MADE PHARMAWATI
Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana, Kampus Bukit Jimbaran, Bali, Indonesia

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INDUKSI MUTASI TANAMAN CABAI MERAH (CAPSICUM ANNUUM L.) DENGAN ETHYL METHANESULFONATE PADA BERBAGAI TINGKAT WAKTU PERENDAMAN WIARTANA, I MADE AGUS; PHARMAWATI, MADE; SUADA, I KETUT
Agrotrop: Journal on Agriculture Science Vol 4 No 1 (2014)
Publisher : Fakultas Pertanian Universitas Udayana

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Induction of Mutation of Red Chili (Capsicum Annuum L.) Using Ethyl Methanesulfonate at Several Soaking Periods.One way to increase genetic variation is through induced mutation usingchemical mutagen. Ethyl methanesulfonate (EMS) is a chemical compound that can cause mutationsand commonly used in plant. In this study seeds of red chili were treated using EMS 1% through seedsoaking. Seeds of red chili were soaked with EMS 1% in phosphate buffer pH 7 for 6, 9, 12 and 15hours at room temperature. As control, seeds were soaked in phosphate buffer pH 7. This study aimsto evaluate, physiological and reproductive characters of plants after treated with EMS. Experimentwas conducted in an open field with 5 replicates for each treatment. Results showed that concentrationof chlorophyll a, b and total chlorophyll increased in plants derived from seed treated with EMS 1% for9 hours compared to control and other soaking periods. Soaking seeds with EMS 1% for 12 hoursincreased viability of pollen compared to control and other treatments. The first time of floweringoccurred earlier at 6 and 9 hours soaking period.
PENGARUH ETHYL METHANE SULPHONATE (EMS) TERHADAP PERTUMBUHAN DAN VARIASI TANAMAN MARIGOLD (TAGETES SP.) PRATIWI, NI MADE DIAN; PHARMAWATI, MADE; ASTARINI, IDA AYU
Agrotrop: Journal on Agriculture Science Vol 3 No 1 (2013)
Publisher : Fakultas Pertanian Universitas Udayana

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The Effect of Ethyl Methane Sulphonate (EMS) on Growth and Variations of Marigold (Tagetes sp.) The aims of this research are to determine the variation of marigold (Tagetes sp) derived from seed treated with EMS and to recommend the EMS concentrations that are able to induce varietion. Seeds of marigold cv Narai Orange were soaked in water for 6 hours, followed by soaking in EMS at concentration of 0%, 0.3%, 0.6% and 0.9% for 4 hours. This study employed Randomized Complete Blok Design with 10 replicates and each replicate consisted of 10 plants. Six plants were randomly chosen for measurements. The total number of samples observed were 240 plants. Observations were made on the percentage of the growth, plant height, number of leaves, number of branches, diameter and weight of flowers. Data obtained from the observations were analyzed using Analysis of Variance (ANOVA), followed by DMRT (Duncan?s Multiple Range Test) if there is a significant difference between treatments. The EMS treatment reduced all characters observed. The EMS concentration of 0.6% showed plant that had yellow flowers. The 0.9% EMS treatment resulted in one plant with chimera, 6 dwarf plants, 2 plants with thin stems, and 1 short plant with many branches. Untreated plants did not show any variation.
Aksi Ethyl Methane Sulphonate terhadap Munculnya Bibit dan Pertumbuhan Cabai Rawit(Capsicum frutescens L.) (Ethyl Methane Sulphonate Action on Seed Emergence and Growth of (Capsicum frutecens L.)) Rustini, Ni Kadek Dewi; Pharmawati, Made
JURNAL BIOS LOGOS Vol 4, No 1 (2014): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.4.1.2014.4836

Abstract

Abstrak Penelitian ini bertujuan mengamati pengaruh lama perendaman biji dengan 1% EMS terhadap persentase munculnya bibit dan karakter pertumbuhan tanaman cabai rawit pada umur 4 minggu setelah tanam (MST). Benih cabai rawit direndam dalam air selama 6 jam, selanjutnya direndam 1% EMS dalam buffer fosfat pH 7, selama 6 jam, 9 jam dan 12 jam. Tiap biji disemai dalam bumbungan kertas. Munculnya bibit diamati setiap hari. Setelah berumur 3 minggu, bibit dipindahkan ke bedengan. Hasil menunjukkan perlakuan 1% EMS memperlambat munculnya bibit. Pada 10 hari setelah semai (HSS) munculnya bibit pada perlakuan dapat mencapai 100%. Perlakuan 1% EMS berpengaruh nyata terhadap tinggi tanaman dan jumlah daun, sedangkan panjang, dan lebar daun dengan perendaman 6 jam tidak berbeda dengan kontrol, namun berbeda dengan perendaman 9 jam dan 12 jam. Kata kunci : EMS, bibit, pertumbuhan, Capsicum frustescens L.   Abstract This research aimed to evaluate the influence of different exposure durations of 1% EMS on seedling emergence and growth characters of C, fustescent at 4 week after planting (WAT). Seeds were soaked in water for 6 hours, then soaked in 1% EMS in phosphate buffer pH 7, for 6 hours, 9 hours and 12 hours. Each seed was then sowed in a single paper tube. Seedling emergence was observed every day. At 3 weeks after sowing, seedlings were transferred to field. Results showed that soaking seeds in 1% EMS inhibited seedling emergence. At 10 days after sowing, the percentages of seedling emergence at control and treated seed were 100%. Treatments of 1% EMS have a significant effect on plant height, and the number of leaf, while length, and width of leaf with 6 hour exposure were not different with control, but differ from exposure of 9 hours and 12 hours. Keywords: EMS, seed emergence, growth, Capsicum frutescens L.
EKSTRAKSI DNA DARI HERBARIUM ANGGREK NURKAMILA, UUL SHOVI; PHARMAWATI, MADE
SIMBIOSIS Journal of Biological Sciences Vol II, No 1, Tahun 2014
Publisher : SIMBIOSIS Journal of Biological Sciences

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DNA extraction is the first step to study plant systematic and biodiversity analysis usingmolecular markers. This study aimed to conduct DNA extraction from herbarium materialsusing different extraction methods. A total of 0.05 grams of herbarium powders ofCalantheemarginata (Blume) Lindl. and Goodyera procera(Ker-Gawl) Hook. (terrestrialorchid) were used for samples by three different methods. The first method was from Doyleand Doyle with modification of incubation time for 1,5 hours at 65oC and increasing EDTAconcentration to 50 mM. Second method was Dellaporta et al. with modification of incubationtime for 1,5 hours (at 65oC) and increasing EDTA concentration to 100 mM. Third methodwas Rogers and Bendich with modification of incubation time for 1,5 hours (65oC) andadding ethanol twice. The results of electrophoresis revealed that method of Doyle and Doyleobtained DNA from C. emarginata herbarium, while method from Rogers and Bendich,unfortunately it was inconsistent. The method from Dellaporta et al.obtained DNA from G.procera herbarium, while method from Doyle and Doyle revealed inconsistent DNA forG.procera. PCR-RAPD revealed the quality of DNA isolated using Doyle and Doyle methodwas not optimal, showed by unclear patterns of DNA bands. PCR-RAPD using DNA isolatedwith method from Rogers and Bendich revealed clearer DNA bands but only for small sizefragment.Keywords : orchid, DNA extraction, herbarium, PCR
KERUSAKANKROMOSOM BAWANG MERAH (Allium cepaL.) AKIBAT PERENDAMAN DENGAN ETIDIUM BROMIDA Fibayani Imaniar, Eka; Pharmawati, Made
SIMBIOSIS Journal of Biological Sciences Vol II No.2 , Tahun 2014
Publisher : SIMBIOSIS Journal of Biological Sciences

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The aim of this research was to identify thedamage of onion’s(AlliumcepaL.) chromosomes causedbyethidiumbromide submersion for 6and12hoursat 500ppm. The methodused to study chromosome damage of onionroot tip wassquash technique. The result showed several types of chromosomedamagesuch as the formation of, micronuclei, nuclear buds and chromosome bridges. At 6 hours submersion,the average percentageof chromosomal damage was 2.99 %, while in submersion for 12 hours, the average percentage of chromosomal damage was 6.81 %. Keywords:Ethidium bromide, chromosome damage, Allium cepa L.
PENGAMATAN MORFOLOGI DAN ANATOMI BIBIT KAMBOJA JEPANG (Adenium sp.) AKIBAT PERENDAMAN BIJI DENGAN KOLKISIN Yunita Putri Aryani, Putu; Pharmawati, Made
SIMBIOSIS Journal of Biological Sciences Vol 3, No 2 (2015): Vol III. No. 2 2015
Publisher : SIMBIOSIS Journal of Biological Sciences

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This research aimed to determine the effect of colchicineby seed immersion ondessert rose (Adenium sp.) seedling. Observation were done on morphological and anatomical characters. This research was conducted using colchicine concentration of 0%, 0.05%, 0.1%, and 0.15%. Each treatment had 10 replications. The parameters observed included seedling emergence, seedling height, number of leaves, leaf length, leaf width, and stomatal density of cotiledone. The results showed differences in morphological characters, led to the emergence of seeds on the ground inhibited by immersion in colchicine. Anatomically giving of colchicines cause a reduction instomatal density of cotiledone. Keywords: anatomy, colchicine, dessert rose, morphology
JENIS-JENIS LAMUN DI PANTAI LEMBONGAN, NUSA LEMBONGAN DAN ANALISISNYA DENGAN PCR RUAS rbcL Kurnia, Maliza; Pharmawati, Made; Yusup, Deny S.
SIMBIOSIS Journal of Biological Sciences Vol 3, No 2 (2015): Vol III. No. 2 2015
Publisher : SIMBIOSIS Journal of Biological Sciences

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Seagrasses in Bali are distributed on coastal areas of south east Bali coastal waters of Nusa Dua, Serangan Island,Sanur Beach and beaches in Nusa Lembongan. In Bali, it has been reported that there are eight species of seagrasses. Thisresearch aimed to identify seagrass species on Lembongan Beach based on morphological characters and optimize PCRcondition for molecular analysis. This research is a preliminary research on molecular method for seagrass analysis. Seagrasssampling was conducted in Lembongan Beach (in front of Ketut’s Losmen) in Nusa Lembongan, Nusa Penida Subdistrict,Bali Province. DNA extraction method used was the method of Doyle and Doyle with modifications. Result showed thatthere are five species found in Lembongan Beach, Nusa Lembongan. These seagrass are Cymodocea rotundata, Enhalusacoroides, Halodule pinifolia, Thalassodendron ciliatum and Thalassia hemprichii. DNA extraction resulted in high size ofDNA and smear DNA. Optimation of PCR reaction of rbcL fragment was done at DNA concentration of 30 ng and 50 ng.The electrophoresisof PCR products showed that DNA concentration of 50 ng had thicker band than concentration of 30 ng.Keywords: DNA extraction, Morphology, PCR rbcL, Seagrass
Optimasi Konsentrasi DNA dan MgCl2 pada Reaksi Polymerase Chain Reaction-Random Amplified Polymorphic DNA untuk Analisis Keragaman Genetik Tanaman Faloak (Sterculia quadrifida R.Br) (Optimization of DNA and MgCl2 Concentrations in Polymerase Chain Reacti Uslan, Uslan; Pharmawati, Made
JURNAL BIOS LOGOS Vol 5, No 1 (2015): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.5.1.2015.9316

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Abstrak Faloak  merupakan tanaman yang tumbuh di lahan kritis. Sebagai upaya mendukung pemuliaan dan konservasi tanaman faloak diperlukan informasi keragaman genetiknya. Salah satu metode analisis keragaman genetik adalah menggunakan penanda DNA yang berbasis PCR. Untuk itu diperlukan kondisi PCR (Polymerase Chain Reaction) yang tepat sehingga diperoleh hasil yang dapat dianalisis lebih lanjut. Penelitian ini bertujuan menentukan kondisi optimum PCR-RAPD (Polymerase Chain Reaction-Random Amplified Polymorphic DNA) tanaman faloak. Ekstraksi DNA dilakukan dengan metode CTAB. Optimasi dilakukan dengan menggunakan beberapa konsentrasi DNA cetakan dan MgCl2. Kondisi optimum PCR-RAPD tanaman faloak yang menghasilkan pita produk PCR yang jelas diperoleh  menggunakan 50 ng/ul DNA, 3 mM MgCl2 serta jumlah siklus termal 45 x. Kata kunci : PCR-RAPD, optimasi, tanaman faloak Abstract Faloak is a plant that grows on critical lands. In an effort to support breeding and conservation of faloak, information about its genetic diversity is required. One of the methods of genetic diversity analysis is using PCR-based DNA markers. For that purpose, proper PCR conditions is needed in order to obtain results that can be further analyzed. This study aimed to determine the optimum conditions for PCR-RAPD of faloak plants. DNA extraction was conducted using CTAB. Optimization was done by using several concentrations of DNA templates and MgCl2. The optimum conditions of PCR-RAPD of faloak plants that produce clear band of PCR products were obtained using 50 ng/ ul DNA, 3 mM MgCl2 and 45x thermal cycles Keywords : PCR-RAPD, optimization, faloak plant
PEMILIHAN PRIMER RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) PADA PCR (POLYMERASE CHAIN REACTION) TANAMAN KAMBOJA (Plumeria sp.) Vanesa Martida, Vanesa; Pharmawati, Made
SIMBIOSIS Journal of Biological Sciences Vol 4, No 1 (2016)
Publisher : SIMBIOSIS Journal of Biological Sciences

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There are many variation of Plumeria sp. that grown in Bali. The genetic identity of Plumeria sp. need to be analysedusing molecular study for plant breeding purpose. DNA extraction and primer selection are basic steps for molecular studyespecially in identification and analysis of genetic diversity. The aim of this research was to determine RAPD primerssuitable for molecular analysis of Plumeria sp. This research used CTAB method with modification for DNA extraction. Thesamples were young leaves of Plumeria sp. dried using silica gel. The primers used were produced by University of BritishColumbia and Operon Primer Technology. The results showed that DNA concentration of Plumeria sp from dried leaves wasbetween 33-267 ng/?l. Out of seven primers tested, three primers UBC-127, UBC-250, and OPH-06 produced clear andscorable amplification products for further analyses.Keywords: DNA, Plumeria sp., RAPD primer
THE GENETIC RELATIONSHIPS OF GREVILLEA HYBRIDS DETERMINED BY RAPD MARKER PHARMAWATI, MADE; MACFARLANE, IAN JAMES
HAYATI Journal of Biosciences Vol. 20 No. 4 (2013): December 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (488.987 KB) | DOI: 10.4308/hjb.20.4.196-200

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Grevillea (Proteaceae) is native to Australia and is known as garden plant. The flowers have high diversity colors, which range over orange, yellow, pink, red and green. It is a large genus, consisted of 357 species. This genus is economically important and there are hundreds of Grevillea hybrids with beautiful color and shape of flower.  Information of the genetic relationships of Grevillea hybrids is not available. Ten Grevillea hybrids were used in this study to evaluate the genetic relationships between hybrids and with their parents. PCR-RAPD technique was employed in this study with seven RAPD primers. The dendrogram of Grevillea hybrids was performed using Neighbor-Joining analysis based on genetic distance. The analysis revealed that ?Moonlight?, ?Caloundra Gem?, and Little Pink Willie? were clustered with Grevillea banksii. The three hybrids were hybrid of G. banksii and other Grevillea species.  ?Superb? was closely related to ?Robyn Gordon? as they are hybrid of G. banksii and G. bipinnatifida.