TRI PRARTONO
Marine Sciences Department, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Bogor, Indonesia Jln. Agatis, Kampus IPB Dramaga Bogor 16680 Indonesia

Published : 31 Documents
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Growth and Extracellular Carbonic Anhydrase Activity of Zooxanthellae Symbiodinium sp. in Response of Zinc Enrichment KARIM, WIDIASTUTI; KASWADJI, RICHARDUS; PRARTONO, TRI; PANGGABEAN, LILY MARIA GORETTI
HAYATI Journal of Biosciences Vol 18, No 4 (2011): December 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (76.619 KB) | DOI: 10.4308/hjb.18.4.157

Abstract

Coral reef communities contain a wide variety of mutualistic associations none more important than the relationship between corals and their symbiotic dinoflagellates of the genus Symbiodinium sp., commonly referred to as zooxanthellae. The function of Zinc (Zn) as cofactor of several enzyme systems such as extracellular carbonic anhydrase (extracellular CA) which catalyzes the interconversion of HCO3- and CO2. Concentrations of dissolved Zn in oligothropic waters are often very low therefore may limit the growth of zooxanthellae and their ability to fix CO2 from seawater via the carbonic anhydrase. The aim of this research is to investigate the effect of various concentrations of Zn on the growth and extracellular CA activity in zooxanthellae. Cell density was monitored daily by enumeration with hemocytometer-type chamber (0.1 mm). Extracellular CA was measured in homogenized intact whole cell by a pH drift assay. Results revealed that Zn status strongly influences the growth rate and extracelullar CA activity in zooxanthellae. The specific growth rate and cell density increased two-fold whilst extracelullar CA activity increased 10.5 times higher than that in control with increasing concentrations of Zn from 0 to 80 nM, but decreased when Zn was over 80 nM. Under a concentration of 80 nM was not Zn limited culture, consequently the growth rate of zooxanthellae not dependent on CO2 concentration yet offset by extracelullar CA activity.
Fatty Acid Content of Indonesian Aquatic Microalgae PRARTONO, TRI; KAWAROE, MUJIZAT; SARI, DAHLIA WULAN; AUGUSTINE, DINA
HAYATI Journal of Biosciences Vol 17, No 4 (2010): December 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (68.788 KB) | DOI: 10.4308/hjb.17.4.196

Abstract

High utilization of fossil fuel increases the level of carbon dioxide in the atmosphere and results in global warming phenomenon. These things establish the world’s thought to look for the other alternative energy that can reduce the use of fossil fuel even to be replaced by the substitute. Recently, Indonesia has been doing the research of microalgae as a feedstock of an alternative biofuel. Fatty acid content that microalgae have is also high to produce biofuel. The steps used in this research is a 7 days cultivation, harvesting, extraction using hexane, and fatty acid identification using Gas Chromatography of microalgae species. Fatty acid component in some species such as Chlorella sp., Scenedesmus sp., Nannochloropsis sp., and Isochrysis sp. is between 0.21-29.5%; 0.11-25.16%; 0.30-42.32%; 2.06-37.63%, respectively, based on dry weight calculation. The high content of fatty acid in some species of microalgae showed the potential to be the feedstock of producing biofuel in overcoming the limited utilization from petroleum (fossil fuel) presently.
Specific Growth Rate of Chlorella sp. And Dunaliella sp. According to Different Concentration of Nutrient and Photoperiod Kawaroe, Mujizat; Prartono, Tri; Sunuddin, Adriani; Wulan Sari, Dahlia; Augustine, Dina
Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Vol 16, No 1 (2009): Juni 2009
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Light and nutrient are factors that support the microalgae growth rate besides COB2B, temperature, and salinity. Microalgae growth of Chorella sp. and Dunaliella sp. were observed to determine the influences of different nutrient concentration and photo period. Microalgae cultivation was located at laboratory using 100 mL Erlenmeyer. The specific growth rate of microalgae was observed for different nutrient concentration and photo period of light treatments. Using Guillard/f2 nutrient, the highest specific growth rate for Chorella sp. was 0.227/d and 0.289/d for Dunaliella sp. The highest microalgae specific growth rate influenced different photo period was 0.39/d and 0.329/d, respectively. Finally, the highest specific growth rate for both cultivated species of microalgae was observed at 2V nutrient concentration and 24 hour period of light treatment.Keywords : Spesific growth rate, photoperiod, nutrient, Chlorella sp., Dunaliella sp.
PLUMBUM (Pb) CONCENTRATION IN ANNUAL BANDS OF CORAL Porites lutea AT TUNDA ISLAND, BANTEN Riska, .; Zamani, Neviaty P.; Prartono, Tri; Arman, Ali
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol 7, No 1 (2015): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Jurnal Ilmu dan Teknologi Kelautan Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Coral is one of marine organisms that can accumulate heavy metals such as Pb in its skeleton. The purpose of this study was to determine the concentration of heavy metals Pb in growth bands of coral P. lutea at windward and leeward regions of Tunda Island. The results showed that P. lutea accumulated heavy metals Pb in their growth bands. The results of Pb concentrations in windward region were in range of 6.17-14.76 mg kg-1/year, with the average concentration rate of 9.69 mg kg-1/year.  Meanwhile, in leeward region, the Pb concentrations were in range of 8.37-17.66 mg kg-1/year whith the average concentration rate of 13.33 mg kg-1/year. The Pb concentration in leeward regions was higher than in the windward region. Keywords: heavy metal Pb, coral P. lutea, Tunda Island
Relationship Between Light Intensity and Abundance of Dinoflagellate in Samalona Island, Makassar (Keterkaitan Intensitas Cahaya dan Kelimpahan Dinoflagellate di Pulau Samalona, Makassar) Tasak, Albida Rante; Kawaroe, Mujizat; Prartono, Tri
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 20, No 2 (2015): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (237.425 KB)

Abstract

Cahaya merupakan salah satu faktor penting dalam proses fotosintesis dinoflagellate dan pertumbuhan variabilitas harian. Intensitas cahaya memengaruhi aktivitas fotosintesis dan kelimpahan dinoflagellate. Studi ini bertujuan untuk menunjukkan pola kecenderungan kelimpahan dinoflagellate dan klorofil serta lama penyinaran terhadap kelimpahan dinoflagellate di Perairan Pulau Samalona. Penelitian dilakukan dengan menginkubasi sampel dinoflagellate kedalam botol sejak pagi-sore hari dengan inkubasi waktu pengamatan setiap 2 jam dengan ulangan sebanyak 3 kali. Pengambilan mencakup kelimpahan dinoflagellate, nutrient dan intensitas cahaya dalam perairan. Analisis data menggunakan regresi linear sederhana. Hasil penelitian menunjukkan bahwa nilai intensitas cahaya berkisar antara 50–3000 lux; kelimpahan dinoflagellate berkisar antara 9–1105 sel.L-1, dan kandungan klorofil a lebih dominan dengan kisaran  0.00069–0.50321 µg.L-1. Intensitas cahaya mempengaruhi kelimpahan dinoflagellate, namun pengaruh kandungan nutrient sangat kecil terhadap kelimpahan dinoflagellate. Pola kelimpahan dinoflagellate bervariasi dari pagi hingga sore hari yang dipengaruhi oleh intensitas cahaya dalam melakukan proses fotosintesis serta kondisi lingkungan lain seperti klorofil a dan nutrient. Hasil penelitian ini memberikan informasi variasi temporal harian kelimpahan dinoflagellate di Pulau Samalona, Makassar.
The Efficacy of Bioaugmentation on Remediating Oil Contaminated Sandy Beach Using Mesocosm Approach (Efikasi Tehnik Bioaugmentasi dalam Memulihkan Pantai Berpasir Tercemar Minyak Menggunakan Pendekatan Mesokosm) Darmayati, Yeti; Sanusi, Harpasis S.; Prartono, Tri; Santosa, Dwi Andreas; Ruyitno, Ruyitno
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 20, No 3 (2015): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (234.012 KB) | DOI: 10.14710/ik.ijms.20.3.143-152

Abstract

Bioremediation is basically consists of two approaches, biostimulation and bioaugmentation. The efficacy of bioaugmentation for combating oil pollution in field application is still argued. The purpose of study was to evaluate the efficacy of bioaugmentation and to compare the affectivity of single strain and consortium application in remediating oil polluted sandy beach. Experimental study in a field has been conducted with two (2) treatments and one (1) control in three different plots. The treatmens were introduction of a single strain (Alcanivorax sp TE-9) and a consortium (Alcanivorax sp. TE-9, Pseudomonas balearica st 101 and RCO/B/08-015) cultures into oil contaminated sediment. The experiment in mesocosm approach was taken place in Cilacap coast. Arabian light crude oil was used in the concentration of 100.000 mg.kg-1 sediment. Changes of oil concentration, bacterial density and pore water quality have been monitored periodically for 3 months. The result showed that oil degradation percentage and bacterial growth in both treatments were higher than in control. After 3 months, the percentage of oil degradation experiment in control, single strain and formulated consortium treatments were observed at 60.4%, 74.5% and 73.5%. It proves that bioaugmentation tehnique can enhance significantly oil biodegradation in sandy beach. The applications of bacteria in single or consortium culture give no different impact on their affectivity for bioremediation in Cilacap sandy beach. By data extrapolation it can be predicted that both of treatments able to reduce remediation time from 210 days into 135–137 days. Bioaugmentation can be proposed as a good solution for finalizing oil removing in Cilacap sandy beach when oil spilled occurred in this environment. Keywords: Bioremediation, bioaugmentation, oil, sandy beach, Alcanivorax, mesocosm, Cilacap   Bioremediasi pada dasarnya terdiri dari dua pendekatan yaitu biostimulasi dan bioaugmentasi. Teknik bioaugmentasi  dalam menanggulangi pencemaran minyak di lapangan masih diperdebatkan efektivitasnya. Penelitian ini bertujuan untuk mengevaluasi efikasi tehnik bioaugmentasi serta membandingkan efektivitas kultur tunggal dan konsorsium  dalam memulihkan pantai berpasir tercemar minyak. Studi eksperimental di lapangan telah dilakukan dengan menggunakan dua perlakuan dan satu kontrol di tiga plot berbeda, Perlakuannya adalah penambahan bakteri kultur tunggal (Alcanivorax sp TE-9) dan bakteri konsorsium (Alcanivorax sp. TE-9, Pseudomonas balearica st 101 dan RCO/B/08-015) ke dalam sedimen yang tercemar minyak. Eksperimen dengan pendekatan mesokosm dilakukan di pantai Cilacap. Minyak mentah ringan Arabia dengan konsentrasi 100.000 mg.kg-1 sedimen digunakan sebagai bahan cemaran. Perubahan konsentrasi minyak, kepadatan bakteri dan parameter lingkungan diamati secara periodik selama 3 bulan percobaan. Hasil penelitian menunjukkan bahwa persentase degradasi minyak dan densitas bakteri di sedimen perlakuan lebih tinggi daripada kontrolnya. Setelah 3 bulan eksperimen, persentase degradasi minyak pada kontrol, perlakuan kultur tunggal dan konsorsium masing-masing teramati 60.4%, 74.5% and 73.5%. Hal ini membuktikan bahwa tehnik bioaugmentasi secara signifikan mampu meningkatkan biodegradasi minyak di pantai berpasir. Pemberian mikroba dalam bentuk kultur tunggal ataupun konsorsium mempunyai efektivitas yang tidak berbeda untuk proses bioremediasi di pantai berpasir Cilacap.  Dengan ekstrapolasi data dapat diduga bahwa kedua perlakuan bioaugmentasi ini mampu mempercepat waktu pemulihan lingkungan dari 210 hari ke 135-137 hari. Bioaugmentasi dapat diusulkan sebagai solusi yang cukup baik untuk menghilangkan minyak pada tahap akhir pembersihan pantai berpasir Cilacap, jika terjadi tumpahan minyak di lingkungan ini. Kata kunci: single strain, consortium, bioaugmentation, oil, bioremediation, sandy beach,  Alcanivorax, Cilacap
Toxicity of Ammonia to Benthic Amphipod Grandidierella bonnieroides: Potential as Confounding Factor in Sediment Bioasssy (Toksisitas ammonia terhadap amphipod bentik Grandidierella bonnieroides : Potensi sebagai faktor pengganggu dalam bioassay sedimen) Hindarti, Dwi; Arifin, Zainal; Prartono, Tri; Sanusi, Harpasis S.
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 20, No 4 (2015): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (324.696 KB) | DOI: 10.14710/ik.ijms.20.4.215-222

Abstract

Toxicity of ammonia was evaluated using amphipod Grandidierella bonnieroides to describe its role as confounding factor in sediment quality assessment. Ammonia is a toxic compound that is found naturally in seawater and sediment. High ammonia content in the pore water sediment can be potentially toxic to benthic biota, so that it will interfere with the results of sediment toxicity tests. Laboratory production amphipod was used in this ammonia toxicity test. Water-only toxicity tests was conducted to produce new toxicity data of ammonia, and is expressed as LC50, LOEC and NOEC for benthic amphipod G.bonnieroides. The study resulted the 96-h median lethal concentration (LC50) of ammonia for G. bonnioerides was 65.5 mg.L-1. While the value LOEC (low observed effect concentration) is 56 mg.L-1 and NOEC value (no observed effect concentration) was 32 mg.L-1 . This shows that ammonia has a relatively low toxicity to amphipod and ammonia does not act as a confounding factor in the sediment toxicity test, because the ammonia content in sediment does not pose any significant effect on amphipod survival. It can be concluded that the amphipod has a potential useful as test organism in sediment bioassay for assessing the quality of marine sediment. Moreover, the high dependence of total ammonia toxicity indicates that it is necessary to measure the total ammonia and pH of the medium when testing environmental samples. Keywords: ammonia, amphipod, toxicity, confounding factor, bioasssay Toksisitas ammonia dievaluasi menggunakan amphipod Grandidierella bonnieroides untuk menggambarkan perannya sebagai faktor pengganggu dalam penilaian kualitas sedimen. Amonia merupakan senyawa beracun yang ditemukan secara alami dalam air laut dan sedimen. Kandungan amonia yang tinggi dalam air pori sedimen dapat berpotensi racun bagi biota bentik, sehingga akan mengganggu hasil uji toksisitas sedimen. Amphipod hasil produksi laboratorium digunakan dalam uji amonia. Uji toksisitas dilakukan untuk menghasilkan data toksisitas baru ammonia, dan dinyatakan sebagai LC50, LOEC dan NOEC untuk G.bonnieroides amphipod bentik. Studi ini menghasilkan nilai konsentrasi median letal 96-jam (LC50) ammonia untuk G. bonnioerides adalah 65.5 mg.L-1. Sedangkan nilai LOEC (konsentrasi terendah yang menyebabkan dampak secara signifikan) adalah 56 mg.L-1 dan nilai NOEC (konsentrasi tertinggi yang tidah menyebabkan dampak secara signifikan) 32 mg.L-1. Hal ini menunjukkan bahwa ammonia memiliki toksisitas relatif rendah untuk amphipod dan ammonia tidak bertindak sebagai pengganggu dalam untuk uji toksisitas sedimen, karena kandungan ammonia dalam sedimen tidak menimbulkan pengaruh yang nyata pada survival amphipod. Dapat disimpulkan bahwa amphipod memiliki potensi sebagai biota uji dalam sedimen bioassay untuk menilai kualitas sedimen laut. Selain itu, terdapat ketergantungan yang tinggi toksisitas amoniak terhadap pH, sehingga perlu untuk memantau total amonia dan pH saat melakukan pengujian sampel lingkungan. Kata kunci : ammonia, amphipod, toksisitas, faktor pengganggu, bioassay
Oil Spill Biodegradation by Bacteria Isolated from Jakarta Bay Marine Sediments Dwinovantyo, Angga; Prartono, Tri; Rahmaniar, Restya; Prabowo, Nico Wantona; Susanti, Santi
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 1 (2016): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.69 KB) | DOI: 10.14710/ik.ijms.21.1.29-36

Abstract

A laboratory study was conducted with the aim to isolate and identify bacteria from sea sediment and test their biodegradation ability in two place where contaminated with oil spill. Five sediment samples were dissolved by using sterile sea water, and then bacteria isolated with total plate count (TPC) method. Isolates bacteria was cultivated, and adapted using the nutrient conditioned sea water medium. Biodegradation process was done by mixing the bacteria with crude oil and shaken for few days. The number of bacteria isolated varied from 2 x 102 CFU ml-1 to 6 x 106 CFU ml-1 and apparently increased after cultivation and adaptation with oily media. Bacteria identified during this study were Fundibacter sp., Alcanivorax sp., and Marinobacter sp.. The result of biodegradation process was statistically analyzed and obtained that the bacteria are effective in degrading oil in seven days with constant of biodegradation rate was 0.1766. GC-MS analysis was conducted to prove the decomposition of carbon chain by bacteria and revealed oil degradation in carbon number 11 to 27. Based on all analysis, marine sediment bacteria can degrade the oil spill. Keywords : Bacteria, Biodegradation, GC-MS, Marine Sediments, Oil spill.
Accumulation of Heavy Metals (Cu and Pb) In Two Consumed Fishes from Musi River Estuary, South Sumatera Eka Putri, Wike Ayu; Bengen, Dietriech G; Prartono, Tri; Riani, Etty
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 1 (2016): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (474.451 KB) | DOI: 10.14710/ik.ijms.21.1.45-52

Abstract

Fish is one of the protein sources for humans which its existence is susceptible to the contamination, one of which is the heavy metal. The lack of information regarding the content of heavy metal in the edible fish in South Sumatera makes this study important to be done. This study was aimed to analyze the concentration of heavy metal in two species of edible fishes at Musi River Estuary site. The study was conducted in the estuary section of Musi River from September to November 2014. The heavy metals of Cu and Pb in the water and in the fish organs were analyzed using AAS with a type of SpektrA A-20 Variant Plus using a mixture of Air-Acetylene flame. The result showed the variation of Cu and Pb concentrations in each of species and three organs observed. The concentration of Cu and Pb in the liver was higher than in the gills and the muscle (liver>gills>muscle). The concentration of Cu and Pb in the muscle of all fish species were not exceed the safe limit for consumption. Keywords: Cu and Pb, consumed fish, Musi River estuary
Characteristics of Diatoms in Strait of Rupat Riau (Karakteristik Diatom di Selat Rupat Riau) Larasati, Chandrika Eka; Kawaroe, Mujizat; Prartono, Tri
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 20, No 4 (2015): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (431.631 KB) | DOI: 10.14710/ik.ijms.20.4.223-232

Abstract

Selat Rupat merupakan selat kecil yang berdekatan dengan Selat Malaka yang memiliki berbagai macam aktivitas antropogenik. Tekanan dari aktivitas tersebut dapat menyebabkan perubahan kondisi lingkungan perairan sehingga dapat berpengaruh pada organisme laut khususnya kelimpahan diatom. Penelitian ini bertujuan untuk menganalisis karakteristik lingkungan perairan yang memengaruhi kelimpahan diatom di permukaan Perairan Selat Rupat. Penelitian ini dilaksanakan di Selat Rupat Riau dengan 5 stasiun yang berbeda karakteristik lingkungannya pada saat pasang dan surut. Parameter yang diukur, yaitu: nitrat, fosfat, silikat, ammonia, intensitas cahaya, salinitas, Pb, minyak dan lemak, serta kelimpahan jenis diatom. Analisis komponen utama (PCA) digunakan untuk menganalisis keterkaitan parameter fisika kimia perairan dengan kelimpahan diatom. Hasil menunjukkan bahwa terdapat 11 jenis diatom yang ditemukan selama penelitian.  Kelimpahan diatom yang memiliki nilai tertinggi saat pasang dan surut adalah Coscinodiscus (10693-197160 sel.m-3 saat pasang dan 8020-186466 sel.m-3 saat surut) dan Skeletonema (40769-106266 selm-3 saat pasang dan 30744-145029 sel.m-3 saat surut). Kandungan NO3 (0.081-0.142 mg.L-1 saat pasang dan 0.090-0.235 mg.L-1 saat surut), SiO2 (0.054-0.075 mg.L-1 saat pasang dan 0.056-0.120 mg.L-1 saat surut), arus (0.3-0.5 m.det-1 saat pasang dan 0.4-0.6 m.det-1 saat surut), dan intensitas cahaya (37-113 lx saat pasang dan 37-233 lx saat surut). Parameter fisika kimia perairan tersebut, memiliki pengaruh yang besar terhadap kelimpahan diatom. Aktivitas antropogenik memengaruhi kelimpahan diatom yang berdampak pada rantai makanan di ekosistem Perairan Selat Rupat sehingga perairan tersebut perlu dikelola dengan baik agar keseimbangan ekosistem perairan tetap terjaga. Kata kunci: antropogenik, diatom, nutrien, pasang surut, Selat Rupat Rupat Strait is one of small strait in Malacca Strait, which has a wide range of anthropogenic activities. The pressure of anthropogenic activities in Rupat Strait Riau could changed the conditions of aquatic environment which was took effects on marine organisms including an abundance of diatoms. This research was aimed to analyze the factors of physic and chemical of waters that affected an abundance of diatom on surface of water in Rupat Strait Riau. This research was conducted in Rupat Strait Riau at 5 stations with different environment characteristics during high and low tide. The measured parameters, consist of nitrate, phosphate, silicate, ammonia, light intensity, salinity, Pb, oils and fats, and then the abundance of diatoms. Principal component analysis (PCA) has used to analyze relations of physic and chemical of waters with abundance of diatoms. The study found 11 genus diatoms which has the highest value at high tide and low tide is Coscinodiscus, which ranged 10.693-197.160 sel.m-3 at high tide and 8.020-186.466 sel.m-3 at low tide, and Skeletonema (40.769-106.266 sel.m-3 at high tide and 30.744-145.029 sel.m-3 at low tide). The contents of NO3 ranged among (0.081–0.142 mg.L-1 at high tide and 0.090-0.235 mgL-1 at low tide), SiO2 ranged (0.054–0.075 mg.L-1 at high tide and from 0.056–0.120 mg.L-1 at low tide, the current ranged (0.3-0.5 m.s-1 at high tide and 0.4-0.6 m.s-1 at low tide), and light intensity ranged (37-113 lx at high tide and 37-233 lx at low tide). Those parameters of physics and chemical had contributions to an abundance of diatoms, but the tidal had no big effects to an abundance of diatoms. The anthropogenic activities had the effects to an abundance of diatoms that have impact on the food chain in aquatic ecosystem at Rupat Strait, so it needs to be managed well for maintain the balancing of aquatic ecosystem.   Keywords: antropogenic, diatom, nutrient, tidal, Selat Rupat