D Piedrafita
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In vitro studies: The role of immunological cells in Indonesian thin tail sheep in the killing of the liver fluke, Fasciola Estuningsih, S.E; Wiidjajanti, S; Partoutomo, S; ., Spithill; Raadsma, H; Piedrafita, D
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 2 (2002): JUNE 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.304 KB) | DOI: 10.14334/jitv.v7i2.284

Abstract

Previous studies have shown that Indonesian Thin Tail (ET) sheep exhibit high resistance to challenge with Fasciola gigantica when compared with Merino sheep, and this resistance is expressed in early infection. In order to study the role of the immune system in this resistance to ET sheep, in vitro studies were undertaken in the laboratory. In vitro study to confirm the ability of immune cells from ET sheep in the killing of F. gigantica larvae has been done by incubating immune cells and F. gigantica larvae together with immune sera or normal sera. The viability of the larvae was observed over a period 3 days incubation by observing their motility. The results showed that the cells isolated from F. gigantica- challenged ET sheep in the presence of immune sera from ET were able to kill 70% of the larvae. In contrast, cells from infected Merino were unable to kill a significant number of F. gigantica using the same sera source. It seems that the cytotoxicity was dependent on the presence of immune sera and ET peritoneal cells, suggesting the potential role of an antibody-dependent cell cytotoxic (ADCC) mechanism in the resistant ET sheep. Key words: In vitro, Fasciola gigantica, peritoneal cell, sheep gigantica.
The responses of eosinophil and packed cell volume (PCV) on sheep infected with Fasciola gigantica Widjajanti, S; Estuningsih, S Endah; Partoutomo, Subandriyo; Raadsma, H.W; Spithill, T.W; Piedrafita, D
Indonesian Journal of Animal and Veterinary Sciences Vol 7, No 3 (2002)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v7i3.295

Abstract

The responses of eosinophil and packed cell volume (PCV) values were verified in infected sheep, in order to identify whether these parameters could be used to predict the flukes burden and their correlation with breed resistance. Fifteen Indonesian thin tail sheep (ET), 9 Merino sheep and 148 backcross sheep generated from mating of Merino sheep and F1 sheep (Merino X ET cross) were infected with 300 metacercariae of Fasciola gigantica. The blood samples were collected every 2 weeks by using EDTA venoject tubes in order to determine the amount of eosinophils and the PCV value. After 14 weeks of infection all of sheep were killed and the liver was collected in order to determine the number of flukes. The results showed that the amount of eosinophils increased 2 weeks after infection and reached the peak at week 4 after infection. The average of eosinophils in ET appeared higher than the other 2 breeds (Merino was the lowest and the backcross was in between). The correlation between the number of flukes recovered from the liver and the eosinophil counts were positive in ET and Merino, but negative in the backcross sheep. The PCV values remained constant along the trial, except at week 14 after infection; the PCV values were slightly decreased in backcross sheep and Merino sheep, but not in ET sheep. The correlation between number of flukes in the liver and the PCV values were negative in all breeds of sheep. These results suggested that the eosinophilic and PCV’s response of ET were higher compared to backcross and Merino sheep, thus that responses were thought to be associated with the resistant phenomenon.   Key words: Fasciolosis, eosinophil, PCV, sheep
The effect of the liver fluke Fasciola gigantica infestation on the leucocyte eosinophil cell profile on sheep Widjajanti, S; Estuningsih, S.E; ., Subandriyo; Piedrafita, D; Raadsma, H.W
Indonesian Journal of Animal and Veterinary Sciences Vol 9, No 3 (2004)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v9i3.408

Abstract

Eosinophil is one of the major leucocyte cell in the blood which specifically reacted on parasite infection, thus it is important to determine its profile against the F. gigantica infection. The aims of this study is to determine the differences of the eosinophil count profiles on the different breed of sheep infected with F. gigantica and its relation with the resistance of sheep bred against parasitic disease. Four groups of sheep consist of Indonesian Thin Tail (ITT) sheep, Merino sheep, backcross sheep (10 families) and F2 sheep were infected with 300 metacercariae of Fasciola gigantica. The total sheep used in this trial is 621. Those sheep were observed for 12 weeks and the blood samples were collected every 2 weeks after infection. The results showed that total eosinophil counts in all infected sheep increased after two weeks post infection and ITT sheep showed the highest counts. On the other hand, the mean fluke counts on ITT sheep is the lowest compared with the other groups of sheep. Merino and F2 sheep had the highest mean fluke counts. Three families of backcross sheep had the mean flukes count similar to ITT sheep and the other 7 families were similar to the Merino sheep. In conclusion, the highest total eosinophil count at the early stage of infection on ITT sheep might be related with the genetic resistance, which was showed by the lowest flukes count, and the resistance was inherited to some of the backcross sheep, which had similar flukes count with ITT sheep.   Key words: Fasciola gigantica, eosinophil, sheep
In vitro studies: The role of immunological cells in Indonesian thin tail sheep in the killing of the liver fluke, Fasciola Estuningsih, S.E; Wiidjajanti, S; Partoutomo, S; ., Spithill; Raadsma, H; Piedrafita, D
Indonesian Journal of Animal and Veterinary Sciences Vol 7, No 2 (2002)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v7i2.284

Abstract

Previous studies have shown that Indonesian Thin Tail (ET) sheep exhibit high resistance to challenge with Fasciola gigantica when compared with Merino sheep, and this resistance is expressed in early infection. In order to study the role of the immune system in this resistance to ET sheep, in vitro studies were undertaken in the laboratory. In vitro study to confirm the ability of immune cells from ET sheep in the killing of F. gigantica larvae has been done by incubating immune cells and F. gigantica larvae together with immune sera or normal sera. The viability of the larvae was observed over a period 3 days incubation by observing their motility. The results showed that the cells isolated from F. gigantica- challenged ET sheep in the presence of immune sera from ET were able to kill 70% of the larvae. In contrast, cells from infected Merino were unable to kill a significant number of F. gigantica using the same sera source. It seems that the cytotoxicity was dependent on the presence of immune sera and ET peritoneal cells, suggesting the potential role of an antibody-dependent cell cytotoxic (ADCC) mechanism in the resistant ET sheep. Key words: In vitro, Fasciola gigantica, peritoneal cell, sheep gigantica.
The responses of eosinophil and packed cell volume (PCV) on sheep infected with Fasciola gigantica Widjajanti, S; Estuningsih, S Endah; Partoutomo, Subandriyo; Raadsma, H.W; Spithill, T.W; Piedrafita, D
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 3 (2002): SEPTEMBER 2002
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v7i3.295

Abstract

The responses of eosinophil and packed cell volume (PCV) values were verified in infected sheep, in order to identify whether these parameters could be used to predict the flukes burden and their correlation with breed resistance. Fifteen Indonesian thin tail sheep (ET), 9 Merino sheep and 148 backcross sheep generated from mating of Merino sheep and F1 sheep (Merino X ET cross) were infected with 300 metacercariae of Fasciola gigantica. The blood samples were collected every 2 weeks by using EDTA venoject tubes in order to determine the amount of eosinophils and the PCV value. After 14 weeks of infection all of sheep were killed and the liver was collected in order to determine the number of flukes. The results showed that the amount of eosinophils increased 2 weeks after infection and reached the peak at week 4 after infection. The average of eosinophils in ET appeared higher than the other 2 breeds (Merino was the lowest and the backcross was in between). The correlation between the number of flukes recovered from the liver and the eosinophil counts were positive in ET and Merino, but negative in the backcross sheep. The PCV values remained constant along the trial, except at week 14 after infection; the PCV values were slightly decreased in backcross sheep and Merino sheep, but not in ET sheep. The correlation between number of flukes in the liver and the PCV values were negative in all breeds of sheep. These results suggested that the eosinophilic and PCV’s response of ET were higher compared to backcross and Merino sheep, thus that responses were thought to be associated with the resistant phenomenon.   Key words: Fasciolosis, eosinophil, PCV, sheep
The effect of the liver fluke Fasciola gigantica infestation on the leucocyte eosinophil cell profile on sheep Widjajanti, S; Estuningsih, S.E; ., Subandriyo; Piedrafita, D; Raadsma, H.W
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 3 (2004): SEPTEMBER 2004
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v9i3.408

Abstract

Eosinophil is one of the major leucocyte cell in the blood which specifically reacted on parasite infection, thus it is important to determine its profile against the F. gigantica infection. The aims of this study is to determine the differences of the eosinophil count profiles on the different breed of sheep infected with F. gigantica and its relation with the resistance of sheep bred against parasitic disease. Four groups of sheep consist of Indonesian Thin Tail (ITT) sheep, Merino sheep, backcross sheep (10 families) and F2 sheep were infected with 300 metacercariae of Fasciola gigantica. The total sheep used in this trial is 621. Those sheep were observed for 12 weeks and the blood samples were collected every 2 weeks after infection. The results showed that total eosinophil counts in all infected sheep increased after two weeks post infection and ITT sheep showed the highest counts. On the other hand, the mean fluke counts on ITT sheep is the lowest compared with the other groups of sheep. Merino and F2 sheep had the highest mean fluke counts. Three families of backcross sheep had the mean flukes count similar to ITT sheep and the other 7 families were similar to the Merino sheep. In conclusion, the highest total eosinophil count at the early stage of infection on ITT sheep might be related with the genetic resistance, which was showed by the lowest flukes count, and the resistance was inherited to some of the backcross sheep, which had similar flukes count with ITT sheep.   Key words: Fasciola gigantica, eosinophil, sheep
In vitro studies: The role of immunological cells in Indonesian thin tail sheep in the killing of the liver fluke, Fasciola Estuningsih, S.E; Wiidjajanti, S; Partoutomo, S; ., Spithill; Raadsma, H; Piedrafita, D
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 2 (2002): JUNE 2002
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v7i2.284

Abstract

Previous studies have shown that Indonesian Thin Tail (ET) sheep exhibit high resistance to challenge with Fasciola gigantica when compared with Merino sheep, and this resistance is expressed in early infection. In order to study the role of the immune system in this resistance to ET sheep, in vitro studies were undertaken in the laboratory. In vitro study to confirm the ability of immune cells from ET sheep in the killing of F. gigantica larvae has been done by incubating immune cells and F. gigantica larvae together with immune sera or normal sera. The viability of the larvae was observed over a period 3 days incubation by observing their motility. The results showed that the cells isolated from F. gigantica- challenged ET sheep in the presence of immune sera from ET were able to kill 70% of the larvae. In contrast, cells from infected Merino were unable to kill a significant number of F. gigantica using the same sera source. It seems that the cytotoxicity was dependent on the presence of immune sera and ET peritoneal cells, suggesting the potential role of an antibody-dependent cell cytotoxic (ADCC) mechanism in the resistant ET sheep. Key words: In vitro, Fasciola gigantica, peritoneal cell, sheep gigantica.
The effect of the liver fluke Fasciola gigantica infestation on the leucocyte eosinophil cell profile on sheep Widjajanti, S; Estuningsih, S.E; ., Subandriyo; Piedrafita, D; Raadsma, H.W
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 3 (2004): SEPTEMBER 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.528 KB) | DOI: 10.14334/jitv.v9i3.408

Abstract

Eosinophil is one of the major leucocyte cell in the blood which specifically reacted on parasite infection, thus it is important to determine its profile against the F. gigantica infection. The aims of this study is to determine the differences of the eosinophil count profiles on the different breed of sheep infected with F. gigantica and its relation with the resistance of sheep bred against parasitic disease. Four groups of sheep consist of Indonesian Thin Tail (ITT) sheep, Merino sheep, backcross sheep (10 families) and F2 sheep were infected with 300 metacercariae of Fasciola gigantica. The total sheep used in this trial is 621. Those sheep were observed for 12 weeks and the blood samples were collected every 2 weeks after infection. The results showed that total eosinophil counts in all infected sheep increased after two weeks post infection and ITT sheep showed the highest counts. On the other hand, the mean fluke counts on ITT sheep is the lowest compared with the other groups of sheep. Merino and F2 sheep had the highest mean fluke counts. Three families of backcross sheep had the mean flukes count similar to ITT sheep and the other 7 families were similar to the Merino sheep. In conclusion, the highest total eosinophil count at the early stage of infection on ITT sheep might be related with the genetic resistance, which was showed by the lowest flukes count, and the resistance was inherited to some of the backcross sheep, which had similar flukes count with ITT sheep.   Key words: Fasciola gigantica, eosinophil, sheep
The responses of eosinophil and packed cell volume (PCV) on sheep infected with Fasciola gigantica Widjajanti, S; Estuningsih, S Endah; Partoutomo, Subandriyo; Raadsma, H.W; Spithill, T.W; Piedrafita, D
Indonesian Journal of Animal and Veterinary Sciences Vol 7, No 3 (2002): SEPTEMBER 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v7i3.295

Abstract

The responses of eosinophil and packed cell volume (PCV) values were verified in infected sheep, in order to identify whether these parameters could be used to predict the flukes burden and their correlation with breed resistance. Fifteen Indonesian thin tail sheep (ET), 9 Merino sheep and 148 backcross sheep generated from mating of Merino sheep and F1 sheep (Merino X ET cross) were infected with 300 metacercariae of Fasciola gigantica. The blood samples were collected every 2 weeks by using EDTA venoject tubes in order to determine the amount of eosinophils and the PCV value. After 14 weeks of infection all of sheep were killed and the liver was collected in order to determine the number of flukes. The results showed that the amount of eosinophils increased 2 weeks after infection and reached the peak at week 4 after infection. The average of eosinophils in ET appeared higher than the other 2 breeds (Merino was the lowest and the backcross was in between). The correlation between the number of flukes recovered from the liver and the eosinophil counts were positive in ET and Merino, but negative in the backcross sheep. The PCV values remained constant along the trial, except at week 14 after infection; the PCV values were slightly decreased in backcross sheep and Merino sheep, but not in ET sheep. The correlation between number of flukes in the liver and the PCV values were negative in all breeds of sheep. These results suggested that the eosinophilic and PCV?s response of ET were higher compared to backcross and Merino sheep, thus that responses were thought to be associated with the resistant phenomenon.   Key words: Fasciolosis, eosinophil, PCV, sheep