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Portrait of Hatchery Management Profile on Rainbow Fish Melanotaenia boesemani (Allen & Cross, 1980) Cultivation in Jakarta Area Nugraha, Media Fitri Isma; Avarre, Jean-Christophe; Pouyaud, Laurent; Kadarusman, Kadarusman; Carman, Odang; Junior, M. Zairin
TORANI: Journal of Fisheries and Marine Science Vol 1, No 2 (2018)
Publisher : Marine Science and Fisheries Faculty, Hasanuddin University

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Abstract

Hatchery managers and maintaining genetic diversity and fitness population in endangered and threatened species in pond is a important and difficult thing to do.  But is must to do for conservation biology.  Fitness and structure population depends on effective breeding number (Ne) and population connectivity between each other. The second most important thing is gene flow and genetic drift. Ne is major role in the maintenance of genetic diversity as indicator for inbreeding depresion and genetic drift.  We sampled 6 hatchery in Jakarta  and Bekasi Indonesia region and used 12 polymorphic microsatellite loci to quantify population genetic structure. Result in this study are, each farm have different methode. The old farmers is bapak Gusi and bapak Hasan (+30 years).  Ne in bapak Hasan and bapak Gusi hatchery is 66,667 and F value is 0,00749 and loosing allele (P) is 0,26183.  Compared with younger farmers bapak Yahya (5 years) have Ne = 133,333, F = 0,00375 and P = 0,06855.  Even though hatchery bapak Hasan and bapak Gusi have the same value Ne, F and P but the result in population structure they are different founder populations. Hatchery bapak Hasan have unique structure and alleles composition compared with other hatchery.Keywords: breeding, management, Melanotaenia boesemani, hatchery, population.
STRUKTUR POPULASI DAN SEJARAH KOLONISASI IKAN BOTIA (Chromobotia macracanthus BLEEKER) ASAL SUMATERA DAN KALIMANTAN BERDASARKAN SEKUEN INTRON DARI GEN ALDOLASE-B Sudarto, Sudarto; Pouyaud, Laurent; Kusuma, Ruby V.
Jurnal Perikanan Universitas Gadjah Mada Vol 10, No 2 (2008)
Publisher : Universitas Gadjah Mada

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Abstract

Clown loach (Chromobotia macracanthus Bleeker) or botia is an endemic species of Indonesia belonging to Cobitidae family which has restricted distribution only in Sumatra in Pangabuang, Kwanten, Batanghari, Teluk Betung, Musi riverines, and in Kalimantan found in Barito, Kahayan, Kapuas, Bongan dan Mahakam rivers. The objectives of this study are to find the genetical population structure and the history of colonization of clown loach from Kalimantan (Kapuas river) dan Sumatra (Batanghari, Musi and Tulang Bawang rivers) based on intron sequence of Aldolase B gene. Cutting fins are collected from each location and preserved in ethanol solution and stored in deep freezer before digested for DNA analysis by proteinase-K method followed by CTAB (cethyl/hexadecyl trimethyl ammonium bromide) method; then amplified by EPIC-PCR (Exon Primed Intron Crossing Polymerase Chain Reaction) techniques. PCR product were Electrophoreted and DNA bands were sequenced. The results of sequences analysis showed 8 types of alleles, 5 types for type A alleles (A-1, A-2, A-3, A-4, A-5), 2 type B alleles (B-1 and B-2) and C-1. There was a genetic difference between Sumatra and Kalimantan populations.
SYSTEMATIC REVISION OF THE GENERA OF PANGASIIDAE (SILURIFORMES, OSTARIOPHYSI) Gustiano, Rudhy; Pouyaud, Laurent
Indonesian Aquaculture Journal Vol 3, No 1 (2008): (June 2008)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

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Abstract

The family Pangasiidae belongs to the suborder Siluroidei, order Siluriformes, and suborder Ostariophysi. Since the groups were established as Pangasiini Bleeker, 1858; its content and classification have been greatly changed. Judging from the literature, the main constraint to cultivate wild species and to optimize the production of cultured species is needed to the poorly documented systematics of this family. Therefore the objective of the present study is to provide the diagnostic characters and the keys for identification the genera of Pangasiidae. The results clearly demonstrate that biometrically four natural groups can be distinguished. They are the genus Helicophagus, Pangasianodon, Pteropangasius, and Pangasius. The diagnosis of the family, the identification key of the genera and the description are given.
TAXONOMY AND GENETIC RELATIONSHIPS OF PANGASIIDAE, ASIAN CATFISHES, BASED ON MORPHOLOGICAL AND MOLECULAR ANALYSES Gustiano, Rudhy; Pouyaud, Laurent
Indonesian Aquaculture Journal Vol 2, No 2 (2007): (December 2007)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

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Abstract

Pangasiids are economically important riverine catfishes generally residing in freshwater from the Indian subcontinent to the Indonesian Archipelago. The systematics of this family are still poorly known. Consequently, lack of such basic information impedes the understanding of the biology of the Pangasiids and the study of their aquaculture potential as well as improvement of seed production and growth performance. The objectives of the present study are to clarify phylogeny of this family based on a biometric analysis and molecular evidence using 12S ribosomal mtDNA on the total of 1070 specimens. The study revealed that 28 species are recognised as valid in Pangasiidae. Four genera are also recognized as Helicophagus Bleeker 1858, Pangasianodon Chevey 1930, Pteropangasius Fowler 1937, and Pangasius Valenciennes 1840 instead of two as reported by previous workers. The phylogenetic analysis demonstrated the recognised genera, and genetic relationships among taxa. Overall, trees from the different analyses show similar topologies and confirm the hypothesis derived from geological history, palaeontology, and similar models in other taxa of fishes from the same area. The oldest genus may already have existed when the Asian mainland was still connected to the islands in the southern part about 20 million years ago.
DISTRIBUTION OF TROPICAL EEL GENUS Anguilla IN INDONESIA WATER BASED ON SEMI-MULTIPLEX PCR Fahmi, Melta Rini; Pouyaud, Laurent; Berrebi, Patrick
Indonesian Aquaculture Journal Vol 7, No 2 (2012): (December 2012)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

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Abstract

Tropical eels living in Indonesian waters are known to be composed of several species, but their real listing together with their distribution ranges need to be established. The main difficulties are the very high number of islands with perennial rivers where these species are living during the growth phase of their life cycle. It is difficult, sometimes impossible, to determine the species using morphological characters, moreover on glass eels. In order to establish the geographic distribution of tropical eels of the genus Anguilla in Indonesian waters, a total 1,115 specimens were collected between 2008 and 2012. Sample collection was done in the growth habitats that are rivers and estuaries by commercial nets of different categories according to the fish size. All samples were identified genetically using the recently developed semimultiplex PCR method. We recognized four species and subspecies with wide distribution: Anguilla bicolor bicolor, Anguilla bicolor pacifica, Anguilla marmorata, and Anguilla interioris; two species with limited distribution, close to endemism: Anguilla celebesensis and Anguilla borneensis and one subspecies Anguilla nebulosa nebulosa that is only spread in river flowing into Indian Ocean.
EFFECT AND COMPARISON OF RECYCLING AND STAGNANT FRESHWATER ON PERFORMANCES (GROWTH AND SURVIVAL RATES; FISH QUALITY) AND PROFITABILITY OF THE ORNAMENTAL FISH Barbus schwanefeldi (KAPIAT) REARED AT 4 DIFFERENT DENSITIES Sudarto, Sudarto; Priadi, Agus; Slembrouck, Jack; Pouyaud, Laurent; Subamia, I Wayan
Indonesian Aquaculture Journal Vol 2, No 2 (2007): (December 2007)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

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Abstract

Comparing two different rearing systems in fish production through stagnant and recirculation water systems showed that recirculation system has several benefits such as reducing manpower, and minimize or eliminate in using antibiotics and also eliminate the grow out of diseases, increasing the profits due to increase in density of fish cultured in the system, and water quality remain stable in optimal condition.
RIVERINE CATFISHES OF KALIMANTAN, PANGASIIDAE: DIAGNOSIS, DISTRIBUTTON, AND ECOLOGY Gustiano, Rudhy; Pouyaud, Laurent
Indonesian Fisheries Research Journal Vol 11, No 2 (2005): (December 2005)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

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Abstract

The main constrain to cultivate wild species and optimize the production of cultured species is to due poorly documented of genetic resources. ln the current study, it presents the diversity of pangasiids catfishes from Kalimantan.
PERBEDAAN MORFOLOGIS POPULASI BOH A Botia macracantha ASAL SUMATERA DAN KALIMANTAN [Morphological differences among botia Botia macracantha populations originated from Sumatra and Kalimantan] Sudarto, nFN; Pouyaud, Laurent
Jurnal Iktiologi Indonesia Vol 6, No 2 (2006): Desember 2006
Publisher : Masyarakat Iktiologi Indonesia (MII )

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Abstract

There are two distinct morphological characters of Botia macracantha (Clown loach) originated from Sumatera and Kalimantan. It is assumed that those two populations caused difficulties in breeding program when the populations mixed up. To know the differences of those two populations at least 100 sampels were collected from Sumatra or 50 sampels of each location. Another location was West Kalimantan. Morfometric characters are measured among those populations and statistical analysis is implemented to know the differences among populations observed. The result showed that populations from Sumatra is differ morphometrically from Kalimantan.
IDENTIFICATION KEY BASED ON MORPHOLOGICAL CHARACTERS OF THE SOUTHEAST ASIAN SPECIES OF THE GENUS CLARIAS (PISCES : CLARHDAE) [Kunci Identifikasi Ikan Clarias (Pisces : Clariidae) Asia Tenggara Berdasarkan Ciri Morfologi] Sudarto, nFN; Pouyaud, Laurent
Jurnal Iktiologi Indonesia Vol 5, No 2 (2005): Desember 2005
Publisher : Masyarakat Iktiologi Indonesia (MII )

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Abstract

Enam ratus empat puluh lima spesimen ikan Clarias terutama dikumpulkan dari Vietnam, Thailand dan Indonesia dipelajari ciri morfometriknya dengan cara pengukuran dan penghitungan mens:ik Terdapat 29 titik-titik pengukuran untuk setiap spesimen, 5 penghitungan meristik dan 5 pengamatan morfologi spesifik, Karakter-karakter tersebut (selain panjang standar) dipilih untuk diagnosanya. Data morfometrik diukur menggunakan kalifer dengan ketel itian sampai 0,5 mm. Data tersebut d i anal is is untuk membedakan antara spesies dan untuk menyusun kunci identifikasi.