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Pengaruh kombinasi bahan penyamak formalin dan syntan terhadap kualitas kulit ikan pari tersamak Sahubawa, Latif; Pertiwiningrum, Ambar; Pamungkas, Adityo Triarso
Majalah Kulit, Karet, dan Plastik Vol 27, No 1 (2011): Majalah Kulit, Karet, dan Plastik
Publisher : Center for Leather, Rubber, and Plastic Ministry of Industry, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2887.051 KB) | DOI: 10.20543/mkkp.v27i1.211

Abstract

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MICROCONTROLLER BASED HYDROGEN SULFIDE (H2S) MONITORING IN BIOGAS SYSTEM Yuniarti, Diah; -, Sarjiya; Pertiwiningrum, Ambar
ASEAN Journal of Systems Engineering Vol 1, No 1 (2013): ASEAN Journal of Systems Engineering
Publisher : Master Program of Systems Engineering

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Abstract

Energy issue, particularly related to fossil fuel, is one the main issues today. Biogas is a new and renewable energy issued in National Research Agenda (ARN) 2010-2014 to overcome the energy crisis problem. A so called purification process is used to remove contaminants in biogas, including H2S in order to improve the standard requirement of biogas application. In this research, H2S concentration in biogas system is measured and monitored by H2S monitoring system.The hardware system comprises of piping and microcontroller AVR 8535 systems while the software system comprises of codes written in Delphi 7.0 and Code Vision AVR Eval. The result of the system is displayed on LCD or computer to enable the monitoring process by operator. The monitoring of H2S sensor responses will be carried out for recycled BFA size of 60+100 mesh and -200 mesh.Experiment has been conducted for sensor warming up time and response time for H2S and CH4 sensor, calibration process for H2S sensor and filter column. Based on sensor calibration experiment, transfer function of TGS825 sensor from calibration is y = 0.0203x + 27.153. The best adsorption model which represents BFA adsorption of biogas from tofu waste in the research is Thomas model.
MODEL KERJASAMA LEMBAGA SWASTA, PEMERINTAH DAN PENDIDIKAN TINGGI Widhyharto, Derajad S; Pertiwiningrum, Ambar; Sutopo, Oki Rahadianto
Jurnal Sosiologi Reflektif Vol 7, No 2 (2013): Sosiologi Reflektif
Publisher : Universitas Islam Negeri Sunan Kalijaga Yogyakarta

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Abstract

The dynamics of development has opened the chance for every institutionsto do cooperation, especially among private, governmentand higher education institution. In reality, it is not easy to maintaincooperations between each institution. Because of different culture andnorm setting, It needs readiness and mutual understanding betweeninstitutions. This research wants to show the process of cooperationand its modification that happened because of different cultureand norm setting in each institution. As a result, transformationhappened from inclusive to exclusive. This conditions shows thatcooperation has deviate from its goal and its ideal type. Otherwise,it goes towards an absurd modification in the process of cooperationbetween institutions.
DIRECT STIMULATION BY METHANOL ADDITION ON THE CULTURED MEDIUM FOR METHANOL DEHYDROGENASE PROTEIN PURIFICATION FROM BRADYRHIZOBIUM JAPONICUM USDA110 Kurniawati, Novita; Pertiwiningrum, Ambar; Erwanto, Yuny; Fitriyanto, Nanung Agus; Abidin, Mohammad Zainal
Buletin Peternakan Vol 42, No 3 (2018): BULETIN PETERNAKAN VOL. 42 (3) AUGUST 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.794 KB) | DOI: 10.21059/buletinpeternak.v42i3.28155

Abstract

Methanol dehydrogenase (MDH) enzyme was purified from Bradyrhizobium japonicum USDA110 cell-free extract. The bacteria were grown in a culture medium with direct 0.5% methanol addition aimed to stimulates the MDH catalytic enzyme activation. Bradyrhizobium japonicum USDA110 MDH enzyme was purified by using 25 mM 2-(N-morpholine) ethanesulfonic acid/MES pH 5.5 buffer and 1 M sodium chloride/NaCl which separated into three columns, the first column was PD-10 for buffer exchange; the second column was HiTrap Sepharose HP to obtain unbonded fraction in the column; and the third column was Mono S 5/50 GL integrated with two pumps HPLC (high-performance liquid chromatography) to obtain pure MDH enzyme for serial changing of 1 M NaCl-25mM MES pH 5.5 with the flow rate at 1 ml/min. The protein concentration and MDH catalytic enzyme activity were observed on each purification process starting from the cell-free extract to pure MDH enzyme. The pure MDH enzyme was obtained by Mono S 5/50 GL-HPLC purification which showed a single band on SDS PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). The MDH enzyme purification from Bradyrhizobium japonicum USDA110 showed 90-fold purification, a sub-molecular weight of 63 kDa, specific activity at 2.69 U/mg, and optimum activity at a 35oC temperature and pH 9.     
INTEGRATED LIVESTOCK ADOPTION OF INNOVATION CASE STUDY: ARGOSARI AND ARGOREJO VILLAGE, SEDAYU DISTRICTS, BANTUL DISTRICT, D.I YOGYAKARTA PROVINCE Supriadi, Supriadi; Agus, Ali; Darwin, Muhadjir; Rijanta, Rijanta; Pertiwiningrum, Ambar
Buletin Peternakan Vol 41, No 3 (2017): BULETIN PETERNAKAN VOL. 41 (3) AGUSTUS 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (484.864 KB) | DOI: 10.21059/buletinpeternak.v41i3.22366

Abstract

 ABSTRACT Integrated farm is a model for the management of local resources? potential by integrating livestock and crops into eco-friendly farming (zero waste). It can be the answer for the country?s basic needs of food that is still dependent on imports. Most of the habitants of Argorejo and Argosari village work as farmers and ranchers. Although the formal education of rural people is still low due to low income, the average member of activities to get information, knowledge and management skills in agribusiness sectors to understand the practical, economical, and effective ways in business development. It facilitates adoption of innovation and the process of information absorption. Adoption of innovation is a process of mental or behavioral changes in the form of knowledge (cognitive), attitudes (affective), and skills (psychomotor) since one recognizes the innovation until one decides to adopt it after receiving it. This is qualitative research, with research material in the form of members of agribusiness activities in the intervention program called Mandiri Bersama Mandiri. The method used is descriptive analysis. The data is collected by interview, observation, and records related to the activities of the group. The result of Classification Analysis with Adopter type shows that 7 of the 8 groups in the facilitation of innovation adoption of integrated farms have: 6.7% to 14.3% innovator; 8.3% to 18.2% early adopter; and the rest is 71.4% to 77.8% early majority. One group has no innovator and up to 50% early adopter. The innovators of Mandiri Bersama Mandiri program include the most influential people that have important positions, such as group leaders, village officials, or persons who have knowledge. It indicates that the potential success of the intervention program is located on the adopter type that exists in each member, absorption of new innovations adoption, and succeed in the community empowerment program. 
Penyuluhan dan Pendampingan Pengolahan Limbah Peternakan Sapi Potong di Kelompok Tani Ternak Sido Mulyo Dusun Pulosari, Desa Jumoyo, Kecamatan Salam, Kabupaten Magelang Fitriyanto, Nanung Agus; Triatmojo, Suharjono; Pertiwiningrum, Ambar; Erwanto, Yuny; Abidin, Mohammad Zainal; Baliarti, Endang; Suranindyah, Yustina Yuni
Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 1, No 1 (2015): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpkm.16955

Abstract

Society services activity on cattle waste management system have been implemented in Sido Mulyo Livestock Farmers Group at Pulosari, Jumoyo, Salam, Magelang. Animal byproducts that consist of feces and urine of cattle wastewas processed into organic fertilizer compost and liquid fertilizer. Sido Mulyo Livestock Farmer Group has one unit of 20 m3 biodigester to accommodate the feces from approximately 30 cattle owned by the group member. Biogas has been used as a fuel source for family group members located around the cage. Slurry resulted from anaerobic digestion of biodigester disposed to pastures located on the right side of the cage. Ownership system in the groupis every group member hasa responsibility for taking care of their own cattle. The number of livestock owned by each member of the SidoMulyoLivestock Farmers Group ranged between 1 to 4 cattle. Society services methods that have been implemented was in the form of mentoring for a member of the Sido Mulyogroup.The other activities that have been implemented was the training and development of cattle industry, especially the handling of livestock waste in the form of feces, urine, and the feed residue. The activities was continued by the manufacture of compost packaging design, followed by the last series of activities such as monitoring and program development. The enthusiasm of the group members in joining to the extension activities is very good. The timing of the extension are determined in the afternoon after members of the group have finished searching feed for their cattle. The sustainability forwaste processing into organic fertilizer compost and liquid organic fertilizer becomes a major concern, because it is highly dependent on consumer demand.
Penyuluhan dan Pendampingan Pengolahan Limbah Peternakan Sapi Potong di Kelompok Tani Ternak Sido Mulyo Dusun Pulosari, Desa Jumoyo, Kecamatan Salam, Kabupaten Magelang Fitriyanto, Nanung Agus; Triatmojo, Suharjono; Pertiwiningrum, Ambar; Erwanto, Yuny; Abidin, Mohammad Zainal; Baliarti, Endang; Suranindyah, Yustina Yuni
Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 1, No 1 (2015): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (527.582 KB) | DOI: 10.22146/jpkm.16955

Abstract

Society services activity on cattle waste management system have been implemented in Sido Mulyo Livestock Farmers Group at Pulosari, Jumoyo, Salam, Magelang. Animal byproducts that consist of feces and urine of cattle wastewas processed into organic fertilizer compost and liquid fertilizer. Sido Mulyo Livestock Farmer Group has one unit of 20 m3 biodigester to accommodate the feces from approximately 30 cattle owned by the group member. Biogas has been used as a fuel source for family group members located around the cage. Slurry resulted from anaerobic digestion of biodigester disposed to pastures located on the right side of the cage. Ownership system in the groupis every group member hasa responsibility for taking care of their own cattle. The number of livestock owned by each member of the SidoMulyoLivestock Farmers Group ranged between 1 to 4 cattle. Society services methods that have been implemented was in the form of mentoring for a member of the Sido Mulyogroup.The other activities that have been implemented was the training and development of cattle industry, especially the handling of livestock waste in the form of feces, urine, and the feed residue. The activities was continued by the manufacture of compost packaging design, followed by the last series of activities such as monitoring and program development. The enthusiasm of the group members in joining to the extension activities is very good. The timing of the extension are determined in the afternoon after members of the group have finished searching feed for their cattle. The sustainability forwaste processing into organic fertilizer compost and liquid organic fertilizer becomes a major concern, because it is highly dependent on consumer demand.
BIOSORPTION OF METAL IONS ON METHANOL DEHYDROGENASE ENZYMATIC ACTIVITY OF BRADYRHIZOBIUM JAPONICUM USDA110 Kurniawati, Novita; Pertiwiningrum, Ambar; Erwanto, Yuny; Fitriyanto, Nanung Agus; Abidin, Mohammad Zainal
Buletin Peternakan Vol 42, No 2 (2018): BULETIN PETERNAKAN VOL. 42 (2) MAY 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (722.543 KB) | DOI: 10.21059/buletinpeternak.v42i2.26195

Abstract

This research aims to understand the effect of metal ions bioabsorption which belong on different elemental groups to the methanol dehydrogenase (MDH) enzymatic activity in nitrogen-fixing bacteria Bradyhizobium japonicum USDA 110. Ten metal ions with each have 30?M concentration were added to grow Bradyhizobium japonicum USDA 110 in 10-1 diluted nutrient medium. The MDH activity test showed a similar result between the bacteria grown in medium without metal ions addition (control) and the bacteria were grown in a calcium ion/Ca2+ added media. The highest MDH enzymatic activity was shown on the bacteria grown in a magnesium/Mg2+ added medium, which showed 0.08 (U/mg) enzymatic activities. The addition of magnesium/Mg2+ metal ion accelerates the bacterial growth by 2.6 times and MDH activity by 1.28 times compared to control. The MDH enzyme is essential, especially for bacteria which exist in the soil environment, to adapt to high methanol concentration and to support bacterial anaerobic growth capacity along with plant symbiotic process. Moreover, the MDH activity staining method could also act as pollutant indicators like metal ions and hydrocarbon derivates. This research concluded that metal ions biosorption (calcium/Ca2+ and magnesium/Mg2+) are required for bacterial cells reproduction and oxidation of single carbon chain compounds like methanol. The nitrogen-fixing symbiotic bacteria, Bradyhizobium japonicum USDA 110 showed high MDH activity after the two metal ions absorption. However, contrary results were shown on vanadium/V3+, manganese/Mn2+, iron/Fe3+, copper/Cu2+, zinc/Zn2+, and aluminum/Al3+ absorption, which showed low MDH activity and cells biomass.
Study of Household Biodigesters Purnomo, Chandra Wahyu; Pertiwiningrum, Ambar; Kurniawan, M. Prasetya
Agritech Vol 28, No 2 (2008)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6260.585 KB) | DOI: 10.22146/agritech.9863

Abstract

Tunnel or tubular type of bio-digester has been widely developed in many countries for producing a renewable clean fuel gas named biogas. This digester type only requires minimum maintenance and simple construction for ensuring its lower cost compared to others with similar capacity. Mostly, polyethylene (PE) plastic bag is used for construction materials of the tubular digesters. In this research, the tubular type has been constructed using large diameter PVC (Poly Vinyl Chloride) pipe for increasing the durability and safety. The performance of PVC digesters have been tested by measuring biogas production rate and CH concentration in the gas. COD (Chemical Oxygen Demand) and BOD (Biological Oxygen Demand) drop between inlet and outlet sludge have been measured for examining the digester ef- ficiency. Then, the performance was compared with PE plastic digesters data from several literatures which still needs some improvements for enhancing its capability and performance.
OPTIMIZATION OF THE USE OF FARM WASTE AND WATER HYACINTH FOR EARTHWORM (LUMBRICUS RUBELLUS) CULTIVATION MEDIA Agustina, Ulan Paluti; Prasetya, Agus; Pertiwiningrum, Ambar
Buletin Peternakan Vol 42, No 2 (2018): BULETIN PETERNAKAN VOL. 42 (2) MAY 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (826.531 KB) | DOI: 10.21059/buletinpeternak.v42i2.31581

Abstract

This study aimed to determine the effect of water hyacinth addition to farm waste on the number of earthworms, earthworm weight, media shrinkage, vermicompost quality, and the sustainability and sensitivity of earthworm farming. The study was prepared using a completely randomized design with two different factors, the water hyacinth (media without water hyacinth and media with 5% water hyacinth addition) and raw materials from farm waste (chicken and cow manure, and biogas sludge from chicken and cow manure), with three replications for each treatment. The results showed that the addition of water hyacinth to raw materials from farm waste had a very significant effect on the number of earthworms, earthworm weight, media shrinkage, vermicompost quality (except for P2O5 level that had no significant increase). When manure is to be added to the cultivation media, it is recommended to use cow manure, while both types of biogas sludge (from chicken and cow manure) are good for the cultivation of earthworm. Based on the study of sustainability, the use of farm waste and water hyacinth as media to raise earthworm gives a positive value in economic and environmental aspects. Therefore, it has great potential.