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POTENSI LACTOBACILLUS YANG DIISOLASI DARI AIR SUSU IBU UNTUK MENCEGAH DIARE [Potential of Lactobacillus Isolated from Breast Milk to Prevent Diarrheae] Nuraida, Lilis; Hana, .; Hartanti, Apriliana W.; Prangdimurti, Endang
Jurnal Teknologi Dan Industri Pangan Vol 23, No 2 (2012): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (480.476 KB) | DOI: 10.6066/6154

Abstract

POTENSI LACTOBACILLUS YANG DIISOLASI DARI AIR SUSU IBU UNTUK MENCEGAH DIARE [Potential of Lactobacillus Isolated from Breast Milk to Prevent Diarrheae] Lilis Nuraida1, 2)*, Hana1, 2), Apriliana W. Hartanti2), dan Endang Prangdimurti1, 2) 1) South East Asian Food and Agriculture Science and Technology (SEAFAST) Center, Institut Pertanian Bogor, Bogor 2) Departemen Ilmu dan Teknologi Pangan, Institut Pertanian Bogor, Bogor   Diterima 09 Desember 2011 / Disetujui 03 Desember 2012 ABSTRACT                                                                                                     Some of Lactobacillus species isolated from breast milk are known to have antimicrobial activities, including against Escherichia coli. The aims of this study were to evaluate the antimicrobial activity of Lactobacillus species isolated from breast milk against enteropathogenic Escherichia coli strain K1.1 and the effectiveness of the Lactobacillus isolates to prevent diarrhea on rats (Sprague Dawley). The infective dose of E. coli K1.1 to induce diarrhea without causing death were also determined. Based on the antimicrobial activity there were three isolates of Lactobacillus that exhibited good inhibition againts E. coli K1.1, i.e. Lactobacillus rhamnosus R14, L. rhamnosus R23, and L. rhamnosus B16. Determination of E. coli infective dose showed that 108 CFU of E. coli K1.1 was sufficient to induce diarrhea on rat without causing death. The number of diarrhea rats and severity level in group treated with L. rhamnosus were lower than groups untreated with the Lactobacilli. This study showed that the three L. rhamnosus isolated from breast milk were able to prevent diarrhea due to infection of E. coli K1.1 when the Lactobacillus was regularly introduced prior to infection. L. rhamnosus R23 showed the best capabilities of preventing diarrhea in rats as compared to two other isolates of Lactobacillus. The incidence of diarrhea correlated with the number of lactobacilli in the feces. However when the period of diarrhea ceased, there were no difference in total lactobacilli and E. coli in the caecum, colon and feces between rats treated with L. rhamnosus and the control. This finding revealed the L. rhamnosus isolated from breast milk were potential for prevention of diarrhea when consumed regularly.   Keywords: antimicrobial activity, breast milk, diarrhea, E. coli, Lactobacillus rhamnosus ABSTRAK   Beberapa spesies Lactobacillus yang diisolasi dari air susu ibu (ASI) diketahui memiliki aktivitas antimikroba, termasuk terhadap Escherichia coli. Tujuan penelitian ini adalah mengevaluasi aktivitas antimikroba spesies Lactobacillus asal ASI terhadap Escherichia coli enteropatogenik strain K1.1 dan efektivitas Lactobacillus rhamnosus untuk mencegah diare pada tikus (Sprague Dawley). Dosis infeksi E. coli K1.1 yang dapat menginduksi diare tanpa menyebabkan kematian juga ditentukan. Berdasarkan aktivitas antimikroba, diperoleh tiga isolat Lactobacillus yang memiliki penghambatan yang baik terhadap E. coli K1.1, yaitu Lactobacillus rhamnosus R14, L. rhamnosus R23, dan L. rhamnosus B16. Penentuan dosis infeksi oleh E. coli menunjukkan bahwa sebanyak 108 CFU E. coli K1.1 merupakan jumlah yang cukup untuk menginduksi diare pada tikus tanpa menyebabkan kematian. Jumlah tikus yang mengalami diare dan keparahannya pada tikus yang diberi L. rhamnosus lebih rendah dibandingkan dengan tikus yang tidak diberi L. rhamnosus namun diinfeksi dengan E. coli K1.1. Hasil penelitian ini menunjukkan bahwa tiga L. rhamnosus asal ASI mampu menghambat diare karena E. coli K1.1 jika Lactobacillus tersebut diberikan secara reguler sebelum terjadinya infeksi. L. rhamnosus R23 merupakan isolat dengan kemampuan yang paling baik dalam mencegah diare dibandingkan dengan dua isolat L. rhamnosus lainnya. Jumlah tikus yang mengalami diare berkorelasi dengan jumlah laktobasili di dalam feses. Akan tetapi jika periode diare telah berhenti, tidak ada perbedaan jumlah total laktobasili dan E. coli dalam sekum, kolon dan feses antara tikus yang diberi L. rhamnosus dan kontrol. Penelitian ini menunjukkan bahwa L. rhamnosus asal ASI berpotensi untuk mencegah diare jika dikonsumsi secara reguler.   Kata kunci: aktivitas antimikroba, air susu ibu, diare, E. coli, Lactobacillus rhamnosus  
Antioxidant Activity of Suji (Pleomele angustifolia N.E. Brown) Leaf Extract Prangdimurti, Endang; Muchtadi, Deddy; Astawan, Made; Zakaria, Fransiska R
Jurnal Teknologi Dan Industri Pangan Vol 17, No 2 (2006): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.844 KB) | DOI: 10.6066/420

Abstract

Numerous studies showed that chlorophyll and its derivatives had antioxidant activity. This research was conducted to obtain the chlorophyll-rich suji leaf liquid extract (SLE) and then to investigate the oral administration this extract on the antioxidant status by means of animal study. The use of Tween 80 0.75% in sodium citrate 12 mM solution as an extraction solution combined with 30 minutes incubation time at 70-750C increased significantly the total chlorophylls content, the water-soluble chlorophylls content and the antioxidant activity of the yielded extract, compared to the suji leaf water-only extract. Two-month oral administration of SLE to male Sprague Dawley rats showed that the liver MDA level significantly decreased, and the liver catalase and superoxide dismutase antioxidant enzyme activities significantly increased compared to the control group. It was suggested that the chlorophyll content in SLE might increase the antioxidant status of animal tested. Key words: suji (Pleomele angustifolia N.E. Brown), chlorophyll, antioxidant
POTENSI ANTIOKSIDAN GEL DAN DAUN KACAPIRING (Gardenia jasminoides Ellis) Widnyana Yoga, IB Ketut; Andarwulan, Nuri; Prangdimurti, Endang
Prosiding Seminar Nasional MIPA Vol 1, No 1 (2011): SEMINAR NASIONAL FMIPA UNDIKSHA 2011
Publisher : Prosiding Seminar Nasional MIPA

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Abstract

Abstract: Leaf of kacapiring (Gardenia jasminoides Ellis) is a part of plant which is having component that can form gel. It has chlorophyll pigment and phenolic compound. The aims of this research were to identificate bioactive compound in leaves and gel which has antioxidant potency. Chlorophyll total was analyzed by spectrophotometer and its derivations of aceton extract (99.9%) by thin layer chromatography (TLC), meanwhile phenolic total and antioxidant capacity were analyzed by spectrophotometer. The result showed that bioactive component of kacapiring leaves and gel were chlorophyll total of 4926.25+190.31 and 1166.86+8.73  mgKg-1db. Both of them had 5 fractions by acetone extract, i.e. chlorophyll a, chlorophyll b, lutein (chlorophyll derivates), feofitine and carotene. Phenolic total in leaves and gel contained 5215.91+2.97 and 2648.16+56.22 GAE/g db, and antioxidant capacity had 1.5 x 10-1+0.00 and 3.1x10-3 +0.00 mM TEAC/mg dw respectively
Potensi rumput laut: Kajian komponen bioaktif dan pemanfaatannya sebagai pangan fungsional Erniati, Erniati; Zakaria, Fransiska Rungkat; Prangdimurti, Endang; Adawiyah, Dede Robiatul
Acta Aquatica: Aquatic Sciences Journal Acta Aquatica: Aquatic Sciences Journal, Vol. 3: No. 1 (April, 2016)
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (752.368 KB) | DOI: 10.29103/aa.v3i1.332

Abstract

Rumput laut merupakan sumber daya hayati yang sangat berlimpah di perairan Indonesia. Namun demikian pemanfaatannya untuk pengolahan produk pangan sangat terbatas, terutama untuk produk pangan fungsional. Rumput laut berpotensi dikembangkan sebagai produk pangan fungsional karena mengandung zat gizi dan komponen bioaktif yang berkhasiat untuk kesehatan. Rumput laut mengandung sejumlah komponen bioaktif seperti senyawa fenolik, pigmen alami, polisakarida sulfat, serat dan komponen bioaktif lainnya yang telah diteliti berkhasiat untuk kesehatan. Untuk dapat dikembangkan sebagai produk pangan fungsional, rumput laut yang digunakan harus bebas dari cemaran logam berat dan bahan pencemar lainnya, harus mengandung komponen bioaktif dan zat gizi yang tinggi sehingga harus ada penerapan standar penanaman dan penanganan pasca panen yang baik di tingkat petani rumput laut. Selain itu Proses pengolahan pangan yang diterapkan tidak merusak komponen bioaktif yang terkandung dalam rumput laut. Optimalisasi pengolahan rumput laut sebagai produk pangan fungsional merupakan alternative pemanfaatan potensi rumput laut Indonesia yang dapat meningkatkan nilai ekonomi rumput laut dan yang lebih penting dapat menyediaakan akses pangan sehat bagi masyarakat luas.Seaweed is a living resource that is abundantly available in Indonesian water. However, its utilization in food processing is very limited, especially as functional food products. Seaweed has the potential to be developed as functional food products because it has nutrient and bioactive components that are beneficial for health. Seaweed has a number of bioactive components such as phenolic compound, natural pigment, polysaccharide sulphate, fiber and other bioactive components that has been studied to be advantageous for health. For a seaweed to be developed into functional food product, it must be free from heavy metal and other pollutant contamination, and must contain bioactive components and high nutrients, thus, a good cultivation and postharvest handling standard have to be applied in seaweed farmer level. Moreover, the food processing applied should not damage the bioactive component within the seaweed. Optimization of seaweed processing into functional food product is an alternative for seaweed potential utilization in Indonesia, which could improve the economic value of the seaweed, and more importantly it could provide access for healthy food for community.
SUPLEMENTASI MIKROENKAPSULAT EKSTRAK KULIT BUAH MANGGIS (KBM) MENURUNKAN KADAR MALONALDEHIDA HATI TIKUS Febriane, Nesya Nova; Giriwono, Puspo Edi; Koswara, Sutrisno; Prangdimurti, Endang
Penelitian Gizi dan Makanan (The Journal of Nutrition and Food Research) Vol 38, No 1 Jun (2015)
Publisher : Pusat Teknologi Terapan Kesehatan dan Epidemiologi Klinik

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Abstract

ABSTRACTHigh consumption of fried food contributes to increased risk of degenerative. Potent antioxidants that may alleviate this problem are contained in pericarp of mangosteen (KBM). However, its bitter taste hinders use of this antioxidant. Microencapsulation process can mask bitter taste and control the release of bioactive compounds. This study aims to evaluate the effectiveness of microencapsulated mangosteen pericarp extract in supressing malonaldehyde (MDA) in rat liver as a result of the consumption of oxidized palm oil. Antioxidants were extracted with methanol from KBM and microencapsulated using gelatin, carboxymethylcellulose (CMC) and maltodextrin. Its antioxidative capacity is determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Extract is supplemented to feed of rats at doses of 100mg/kg bw (KBM 1), meanwhile microencapsulated KBM at doses 100 (KBM 2) and 200 mg/kg bw (KBM 3) in addition to oxidized palm oil, for 50 days. After termination, liver was excised and liver MDA concentration was assayed. The decrease of MDAlevels on KBM 1, KBM 2, and KBM 3 respectively are 11.64 percent, 40.18 percent, and 53.43 percent. Supplementation of microencapsulated and non-encapsulated KBM extract do not affect body weights and feedconsumption of rats. Microencapsulated KBM is effective to reduce MDA levels significantly than its raw extract, in which 200 mg/kg bw is the best concentration. Its process can reduce the bitter taste of KBM.Keywords: antioxidant, mangosteen pericarp, oxidized oil, microencapsulation, liver MDA levelsABSTRAKTingginya konsumsi pangan yang digoreng meningkatkan resiko penyakit degeneratif. Salah satu antioksidan yang berpotensi mengatasi masalah ini adalah yang terkandung pada kulit buah manggis (KBM). Tetapi rasapahit dan getir yang terkandung dalam KBM menyebabkan penggunaannya menjadi terbatas. Oleh karena itu, digunakan proses mikroenkapsulasi yang dapat melindungi dan mengontrol pelepasan senyawa bioaktif yang terkandung dalam KBM. Penelitian ini bertujuan untuk mengevaluasi efektivitas mikroenkapsulasi ekstrak KBMuntuk menurunkan kadar malonaldehida (MDA) hati tikus percobaan yang mengonsumsi minyak sawit teroksidasi. Antioksidan diekstrak dari tepung KBM dengan metanol dan dimikroenkapsulasi menggunakan gelatin, karboksimetil selulosa (CMC), dan maltodekstrin. Kapasitas antioksidan diukur dengan metode 2,2- diphenyl-1-picrylhydrazyl (DPPH). Ekstrak dengan dosis 100 mg/kg bb (KBM 1) serta mikroenkapsulat KBMdengan dosis 100 (KBM 2) dan 200 mg/kg bb (KBM 3) disuplementasi pada pakan tikus dengan penambahan minyak sawit teroksidasi selama 50 hari perlakuan. Setelah diterminasi, hati tikus diambil lalu diukurkonsentrasi malonaldehida (MDA) yang terkandung. Penurunan kadar MDA pada KBM 1, KBM 2, dan KBM 3 yaitu sebesar 11,64 persen, 40,18 persen, dan 53,43 persen. Suplementasi ekstrak KBM yang dimikroenkapsulasi maupun tanpa enkapsulasi tidak mempengaruhi berat dan konsumsi pakan tikus. Mikroenkapsulat KBM efektif untuk menurunkan kadar MDA hati tikus dibandingkan ekstrak tanpa enkapsulasi, dimana konsentrasi terbaik yaitu 200 mg/kg bb. Proses ekstraksi dan mikroenkapsulasi dapat mengurangi rasa pahit dan getir KBM. [Penel Gizi Makan 2015, 38(1): 61-70]Kata kunci: antioksidan, kulit buah manggis, minyak sawit teroksidasi, mikroenkapsulasi, kadar MDA hati
AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN SUJI (Pleomele angustifolia N.E. Brown) [Antioxidant Activity of Suji (Pleomele angustifolia N.E. Brown) Leaf Extract] Prangdimurti, Endang; Muchtadi, Deddy; Astawan, Made; Zakaria, Fransiska R
Jurnal Teknologi dan Industri Pangan Vol 17, No 2 (2006): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.844 KB)

Abstract

Numerous studies showed that chlorophyll and its derivatives had antioxidant activity. This research was conducted to obtain the chlorophyll-rich suji leaf liquid extract (SLE) and then to investigate the oral administration this extract on the antioxidant status by means of animal study. The use of Tween 80 0.75%  in sodium citrate 12 mM solution as an extraction solution combined with 30 minutes incubation time at 70-75oC increased significantly the total chlorophylls content, the water-soluble chlorophylls content and the antioxidant activity of the yielded extract, compared to the suji leaf water-only extract.  Two-month oral administration of SLE  to male Sprague Dawley rats showed that the liver MDA level significantly decreased, and the liver catalase and superoxide dismutase antioxidant enzyme activities significantly increased compared to the control group. It was suggested that the chlorophyll content in SLE might increase the antioxidant status of animal tested.
POTENSI LACTOBACILLUS YANG DIISOLASI DARI AIR SUSU IBU UNTUK MENCEGAH DIARE [Potential of Lactobacillus Isolated from Breast Milk to Prevent Diarrheae] Nuraida, Lilis; Hana, .; Hartanti, Apriliana W.; Prangdimurti, Endang
Jurnal Teknologi dan Industri Pangan Vol 23, No 2 (2012): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (480.476 KB) | DOI: 10.6066/jtip.2012.23.2.158

Abstract

Some of Lactobacillus species isolated from breast milk are known to have antimicrobial activities, including against Escherichia coli. The aims of this study were to evaluate the antimicrobial activity of Lactobacillus species isolated from breast milk against enteropathogenic Escherichia coli strain K1.1 and the effectiveness of the Lactobacillus isolates to prevent diarrhea on rats (Sprague Dawley). The infective dose of E. coli K1.1 to induce diarrhea without causing death were also determined. Based on the antimicrobial activity there were three isolates of Lactobacillus that exhibited good inhibition againts E. coli K1.1, i.e. Lactobacillus rhamnosus R14, L. rhamnosus R23, and L. rhamnosus B16. Determination of E. coli infective dose showed that 108 CFU of E. coli K1.1 was sufficient to induce diarrhea on rat without causing death. The number of diarrhea rats and severity level in group treated with L. rhamnosus were lower than groups untreated with the Lactobacilli. This study showed that the three L. rhamnosus isolated from breast milk were able to prevent diarrhea due to infection of E. coli K1.1 when the Lactobacillus was regularly introduced prior to infection. L. rhamnosus R23 showed the best capabilities of preventing diarrhea in rats as compared to two other isolates of Lactobacillus. The incidence of diarrhea correlated with the number of lactobacilli in the feces. However when the period of diarrhea ceased, there were no difference in total lactobacilli and E. coli in the caecum, colon and feces between rats treated with L. rhamnosus and the control. This finding revealed the L. rhamnosus isolated from breast milk were potential for prevention of diarrhea when consumed regularly.
Crude Palm Oil Consumption As A Hepatoprotectors To Housewives In Bogor Perdani, Claudia Gadizza; Zakaria, Fransiska R; Prangdimurti, Endang
Jurnal Teknologi Pertanian Vol 17, No 2 (2016)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

Kekurangan Vitamin A (KVA) di Indonesia masih merupakan masalah utama. Kekurangan vitamin A tingkat berat (rabun senja) telah jarang ditemui, tetapi tingkat KVA subklinis masih bisa ditemukan. Minyak sawit mentah (CPO) memiliki kandungan tinggi karotenoid sebagai sumber provitamin A. CPO secara alami berwarna merah karena mengandung karotenoid yang tinggi, dan belum dimanfaatkan di Indonesia. CPO mengandung karotenoid dan vitamin E yang berfungsi sebagai antioksidan bagi tubuh. Antioksidan dapat mengurangi kerusakan sel dengan menetralisir radikal bebas serta mencegah kerusakan lipid, protein, enzim, dan DNA. Tujuh puluh responden dipilih untuk konsumsi CPO selama 2 bulan dengan dosis ± 3.27 ml/hari. Plasma darah dari 22 responden yang sehat dianalisis dengan metode asam Tri Fluoro asetat (TFA), enzim aspartat Transaminase (AST), Alanine transaminase (ALT), Alkaline Phospatase (ALP) kit. Hasil penelitian menunjukkan 16 responden mengalami peningkatan jumlah retinol plasma dan penurunan tingkat aktivitas AST, ALT, dan enzim ALP. Penelitian ini menunjukkan bahwa CPO memiliki aktivitas antioksidan yang dapat meningkatkan kesehatan hati, sehingga dapat menjadi alternatif sumber provitamin A untuk menangani kekurangan vitamin A di Indonesia
PENGARUH MINUMAN DIPERKAYA CINNULIN TERHADAP MDA DAN SENYAWA INFLAMASI PLASMA PENDERITA DMT2 Putri S., Nawasari Indah; Prangdimurti, Endang; Zakaria, Fransiska R.
Jurnal Teknologi dan Industri Pangan Vol 27, No 1 (2016): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (391.667 KB) | DOI: 10.6066/jtip.2016.27.1.51

Abstract

 Cinnamon has been used as a traditional treatment for thousands of years. Aqueous cinnamon extract namely cinnulin is known to have  positive effects on type-2 diabetes mellitus (T2DM). We aimed to study the effect of cinnulin enriched drink rich in polyphenols on plasma malondialdehyde (MDA), interleukin-6 (IL-6) and cyclooxygenase-2 (COX-2) in T2DM patients. 255 mg of cinnulin was mixed with tea extract into a 2.5 gram of instant drink with alumunium foil as the packaging material. A single blind study were performed on 33 respondents with T2DM from Dramaga District. Respondents were divided into three groups, i.e. group given cinnulin enriched drink (1), instant tea drink (2), and none of the products (control) (3). Group 1 and 2 were assigned to drink two sachetsof the products a day for 5 weeks. The MDA content of all groups remain unchanged after treatment although slightly increased (Wilcoxon, P>0.050) and no differences between groups was observed (P>0.050) suggesting that high phenolic content in the producst cause a reverse effect. Both inflammation marker (IL-6 and COX-2) decreased significantly for groups given cinnulin enriched drink and instant tea, whereas there is a significant increase for IL-6 and a significant decrease for COX-2 in control group. These results conclude that cinnulin enriched drink can be used as an antiinflammation drink for T2DM.
PERBAIKAN STATUS ANTIOKSIDAN PENDERITA DIABETES TIPE 2 DENGAN TAHU KEDELAI HITAM KAYA SERAT Triandita, Nanda; Zakaria, Fransiska R.; Prangdimurti, Endang; Putri, Nela Eska
Jurnal Teknologi dan Industri Pangan Vol 27, No 2 (2016): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (384.329 KB) | DOI: 10.6066/jtip.2016.27.2.123

Abstract

Type 2 diabetes mellitus (T2DM) is a non-insulin dependent diabetes, because insulin is not secreted by beta cell pancreas or dysfunction of insulin reseptors. The purposes of this research were to evaluate the effect of dietary fiber rich- tofu from black soybean on the antioxidant plasma status, malonaldehide (MDA) and hepatic marker in T2DM patients. One group (n=11) of patients was given 80 g of tofu for 30 days while the control group (n=11) was not. No significant differences was observed pertaining the characteristics of both groups before interventions (age, body mass index, body weight, body high, systolic blood pressure, diastolic blood pressure and fasting blood glucose). The antioxidant activity, MDA and AST/ALT of both groups were analyzed from plasma at day-0 and day-30. Antioxidant activity was analyzed by DPPH method, MDA level with TBARS method, and AST/ALT by International Federation of Clinical Chemistry (IFCC) method. The result showed that the plasma antioxidant increased (4.77±9.49%), but the MDA (2.11±1.73 nmol/mL) and AST (7.73±16.72 U/L)/ ALT (3.55±12.79 U/L) level decreased. Rich dietary fiber tofu from black soybean may have potential use for improving the health status of T2DM patients.