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Isolasi dan Karakterisasi Bakteri Endofit Pada Tanaman Teh (Camellia Sinensis (L.) O. Kuntze) Produktif dan Belum Menghasilkan Klon GMB 7 Dataran Tinggi

Biospecies Vol 7, No 01 (2014)
Publisher : Biospecies

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Abstract

Tujuan penelitian ini adalah untuk mengeksplorasi bakteri endofit pada tanaman tehbelum menghasilkan klon GMB 7 pada dataran tinggi di Pusat Penelitian Teh dan Kina, Gambung.Penelitian dilaksanakan di Laboratorium Mikrobiologi PPTK Gambung. Isolasi dilakukan dengancara metode sebar dan metode tuang. Sumber isolat diambil dari bagian daun, batang, dan akartanaman. Media yang digunakan adalah media TSA (Trypic Soy Agar). Pengamatan morfologimakroskopik dilakukan untuk mengamati bentuk koloni bakteri endofitik tersebut. Dari hasilpenelitian diperoleh jumlah isolat bakteri endofit pada TBM sebanyak 13 isolat, yaitu isolat padabagian daun terdapat lima isolat, empat isolat pada bagian batang dan bagian akar. Dari hasilpengamatan terdapat satu isolat dengan karakteristik yang hampir sama pada setiap jaringan(daun, batang, dan akar) tanaman. Selain itu juga terdapat isolat bakteri yang memiliki filamenfilamendi sekitar koloni yaitu isolat yang berasal dari bagian akar tanaman teh yang belummenghasilkan. Sedangkan bakteri endofit pada TM sebanyak 11 isolat, yaitu pada bagian daunterdapat tiga isolat, pada bagian batang tiga isolat dan pada bagian akar lima isolat. Dari hasilpengamatan terdapat satu isolat dengan karakteristik yang hampir sama pada setiap jaringan(daun, batang, dan akar) tanaman. Dan juga terdapat isolat bakteri yang memiliki filamen-filamendi sekitar koloni yaitu isolat yang berasal dari bagian akar tanaman teh menghasilkan, sama halnyadengan tanaman teh belum menghasilkan.

INFEKSI SALURAN KEMIH DI INSTALASI RAWAT INAP RSUD BANYUMAS PERIODE AGUSTUS 2009 – JULI 2010

PHARMACY, Jurnal Farmasi Indonesia Jurnal Pharmacy, Vol.09 No. 02 Agustus 2012
Publisher : Faculty of Pharmacy, University Muhammadiyah of Purwokerto

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Abstract

ABSTRAK Infeksi Saluran kemih ( ISK ) adalah keadaan dimana saluran perkemihan terinfeksi oleh patogen yang menyebabkan inflamasi. ISK biasa ditemukan pada masa anak anak hingga manula. Pasien ISK bergejala menerima terapi antimikroba. Ada banyak jenis antibiotika dari bermacam macam golongan dalam pengobatan ISK.  Penelitian ini dianalisis menggunakan metode retrospektif pada 115 pasien rawat inap RSUD Banyumas Periode Agustus 2009 – Juli 2010  dan dibandingkan dengan Pedoman Diagnosa dan Terapi RSUD Banyumas. Hasil menunjukan, penderita ISK adalah 12,17% pediatrik, 71,30% dewasa dan  16,52% Lansia. Sebanyak 74,78%  pasien menunjukan positif bakteriuria dan 25,12% negatif. Terdapat 12 jenis antibiotika yang digunakan dalam 115 kasus. Antibiotika Ceftriakson sebesar 59,38% merupakan antibiotika yang banyak digunakan. Kata kunci: Antibiotika, Pasien, ISK, RSUD Banyumas  ABSTRACT Urinary Tract Infection ( UTI ) is a condition in which the urinary tract is infected with a pathogen causing inflammation. UTI is commonly found on children to geriatric periods. Symptomatic UTI patient should receive an antimicrobial therapy. There is a lot of agents from many groups of antibiotic commonly used which are treat UTI.This research analized with retrospectif methods to 115 hospitalized patient at RSUD Banyumas in August 2009 – July 2010 and compared to Pedoman Diagnosa dan Terapi RSUD Banyumas. The result shows, UTI patient are 12,17% pediatric, 71,30% adult and 16,52% geriatric. In amount of 74,78%  patient show positif bacteriuria  and  25,12% negatif. There is twelve antibiotic agents are used by 115 cases. Ceftriaxone 59,38%, is poppular antibiotic.   Keywords: Antibiotic, patient, UTI, RSUD Banyumas.

Analysis of Tea Rhizosphere Bacterial Community at the Seedling Stage Using Terminal Restriction Fragment Length Polymorphism (TRFLP) Techniques

Jurnal Penelitian Teh dan Kina Vol 19, No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

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Abstract

Bio-imunizer contains an active compound of  Chryseobacterium sp. and Bacillus sp. has been developed by PPTK Gambung. This formula has positive effect on the growth of tea plants also potentially increasing resistance of the plant. The purpose of this study was to determine the effects of bacteria in Bio-imunizer to the rhizosphere bacterial communities as well as the consistency of its existence after application on tea plants at the nursery stage. The technique used in this research is Terminal Restriction Fragment Length Polymorphism based on metagenomic and culture dependent approaches. The value of relative abundance, Shannon diversity index, Pielous evenness index, and Simpson dominance index were calculated. Based on the T-RF profiles of rhizosphere bacterial communities show that Chryseobacterium sp. and Bacillus sp. which is the active compound of  Bio-imunizer consistently found in the tea plant rhizosphere. Application of Bio-imunizer can increase the diversity of rhizosphere bacterial community without affecting the communities that already exist.

Exploration and Effectiveness Test from Some Tea Indigenous Potassium Soluble Bacteria

Jurnal Penelitian Teh dan Kina Vol 19, No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

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Abstract

Tea is a plant that benefits for health. The growth of tea plant was influenced by several factors, including superior clones and nutrient availability in soil. One of the most important macro nutrients is potassium. However, the availability of potassium is low because of retention on the soil. One of the methode for absorbed by plant was used Potassium Solubilizing Bacteria (PSB) with its organic acid function. The aim of the study was to explore and determine the density of colonies (DC) and the potential of Tea Indigenous PSB. Inoculum source from tea rhizosphere and rhizoplan of GMB 7, GMB 9 and TRI 2024 clones. The experimental design used was completely randomized design with six treatments and four replications, i.e. : GMB 7 Rhizosphere; GMB 7 Rhizoplan; GMB 9 Rhizosphere; GMB 9 Rhizoplan; TRI 2024 Rhizosphere; and TRI 2024 Rhizoplan. Ten isolates with the highest halozone was tested by the Dissolution Index (DI) of the PSB. The result showed that the highest DC was GMB 9 rhizoplan by 10.9 x 105 CFU/g and the lowest was TRI 2024 rhizoplant by 3.1 x 105 CFU/g. The DC on GMB clones series showed that rhizoplan was 14,29% higher than rhizosphere. There is no significant difference of DI for PSB colonies from all clones. From that clones, we were obtained 10 isolat that have the highest halozone, that were 2 isolates from rhizosphere and 8 isolates from rhizoplan. The average of DI rhizosphere isolates was 119,9% higher than rhizoplan.

Potency of tea plant indigenous microbe on plant growth and to against blister blight disease (Exobasidium vexans Massee)

Jurnal Penelitian Teh dan Kina Vol 19, No 1 (2016)
Publisher : Research Institute for Tea and Cinchona

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Abstract

The chemical control method of blister blight (Exobasidium vexans Massee) on tea could inflict various negative impacts. In order to obtain an environmentally sound control method of blister blight disease, a nursery trial has been conducted to know the effectiveness of bacterial combinations. The trial was carried out at Gambung experimental garden, with seven treatments and four replications. The indigenous microbial codes are Azoto II-1, Endo-5 and Endo-76. The treatments tested comprised: control (without bacteria),  Azoto II-1 25% + Endo-5 75%; Azoto II-1 50% + Endo-5 50%; Azoto II-1 75% + Endo-5 25%;  Azoto II-1 25% + Endo-76 75%; Azoto II-1 50% + Endo-76 50%; and Azoto II-1 75% + Endo-76 25%. All of the treatments was applied as a soil drench, 50 ml/plant with bacterial concentration at 0,5%. The parameter observed was blister blight disease intensity, plant heights, stem diameter, leaves number, root length, and root volume. The results showed that the combination of Azoto II-1 75% + Endo-5 25% could suppress the intensity of blister blight disease with disease intensity 1.27%. The treatments also affected plant heights, stem diameter, leaves number, root length, and root volume, 15.32 cm; 3.38 cm; 8.05 cm; 18.25 cm and 2.37 cm, respectively.

The effect of indigenous and exogeneous endophythic bacteria on shoot production, soil and tea leaf nitrogen content under drought season

Jurnal Penelitian Teh dan Kina Vol 17, No 1 (2014)
Publisher : Research Institute for Tea and Cinchona

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Abstract

Drought season with rainfall <100 mm/month for more than two months cause tea plant growth disturbed, reduction in leaf pro­duction, and death of plant. Nitrogen was the macro essential nutrient needed for vegetative growth and was important for building the cell, include protein, DNA, and RNA. Nitrogen was related on proline content to support the drought tolerance in plant. The nitrogen re­quirement on tea plantation can be fulfilled by anorganic, organic, and biofertilizer. This study was aimed at determining effect of indigenous and exogeneous endophythic bacteria effec­tiveness on productivity and nitrogen content on soil and tea plant. This research was carried out at the Block A7 Gambung, Research Intitute for Tea and Cinchona. The study was conduc­ted during drought season (June to September 2013). The experimental design was randomize blocked design with factorial arrangement and three levels of each factor: i.e. 1. anorganic nitrogen dose (N): a. 100% N dose; b. 75% N dose; c. 50% N dose, 2. endophytic bacteria (E); a. control (without); b. indigenous (DtG7-5); c. exogenous (Acinetobacter sp). The result showed that the indigenous and exogenous endophytic bacteria could be maintained the leaf production and significantly different on drought season, i.e. on 3rd, 4th, and 6th plucking. The highest leaf production increasing depend on before applied the treatment was the combined of endophitic bacteria and 100% anorganic fertilizer. All of the treatment has not significantly different on N-total of soil and N content of plant. The applied of indigenous and also exogenous endophytic bacteria was pro­duced the average of nitrogen content on soil around 10-13% higher than the control treat­ment (only anorganic fertilizer applied) and the nitrogen content on plant was lower 1-2% than control treatment (only anorganic fertilizer applied).

The comparison of effectivity from consortium bio fertilizer and endophytic bio fertilizer on productivity and health of clone mature GMB 7 tea crop

Jurnal Penelitian Teh dan Kina Vol 17, No 2 (2014)
Publisher : Research Institute for Tea and Cinchona

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Abstract

This study aimed at comparing the effec­tiveness of consortium bio-fertilizer with endo­phytic bio fertilizer to increasing produc­tivity and health of mature clone GMB 7 tea crop. This research was carried out at the Gambung Experimental Station Block A7 Research Insti­tute for Tea and Cinchona, from July 2013 to September 2013. Randomized block design was used in this study with ten treatments and three replications i.e.: A1H0 = anorganic fertilizer (75%), A2H0 = anorganic fertilizer (50%), A1H1 = anorganic fertilizer (75%) + 2 liter consortium bio fertilizer, A1H2 = anorganic fertilizer (75 %) + 4 liter consor­tium bio-ferti­lizer, A2H1 = anorganic fertilizer (50 %) + 2 liter consortium bio fertilizer, A2H2 = anorga­nic fertilizer (50%) + 4 liter consor­tium bio-fertilizer, A1H3 = anorganic fertilizer (75 %) + 2 liter endophytic bio fertilizer, A1H4 = anor­ganic fertilizer (75%) + 4 liter endo­phytic bio fertilizer, A2H3 = anorganic fertili­zer (50%) + 2 liter endophytic bio fertilizer and A2H4 = anorganic fertilizers (50%) +4 liter endophytic bio fertilizer. The results showed there were a significantly difference on amount of the pekoe shoot among treatments, although the others variable was not significantly diffe­rent. However, the overall consortium bio-fertilizer resulted in the production of shoots 15,36% and endophytic biofertilizer application resulted in the production of shoots 21,93% higher than the control (without bio fertilizer). When compared to the first plucking, the num­ber of shoot from July to September showed increasing trend, so did the number of banji shoots.

In-vitro experiment of nitrogen fixing capacity of indigenous and exogeneous Azotobacter sp at Andisol tea planting area’s

Jurnal Penelitian Teh dan Kina Vol 17, No 1 (2014)
Publisher : Research Institute for Tea and Cinchona

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Abstract

An in-vitro experiment to evaluate total nitrogen content of soil of tea plantation area included with indigenous and exogenous Azoto­bacter sp have been conducted in the Biology and Soil Biotechnology Laboratory, Padja­djaran University. A completely randomized design with 11 treatments and three repli­cations was used. The treatments were: A (ne­gative control); B (positive control); C (A. Kedelai II); D (A. vinelandii); E (A. N.D.9.3); F (A. Padi); G (VI-1); H (II-1); I (V-2); J (I-1); and K (III-2). Size of microbe inoculated popu­lation for each treatment was 1,2 x 1011 CFU/ml. One percent dose was used for ste­rilized soil originated from the tea plantation area. After five days of incubation period re­vealed that II-1 Azotobacter sp produced very significantly different in total nitrogen pro­duction than the other Azotobacter sp, i.e. 0,8532%. Orthogonal comparisons indicated that indigenous Azotobacter sp had the capacity of nitrogen fixation 7,67% higher than that the capacity of nitrogen fixation of exogenous Azotobacter sp for growth on Andisol soil of tea plantation area with pH of 4,5-5,6. Although, Azotobacter sp is very rarely found in soil with low pH (high acidity), this phenomena indi­cating that an active biofertilizer of indigenous Azotobacter sp is present in tea plantation area.

The application of technology portable fertigation system in the dry month in tea plantation

Jurnal Penelitian Teh dan Kina Vol 18, No 1 (2015)
Publisher : Research Institute for Tea and Cinchona

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Abstract

The research aims at providing multi­function both irrigation and fertilizer appli­cations (fertigation) and calculates the produc­tion of shoots and evaluated plant health during dry season. This research was carried out on Gambung Research Station using clone GMB7. Randomized block design was used in this study with six treatments and four replications. The treatments i.e. a. control (without irrigation, without fertilization); b. without irrigation, fer­ti­lization through the soil; c. irrigation 7 mm/day every 3 days (without fertilization); d. irrigation 7 mm/day every 6 days (without fertilization); e. fertigation 7 mm/day every 3 days; and f. fertigation 7 mm/day every 6 days. Observations were made from September-October 2014 with 4 times of plucking. All treatments were significantly different on the yield, number of both pecco and banji. The fertigation treatment using 21 mm of water (7 mm/day every 3 days) showed the highest shoot production of 15,56%. The highest number of pecco shoots and ratio of the number pecco/number of banji in treatment using 21 mm of water (7 mm/day every 3 days) of 32,25%. The highest number of banji found in the control treatment (without irrigation without fertilization) was 74,94%. From this research it can be concluded that the fertigation treatment can give good impact to production maintain and plant health in the dry season.

Comparison of some exogeneous solubillizing phosphate bacteria on Andisol as Indonesia dominant native tea soil

Jurnal Penelitian Teh dan Kina Vol 18, No 2 (2015)
Publisher : Research Institute for Tea and Cinchona

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Abstract

Tea was planting an upland area with Andisol as the dominant soil ordo. Andisol has a special characteristic i.e. low soil pH and it has more mineral that causing low of soil phos-phate soluble, especially for tea plants, there-fore the rule of solubilizing phosphate bacteria will be needed. Laboratory scale experiments were conducted to study the capability activities of some solubilizing phosphate bacterias name-ly Pseudomonas cepaceae, P. malei, Bacillus mycoides, and B. subtilis. The experiment was arranged in Completely Randomized Design with six treatments and four replications. The result on statistical analysis shown that there were no significant differences in increasing of the soil phosphate soluble. Nevertheless, the treatment D (Bacillus mycoides) produced the highest soil phosphate soluble. The capability of bacteria to increase soil phosphate soluble was depended on its habitat, especially soil pH. Even all of the isolates were the superior solubilizing phosphate bacterias in agricultural food plants with neutral soil pH, but they have not significantly different on Andisol. There-fore, the using of indigenous isolates on tea plant areas was suggested.