MADE PHARMAWATI
Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana, Kampus Bukit Jimbaran, Bali, Indonesia

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Journal : SIMBIOSIS Journal of Biological Sciences

EKSTRAKSI DNA DARI HERBARIUM ANGGREK NURKAMILA, UUL SHOVI; PHARMAWATI, MADE
SIMBIOSIS Journal of Biological Sciences Vol II, No 1, Tahun 2014
Publisher : SIMBIOSIS Journal of Biological Sciences

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Abstract

DNA extraction is the first step to study plant systematic and biodiversity analysis usingmolecular markers. This study aimed to conduct DNA extraction from herbarium materialsusing different extraction methods. A total of 0.05 grams of herbarium powders ofCalantheemarginata (Blume) Lindl. and Goodyera procera(Ker-Gawl) Hook. (terrestrialorchid) were used for samples by three different methods. The first method was from Doyleand Doyle with modification of incubation time for 1,5 hours at 65oC and increasing EDTAconcentration to 50 mM. Second method was Dellaporta et al. with modification of incubationtime for 1,5 hours (at 65oC) and increasing EDTA concentration to 100 mM. Third methodwas Rogers and Bendich with modification of incubation time for 1,5 hours (65oC) andadding ethanol twice. The results of electrophoresis revealed that method of Doyle and Doyleobtained DNA from C. emarginata herbarium, while method from Rogers and Bendich,unfortunately it was inconsistent. The method from Dellaporta et al.obtained DNA from G.procera herbarium, while method from Doyle and Doyle revealed inconsistent DNA forG.procera. PCR-RAPD revealed the quality of DNA isolated using Doyle and Doyle methodwas not optimal, showed by unclear patterns of DNA bands. PCR-RAPD using DNA isolatedwith method from Rogers and Bendich revealed clearer DNA bands but only for small sizefragment.Keywords : orchid, DNA extraction, herbarium, PCR
KERUSAKANKROMOSOM BAWANG MERAH (Allium cepaL.) AKIBAT PERENDAMAN DENGAN ETIDIUM BROMIDA Fibayani Imaniar, Eka; Pharmawati, Made
SIMBIOSIS Journal of Biological Sciences Vol II No.2 , Tahun 2014
Publisher : SIMBIOSIS Journal of Biological Sciences

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Abstract

The aim of this research was to identify thedamage of onion’s(AlliumcepaL.) chromosomes causedbyethidiumbromide submersion for 6and12hoursat 500ppm. The methodused to study chromosome damage of onionroot tip wassquash technique. The result showed several types of chromosomedamagesuch as the formation of, micronuclei, nuclear buds and chromosome bridges. At 6 hours submersion,the average percentageof chromosomal damage was 2.99 %, while in submersion for 12 hours, the average percentage of chromosomal damage was 6.81 %. Keywords:Ethidium bromide, chromosome damage, Allium cepa L.
PENGAMATAN MORFOLOGI DAN ANATOMI BIBIT KAMBOJA JEPANG (Adenium sp.) AKIBAT PERENDAMAN BIJI DENGAN KOLKISIN Yunita Putri Aryani, Putu; Pharmawati, Made
SIMBIOSIS Journal of Biological Sciences Vol 3, No 2 (2015): Vol III. No. 2 2015
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This research aimed to determine the effect of colchicineby seed immersion ondessert rose (Adenium sp.) seedling. Observation were done on morphological and anatomical characters. This research was conducted using colchicine concentration of 0%, 0.05%, 0.1%, and 0.15%. Each treatment had 10 replications. The parameters observed included seedling emergence, seedling height, number of leaves, leaf length, leaf width, and stomatal density of cotiledone. The results showed differences in morphological characters, led to the emergence of seeds on the ground inhibited by immersion in colchicine. Anatomically giving of colchicines cause a reduction instomatal density of cotiledone. Keywords: anatomy, colchicine, dessert rose, morphology
JENIS-JENIS LAMUN DI PANTAI LEMBONGAN, NUSA LEMBONGAN DAN ANALISISNYA DENGAN PCR RUAS rbcL Kurnia, Maliza; Pharmawati, Made; Yusup, Deny S.
SIMBIOSIS Journal of Biological Sciences Vol 3, No 2 (2015): Vol III. No. 2 2015
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Seagrasses in Bali are distributed on coastal areas of south east Bali coastal waters of Nusa Dua, Serangan Island,Sanur Beach and beaches in Nusa Lembongan. In Bali, it has been reported that there are eight species of seagrasses. Thisresearch aimed to identify seagrass species on Lembongan Beach based on morphological characters and optimize PCRcondition for molecular analysis. This research is a preliminary research on molecular method for seagrass analysis. Seagrasssampling was conducted in Lembongan Beach (in front of Ketut’s Losmen) in Nusa Lembongan, Nusa Penida Subdistrict,Bali Province. DNA extraction method used was the method of Doyle and Doyle with modifications. Result showed thatthere are five species found in Lembongan Beach, Nusa Lembongan. These seagrass are Cymodocea rotundata, Enhalusacoroides, Halodule pinifolia, Thalassodendron ciliatum and Thalassia hemprichii. DNA extraction resulted in high size ofDNA and smear DNA. Optimation of PCR reaction of rbcL fragment was done at DNA concentration of 30 ng and 50 ng.The electrophoresisof PCR products showed that DNA concentration of 50 ng had thicker band than concentration of 30 ng.Keywords: DNA extraction, Morphology, PCR rbcL, Seagrass
PEMILIHAN PRIMER RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) PADA PCR (POLYMERASE CHAIN REACTION) TANAMAN KAMBOJA (Plumeria sp.) Vanesa Martida, Vanesa; Pharmawati, Made
SIMBIOSIS Journal of Biological Sciences Vol 4, No 1 (2016)
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Abstract

There are many variation of Plumeria sp. that grown in Bali. The genetic identity of Plumeria sp. need to be analysedusing molecular study for plant breeding purpose. DNA extraction and primer selection are basic steps for molecular studyespecially in identification and analysis of genetic diversity. The aim of this research was to determine RAPD primerssuitable for molecular analysis of Plumeria sp. This research used CTAB method with modification for DNA extraction. Thesamples were young leaves of Plumeria sp. dried using silica gel. The primers used were produced by University of BritishColumbia and Operon Primer Technology. The results showed that DNA concentration of Plumeria sp from dried leaves wasbetween 33-267 ng/?l. Out of seven primers tested, three primers UBC-127, UBC-250, and OPH-06 produced clear andscorable amplification products for further analyses.Keywords: DNA, Plumeria sp., RAPD primer
PEMILIHAN PRIMER RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) PADA PCR (POLYMERASE CHAIN REACTION) TANAMAN KAMBOJA (Plumeria sp.) Martida, Vanesa; Pharmawati, Made
SIMBIOSIS Journal of Biological Sciences Vol 4, No 1 (2016)
Publisher : SIMBIOSIS Journal of Biological Sciences

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Abstract

There are many variation of Plumeria sp. that grown in Bali. The genetic identity of Plumeria sp. need to be analysedusing molecular study for plant breeding purpose. DNA extraction and primer selection are basic steps for molecular studyespecially in identification and analysis of genetic diversity. The aim of this research was to determine RAPD primerssuitable for molecular analysis of Plumeria sp. This research used CTAB method with modification for DNA extraction. Thesamples were young leaves of Plumeria sp. dried using silica gel. The primers used were produced by University of BritishColumbia and Operon Primer Technology. The results showed that DNA concentration of Plumeria sp from dried leaves wasbetween 33-267 ng/?l. Out of seven primers tested, three primers UBC-127, UBC-250, and OPH-06 produced clear andscorable amplification products for further analyses.Keywords: DNA, Plumeria sp., RAPD primer