Deni Pranowo
Chemistry Department, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Yogyakarta

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Laporan Baru: Spesies Lalat Buah Terpikat 4-(4-Hidroksi-Fenil)-2-Butanon Pranowo, Deni; Martono, Edhi; Arminuddin, Ahmad Taufiq; Suputa, Suputa
Jurnal Perlindungan Tanaman Indonesia Vol 15, No 1 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (474.268 KB) | DOI: 10.22146/jpti.11760

Abstract

Acetylation of 4-(4-hydroxy-penyl)-3-butene-2-one by anhydride of acetic acid catalyzed by sulphuric acid results in 4-(4-acetoxy-phenyl)-3-butene-2-one compound, and 4-(4-hydroxy-phenyl)-2-butanone compound as a by product. The first compound alone doesn’t attract fruit flies, but a mixture of the two products attracts male fruit flies. This mixture was tested for its attractancy to the fruit flies in Bantul, Sleman and Kulon Progo regencies, Yogyakarta Special Province; along with Cue and Methyl eugenol lures as comparison. The result showed that the mixture was able to attract four fruit flies species, i.e. Bactrocera albistrigata, B. caudata, B. cibodase, and Bactrocera sp1. One genus found has not been able to be identified to species level. Based on its wing morphometric measures, this species is put under Bactrocera dorsalis complex group. Reaksi asetilasi terhadap 4-(4-hidroksi-fenil)-3-buten-2-on dengan asam asetat anhidrid berkatalis asam sulfat menghasilkan senyawa 4-(4-asetoksi-fenil)-3-buten-2-on dan hasil samping berupa senyawa 4-(4-hidroksi-fenil)-2-butanon. Senyawa 4-(4-asetoksi-fenil)-3-buten-2-on tidakmemiliki daya pikatterhadap lalat buah, sedangkan campuran kedua senyawa hasil asetilasi tersebut memiliki daya pikat terhadap lalat buah jantan. Uji atraktansi campuran kedua senyawa ini terhadap lalat buah dilakukan di daerah Bantul, Sleman, dan Kulon Progo, Daerah Istimewa Yogyakarta dengan CUE lure dan ME lure sebagai pembanding. Hasil penelitian menunjukkan bahwa campuran senyawa 4-(4-asetoksi-fenil)-3-buten-2-on dan 4-(4-hidroksi-fenil)-2-butanon mampu memikat 4 spesieslalat buah. Empat spesies lalat buah yang terpikat tersebut adalah Bactrocera albistrigata, B. caudata, B. cibodase, dan Bactrocera sp1. Satu Genus Bactrocera tidak teridentifikasi sampai tingkat spesies. Bactrocera sp1. diduga merupakan spesies lalat buah yang belum pernah dideskripsi sebelumnya. Berdasar perhitungan wing morphometric technique, spesies lalat buah tersebut dikelompokkan ke dalam kelompok Bactrocera dorsalis Complex.
VALIDATION OF PCR-RFLP TESTING METHOD TO DETECT PORCINE CONTAMINATION IN CHICKEN NUGGET Raharjo, Tri Joko; Cahyaningtyas, Winda; Surajiman, Surajiman; Istini, Istini; Pranowo, Deni
Indonesian Journal of Chemistry Vol 12, No 3 (2012)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (428.338 KB) | DOI: 10.22146/ijc.21347

Abstract

PCR-RFLP technique to detect porcine contamination in chicken nugget has been developed and validated in this research. Various concentrations of pork were fortified during preparation of the nugget. DNA was then isolated from the nugget followed by PCR employed primers which targeted a 359 bp cytB gene fragment of mitochondrial DNA. For RFLP, the PCR product was digested by means of BamHI and BseDI enzymes. Cutting DNA fragments from nugget containing pork using BseDI enzyme produced DNA fragment with size 228 and 131 bp, while cutting with BamHI enzyme produce DNA fragments with sizes 244 and 115 bp. All of these fragments were not present in RFLP analysis of pork-free nugget. The method shows good specificity and precision and could detect porcine contamination in the nugget up to 5%. The method has been applied to test commercial nugget. Four brand of Halal-labeled commercial nugget as well as four brand of non labeled one gave negative porcine contamination.
ADSORPTION OF AFLATOXIN B1 IN CORN ON NATURAL ZEOLITE AND BENTONITE Nuryono, Nuryono; Agus, Ali; Wedhastri, Sri; Maryudhani, Y.M.S.; Pranowo, Deni; Yunianto, Yunianto; Razzazi-Fazeli, Ebrahim
Indonesian Journal of Chemistry Vol 12, No 3 (2012)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (458.697 KB) | DOI: 10.22146/ijc.21343

Abstract

A study on adsorption of AFB1 in corn (kernel and grained) on natural zeolite and bentonite has been investigated. The first work was adsorption in a batch system of standard AFB1 solution on adsorbents. Some factors such as contact time, concentration of AFB1 and particle size of adsorbent were evaluated. The amount of AFB1 adsorbed was calculated based on the difference of AFB1 concentration before and after adsorption determined by high performance liquid chromatography (HPLC) method. Adsorption of AFB1 in corn sample was emphasized by mixing aqueous suspension of sample with adsorbent. Concentration of AFB1 in suspension was analyzed by enzyme-linked immuno-sorbent assay (ELISA) method. Result shows that adsorption of AFB1 on adsorbents of natural zeolite and bentonite is very fast. Within 15 min 99% of AFB1 (200 ng/mL) has been adsorbed by 25 mg of bentonite and 96% by zeolite. The particle size higher than 200 mesh did not give significant effect on the AFB1 adsorption capability. Effectiveness of zeolite in adsorbing AFB1 is lower than that of bentonite. Capability in reducing AFB1 contamination in corn samples (kernel and meal) for both adsorbents is lower than that in standard solution.
CYTOTOXICITY IDENTIFICATION OF THE SUPERNATANT OF Manihot esculenta Crantz RHIZOME AS RIBOSOME-INACTIVATING PROTEIN TO NORMAL CELL Astuti, Endang; Matsjeh, Sabirin; Haryadi, Winarto; Pranowo, Deni; Mulatsih, Nuning Sri
Indonesian Journal of Chemistry Vol 4, No 2 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.72 KB) | DOI: 10.22146/ijc.21857

Abstract

The supernatant of Manihot esculenta Crantz rhizome has been used as an alternative remedy of infectious and cancer in Jogjakarta. Cytotoxicity assay showed that  Manihot esculenta Crantz supernatant had cytotoxic effect to cancer cell line, namely Myeloma (LC50 = 180,24 μg/mL) and HeLa (LC50 = 415,55 μg/mL), but have a little cytotoxic effect to SiHa. This research was aimed to identify cytotoxic activity of Manihot esculenta Crantzs supernatant to normal cell, particularly to human mononuclear cell and Vero cell line, and to compare to the cancer cell lines. The result showed that supernatant of M. esculenta had cytotoxic effect to normal mononuclear cell (LC50 = 564,00 μg/mL) and Vero cell line (LC50 = 686,00 μg/mL). The supernatant of M. esculenta had the highest cytotoxic activity to myeloma and relatively toxic to cervix cancer HeLa and normal cell, but less to SiHa.   Keywords: Manihot esculenta Crantz, Ribosome-inactivating Protein (RIP), cytotoxic, normal cell, cancer cell
SYNTHESIS OF 4-(3,4-DIMETOXY-PHENYL)-3-BUTENE-2-ON AND ACTIVITY IT’S TEST AS A FRUIT FLIES ATRACTANT Pranowo, Deni; Suputa, Suputa; Wahyuningsih, Tutik Dwi
Indonesian Journal of Chemistry Vol 6, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.133 KB) | DOI: 10.22146/ijc.21783

Abstract

4-(3,4-dimethoxyphenyl)-3-buten-2-on has been synthesized from veratraldehyde and acetone via aldol condensation. The reaction was performed at room temperature under basic condition for 24 hours to give brown solid of product (m.p 64-6 5oC) in 73.7% yield. Veratraldehyde itself was produced from alkylation of vanillin by the use of dimethylsulfate. The structure of the products was analyzed by FTIR, 1H NMR and GC-MS. Activity test of 4-(3,4-dimethoxyphenyl)-3-buten-2-on as an attractant was carried out in Sleman, Bantul and Kulon Progo with methyl eugenol as a reference. The result showed that 4-(3,4-dimethoxyphenyl)-3-buten-2-on was inactive compound as a fruit flies attractant and some of fruit flies, i.e. Bactrocera papayae, B. carambolae, B. umbrosa and B. abdolonginqua was found on the test area.   Keywords: 4-(3,4-dimetoxy-phenyl)-3-butene-2-on, Bactrocera spp., attractant
ANALYSIS OF FREE FATTY ACID ON SOYBEAN OIL USING GAS CHROMATOGRAPHY – MASS SPECTROSCOPY Pranowo, Deni; Muchalal, M
Indonesian Journal of Chemistry Vol 4, No 1 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (159.009 KB) | DOI: 10.22146/ijc.21875

Abstract

Research on transesterification of soybean oil with sodium methoxide as the catalyst in methanol has been conducted. Yields of transesterification reaction were analyzed by gas chromatography-mass spectroscopy (GC-MS). GC-MS spectrum showed that mehtyl palmitic, methyl stearic, methyl oleic and methyl linoleic were produced from the reaction. The reaction was done for 90 minutes and gave convertion of 84.53% (b/b).   Keywords: transesterification, soybean oil
THE INFLUENCE OF ORGANIC SOLVENT PROTEIN PRECIPITATION ON SDS PAGE PROTEIN PROFILE IN SERUM Raharjo, Tri Joko; Suprihatin, Rusmiati; Pranowo, Deni
Indonesian Journal of Chemistry Vol 7, No 3 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.948 KB) | DOI: 10.22146/ijc.21680

Abstract

A study on the influence of organic solvent protein precipitation to the profile of the serum protein has been accomplished. The expected conditions were precipitation of abundant proteins present in serum result in increasing relative concentration of minor protein which can be useful for sample preparation for biomarker studies. The serum were precipitated with various diluted (
CYTOTOXICITY OF Phaleria macrocarpa (Scheff.) Boerl. FRUIT MEAT AND SEED ETHANOL EXTRACT TO MONONUCLEAR PERIFER NORMAL CELL OF HUMAN BODY Astuti, Endang; Pranowo, Deni; Puspitasari, Santi Dwi
Indonesian Journal of Chemistry Vol 6, No 2 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.076 KB) | DOI: 10.22146/ijc.21763

Abstract

There were many research on Phaleria macrocarpa (Scheff.) Boerl. fruit for its activity as possible anticancer. However, there wasnt investigation that P. macrocarpa seed and fruit meat ethanol extract effect to normal cell. The research was conducted to identify the ethanol extract of P. macrocarpa for cytotoxic activity against mononuclear perifer normal cell of human blood. The research comprised several sections including P. macrocarpa seed and fruit meat maceration with ethanol, and the cytotoxic activity test against mononuclear normal cell. The results showed that the ethanol extract of P. macrocarpa fruit meat and seed was slightly toxic against mononuclear normal cell with the LC50 of 3817.54 μg/mL and 1349.29 μg/mL respectively. Tamoxifen and 5-fluorourasil, anticancer drugs were extremely toxic against mononuclear normal cell giving LC50 of 3.52 μg/mL and 4.05 μg/mL. The ANOVA f-test (P
Validation of a Non-Specific Dye Real-Time PCR Assay for Porcine Adulteration in Meatball Using ND5 Primer Raharjo, Tri Joko; Alfiraza, Ery Nourika; Enjelina, Esti; Pranowo, Deni
Indonesian Journal of Chemistry Vol 17, No 2 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (359.999 KB) | DOI: 10.22146/ijc.22646

Abstract

Porcine adulteration in meatball samples were analyzed using real-time polymerase chain reaction (RT-PCR), based on the ND5 primer obtained by previous study. This work consisted of three stages which were annealing temperature optimization, method validation, and application. DNA template was extracted using phenol-CIAA (chloroform-iso amyl alcohol) method. The optimum annealing temperature for ND5 primers (forward primer 5-CATTCGCCTCACTCACATTAACC-3 and reverse primer 5-AAGAGAGAGTTCTACGGTCTGTAG-3) was 58.0 °C, obtained after testing annealing at 50.5 to 59.5 °C gradient temperature with 5 °C interval. Melting curve analysis was done at 65.0 to 95.0 °C, with increasing temperature for 0.5 °C per 2 sec. Method was validated for its specificity, precision and limit of detection. RT-PCR method with ND5 primers produced 227 bp DNA fragment with 78.50 °C Tm value. From eight commercial meatball samples, one was detected containing porcine. The methods showed high specificity and precision, with experimentally determined limits for porcine were no less than 1%.