Ratih Hardika Pratama
Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta

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Taraxacum officinale Leaves Ethanolic Extract as Immunostimulatory Agent For Reducing Side Effect of Doxorubicin in Sprague Dawley Rats Kasianningsih, Sri; Rivanti, Erlina; Pratama, Ratih Hardika; Pratama, Nanda Resa; Ikawati, Muthi; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Doxorubicin as chemotherapeutic agent causes immunosuppresive. The aim  for this study to determine the effect of ethanolic extract of Taraxacum oficinale (ETO) in immunity system of Sprague Dawley  rat that induced  by doxorubicin to observe the profile of immunity cells. Sprague Dawley rats were divided into five groups each groups contain five rats : control doxorubicin group, doxorubicin dose 4,67 mg/kgBW+ ETO dose 1000 mg/kgBW, doxorubicin dose 4,67 mg/kgBW+ ETO dose 500 mg/kgBW, control extract group, and without treatment. Then the number of leukocytes, lymphocytes and neutrophils were analyzed  by hematology analyzer, whereas CD8+ T lymphocytes by flowcytometry. Results showed groups of  doxorubicin combined with ETO dose 1000 mg/kgBW and 500 mg/kgBW increased the number of leukocytes, lymphocytes, neutrophils,  cytotoxic CD8 + T cells  T cells compared  to control doxorubicin group. These data presents that etanolic extract of Jombang leaves has  immunostimulatory activity and potential as co-chemotherapy agents. Molecullar mechanism underlaying it’s immune activity need to be explored in detail.
Ekstrak Etanolik Daun Awar-Awar (Ficus septica Burm F.) secara Sinergis Meningkatkan Efektivitas Doxorubicin terhadap Sel Kanker Payudara T47D PRATAMA, RATIH HARDIKA; IKHTIARSYAH, YURISTA GILANG; ANINDYAJATI, ANINDYAJATI; FITRIASARI, ADTYA; IKAWATI, MUTHI; MEIYANTO, EDY
JURNAL ILMU KEFARMASIAN INDONESIA Vol 9 No 1 (2011): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

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Abstract

Daun awar-awar (Ficus septica Burm E), yang belum dimanfaatkan secara optimal dalam pengobatan kanker, memiliki potensi untuk digunakan sebagai agen kombinasi dengan doxorubicin. Penelitian ini bertujuan untuk mengetahui efek sitotoksik ekstrak etanolik daun awar-awar (EDA) dan kombinasinya dengan agen kemoterapi doxorubicin (DOX) pada sel kanker payudara T47D. EDA diperoleh dari maserasi serbuk kering daun awar-awar dengan etanol 70%. Pengujian aktivitas sitotoksik ekstrak dilakukan dengan MT T assay, baik perlakuan tunggal maupun kombinasinya dengan DOX. Sifat sitotoksik ditentukan dengan nilai IC50, sementara efektivitas kombinasi dihitung dengan nilai indeks kombnasi (CI) untuk menetapkan apakah efeknya sinergis, aditif, atau antagonis. Uji sitotoksik perlakuan EDA tunggal selama 24 jam menunjukkan efek sitotoksik yang potensial dengan IC50 sebesar 13 µg/mL. Kombinasi EDA-DOX menunjukkan efek sinergis (CI < 1) pada konsentrasi EDA 4,88 µg/mL dan DOX 3.75 nM. Hasil ini menunjukkan bahwa EDA memiliki potensi yang menjanjikan untuk diaplikasikan sebagai agen ko-kemoterapi DOX pada terapi kanker payudara.
Taraxacum officinale Leaves Ethanolic Extract as Immunostimulatory Agent For Reducing Side Effect of Doxorubicin in Sprague Dawley Rats Kasianningsih, Sri; Rivanti, Erlina; Pratama, Ratih Hardika; Pratama, Nanda Resa; Ikawati, Muthi'; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (655.334 KB) | DOI: 10.14499/indonesianjcanchemoprev2iss1pp135-140

Abstract

Doxorubicin as chemotherapeutic agent causes immunosuppresive. The aim for this study to determine the effect of ethanolic extract of Taraxacum oficinale (ETO) in immunity system of Sprague Dawley rat that induced by doxorubicin to observe the profile of immunity cells. Sprague Dawley rats were divided into five groups each groups contain five rats: control doxorubicin group, doxorubicin dose 4,67 mg/kgBW+ ETO dose 1000 mg/kgBW, doxorubicin dose 4,67 mg/kgBW+ ETO dose 500 mg/kgBW, control extract group, and without treatment. Then the number of leukocytes, lymphocytes and neutrophils were analyzed by hematology analyzer, whereas CD8+ T lymphocytes by flowcytometry. Results showed groups of doxorubicin combined with ETO dose 1000 mg/kgBW and 500 mg/kgBW increased the number of leukocytes, lymphocytes, neutrophils,  cytotoxic CD8 + T cells T cells compared to control doxorubicin group. These data presents that etanolic extract of Jombang leaves has immunostimulatory activity and potential as co-chemotherapy agents. Molecullar mechanism underlaying it’s immune activity need to be explored in detail.Keywords: co-chemotheraphy, doxorubicin, immunostimulatory, in vivo Taraxacum officinale