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EFEK KOMBINASI ARTEMISININ DAN N-acetylcysteineTERHADAP KADAR Malondialdehyde(MDA) OTAK DAN PARU MENCIT GALUR Balb/c YANG DIINFEKSI Plasmodium berghei Fitri, Loeki Enggar; Iskandar, Agustin; Permatasari, Nur; Gunawan, Joko Agus; Indrawan, Khadafi
Jurnal Kedokteran Brawijaya Vol 24, No 2 (2008)
Publisher : Fakultas Kedokteran Universitas Brawijaya

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Abstract

Cerebral and lung damage during malaria infection is believed to be caused by free radicals activitiesthat are produced during immunology process. The free radicals react with lipid component of cellular membrane which generates malondialdehyde (MDA) as its end-product. The aim of the research was to determine whether combination of artemisinin and NAC was moreeffective in decreasing cerebral and lung MDA level compared to artemisinin mono-therapy . The researchwas post-test-control-only design using 5 groups consisted of group A (negative control group), group B mice which infected with P.berghei without therapy (positive control group), group C mice which infected with P.berghei and received artemisinin mono-therapy (0.04 mg/g BW for 7 days), group D mice which infected with P.berghei and received artemisinin in combination with NAC (1 mg/g BW for 7 days) and group E mice which infected with P.berghei and received artemisinin in combination with NAC (1 mg/g BW for 3 days and tapered into ½ mg/g BW for 4 days). On the 3rd, 5th,and 7thday, 3 mice from each group were scarified and assayed for MDA level. On the 3rd day, a decreasing trend of cerebral and lung MDA level wasobserved on all treatment groups. On the 5thday, a decreasing trend of cerebral and lung MDA level wasobserved in group that received artemisinin and NAC whereas group’s that received artemisinin mono-therapy increased. Cerebral and lung MDA level of groupthat received artemisinin mono-therapy was significantly different with group that received combination of artemisinin and NAC in constant dose (p = 0.014) and with group that received combination artemisinin  and NAC in tapering dose (p = 0.004).
Karboksimetil Kitosan Menurunkan Degranulasi Mast Cell yang Diinduksi Oleh Ovalbumin Ibrahim, Mohamad Nur; Widjajanto, Edi; Permatasari, Nur; Sabarudin, Akhmad
Jurnal Kedokteran Brawijaya Vol 25, No 1 (2009)
Publisher : Fakultas Kedokteran Universitas Brawijaya

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Abstract

ABSTRACTOvalbumin is known as mast cell degranulating agent through the FCεRI aggregation. Carboxymethylchitosan (CMCs), a biocompatible cationic polymer was evaluated to reduces mast cell degranulation inducedby ovalbumin. In this experiment, wistar rats were darboxymethyl chitosanivided into 5 groups which consist of 1 control group and 4allergen groups. Among the allergen groups, 3 groups were treated with carboxymethyl chitosan/CMCs(doses : 0,25 mg, 0,50 mg and 1,00 mg) for 22 days. The result showed a significant (P<0,01) reduction inmast cell degranulation in allergen groups. This finding indicate that CMCs possesses antiinflammationactivity mediated by reducing of mast cell degranulation.Keywords : ,c mast cell degranulation
EFEK HAMBATAN EKSTRAK DAUN CEPLUKAN (Physalis minima L)TERHADAP KONTRAKTILITAS OTOT POLOS USUS HALUS TERPISAH MARMUT DENGAN STIMULASI METAKOLIN EKSOGEN Tarannita, Citra; Permatasari, Nur; Sudiarto, Sudiarto
Jurnal Kedokteran Brawijaya Vol 22, No 1 (2006)
Publisher : Fakultas Kedokteran Universitas Brawijaya

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Abstract

Physalis sp. has lots of merits as a kind of traditional medicine and one of them is its function as an alternatif treatment for diarrhea. Physalis minima leaf contains alkoloid atropine which is assumed can inhibit the contractionof guinea pig’s isolated ileum smooth muscle. This research was to know the effect of Physalis minima leaf extract toward the contraction of small intestine smooth muscle. This research was  an experimental study using “The Post Control GroupDesign”, and guinea pig’s isolated ileum as samples. The samples  consisted of five guinea pigs, each sample was given four kinds of treatment. The control was given metacholine at the  dose of 10 -5 without Physalis minima leaf extract. Before givingmetacholine at the dose of 10 -5 , the samples were given the first dosage of Physalis minima leaf extract (0.15%), the second dose (0.30%) and the third dose (0.45%) so getting four graphs for each sample. The level of contraction was recorded by using kymograph. The result showed that  the ileum contraction of the third group was inhibited (One Way ANOVA, p=0.041), and there was a relation between the increasing dosage of Physalis minima leaf extract and the decreasing ileum contraction (simple linear regression, with R=-0.608 p=0.04 and regression equation y=52.150 – 7.660x).
Pektin Dalam Tepung Kesemek Mempengaruhi Kadar Trigliserida Pada Tikus Wistar Jantan Yang Diberi Diet Aterogenik Setyaningtyas, Stefania Widya; Permatasari, Nur; Mustafa, Annasari
Amerta Nutrition Vol 1, No 1 (2017): AMERTA NUTRITION
Publisher : Universitas Airlangga

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Abstract

Background: Moderate hypertriglyceridemia is almost certainly an independent risk factor for cardiovascular disease. Pectin is a kind of soluble fiber that can be used to decrease triglyceride level and it  can be found in fruits such as persimmon. Objective: This research intens to prove the effect of soluble fiber of persimmon flour to decrease triglyceride level. Methods: The search used Post Only Control Group design. Normal diet, atherogenic diet, and atherogenic diet with various amount of dried persimmons were given to 30 subjects, male wistar rats, for 12 weeks. 1.2 g, 2.4 g, and 3.6 g persimmon flour were used as the given amount for every groups. Results: There were significant differences between atherogenic diet group and the other groups of research (p-value<0.001). Compared to normal diet group, atherogenic diet with 1.2 g and 2.4 g persimmon flour resulted unsignificant differences of triglycerides level. But, atherogenic diet with 3,6 g dried persimmon group was different significantly to normal diet groups (p-value=0.036). Both dose of persimmon flour and fat intake affect elevation of tryglyceride level up to 62.5%. Conclusion: The contribution of persimmon flour (Diospyros Kaki L. var Junggo) in inhibitation of the increase of triglyceride serum level on male wistar rats.  The most effective dose is 1.2 g, because the result of the trigliceride level was closest to normal and energy intake was not affected. ABSTRAK Latar belakang : Hipertrigliseridemia kadar sedang hampir pasti merupakan faktor risiko tersendiri untuk penyakit kardiovaskular. Pektin merupakan jenis serat larut air memiliki efek menurunkan trigliserida dan banyak terdapat dalam buah-buahan, salah satunya buah kesemek.Tujuan : Penelitian ini bertujuan menguji efek serat larut air pada tepung kesemek dalam menurunkan kadar trigliserida.Metode : Penelitian dilakukan dengan metode Post Test Only Control Group. Diet normal, diet aterogenik, diet aterogenik + kesemek dengan berbagai jumlah diberikan kepada 30 subyek penelitian, yaitu tikus wistar jantan selama 12 minggu. 1,2 g, 2,4 g, dan 3,6 g tepung kesemek digunakan sebagai intervensi untuk kelompok perlakuan.Hasil : terdapat perbedaan kadar trigliserida yang signifikan antara kelompok diet aterogenik dengan kelompok perlakuan lainnya (p=0,000). Bila dibandingkan dengan kelompok diet normal, perlakuan dengan pemberian tepung kesemek 1,2 g dan 2,4 g menghasilkan kadar trigliserida yang tidak berbeda signifikan, namun jumlah pemberian tepung kesemek 3,6 g berbeda nyata dengan diet normal (p=0,036). Pemberian tepung kesemek dan asupan lemak bersama-sama mempengaruhi pembentukan trigliserida dengan kontribusi sebesar 62,5%.Kesimpulan : Tepung kesemek (Diospyros Kaki L. Var. Junggo) terbukti dapat menghambat peningkatan kadar trigliserida serum pada tikus wistar jantan yang diberi diet aterogenik. Jumlah yang dinilai paling efektif menurunkan trigliserida adalah sebesar 1,2 g, karena pada jumlah tersebut, kadar trigliserida yang dihasilkan paling mendekati normal dan tidak mempengaruhi asupan energi.
Evaluation Anxiolytic Effect of Methanol Extract of Ceplukan Leaves (Physalis minima L.) in the Elevated Plus Maze Test through IL-6 Level Changes in Ovariectomized Rats Nurfitria, Siti; Permatasari, Nur; Ratnawati, Retty
Journal of Tropical Life Science Vol 5, No 1 (2015)
Publisher : Journal of Tropical Life Science

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Abstract

Ceplukan (Physalis minima L.) has long been used to treat various conditions in traditional medicine. This study aims to demonstrate the anxiolytic effects of Methanol Extract of Ceplukan Leaves (MECL) in the Elevated Plus Maze (EPM) test and correlate to IL-6 level of ovariectomized rat brain. Total of 24 Wistar rats were divided into six groups: one normal group, one group of 1 month ovariectomized (ovx), one group of 2 months ovx, three groups of 2 months ovx (each given with MECL 500; 1500 and 2500 mg/kg doses for 1 month). The anxiety-like behavior level was measured by EPM test. After EPM test, the brain was removed to measure level of IL-6 by ELISA. The data were processed and analyzed by one-way ANOVA and Pearson correlation. We found that the MECL-treated rats enter the opened-arm higher than the control rats. It indicates that the MECL-treated rats are less anxious than the control rats. The results also show the decreased of IL-6 level in MECL-treated rats.
PENGARUH PEMBERIAN MORFIN DOSIS LETHAL TERHADAP PERTUMBUHAN LARVA LALAT Chrysomya sp. PADA MEDIA BANGKAI TIKUS Rattus norvegicus GALUR WISTAR Fadhlurrahman, Ahmad Reza; Baskoro, Aswin D; Permatasari, Nur
MANDALA of Health Vol 6, No 1 (2013): Mandala Of Health
Publisher : Jurusan Kedokteran FK Unsoed

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Abstract

Death is the end of life. To identify unproper death on corpses, we use Post Mortem Interval (PMI). Insect evidence on the corpses can be used to estimate the Post Mortem Interval, however many factors including drugs may affect the rate of fly larva growth. Two rats with average BW 200 grams were used in this experimental study in order to analyze the influence of morphine on growth rate of fly larvae. One rat were given 120 grams of morphine orally (LD50 = 461mg/KgBW), and the other were killed by cervical dislocation and used as control. The rats were then simultaneously killed and caged individually into Chrysomya sp. cages to let the flies deposit their eggs on the rat corpses. Five developing larvae were sampled twice daily to determine the body length, weight and the growth rate until they were emerging to be adult. Result of this study showed that the length and the weight were lower and the growth of Chrysomya sp. larvae in rat corpses containing morphine were faster than control. The significant result occured at third stadium (p &lt; 0,005) This result suggested that morphine can precipitate the growth rate of fly larvae.
The Effectiveness Of Tofu Liquid Waste As A Natural Phytoestrogen For Mandibular Bone Of Ovariectomized Rats Aquina, Meilia; Permatasari, Nur
Insisiva Dental Journal Vol 1, No 1 (2012)
Publisher : Insisiva Dental Journal

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Abstract

Estrogen is a steroid compound that has many benefits for the bodys physiology.Hypoestrogenic women could have periodontitis in their mouth. Hormonereplacement therapy (HRT) is a therapy that used to reduce the disturbance causedby estrogen deficiency. However, HRT has many side effects. Theoretical studyshows that isoflavones in tofu liquid waste has an element of a steroid compound.The purpose of this study was to prove the effectiveness of isoflavones in the tofuliquid waste by examining the microscopic structure, estrogen receptor expressionand MDA levels in rat mandibular bone post-ovariectomy. This research methoduse a true experimental laboratory with randomized post test control group design.Twenty-four female wistar rats were divided randomly into six groups, consistedof a group of normal rat that does’nt ovariectomized (C1), 4 weeksovariectomized rats (C2), 8 weeks ovariectomized rats (C3) but no tofu liquidwaste was given, and 8 weeks ovariectomized rats that given tofu liquid waste atthe end of the 4th week of ovariectomy with three different doses (C4 = 1.2, C5 =6; C6 = 12 ml / kg). Histopathological slide used to see the changes resulted in anumber of osteoclasts, osteocytes, periodontal ligament width, alveolar boneheight, the amount of estrogen receptor expression and MDA levels. The resultsof statistical tests show that there is a difference in the rats between six differentgroups (ANOVA, p &lt;0.05) and there is a close relationship between the dose ofthe tofu liquid waste with all parameter. The conclusion of this study is tofuliquid waste could improve the microscopic structure, estrogen receptorexpression and MDA levels in rat mandibular bone post-ovariectomy.
EffectOf Panax Ginseng Extract For The Increased Number Of Fibroblasts Cells After Tooth Extraction Rizky Hutomo, Ferdian; Permatasari, Nur; Andari Wulan, Kartika
Insisiva Dental Journal Vol 1, No 1 (2012)
Publisher : Insisiva Dental Journal

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Abstract

The process of tooth extraction can lead to injury. One of the factors that influencethe process of wound healing after tooth extraction is the number of fibroblastcells. The content of saponins can increase the number of fibroblasts cells aftertooth extraction. Asian ginseng plants known to contain saponins (ginsenoside).The purpose of this study was to determine the effect of Asian ginseng extract(Panax ginseng extract) for the increased number of fibroblasts cells after toothextraction. The research method used is in vivo experimental design with thedesign methods Randomized Post Test Only Control Group Design. In this studyused twentty male rattus novergicus and divided into four group, consisting ofcontrol group (K1) were not given Asian ginseng extract, treatment groupsnumber one (K2) were given 25mg/Rattus novergicus/day doses of Asianginseng, treatment groups number two (K3) were given 50mg/ Rattusnovergicus/day doses of Asian ginseng, and treatment groups number three (K4)were given 75mg/Rattus novergicus/day doses of Asian ginseng. Histopathologicpreparations used for counting the number of fibroblasts cells. The results ofstatistical tests indicate there are differences in the number of fibroblast cells inRattus novergicus between the four different groups (ANOVA, p &lt;0.05) and thereis a very strong correlation between increasing doses of ginseng extract with anumber of fibroblast cells in Rattus novergicus tooth socket (Pearson, R = 0.915 p&lt;0.05). The conclusion of this study is Asian ginseng extract can increase thenumber of fibroblasts in the tooth socket after tooth extraction, it is recommendedthat research about the amount of saponin in Asian Ginseng extract
Cysteine, Malondyaldehide (MDA) and Glutathione (GSH) Levels in Marasmic Type Malnutrition Cahyani, Dwi Indah; Puryatni, Anik; Permatasari, Nur
Journal of Tropical Life Science Vol 7, No 2 (2017)
Publisher : Journal of Tropical Life Science

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Abstract

Micronutrien deficiency in severe malnutrition will reduce antioxidant capacity that needed for oxidative stress defense. Cysteine, a non-essential amino acid, is one of an important component for reduced glutha-tione (GSH). This study aims to prove the difference between the levels of cysteine, MDA and GSH levels in children with marasmic malnutrition and well-nourished children and prove whether there is a relationship between those parameters. Fiftysix patients participated in this study were grouped into two groups of sam-ples that were marasmic type malnutrition group (28 patients) and control groups that were well nourished group (28 patients). Examination begins with a complete laboratory screening, followed by examination of cysteine, MDA and GSH level. Of the 28 patients included in marasmic type malnutrition group consisting of 15 male patients (53.6%) and 13 female patients (46.4%), while the well-nourished group consisted of 13 male patients (46.4%) and 15 female patients (53.6%). The average age is 54.61±56.35 months in the group of ma-rasmic type malnutrition and 48.25±45.34 months in the well-nourished group. By using the Mann Whitney test, there were significant difference between the levels of cysteine and GSH in marasmic malnutrition and control group (p = 0.000 and p = 0.000 respectively). Spearman correlation test between cysteine and GSH levels, cysteine and MDA levels, MDA and GSH levels in marasmic malnutrition children shows no significant correlation (R = -0.206, p = 0.294; R = -0.036, p = 0.856; R = 0.210, p = 0.284 respectively). In well-nourished group Spearman correlation test between cysteine and GSH levels, cysteine and MDA levels, MDA and GSH levels also shows no significant correlation (R = -0.053, p=0.789; R = -0.146, p = 0.458; R = -0.079, p = 0.688 re-spectively). From this study it can be concluded that there are significant differences of the levels of cysteine and GSH between severe malnutrition groups compared with the control one. But this study shows no signif-icant correlation between the levels of cysteine and GSH levels in marasmic type malnutrition and well-nourished children.
EFEK HAMBATAN EKSTRAK DAUN CEPLUKAN (Physalis minima L)TERHADAP KONTRAKTILITAS OTOT POLOS USUS HALUS TERPISAH MARMUT DENGAN STIMULASI METAKOLIN EKSOGEN Tarannita, Citra; Permatasari, Nur; Sudiarto, Sudiarto
Jurnal Kedokteran Brawijaya Vol 22, No 1 (2006)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2006.022.01.4

Abstract

Physalis sp. has lots of merits as a kind of traditional medicine and one of them is its function as an alternatif treatment for?diarrhea. Physalis minima leaf contains alkoloid atropine which is assumed can inhibit the contractionof guinea pig?s?isolated ileum smooth muscle. This research was to know the effect of Physalis minima leaf extract toward the contraction?of small intestine smooth muscle. This research was ?an experimental study using ?The Post Control GroupDesign?, and?guinea pig?s isolated ileum as samples. The samples ?consisted of five guinea pigs, each sample was given four kinds of?treatment. The control was given metacholine at the ?dose of 10?-5?without Physalis minima leaf extract. Before givingmetacholine at the dose of 10?-5?, the samples were given the first dosage of Physalis minima leaf extract (0.15%), the?second dose (0.30%) and the third dose (0.45%) so getting four graphs for each sample. The level of contraction was?recorded by using kymograph. The result showed that ?the ileum contraction of the third group was inhibited (One Way?ANOVA, p=0.041), and there was a relation between the increasing dosage of Physalis minima leaf extract and the?decreasing ileum contraction (simple linear regression, with R=-0.608 p=0.04 and regression equation y=52.150 ??7.660x).