Diding H. Prasetyo
Divisi Imunologi-Laboratorium Biokimia Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Ekstrak Etanol Propolis Menurunkan Kadar IL-17 Serum pada Mencit Balb/C Model Asma Kronik

Majalah Kedokteran Bandung Vol 45, No 4 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Interleukin-17 (IL-17) menginduksi perekrutan neutrofil ke dalam saluran napas dan berperan dalam patogenesis asma serta penentuan keparahan penyakit. Tujuan penelitian ini adalah menganalisis efek ekstrak etanol propolis (EEP) isolat Gunung Lawu terhadap kadar IL-17 serum pada mencit Balb/C model asma kronik. Penelitian ini merupakan penelitian eksperimental laboratoris dengan sampel 28 ekor mencit Balb/C jantan yang dibagi menjadi empat kelompok: kontrol, asma, asma+EEP, dan asma+antihistamin. Penelitian dilakukan di Laboratorium Histologi dan Biomedik, Fakultas Kedokteran, Universitas Sebelas Maret, Surakarta, selama periode April-September 2012. Model asma kronik dibuat dengan menginduksi mencit Balb/C jantan menggunakan injeksi intraperitoneal (i.p.) ovalbumin (OVA, 100 μg/mencit) dan aluminium hidroksida (10%, 50 mg/mencit) dalam phosphate-buffered saline (PBS). Hewan coba disensitisasi selama 6 minggu dengan inhalasi 2% (b/v) OVA aerosol dalam NaCl 0,9% selama 30 menit/hari setiap 3 hari/minggu, untuk kelompok asma, asma+EEP, dan asma+antihistamin. Mencit kontrol tidak diinokulasi selama penelitian. Deteksi kadar IL-17 serum menggunakan enzyme-linked immunosorbent assay (ELISA). Uji kadar IL-17 serum menggunakan analysis of variance (ANOVA) dan untuk menentukan perbedaan kemaknaan digunakan p<0,05. Kadar IL-17 serum kelompok kontrol menunjukkan 17,15±8,25 pg/mL dan antihistamin 38,98±7,57 pg/mL. Ekstrak etanol propolis secara bermakna menurunkan kadar IL-17 serum (75,62±17,64 pg/mL vs 45,59±10,69 pg/mL, p<0,001) dibandingkan dengan kelompok asma. Simpulan, EEP isolat Gunung Lawu menurunkan kadar IL-17 pada hewan coba model asma kronik. [MKB. 2013;45(4):213–7]Kata kunci: Asma, interleukin-17 (IL-17), propolisEthanolic Extract of Propolis Decreases Serum IL-17 Levels in Balb/C Model of Chronic AsthmaInterleukin-17 (IL-17) induces the neutrophil recruitment into the respiratory tract while playing a role in the pathogenesis of asthma and determining the severity of disease. The aim of this study was to analyze the effect of ethanol extract of propolis (EEP) Lawu mountain isolates on IL-17 serum levels in Balb/C mice model with chronic asthma. This study was an experimental research laboratory with 28 male Balb/C mice divided into control, asthma, asthma+EEP and asthma+antihistamine groups. The study was conducted at the Histology and Biomedical Laboratory, Faculty of Medicine, Sebelas Maret University, Surakarta, during April to September 2012. A model of chronic asthma was induced in male Balb/C mice using an intraperitoneally (i.p.) injection ovalbumin (OVA, 100 μg/mice) and aluminum hydroxide (10%, 50 mg/mice) in phosphate-buffered saline (PBS). Experimental animals were sensitized for 6 weeks with inhaled 2% (w/v) OVA aerosol in 0.9% NaCl for 30 minutes/day, 3 days in a week, for asthma, asthma with EEP, and asthma with antihistamine mice model groups. Control mice were not inoculated during the study. Detection of IL-17 serum level was conducted by using enzyme-linked immunosorbent assay (ELISA). One way analysis of variance (ANOVA) for IL-17 serum level and p<0.05 were used to determine significant differences. Serum levels of IL-17 showed 17.15±8.25 pg/mL in control group and 38.98±7.57 pg/mL in antihistamine, respectively. Ethanol extract of propolis showed significant decreased in IL-17 levels (75.62±17.64 pg/mL vs 45.59±10.69 pg/mL, p<0.001) compared to asthma group. In conclusion, EEP Lawu mountain isolates can decrease IL-17 level in chronic asthma animal models. [MKB. 2013;45(4):213–7]Key words: Asthma, interleukin-17 (IL-17), propolis DOI: http://dx.doi.org/10.15395/mkb.v45n4.167

Molecular Therapeutic Potency of Metformin by Targeting p53-Related Molecules in Mutant p53 Colon Cancer Cell Line

Indonesian Journal of Cancer Chemoprevention Vol 7, No 1 (2016)
Publisher : Indonesian Society for Cancer Chemoprevention

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Abstract

Colon cancer is a malignancy in gastrointestinal tract. It causes high mortality rate in global cancer population. However, chemotherapy as its first option therapy is still controversial due to its effectiveness and its adverse effects. Finding supportive and alternative drugs to cure cancer is one of focus in cancer research. A drug which also has anticancer effects is metformin. Metformin is a biguanide antidiabetic which show its potential anticancer benefit in metabolic-related cancers including colon cancer. To investigate anticancer potency of metformin in targeting p53-related molecules. Metformin treatment were divided into 4 groups by 0, 5, 10 and 20 mM concentrations and incubated in 37o C and 5 % CO2 condition for 48 hours. Immunohistochemistry were conducted to asses level of expression of Bax, p21, cyclin D1 and E2F1, respectively. Level of expression were measured by H-SCORE using percentage and intensity calculation. Comparisons of H-SCORE between groups were performed by ANOVA for parametrical data and Kruskal-Wallis for non-parametrical data. Growth inhibition were observed after metformin treatment. Metformin increases Bax expression significantly at all concentrations. p21 expression was also increased after metformin treatment but is not statistically significant. Subsequently, metformin decreases cyclin D1 expression at 10 and 20 mM concentration thus decreased E2F1 expression at 5 and 10 mM concentration. These data suggest that metformin may have potential therapeutic effects in mutant p53 colon cancer cell line by targeting p53-related molecules.Keywords: Colon cancer; p53; Biguanide; Metformin; p53-mutant cell line

Ekstrak Etanol Propolis Menurunkan Kadar IL-17 Serum pada Mencit Balb/C Model Asma Kronik

Majalah Kedokteran Bandung Vol 45, No 4 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Original Source | Check in Google Scholar

Abstract

Interleukin-17 (IL-17) menginduksi perekrutan neutrofil ke dalam saluran napas dan berperan dalam patogenesis asma serta penentuan keparahan penyakit. Tujuan penelitian ini adalah menganalisis efek ekstrak etanol propolis (EEP) isolat Gunung Lawu terhadap kadar IL-17 serum pada mencit Balb/C model asma kronik. Penelitian ini merupakan penelitian eksperimental laboratoris dengan sampel 28 ekor mencit Balb/C jantan yang dibagi menjadi empat kelompok: kontrol, asma, asma+EEP, dan asma+antihistamin. Penelitian dilakukan di Laboratorium Histologi dan Biomedik, Fakultas Kedokteran, Universitas Sebelas Maret, Surakarta, selama periode April-September 2012. Model asma kronik dibuat dengan menginduksi mencit Balb/C jantan menggunakan injeksi intraperitoneal (i.p.) ovalbumin (OVA, 100 μg/mencit) dan aluminium hidroksida (10%, 50 mg/mencit) dalam phosphate-buffered saline (PBS). Hewan coba disensitisasi selama 6 minggu dengan inhalasi 2% (b/v) OVA aerosol dalam NaCl 0,9% selama 30 menit/hari setiap 3 hari/minggu, untuk kelompok asma, asma+EEP, dan asma+antihistamin. Mencit kontrol tidak diinokulasi selama penelitian. Deteksi kadar IL-17 serum menggunakan enzyme-linked immunosorbent assay (ELISA). Uji kadar IL-17 serum menggunakan analysis of variance (ANOVA) dan untuk menentukan perbedaan kemaknaan digunakan p<0,05. Kadar IL-17 serum kelompok kontrol menunjukkan 17,15±8,25 pg/mL dan antihistamin 38,98±7,57 pg/mL. Ekstrak etanol propolis secara bermakna menurunkan kadar IL-17 serum (75,62±17,64 pg/mL vs 45,59±10,69 pg/mL, p<0,001) dibandingkan dengan kelompok asma. Simpulan, EEP isolat Gunung Lawu menurunkan kadar IL-17 pada hewan coba model asma kronik. [MKB. 2013;45(4):213–7]Kata kunci: Asma, interleukin-17 (IL-17), propolisEthanolic Extract of Propolis Decreases Serum IL-17 Levels in Balb/C Model of Chronic AsthmaInterleukin-17 (IL-17) induces the neutrophil recruitment into the respiratory tract while playing a role in the pathogenesis of asthma and determining the severity of disease. The aim of this study was to analyze the effect of ethanol extract of propolis (EEP) Lawu mountain isolates on IL-17 serum levels in Balb/C mice model with chronic asthma. This study was an experimental research laboratory with 28 male Balb/C mice divided into control, asthma, asthma+EEP and asthma+antihistamine groups. The study was conducted at the Histology and Biomedical Laboratory, Faculty of Medicine, Sebelas Maret University, Surakarta, during April to September 2012. A model of chronic asthma was induced in male Balb/C mice using an intraperitoneally (i.p.) injection ovalbumin (OVA, 100 μg/mice) and aluminum hydroxide (10%, 50 mg/mice) in phosphate-buffered saline (PBS). Experimental animals were sensitized for 6 weeks with inhaled 2% (w/v) OVA aerosol in 0.9% NaCl for 30 minutes/day, 3 days in a week, for asthma, asthma with EEP, and asthma with antihistamine mice model groups. Control mice were not inoculated during the study. Detection of IL-17 serum level was conducted by using enzyme-linked immunosorbent assay (ELISA). One way analysis of variance (ANOVA) for IL-17 serum level and p<0.05 were used to determine significant differences. Serum levels of IL-17 showed 17.15±8.25 pg/mL in control group and 38.98±7.57 pg/mL in antihistamine, respectively. Ethanol extract of propolis showed significant decreased in IL-17 levels (75.62±17.64 pg/mL vs 45.59±10.69 pg/mL, p<0.001) compared to asthma group. In conclusion, EEP Lawu mountain isolates can decrease IL-17 level in chronic asthma animal models. [MKB. 2013;45(4):213–7]Key words: Asthma, interleukin-17 (IL-17), propolis DOI: http://dx.doi.org/10.15395/mkb.v45n4.167