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Journal : International Journal of Applied Biology

The Analysis of β-cryptoxanthin and Zeaxanthin using HPLC in the Accumulation of Orange Color on Lowland Citrus Sumiasih, Inanpi Hidayati; Poerwanto, Roedhy; Efendi, Darda; Agusta, Andria; Yuliani, Sri
International Journal of Applied Biology Vol 1, No 2 (2017): International Journal of Applied Biology
Publisher : Hasanuddin University

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Abstract

Citrus peel color is one of the main quality attributes which was caused by the accumulation of carotenoids and its derivatives, especially β-citraurine. It makes citrus peel color looks attractive (orange). The orange color is a mixture of β-cryptoxanthin with β-citraurin. The objectives of this study were (1) to observe the effect of precooling and duration of proper ethylene exposure in the formation of orange color on citrus peel, (2) to identify and determine the β-cryptoxanthin content and total chlorophyll on citrus peel. Citrus was from Tuban, East Java while the study was conducted at PKHT IPB and LIPI. Precooling and without precooling treatment prior to injection of 100 ppm of ethylene exposed at 15 °C, duration of exposure control (0), 24, and 48 hours. The results show that the best color of the Citrus Color Index (CCI) is the precooling treatment and the duration of ethylene exposure for 24 hours, which can reduce total chlorophyll content about 8 times and proved to increase β-cryptoxanthin pigment content five times in accelerating the formation of orange citrus reticulata peel color to bright orange. Degreening has no significant effect on total dissolved solids and the firmness level of citrus fruits.Keyword: β-cryptoxhantin; citrus; chlorophyll; degreening; ethylene zeaxanthin.
The Analysis of β-cryptoxanthin and Zeaxanthin using HPLC in the Accumulation of Orange Color on Lowland Citrus Sumiasih, Inanpi Hidayati; Poerwanto, Roedhy; Efendi, Darda; Agusta, Andria; Yuliani, Sri
International Journal of Applied Biology Vol 1, No 2 (2017): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (1035.254 KB)

Abstract

Citrus peel color is one of the main quality attributes which was caused by the accumulation of carotenoids and its derivatives, especially β-citraurine. It makes citrus peel color looks attractive (orange). The orange color is a mixture of β-cryptoxanthin with β-citraurin. The objectives of this study were (1) to observe the effect of precooling and duration of proper ethylene exposure in the formation of orange color on citrus peel, (2) to identify and determine the β-cryptoxanthin content and total chlorophyll on citrus peel. Citrus was from Tuban, East Java while the study was conducted at PKHT IPB and LIPI. Precooling and without precooling treatment prior to injection of 100 ppm of ethylene exposed at 15 °C, duration of exposure control (0), 24, and 48 hours. The results show that the best color of the Citrus Color Index (CCI) is the precooling treatment and the duration of ethylene exposure for 24 hours, which can reduce total chlorophyll content about 8 times and proved to increase β-cryptoxanthin pigment content five times in accelerating the formation of orange citrus reticulata peel color to bright orange. Degreening has no significant effect on total dissolved solids and the firmness level of citrus fruits.Keyword: β-cryptoxhantin; citrus; chlorophyll; degreening; ethylene zeaxanthin.
Co-Authors ', Sobir , Dorly , Kasutjianingati , Lizawati , Sakhidin , Sobir , Sudarsono , Trikoesoemaningtyas , Widodo , Yudiwanti - Juanasri . SUHARSONO ABDUL MUNIF Abdul Qadir Achmad Surkati AGUS PURWITO Ahmad Ghozi Manshuri Ahmad Ghozi Mansyuri Ahmad S. Abidin Akmal, Ajmir Ali Nurmansyah Anas D Susila Anas D. Susila Anas Dinurrohman Susila ANDRIA AGUSTA Ani Kurniawati Ansyori Ansyori, Ansyori Asmini Budiani Bambang S . Purwoko Bambang S. Purwoko Bambang Sapta Purwoko Cenra Intan Hartuti Tuharea CICIK SURIANI D Fatria Darda Efendi Darda Effendi Darmawan Darmawan DEDY DURYADI DEWI SUKMA DIDY SOPANDIE Djoko Santoso DORLY DORLY E Efendi, E Edi Santosa Eko Setiawan Endang Gunawan Fauziyah Harahap Fumio Fukuda H., Nian Rimayanti HAJRIAL ASWIDINNOOR Hanifah Muthmainnah, Hanifah Heni Purnamawati Herry Cahyana Hiroshi Inoue I Hidayati, I I MADE ARTIKA I NYOMAN RAI Iman Rusmana Inanpi Hidayati Sumiasih, Inanpi Hidayati Indah Wulandari Iskandar Lubis Jawal Muhammad Anwarudinsyah JULIARNI JULIARNI Jumawati, Riana Kasutjianingati . Ketty Suketi Kuniyuki Saitoh Kurniadinata, Odit F. Kurniawan, Vandra La Ode Safuan Latifah K. Darusman LATIFAH KOSIM DARUSMAN Lukman Liferdi Maulana, Mohamad Akhbar Maulana, Mohamad Akhbar Memen Surahman Muhammad Arif Nasution Muhammad Darmawan, Muhammad Nadhirah Karimatul Ilmi, Nadhirah Karimatul Naohiro Kubota Nobuo Sugiyama Nono Sutrisno Nur Wahyu Sariningtias, Nur Wahyu Nurfitri Ramadhani, Nurfitri Nurul Khumaida Odit Ferry Kurniadinata, Odit Ferry Rahmat Budiarto Ramdan Hidayat Rd. Selvy Handayani Resa Sri Rahayu, Resa S Susanto SAMANHUDI, . Septirosya, Tiara Slamet Susanto Soaloon Sinaga sobir . SOEKISMAN TJITROSEMITO Sri Astuti Rais Sri Yuliani SRIANI SUJIPRIHATI Suci Rahayu SUDARSONO SUDARSONO SUDIRMAN YAHYA SURYO WIYONO Sutrisno sutrisno T Purnama Tanari, Yulinda Taruna Shafa Arzam, Taruna Shafa Tetty Chaidamsari, Tetty Titin Purnama TRI KOESOEMANINGTYAS Tri Muji Ermayanti Widodo . Widya Sari winarso D widodo Winarso D. Widodo Winarso D.Widodo, Winarso Winarso Dradjad Widodo Winarso Drajad Widodo Y A Purwanto, Y A Yunita, Roza Yunus, Ismadi