κB levels and the correlation between the duration of curcumin on NF-κB and degree of liver fibrosis. Methods. This study was an experimental in rats using a completely randomized design. The treatment of rats was induced with CCl4 and given curcumin. Measurement of NF-κB levels by ELISA method. Degree of liver fibrosis using metavir scoring system. The data were analyzed using correlation test, one-way anova, unpair T-test, mann-whitney, chi-square, kruskal-wallis, structural model with significant p value p < 0,05. Results. There were significant differences in NF-κB level and degree of liver fibrosis between the positive control group and the negative control group. There was a significant effect of curcumin administration on the level of NF-κB and degree of liver fibrosis. There was a negative correlation between duration of curcumin and NF-κB level and positive correlationbetween NF-κB levels and degree of liver fibrosis. Conclusions. Curcumin can decrease NF-κB levels and decrease the degree of liver fibrosis. The duration of the curcumin administration correlated with decreased levels of NF-κB and decreased NF-κB levels correlated with decreased degree of liver fibrosis in rat liver fibrosis. 
Analisis Faktor Risiko Gastroesofageal Refluks di RSUD Saiful Anwar Malang Tarigan, Ricky; Pratomo, Bogi
Jurnal Penyakit Dalam Indonesia Vol 6, No 2 (2019)
Publisher : Internal Medicine Department, Faculty of Medicine Universitas Indonesia-RSCM

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Abstract

Pendahuluan. Gastroesophageal reflux disease (GERD) adalah suatu keadaan patologis sebagai akibat refluks kandungan lambung ke dalam esofagus dengan berbagai gejala yang timbul akibat keterlibatan esofagus, laring, dan saluran nafas. Prevalensi GERD meningkat akhir-akhir ini. Di Indonesia, ditemukan kasus esofagitis sebanyak 22,8%. Analisis faktor risiko terjadinya GERD sangatlah penting diketahui di dalam mengurangi prevalensi GERD. Tujuan dari penelitian ini yaitu untuk mengetahui faktor risiko yang paling berpengaruh pada pasien GERD sehingga diharapkan menjadi acuan referensi untuk mengurangi prevalensi GERD di tahun berikutnya.Metode. Penelitian ini merupakan penelitian cross-sectional. Data yang diambil berdasarkan rekam medis pasien yang menjalani pemeriksaan endoskopi di Divisi Gastroenterologi Hepatologi di Rumah Sakit Dr. Saiful Anwar Malang selama tahun 2016. Diagnosis didapatkan dari anamnesis, pemeriksaan fisik, dan kriteria diagnostik GERD dari endoskopi menurut kriteria Los Angeles.Hasil. Didapatkan 57 pasien dengan wanita 20 orang (32,5%) dan laki-laki 37 orang (67,5%). Pasien berusia >40 tahun ada sebanyak 36 pasien (63,16%). Didapatkan sebanyak 28 pasien (49,12%) mengonsumsi jamu, 31 pasien (54,38%) merokok, dan 18 pasien (31,48%) mengonsumsi alkohol. Hasil analisis faktor risiko GERD menunjukkan bahwa faktor yang berpengaruh terhadap kejadian GERD pada yaitu faktor konsumsi jamu (p=0,007 dengan nilai OR=4,586 (interval kepercayaan [IK] 95%: 1,386-15,177)) dan alkohol (p=0,027 dengan nilai OR=4,846 (IK 95%: 1,024-22,929)).Simpulan. Faktor yang berpengaruh terhadap kejadian GERD pada penelitian ini yaitu faktor konsumsi jamu dan alkohol.Kata Kunci: Endoskopi, Gastroesofageal refluks, GERD, Kriteria Los Angeles  Gastroesophageal Reflux Risk Factor Analysis at Saiful Anwar Hospital in MalangIntroduction. Gastroesophageal reflux disease (GERD) is a pathological condition as a result of reflux of stomach contents into the esophagus with various symptoms that arise due to the involvement of the esophagus, larynx, and airways. The prevalence of GERD has increased lately. In Indonesia, 22.8% cases of esophagitis were reported. Analysis of the risk factors for GERD is very important in reducing the prevalence of GERD. This study aimed to identify the most influential risk factors for GERD patients so that it could become a reference to reduce the prevalence of GERD in the following year.Methods. This study was a cross-sectional study where data was taken based on data from medical records of patients undergoing the endoscopic procedure in the division of Hepatology Gastroenterology at Saiful Anwar Hospital Malang during 2016. The diagnosis was obtained from history, physical examination, and GERD diagnostic criteria from endoscopy according to the Los Angeles criteria.Results. Among 57 patients, there were 20 female (32.5%) and 37 male (67.5%), 63.16% of them are >40 years old. This study found that 28 patients (49.12%) consumed herbs, 31 patients (54.38%) found smoking, and those who consumed alcohol were 18 patients (31.48%). The analysis showed that factors related to the incidence of GERD were herbal consumption (p=0.007; OR 4.586 (95% CI: 95%: 1.386-15.177)) and alcohol consumption (p 0.027; OR 4.846 (95% CI: 1.024-22.929)). Conclusion. Consumption of herbal and alcohol appear to be risk factors of the incidence of GERD in this study.