Bambang Pontjo
Faculty of Veterinary Medicine, Bogor Agricultural University

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Antibacterial activity of temu kunci tuber (kaempheria pandurata) essential oil against Bacillus cereus Miksusanti, Miksusanti; J, Betty S.L.; Syarief, Rizal; Pontjo, Bambang; Mulyadi, Gatot T.
Medical Journal of Indonesia Vol 18, No 1 (2009): January-March
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

Aim The mechanism of temu kunci tuber essential oil potential as antimicrobial agent has not been reported. To analyze the mechanism of antibacterial activity of temu kunci tuber essential oil from Yogyakarta on B.cereus. Antibacterial activity of essential oil were analyzed for its ability to disrupt bacterial cell membrane, that caused cell leakage and altered the morphology of the bacteria. Leakage was measured by analyzing the Ca+2, K+ ion outflow using an atomic adsorption spectrometry (AAS), and protein and nucleic acid using an ultraviolet spectrophotometer (UVS) on 280 nm and 260 nm respectively. Alterations in morphology were assessed using scanning electron microscopy (SEM).Results Minimum inhibitory concentration (MIC) of temu kunci essential oil on B.cereus was 0.12% (v/v). Treatment of B. cereus using 1MIC and 2MIC showed significant leakage compared to control (P<0.05). The K+ and Ca+2 ion leakage from the bacterial cells were between 10.32-35.57% and 15.05-41.54% respectively and showed significant difference compared to control (P<0.05). The absorbance observed by UVS for protein and nucleic acid leakage were 0.6330-0.8670 at 280 nm and 0.4320-0.8307 at 260 nm, respectively, and were significantly different compared to control (P<0.05). Exposure of 1 MIC temu kunci essential oil on B.cereus caused thickening as well as irregularities on the cell wall. At 2 MIC cells seemed transparent, empty looking and showed a ghost-like appearance.Conclusion Kaempheria pondurata essential oil could cause leakage and alter the morphology of the bacteria. (Med J Indones 2009; 18:10-7)Keywords: leakage, morphology
AKTIVITAS SEDIAAN SALEP EKSTRAK BATANG POHON PISANG AMBON (Musa paradisiaca var sapientum) DALAM PROSES PERSEMBUHAN LUKA PADA MENCIT (Mus musculus albinus) Febram, Bayu; Wientarsih, Ietje; Pontjo, Bambang
Majalah Obat Tradisional Vol 15, No 3 (2010)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

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Abstract

Tujuan dari penelitian ini adalah untuk mengetahui aktivitas pemberian ekstrak dari batang pohon pisang Ambon (Musa paradisiaca var sapientum) dalam bentuk sediaan salep terhadap proses persembuhan luka pada kulit mencit (mus musculus albinus) melalui pengamatan patologi anatomi dan histopatologi.  Hewan yang digunakan dalam penelitian ini adalah mencit dengan strain DDY umur 4-6 minggu sebanyak 45 ekor yang dibagi menjadi tiga kelompok, yaitu kelompok kontrol negatif (salep placebo), kontrol positif (salep komersil) dan salep ekstrak batang pohon pisang Ambon.  Semua mencit dilukai pada daerah punggung anterior sepanjang 1-1,5 cm menggunakan skalpel. Setiap hari luka diolesi dua kali dengan salep yang diuji ( salep placebo, salep komersil, dan salep ekstrak). Pengamatan patologi anatomi dilakukan setiap hari sementara pengamatan  histopatologi dilakukan pada hari ke 3, 5, 7, 14 dan 21 pasca perlukaan mencit yang dieuthanasia. Parameter pengamatan patologi anatomi adalah warna luka, pembekuan darah, terbentuknya keropeng dan ukuran luka. Parameter yang diamati pada sediaan  histopatologi adalah infiltrasi   sel-sel radang (neutrofil, limfosit, makrofag), neokapilerisasi, persentase re-epitelisasi dan ketebalan fibroblas.  Semua data kuantitatif diuji secara statistik menggunakan Analisa Sidik Ragam (ANOVA) dan dilanjutkan dengan uji wilayah berganda Duncan, sedangkan data kualitatif disajikan secara deskriptif.  Hasil penelitian menunjukkan bahwa pada pengamatan patologi anatomi kelompok salep ekstrak lebih cepat membentuk keropeng dan menutup luka tanpa bekas, jika dibandingkan dengan kontrol negatif. Hasil uji statistik pada parameter infiltrasi sel-sel radang pada kelompok salep ekstrak menunjukan hasil yang berbeda nyata (P<0.05) dengan kelompok kontrol negatif. Hasil pengamatan histopatologis menunjukkan bahwa ekstrak batang pohon pisang Ambon dalam sediaan salep mampu meningkatkan jumlah infiltrasi dari sel-sel radang, meningkatkan pembentukan neokapiler, meningkatkan persentase re-epitelisasi serta  mempercepat pembentukan fibroblas dibandingkan dengan kelompok kontrol negatif. Berdasarkan hasil diatas dapat disimpulkan bahwa pemberian salep ekstrak  batang pohon pisang ambon mempercepat proses persembuhan luka.
The influence of using platelet rich plasma for post-extraction wound healing process Oscar, Franky; Mangundjaja, Sunardhi; Yusuf, Makmuri; Pontjo, Bambang
Padjadjaran Journal of Dentistry Vol 22, No 1 (2010): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

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Abstract

Wound healing process characterized by specific processes of inflammation, proliferation, and maturation. It was coordinated by the regenerating and healing factors, including growth factor. Platelet-rich plasma was a blood component that separated from blood cell plasma, which contains a lot of growth factors for increased wound healing. The objective of this research was to observe the effect of the application of platelet-rich plasma for wound healing. The true experimental factorial design research was done to 24 rabbits (Oryctolagus cuniculus) which have been performed tooth extraction and application of platelet-rich plasma. These rabbits were terminated on 3rd, 7th, and 14th day, and the lower jaw region where the tooth had been extracted were cropped stained with hematoxylin-eosin for fibroblast and collagen histopathology examination. Based on ANOVA statistical analysis, the mean of fibroblast on the experimental group was 52.00+19.18 higher than the control group 24.83+10.46. Statistical tests showed a significant result of platelet-rich plasma application to fibroblast amount (p < 0.001). Collagen mean on the experimental group was 30.25+9.33 higher than the control group 13.75+6.96. It was significant statistically (p = 0.001). The conclusion of this research that wound healing process was influenced by the application of platelet-rich plasma.
Comparison of the effect of collagen (amino acid) and amnion (proteinase inhibitor) on the wound healing of soft tissues Adhiarto, Weko; Mangundjaja, Sunardhi; Yusuf, Makmuri; Pontjo, Bambang
Padjadjaran Journal of Dentistry Vol 22, No 1 (2010): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

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Abstract

The wound healing process of the soft tissue aims to accelerate the closing of the wound by recurring, restoring the function, and minimizing the scar tissue. This process has to occur in the surgery process to obtain better-wound healing. The aim of this study was to know the stimulation effect of the wound dressing to accelerate the wound healing process of the soft tissue using Collagen (amino acid) and Amnion (proteinase inhibitors). This true experimental study was done to 36 mice (Sprague Dawley) that were divided into three different groups, two groups were the treatment groups and one group was the control group. Each group was adapted in Pathology Laboratory of Faculty of Veterinary Medicine Bogor Agricultural University. All of the mice were injured on the skin by incising the right side of the back for 2 cm in length and 4 mm in depth and then cleaned with 0.9% NaCl solution. To the first group of treatment, the wound was applicated by Amnion, and the second group was applicated by Collagen. Each 4 mice of all group were a termination by ether and encryption on the wounded tissue was conducted on day 3rd, 7th, and 14th. Assessment for a number of fibroblast and collagen synthesis on the wound of back tissue was conducted with the histological examination by painted using hematoxylin-eosin. The results concluded that Collagen application was influencing the mean of fibroblast higher than Amnion, and the lowest was controlled with statistically significant. The mean of collagen used Collagen lower than Amnion, and the lowest was control with statistically significant. It could be concluded that collagen application was preeminent in increasing the productivity of the fibroblast, as well as in developing forms of the collagen synthesis compared with Amnion and control.
The influence of using platelet rich plasma for post-extraction wound healing process Oscar, Franky; Mangundjaja, Sunardhi; Yusuf, Makmuri; Pontjo, Bambang
Padjadjaran Journal of Dentistry Vol 22, No 1 (2010): March
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Original Source | Check in Google Scholar | Full PDF (683.555 KB)

Abstract

Wound healing process characterized by specific processes of inflammation, proliferation, and maturation. It was coordinated by the regenerating and healing factors, including growth factor. Platelet-rich plasma was a blood component that separated from blood cell plasma, which contains a lot of growth factors for increased wound healing. The objective of this research was to observe the effect of the application of platelet-rich plasma for wound healing. The true experimental factorial design research was done to 24 rabbits (Oryctolagus cuniculus) which have been performed tooth extraction and application of platelet-rich plasma. These rabbits were terminated on 3rd, 7th, and 14th day, and the lower jaw region where the tooth had been extracted were cropped stained with hematoxylin-eosin for fibroblast and collagen histopathology examination. Based on ANOVA statistical analysis, the mean of fibroblast on the experimental group was 52.00+19.18 higher than the control group 24.83+10.46. Statistical tests showed a significant result of platelet-rich plasma application to fibroblast amount (p < 0.001). Collagen mean on the experimental group was 30.25+9.33 higher than the control group 13.75+6.96. It was significant statistically (p = 0.001). The conclusion of this research that wound healing process was influenced by the application of platelet-rich plasma.
Comparison of the effect of collagen (amino acid) and amnion (proteinase inhibitor) on the wound healing of soft tissues Adhiarto, Weko; Mangundjaja, Sunardhi; Yusuf, Makmuri; Pontjo, Bambang
Padjadjaran Journal of Dentistry Vol 22, No 1 (2010): March
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Original Source | Check in Google Scholar | Full PDF (2000.307 KB)

Abstract

The wound healing process of the soft tissue aims to accelerate the closing of the wound by recurring, restoring the function, and minimizing the scar tissue. This process has to occur in the surgery process to obtain better-wound healing. The aim of this study was to know the stimulation effect of the wound dressing to accelerate the wound healing process of the soft tissue using Collagen (amino acid) and Amnion (proteinase inhibitors). This true experimental study was done to 36 mice (Sprague Dawley) that were divided into three different groups, two groups were the treatment groups and one group was the control group. Each group was adapted in Pathology Laboratory of Faculty of Veterinary Medicine Bogor Agricultural University. All of the mice were injured on the skin by incising the right side of the back for 2 cm in length and 4 mm in depth and then cleaned with 0.9% NaCl solution. To the first group of treatment, the wound was applicated by Amnion, and the second group was applicated by Collagen. Each 4 mice of all group were a termination by ether and encryption on the wounded tissue was conducted on day 3rd, 7th, and 14th. Assessment for a number of fibroblast and collagen synthesis on the wound of back tissue was conducted with the histological examination by painted using hematoxylin-eosin. The results concluded that Collagen application was influencing the mean of fibroblast higher than Amnion, and the lowest was controlled with statistically significant. The mean of collagen used Collagen lower than Amnion, and the lowest was control with statistically significant. It could be concluded that collagen application was preeminent in increasing the productivity of the fibroblast, as well as in developing forms of the collagen synthesis compared with Amnion and control.