Articles

Estimasi Produk Degradasi Ekstrak Kasar Pigmen Alga Merah Kappaphycus alvarezii (Doty) Doty Varian Merah, Coklat, dan Hijau: Telaah Perbedaan Spektrum Serapan de Fretes, Helly; Susanto, A.B.; Prasetyo, Budi; Heriyanto, Heriyanto; Brotosudarmo, Tatas H.P.; Limantara, Leenawaty
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 17, No 1 (2012): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Penelitian ini bertujuan untuk mengestimasi produk degradasi pigmen ekstrak kasar alga merah Kappaphycus alvarezii (Doty) Doty varian merah, coklat dan hijau yang terbentuk selama perlakuan iradiasi dan pemanasan, melalui perbedaan spektrum serapannya. Ketiga varian diekstraksi menggunakan 100% metanol. Uji fotostabilitas ekstrak pigmen dilakukan dengan iradiasi menggunakan lampu Volpi intralux 4100 pada intensitas cahaya 39300 lux, 56700 lux dan 76400 lux.  Lama waktu penyinaran 0, 5, 10, 15, dan 20 menit. Sedangkan uji termostabilitas  dilakukan pada suhu 25 dan 90 ° C selama 0, 3, 6 24, dan 48 jam. Pola spektra diukur pada panjang gelombang 300-800 nm sebelum dan sesudah perlakuan dengan spektrofotometer UV-Tampak Shimadzu 1700. Data dianalisis dengan SPINA Versi 3, untuk memperoleh intensitas maksimum dan perbedaan spektra serapan. Hasil penelitian menunjukkan bahwa produk degradasi pigmen ekstrak kasar alga merah K. alvarezii dapat diidentifikasi melalui spektrum serapan dan hasil perbedaan spektra serapan. Isomer cis karotenoid, karotenoid tidak berwarna, dan feofitin a diestimasi sebagai produk degradasi yang terbentuk selama perlakuan iradiasi dengan intensitas cahaya 76400 lux dan perlakuan pemanasan pada suhu 90°C selama 48 jam. Penurunan intensitas warna larutan pigmen juga mengindikasikan terbentuknya produk degradasi selama perlakuan. Kata kunci: Kappaphycus alvarezii, perbedaan spektrum serapan, produk degradasi The aims of this study were to estimate the degradation products of crude pigment extracts from red, brown, and green varieties of red alga Kappaphycus alverezii (Doty) Doty that formed during irradiation and heating treatment from their difference absorption spectra. All three variants were extracted using 100% methanol. The photostability assay of crude pigment extracts was conducted by irradiating the crude pigment extracts with a day light lamp (Volpi, Intralux 4100) at 39300 lux, 56700 lux and 76400 lux light intensity during 0, 5, 10, 15, and 20 minutes treatment, whereas the thermostability assay was performed at 25 and 90 °C for 0, 3, 6 24, and 48 hours. The absorption spectra of the crude pigment extract before and after each treatment were monitored successively at 300-800 nm using MultiSpec 1501 UV-VIS spectrophotometer. Data were analyzed with spina Version 3, to obtain maximum intensity and the difference absorption spectra. The results showed that the  degradation products of crude pigment extracts from red alga K. alvarezii could be identified by absorption spectra and difference absorption spectra. Cis isomers carotenoids, colorless carotenoids, and feofitin-a were estimated as degradation product formed during the irradiation treatment with 76400 lux of light intensity and heating treatment at a temperature of 90 ° C for 48 hours. Decrease in the intensity of the color of pigment solution also indicates the formation of degradation products during treatment.Key words: Kappaphycus alvarezii, difference absorption spectra, degradation products
PENGEMBANGAN MODEL ADAPTASI ROY PADA KONSEP DIRI PASIEN DENGAN PEMASANGAN FIKSASI EKSTERNAL DI RSO Prof. Dr. R. SOEHARSO SURAKARTA Prasetyo, Budi
HOSPITAL MAJAPAHIT (JURNAL ILMIAH KESEHATAN POLITEKNIK KESEHATAN MAJAPAHIT) Vol 6, No 2 (2014): HOSPITAL MAJAPAHIT VOL 6 NO 2
Publisher : HOSPITAL MAJAPAHIT (JURNAL ILMIAH KESEHATAN POLITEKNIK KESEHATAN MAJAPAHIT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.10475/hm.v6i2.473

Abstract

Installation of external fixation is the method of reduction and immobilization, for the handling of fractures. One of the impact of external fixation mounting is the change in self?concept. Self-concept according to Stuart (2005) consists of 5 components. Roy in his theory emphasizes the concept of adaptation in overcoming stressor. The study was conducted to explore the self-concept of the patient with the installation of an external fixation using Roy's adaptation model development. Research using phenomenological qualitative research design. The population was patients who received external fixation installation management (including Ilizarov) undergoing inpatient and outpatient care (control) in the RSO Prof. Dr. R. Soeharso Surakarta. Total sampling technique was used and the participants get 7 people. The experiment was conducted 4 weeks. Instruments using interviews and fields notes that have been tested for validity and reliability. Processing and data analysis using methods of data interpretation (Speziale & Carpenter, 2003) and software NVivo 9.0.204.0. Research carried out by applying the principles of research ethics. Generated 14 themes, which is an indication of mounting external fixation, development theory, significant other theory, self?perception theory, additional traits, coping mechanisms, body image, ideal self, self-esteem, role performance, personal identity, nursing care services, expectations of participants, and the difficulties experienced by participants. Concluded a negative self-concept changes (adaptation ineffective) on all participants. Roy adaptation model development in the assessment of changes in self-concept in the installation of external fixation in the hospital, very appropriately to used.Keywords: Roy adaptation model, self-concept, external fixation
RANCANG BANGUN PERPUSTAKAAN DIGITAL POLITEKNIK NEGERI SEMARANG BERBASIS WEB DENGAN FLEX FRAMEWORK Handoko, Slamet; Sulistiyo, Wahyu; Prasetyo, Budi; Annanuur, Fella
Jurnal Informatika Vol 5, No 2: Juli 2011
Publisher : Universitas Ahmad Dahlan

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Teknologi informasi dan komunikasi atau “ICT” (Information and Communication) telah menjadi bagian yang tak terpisahkan dari kehidupan global manusia, baik secara personal maupun organisasi atau institusi. Perpustakaan sebagai salah satu tempat sumber ilmu perlu ditata dan disajikan agar dapat diakses kapan dan dimanapun tanpa harus datang secara langsung ke perpustakaan dengan melibatkan teknologi ICT dalam bentuk suatu sistem yang terintegrasi. Pengembangan perpustakaan Politeknik Negeri Semarang (Polines) saat ini baru sampai pada tahap scanning/digitalisasi dokumen,  sehingga perlu ditata kembali dari sumber data yang ada untuk diolah lebih lanjut dalam bentuk perpustakaan digital yang dapat diakses secara global. Tujuan dari dibuatnya sistem perpustakaan digital Polines  ini untuk membantu penelusuran buku umum dan buku Tugas Akhir bagi mahasiswa Polines. Sistem ini dibangun dengan menggunakan aplikasi dari Adobe Flex Builder 4 dan PHP sebagai bahasa pemrograman,  dan database MySQL. Sistem ini diuji dengan melakukan pengujian sistem website untuk memastikan semua fungsi berjalan dengan baik. Disamping itu, diuji juga dengan menggunakan uji kepuasan pengguna terhadap sistem perpustakaan digital Polines ini. Dengan sistem informasi ini, pengguna semakin mudah untuk mencari referensi buku umum dan buku Tugas Akhir pada perpustakaan digital Polines.
DETEKSI GEN tst ISOLAT Staphylococcus aureus MELALUI AMPLIFIKASI 23S rRNA ASAL SUSU KAMBING DAN SAPI PERAH Prasetyo, Budi; Kusumaningrum, Elizabeth Novi
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (213.383 KB) | DOI: 10.21157/j.ked.hewan.v8i1.1266

Abstract

Penelitian ini bertujuan mendeteksi gen tst isolat Staphylococcus aureus (S. aureus) susu sapi perah dan susu kambing melalui amplifikasi gen 23S rRNA sebagai langkah awal pencegahan beberapa kasus keracunan susu. Metode penelitian meliputi reidentifikasi bakteri, preparasi deoxyribonucleic acid (DNA), amplifikasi gen 23S rRNA, amplifikasi gen tst, dan pengurutan DNA. Hasil reidentifikasi bakteri melalui pewarnaan Gram, uji katalase, uji koagulase, uji fermentasi MSA, uji VJA, dan uji VP adalah positif bakteri S. aureus. Hasil amplifikasi gen tst terhadap 3 isolat S. aureus menunjukkan hanya 2 isolat memberikan hasil positif. Hasil positif tersebut ditandai dengan munculnya fragmen DNA yang memiliki panjang spesifik (350 bp) sesuai dengan produk polymerase chain reaction (PCR) dari referensi dan database GeneBank. Disimpulkan bahwa dengan menggunakan metode PCR dapat terdeteksi adanya gen tst pada isolat S. aureus asal susu sapi perah dan susu kambing dari Bogor.
Deskripsi Tingkah Laku Owa Jawa (Hylobates moloch Audebert) di Taman Margasatwa Ragunan Prasetyo, Budi; Amelia, Santi
SAINSMAT Vol 2, No 2 (2013): September
Publisher : SAINSMAT

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Keberadaan Hylobates moloch (Owa Jawa) endemik di hutan-hutan konservasi seperti Taman Nasional Ujung Kulon, Gunung Halimun, Gunung Gede Pangrango, Cagar Alam Gunung Simpang, dan Leuweung Sancang, dan menurut IUCN Redlist status konservasi Owa tersebut termasuk dalam kategori endangered (genting). Penelitian bertujuan untuk mempelajari tingkah laku dan karakteristik Owa Jawa di Karantina Taman Margasatwa Ragunan (TMR). Metode kualitatif yang digunakan dalam penelitian adalah melakukan pengamatan langsung terhadap tingkah laku Owa Jawa dengan cara: a) deskripsi, b) ad libitum, c) focal sampling, dan d) scan sampling. Aktivitas harian hidup Owa Jawa yang mencakup makan dan minum, bergerak, beristirahat, serta bersosialisasi di Karantina TMR pada dasarnya mirip dengan perilaku Owa di habitat alamiahnya serta di Pusat Penyelamatan dan Rehabilitasi Owa Jawa (Javan Gibbon Center), Taman Nasional Gunung Gede Pangrango. Adanya kemiripan aktivitas harian hidup Owa Jawa mengindikasikan bahwa sudah selayaknya tidak ada alasan yang sangat mendasar dalam tata cara pemeliharaan maupun perawatan Owa Jawa di karantina TMR untuk tidak berhasil, sebaliknya harus berhasil dan berkembang menjadi lebih banyak.Kata kunci: Owa Jawa, tingkah laku, Taman Margasatwa Ragunan
ANALISIS KUALITAS PELAYANAN TERHADAP TINGKAT KEPUASAN MAHASISWA DI STIE ”ARTHA BODHI ISWARA” SURABAYA Prasetyo, Budi
DIE Vol 6, No 3 (2010)
Publisher : DIE

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In this research of writer use method either through qualitative and also quantitatively with examination of reliabilitas and validity, test doubled regresi, correlation coefficient test, parsial correlation coefficient test, test F test and tAs for result of this research is : For the examination of by parsial known by variable (tangibles) (X1), assess t calculate which obtained is equal to 9,547 and its value is 0,000, (reliability) (X2), assess t calculate which obtained[is equal to 10,245 and its value is 0,000, ( responsiveness) (X3), assess t calculate which  obtained is equal to 8,576 and its value is 0,001, (assurance) (X4), assess t calculate which  obtained is equal to 7,568 and its value [is] 0,002, (empathy) (X5), assess t calculate which  obtained is equal to 6,125 and its value is 0,000, this smaller value than = 0,05, so that can be said that that free variable (tangibles) (X1), (reliability)(X2), (responsiveness)(X3), (assurance) (X4), (empathy) (X5) by parsial have an effect on to storey; level satisfaction of student (Y)Result of calculation Test conducted F constructively SPSS program obtained [by] f value [count/calculate] equal to 980,365. obtained Signifikansi value [is] equal to 0,000, this smaller signifikansi value than value that is 0,05. Thereby can be interpreted that free variable which cover physical evidence ( tangibles) ( X1), mainstay ( reliability) ( X2), comments (responsiveness) (X3), (assurance) (X4), (empathy) (X5), together have an effect on to storey; level satisfaction of student ( Y) Because X2 variable that is (reliability) have highest parsial correlation value that is equal to 0,988 compared to other free variable hence X2 variable that is (reliability) represent dominant free variable influence variable tied that is storey; level satisfaction of student ( Y)Keywords : Quality of Service and Satisfaction
IMPLEMENTASI TUGAS DAN WEWENANG PENYIDIK TERHADAP PERLINDUNGAN PENYU HIJAU Prasetyo, Budi
Jurnal Magister Hukum Udayana (Udayana Master Law Journal) Vol 3, No 3 (2014)
Publisher : Udayana University

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The research of officer task and authority implementation towards green turtle conservation (study case of Marine Police Directorate Regional Bali) aims to describing and analizing officer task and authority implementation in deep based on both KUHAP or Conservation Law. Besides, this research also aims to figure out the enabling and inhibiting factors in implementing officer task and authority towards green turtle conservation, also researching the steps taken by of Marine Police Directorate Regional Bali. The need for green turtle in Bali is rising up to this time. Although many criminal offensers got caught by of Marine Police Directorate Regional Bali officers but it is still going on. Based on this situation, a question arises how is the implementation of officer task and authority towards green turtle conservation and what are the enabling and inhibiting factors. The method used in this research is empirical legal research with descriptive research using prime and secondary data resouces with interview and also related available documents. This research uses non probability sampling technique which is purpose sampling with quota sampling that is a process of sample withdrawing by observing the easiest sample to take and the sample has special features that attracts the researcher. With all collected data, managed using qualitative analysis and the data presented descriptively and sistematically. Based on the research done before, it is noticed that the implementation of officer task and authority towards green turtle conservation based on KUHAP and Conservation Law has been going well. Granting rights for the suspects of green turtle trading during the investigation has been done by the officers based on KUHAP. The factors which becomes the enabling task for officer task and authority towards green turtle is the law factor, law enforcement, equipment and facilities, and culture. Meanwhile the society factor is the main inhibiting factor for implementing officer task and authority towards green turtle conservation. The habit of consuming turtle meat and guise of religious ceremony are mostly used as the reasons in trading this protected animal.
KOMUNIKASI ANTARPRIBADI DAN PERUBAHAN SIKAP NARAPIDANA (Studi Deskriptif Kualitatif Mengenai Komunikasi Antarpribadi Petugas Lembaga Pemasyarakatan Dalam Merubah Sikap Narapidana Di Cabang Rutan Aceh Singkil) Prasetyo, Budi
FLOW Vol 2, No 7 (2013): Jurnal Ilmu Komunikasi FLOW
Publisher : FLOW

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Penelitian ini berjudul Komunikasi Antarpribadi dan Perubahan Sikap Narapidana, studi diskriptif kualitatif mengenai komunikasi antarpribadi petugas lembaga pemasyarakatan dalam merubah sikap narapidana di cabang Rutan Aceh Singkil, tujuanya adalah untuk mengetahui proses dan peranan komunikasi antarpribadi yang dilakukan petugas dalam merubah sikap narapidana di cabang Rutan Aceh Singkil. Objek penelitian ini adalah komunikasi antarpribadi petugas dan narapidana yang terdapat di cabang Rutan Aceh Singkil. Sedangkan yang menjadi informan yaitu dua orang petugas dan dua narapidana, di penelitian ini menggunakan Teori komunikasi, komunikasi antarpribadi, Self Disclosure, sikap dan narapidana. Teknik pengumpulan data menggunakan wawancara mendalam, observasi dan studi literatur. Sedangkan teknik analisis data menggunakan model interaktif dengan menggunakan empat tahapan seperti : koleksi, penyederhanaan data, penyajian data, dan pengambilan serta verifikasi. Hasil penelitian menunjukan bahwa, komunikasi antarpribadi sangat berpengaruh dalam proses pembinaan yang dilakukan oleh petugas terhadap narapidana. Bentuk komunikasi yang terdapat dalam pembinaan seperti komunikasi antarpribadi sesama petugas dan narapidana. Komunikasi menjadi sebuah kebutuhan yang diperlukan oleh para narapidana dalam menjalani masa hukuman, dimana sangat penting dalam bentuk komunikasi itu sendiri.   Kata kunci: komunkasi antarpribadi, perubahan sikap.
PENGATURAN TINDAK PIDANA PENCABULAN SEJENIS BERDASARKAN HUKUM PIDANA INDONESIA Prasetyo, Budi; Effendi, E; Diana, Ledy
Jurnal Online Mahasiswa (JOM) Bidang Ilmu Hukum Vol 2, No 2 (2015): Wisuda Oktober 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Ilmu Hukum

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In the Code of Criminal Indonesia have related settings of sexual crimes including sexual abuse offense. However, in the case of criminal acts of sexual abuse is often a kind of law enforcement officials to use a particular article in the Criminal Code criminalize same-sex obscene acts by adults against minors. The issue is important to note because it will affect the future of law enforcement. Existing provisions in the Criminal Code has not been specifically regulates the crime of sexual abuse of the kind, so very unfortunate to the perpetrators can not be in meshes with applicable law. The aim of this study is the first, knowing settings similar criminal acts of sexual abuse by the Indonesian Penal Both know whether the Indonesian criminal law rules have become effective to trap similar criminal sexual abuse.From these results it can be concluded that the First, arrangements similar criminal acts of sexual abuse by positive law which is regulated in Article 292 of the Criminal Code and Law No. 23 of 2002 on Child Protection. Settings on the kind of abuse in the new criminal law touching on aspects of child protection as victims and not criminalize a type of abuse committed by their fellow adults (homosexual), so that those can not be subject to legal sanctions if anyone feels aggrieved as a result of the acts of sexual abuse Second, the Criminal Code and the Child Protection Act and not running optimally effective because of the higher and increasing acts of sexual abuse against children today. Sanctions in the rules of criminal law concerning sexual abuse are often imposed on similar offenders who on average have not shown the spirit of consistent law enforcement. Suggestions of Author, First. It is expected that the legislators, especially the House of Representatives to create consistent rules of criminal law and capable of achieving justice for society and immediate improvements to the Indonesian criminal law rules terkhsusus rules relating to sexual crimes committed by those who are the same sex so that law and order can be created in accordance with the aspired according to the values of justice, benefits and actual certainty Both of the sanctions in the rules of criminal law concerning sexual abuse should be applied similar maximum penalty by law enforcement officials, especially judges in a verdict against the accused so as to provide a deterrent for the offender as well as preventive measures that certain criminals do not commit a crimeKeywords: A kind of abuse, Criminal Law Indonesia
ANALISA INDEKS KEPUASAN PADA UPT PERPUSTAKAAN UNIVERSITAS TULUNGAGUNG Prasetyo, Budi
Jurnal BONOROWO Vol 1, No 1 (2013)
Publisher : UPT Perpustakaan Universitas Tulungagung

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Dua hal penting yang merupakan fokus dari perpustakaan modern dewasa ini yaitu kepuasan pengguna dan pemanfaatan teknologi informasi untuk meningkatkan kinerja perpustakaan. Perpustakaan diharapkan mampu berperan aktif dalam menunjang kegiatan pendidikan dan pengajaran dengan cara mengumpulkan, mengolah, menyimpan, menyajikan dan menyebarluaskan informasi berupa bahan cetak maupun non cetak untuk pengguna ( civitas akademika ) sesuai dengan kebutuhannya. Layanan perpustakaan Tulungagung kepada penggunanya diukurIndeks Kepuasan Pengguna bernilai 66,4205 dengan kategori tingkat layanan adalah baik (B). Hal ini berarti bahwa layanan yang selama ini diberikan oleh perpustakaan telah memberikan kepuasan kepada pengguna perpustakaan. Unsur layanan yang paling memuaskan bagi para pengguna adalah unsur kemampuan petugas layanan (U9) dengan nilai 3,2. Unsur layanan ini telah memberikan kontribusi yang cukup signifikan terhadap tingkat kepuasan pengguna layanan pada perpustakaan Tulungagung. Unsur layanan yang menduduki nilai terendah adalah unsur kelengkapan koleksi(U7) dengan nilai 2,2, unsur keakuratan teknologi informasi (TI) (U6) dengan nilai 2.