Jeanne A. Pawitan
Department of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta

Published : 30 Documents
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Stem cells and the prospect of tissue engineering Pawitan, Jeanne A.
Medical Journal of Indonesia Vol 18, No 2 (2009): April-June
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (182.437 KB) | DOI: 10.13181/mji.v18i2.559

Abstract

[No Abstract Available]
Association between presence in lectures and student knowledge gain in problem based learning: experience in Neuropsychiatry module, FMUI, International Program Pawitan, Jeanne A.; Sukmawati, Dewi
Medical Journal of Indonesia Vol 18, No 2 (2009): April-June
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.85 KB) | DOI: 10.13181/mji.v18i2.353

Abstract

Aim This study was conducted to determine the association between presence in lecture and examination scores.Methods This was a cross sectional study, conducted in the Faculty of Medicine University of Indonesia, International Class Program, from November 2007 to May 2008. The subject’s inclusion criterion was FMUI International class students enrolled in the Neuropsychiatry module in 2007/2008. we excluded students who did not attend the examinations i.e. multiple choice questions (MCQ)-1, -2, or essay. The data collected were presence in lecture and examination scores. Data analysis was done using SPSS linear regression to see the association between presence in lectures and exam scores.Results The results showed that the R and P of the association between presence in lectures and exam scores were (R= 0.121, P= 0.413), (R= 0.212, P= 0.148), (R= 0.260, P= 0.075), and (R= 0.280, P= 0.054) for MCQ1, MCQ2, essay, and mean exam scores respectively.Conclusion We failed to show the association between presence in lectures and student knowledge gain. This result might be due to the many factors discussed that were not analyzed in this study. (Med J Indones 2008; 18: 131-4)Key words: exam, MCQ, essay
Cytology technique: development of a simple spot method for cultured cell suspension Pawitan, Jeanne A.; Damayanti, Lia; Arleni, Arleni; Swantari, Ni M.
Medical Journal of Indonesia Vol 19, No 1 (2010): February
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (722.566 KB) | DOI: 10.13181/mji.v19i1.378

Abstract

Aim To develop a simple spot method to attach cultured cells in suspension on to a glass slide.Methods We compared three approaches using both conventional and special glass slide (Shandon-Polysin), either without additional fetal bovine serum (FBS), or with addition of 3 or 10 μl of FBS to a 20 μl sample (altogether there were six approaches). The slides were examined qualitatively for the background color, boundary color and intactness, and whether there were folded and detached parts. Further, for each slide, the attached intact cells were counted, and the percentage of attached intact cells per number of spotted cells was calculated. The difference in attach intact cells between different approaches was analyzed by ANOVA using SPSS 13.0 for windows.Results There were no significant difference in the percentage of attached intact cells between the six approaches (P= 0.804), though the approach using special glass slide without additional FBS (FBS final concentration 5%) yield the highest percentage of attached intact cells, showed clean background without folded parts.Conclusions We have developed a simple spot method for cultured cell suspension, and the best approach to make spot specimen is using special glass slide with 5% FBS in the cell suspension. (Med J Indones 2010; 19:26-31)Keywords: spot specimen, special glass slide, fetal bovine serum
Prospect of mesenchymal stem cells in bone tissue engineering Pawitan, Jeanne A.
Medical Journal of Indonesia Vol 21, No 1 (2012): February
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.676 KB) | DOI: 10.13181/mji.v21i1.545

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H5N1 Influenza: is it still a threat? Pawitan, Jeanne A.
Medical Journal of Indonesia Vol 19, No 2 (2010): May
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.726 KB) | DOI: 10.13181/mji.v19i2.553

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Mechanism of normal menstruation and abnormality associated with menorrhagia Pawitan, Jeanne A.
Medical Journal of Indonesia Vol 10, No 2 (2001): April-June
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (675.315 KB) | DOI: 10.13181/mji.v10i2.20

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Normal menstruation involves endometrial tissue breakdown and bleeding, followed by hemostasis and repair. Abnormality of this process at any stage may result in changes in the quantity of menstrual blood loss. When menstrual blood loss is greater than 80 ml, it is called menorrhagia. This review discuss the mechanism of normal menstruation, and factors associated with menorrhagia. Those factors are the endometrial bleeding associated factor (ebaf), the role of various cells (migratory leucocytes, macrophages, and mast cells), the role of various substances (lysosomal enzymes, prostaglandins, endothelins, growth factors and its receptors), impairment of fibrinolysis and hemostatic proces, and changes in endometrial blood flow. (Med J Indones 2001; 10: 121-6)Keywords : ebaf, prostaglandin, endothelin, fibrinolysis
Effect of adipose tissue processing procedures in culture result: a study preliminary Pawitan, Jeanne A.; Bustami, Arleni; Damayanti, Lia; Antarianto, Radiana D.; Swantari, Ni M.
Medical Journal of Indonesia Vol 20, No 1 (2011): February
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (31.938 KB) | DOI: 10.13181/mji.v20i1.420

Abstract

Background: There are various methods of processing adipose tissue before culture, depending on the adipose tissue samples. The aim of this study is to compare several modifications of culturing and sub-culturing procedures of adipose tissue to fit the condition in our laboratory.Method: This is a descriptive study that was done in the Immunology and Endocrinology Integrated Laboratory, University of Indonesia, from  October 2009 to April 2010. Three adipose tissue processing procedures, various amount of seeding and two subculture methods were compared in term of cell yield and time needed. In the first procedure, collagenase-1 digestion was done in 30minutes, cell seeding were 24,000 and 36,000 per flask; in the second procedure, collagenase-1 digestion was done in 60minutes, cell seeding were 24,000, 48,000, and 72,000 per flask; and in the third procedure, the adipose tissue remnants from the first  procedure were again digested for another 45 minutes, cell seeding were 74,000, and 148,000 per flask. Difference in subculture methods were the presence or absence of washing step.Result: Procedure 1 yielded the lowest amount of cell, and after culture, the cells grew very slow, and was contaminated before harvest of primary culture. Procedure-2 and -3 succeeded to yield primary cultures. Some of the cultures were contaminated, so that further subculture was not  applicable, and only one tissue processing procedure (procedure 2: 60 minute collagenase-1 digestion, without lysis buffer, cell seeding 48,000 and 72,000) could complete the three subcultures. Though some of the procedures could not be completed, final result could be concluded.Conclusion: In this preliminary study, 60 minute colagenase-1 digestion with intermittent shaking every 5 minutes and cell seeding around 50,000 or more, followed by subculture method without washing step gave the best result. (Med J Indones 2011; 20:15-9)Keywords: collagenase-1, primary culture, subculture, stromal-vascular fraction
Phytoestrogens beneficial effects on cardiovascular system Pawitan, Jeanne A.
Medical Journal of Indonesia Vol 11, No 2 (2002): April-June
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (461.967 KB) | DOI: 10.13181/mji.v11i2.60

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Several lines of evidence, including epidemiological, clinical trial and basic science, suggest the plausability of a causal, inverse relationship between phytoestrogens and cardiovascular disease. Phytoestrogens are naturally occurring plant compounds that have 2-phenylnaphthalene-type chemical structures similar to those of estrogens. they are simply divided into three main classes, isoflavones, lignans, and coumestans. The majority of phytoestrogens found in typical human diets are the isoflavones and lignans. This review provides an overview of the potential sources and bio-availability of the phytoestrogens particularly isoflavones, and discusses their beneficial effects on cardiovascular system, i.e. on atherosclerosis, hypercholestrolemia, arterial compliance, and blood pressure. (Med J Indones 2002; 11: 120-3)Keywords: atherosclerosis, hypercholestrolemia, arterial compliance, blood pressure
The possible role of nutritional intervention to prevent diabetic retinopathy Pawitan, Jeanne A.
Medical Journal of Indonesia Vol 17, No 4 (2008): October-December
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.09 KB) | DOI: 10.13181/mji.v17i4.562

Abstract

[No Abstract Available]
Comparison of various procedures in the processing of binucleated-lymphocytes on to glass slide to detect chromosomal breakage Pawitan, Jeanne A.; Suryono, Isnani A.; Damayanti, Lia; Purbadi, Sigit
Medical Journal of Indonesia Vol 12, No 1 (2003): January-March
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (177.322 KB) | DOI: 10.13181/mji.v12i1.83

Abstract

Binucleated lymphocytes can be screened for micronuclei to assess chromosomal damage. There are various procedures to get slides containing binucleated lymphocytes, that are different in harvesting, fixation, and slide preparation methods. Screening binucleated lymphocytes to find a micronucleus needs at least 800 cells with intact cytoplasm. This study aimed to analyze the various procedures and simplified procedures to know which procedure gave the most abundant binucleated lymphocytes with intact cytoplasm and best staining properties for the purpose of micronucleus scoring. Seven heparinized blood samples were obtained from the Dept. of Obstetrics and gynecology, Faculty of medicine, University of Indonesia, Jakarta. The 7 blood samples were subjected to 17 procedures different in harvesting (with or without washing), slide preparation (smear and spot method, and using a cytocentrifuge), and fixation methods (methanol for 1 minute, methanol brief, methanol/glacial acetic acid 3:1 or 9:1). Our results showed that fixatives containing glacial acetic acid are not suitable for micronucleus test. To generate binucleated lymphocytes with intact cytoplasm as much as possible, the procedure should be conducted without washing steps. Methanol fixation either briefly or 1 minute is preferable, and for the ease of screening cytocentrifuge preparation, followed by spot method is preferable. (Med J Indones 2003; 12: 3-7) Keywords: cytokinesis blocked, spot, smear, cytocentrifuge, fixatives