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Pengaruh Polymorphisme PvuII Gen Reseptor Estrogen Alfa terhadap Kadar Kalsium dan Phosphat Serum pada Wanita Postmenopause Prasetyastuti, Prasetyastuti
Berita Kedokteran Masyarakat (BKM) Vol 26, No 1 (2010)
Publisher : Berita Kedokteran Masyarakat (BKM)

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Abstract

Background: Estrogen has an important role in bone metabolism. The hypoestrogenemia condition occurring in postmenopausal women may affect bone metabolism. The mechanism action of estrogen to prevent the activity of osteoclast in bone resorption was mediated especially by estrogen receptor alpha gene (ESR1). The PvuII polymorphism that occurs in ESR1 gene may alter their expression and  function.Objectives: the aim of this research was to determine the difference of serum calcium and phosphate level in postmenopausal women with PvuII polymorphism of the Estrogen receptor alpha geneMethod: This was cross sectional design. The subjects are postmenopausal women 50-70 year old .Twenty seven subject divided into two group, group I consisted of 15 women with genotype PP,Pp and group II consisted of 12 with genotype pp. polymorphism were assessed by Polymerase Chain Reaction Restriction Fragmen Length Polymorphism(PCR-RFLP) technique. Determination of serum calcium and phosphate level used spectrophotometer. The data was analyzed with Independent sample t-test.Results: The serum calcium level are 8,15 ± 1,35 mg/dl and 8,13 ± 1,58 mg/dl in genotype PP,Pp and pp respectively. The serum phosphate level are 3,8 ± 0,47 mg/dl and 4,24 ± 0,86 mg/dl in genotype PP,Pp and pp respectivelyConclusion: there were no statistically significant differences in serum calcium and phosphate level among genotype PP,Pp with pp  ESRI gene(p>0,05) PvuII polymorphism of ESRI gene did not influence serum calcium and phosphate level in postmenopausal women.Keywords: postmenopausal women, estrogen receptor polymorphism, serum calcium, serum phosphate
Korelasi Antara Kadar Vitamin C dengan Kadar Methemoglobin Darah Lansia di Kecamatan Pakem Kabupaten Sleman Prasetyastuti, Prasetyastuti
Berita Kedokteran Masyarakat (BKM) Vol 25, No 1 (2009)
Publisher : Berita Kedokteran Masyarakat (BKM)

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Abstract

Background: Oxidative damage  by  free  radical can  cause  aging  process. Erythrocyte  as an  oxygen –  carrying agent  has high  risk  of  oxidative  injury which can  inducemethemoglobin  production.  Vitamin C  is  an  exogen antioxidant  that work  in  aqueous  phase  like  blood  and  can  react   with    free  radical before  they  initiate  the oxidative  injury.Objectives: The  aim of  this study was  to  find out whether  there  is possibility  of correlation  between  vitamin C and methemoglobin  level  in blood of elderly  living  in Pakem DistrictMethod:  This was  an observational  research with  cross  sectional  design,  in which  32  elderly  from  Pakem District were  subjects.  The  blood  sample was  taken  from  cubital  vein.  Determination  of   vitamin C  and methemoglobin  level  used  spectrophotometer. Pearson Correlation was  employed  to  analyze  the  data.Results: The vitamin C and methemoglobin level of the elderly 0,43 + 0,25 mg/dl  and  0,035 +  0.02% respectively. The  correlation between  vitamin C  and methemoglobin  level was  not significant    (  p =  0.771).Conclusion: The  vitamin C was not  correlated with  the methemoglobin  level.Keywords: vitamin C, methemoglobin,  elderly,  free  radica
Vitamin E and MDA Concentrations in Plasma of Healthy Young Adult, Elderly and Pregnancy Asjari, Sri Rahajoe; Prasetyastuti, Prasetyastuti; N.A., Zainal Arifin; Ngadikun, Ngadikun; Manampiring, Aalda Ellen; Sudarmo, Risanto Siswo; Sofoewan, Sulchan
Indonesian Food and Nutrition Progress Vol 10, No 2 (2003)
Publisher : Indonesian Association of Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jifnp.104

Abstract

Vitamin E is one of essential micronutrients. It is an antioxidant which potentially protects bioinolecules against oxidative damage. One ofparameters ofoxidafive damage is MDA or lipid peroxide. In this study, concentrations of plasma vitamin E as an antioxidant and MDA as one of oxidative damage markers were measured. The subjects consisted offive groups: (I) male medical students (young adult), (II) female medical students (young adult), (III) nursery inhabitants in Yogyakarta province (Abiyoso), (IV) nursery inhabitants in Manado, North Sulawesi Province (Senja Cerah), as the old ages, and (V) pregnant women within third trimester (of Prenatal Care Unit in Sardjito Hospital). The concentrations of Vitamin E (mg/dl) and MDA (nmol/ml) were as follows: Young Adult Male (n= 28) 20,8 + 3,8 and 0,190 + 0,057 ; Young adult Female (n-28) 21,9 + 4,5 and 0,123 + 0,057 ; Old age Yogyakarta (n=41) 30,6 + 10,4 and 0,291 + 0,062 ; Old age Manado (n=40) 26,5 + 4,5 and 0,391 + 0,081 ; Pregnant Women (=28) 39,9 + 11,3 and 0,165 -f 0,067. Groups of people who were hyperlipemic tend to have high concentration of Vitamin E, while group IV who eat less vegetable protein and more PUFA tend to have higher MDA concentration.
Hubungan Dosis Tepung Gembili (Dioscorea esculenta) Dengan Tingkat Ekspresi Enzim Ampk-α2 pada Model Tikus Diabetes Melitus oktiyani, neni; Sunarti, sunarti; Prasetyastuti, Prasetyastuti; Cahyono, Jujuk Anton
Medical Laboratory Technology Journal Vol 1, No 1 (2015): periode Juni
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (995.684 KB) | DOI: 10.31964/mltj.v1i1.4

Abstract

Alternative methods of controlling glucose levels in patients with diabetes is by type of food, either by utilizing yam flour. At the flour contained inulin and resistant starch that can activate the enzyme AMPK-α2. Activation of these enzymes will stimulate glucose transport in skeletal muscle and liver, thus causing a decrease in glucose production. Varying doses of flour is expected to affect the expression of AMPK-α2. This study aims to dosage relationship yam flour (Dioscorea esculenta) with tigkat-α2 AMPK enzyme expression in the nucleus skeletal muscle and liver in mouse models of diabetes mellitus. The study was a quasi-experimental design with Post Test Only Group Design. Rats were divided into 5 (five) groups, healthy mice, the mice with type 2 diabetes, and type 2 groups of diabetic rats with dietary intake yam flour addition of 1.25 g (TG-1:25),), 2.5 g (TG -2.5), 5.0 g (TG-5.0). Yam flour is mixed into the rat diet feed with varying doses. The results showed no significant correlation between the dose of yam flour with AMPK-α2 expression levels in skeletal muscle nuclei (p = 0.312) and liver (p = 0.474) in a mouse model of DM. The need for other studies using other types of food as an alternative arrangement of food for patients with diabetes
Effect of 7-Hydroxy-2-(4- Hydroxy -3-Methoxyphenyl)-Chroman-4-one On Level of Mangan-Superoxide Dismutase (Mn-Sod) and Superoxide Dismutase 2 (Sod2) Gene Expression in Hyperlipidemia Rats Ayunda, Rahmah Dara; Prasetyastuti, Prasetyastuti; Hastuti, Pramudji
Indonesian Journal of Pharmacy Vol 30 No 3, 2019
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm30iss3pp180

Abstract

Hyperlipidemia is a lipid metabolism disorder characterized by an increase in serum lipid levels. Hyperlipidemia is a major risk factor for many metabolic syndrome diseases because it triggers oxidative stress. Oxidative stress can be reduced by endogenous antioxidant enzymes triggered by exogenous antioxidant compounds, such as 7-OH-2- (4-OH-3-methoxyphenyl)-chroman-4-one isolated from the seeds of Swietenia macrophylla King. The aims of this study were to investigate the effects of 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one compounds on cholesterol level, LDL level, Mn-SOD levels and SOD2 gene expression of hyperlipidemic rats. Thirty rats (Rattus norvegicus ) were divided into 6 groups, normal group (N), hyperlipidemia group (HL), hyperlipidemia group with simvastatin (P), hyperlipidemic group with 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one with dose 10 (F10), 30 (F30) and 90 (F90) mg/200g body weight (BW). Cholesterol and LDL were analyzed with CHOD-PAP method, Mn-SOD level was analyzed by ELISA method and SOD2 gene expression was analyzed by qPCR method. The decrease in cholesterol and LDL levels were most prevalent in group F90 with dose 90 mg/200g BW of 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one, with average difference each of them was 172.43 mg/dL and 36.12 mg/dL. The rats fed on high-cholesterol diet exhibited a significant elevation in Mn-SOD levels (p<0.05) compared to normal group. The treated animals with 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one has the level of Mn-SOD is significantly lower (p<0.05) compared with hyperlipidemic group. Expression of SOD2 in group F90 has value close to normal group (p> 0.05). 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one with dose of 90 mg/200g BW improved cholesterol levels, LDL levels, Mn-SOD levels and SOD2 gene expression in hyperlipidemic rats.