Abd. Rauf Patong
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Jl. Perintis Kemerdekaan Km 10 Tamalanrea, Makassar 90245, South Sulawesi

Published : 8 Documents
Articles

Found 4 Documents
Search
Journal : Al-Kimia

Isolasi Dan Karakterisasi Bakteri Simbion Larva Kupu-Kupu Cossus cossus Penghasil Enzim Selulase Baharuddin, Maswati; Patong, Abd. Rauf; Ahmad, Ahyar; La Nafie, Nursiah
Al-Kimia Vol 2, No 2 (2014): Desember
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (240.729 KB) | DOI: 10.24252/al-kimia.v2i2.1653

Abstract

This study was conducted to characterize bacterial symbionts of butterfly larvae Cossus cossus capable of degrading cellulose. This study successfully purify and characterize isolates originating  from the intestine (CC1 and CC2), head (CC3), middle (CC4), and tail (CC5). From a qualitative test using 0.1% congo red gained the clear zone indicates that the bacteria are able to degrade cellulose. Based on the test temperature and pH on the growth of the data obtained CC5 isolates including isolates of thermophilic bacteria, while others including mesophilic bacteria. While based pH test all isolates were able to grow well at neutral pH. Based on the data obtained growth curve maximum bacterial growth at the 24th hour. Based on morphology and physiology test obtained bacteria genus Acinotobacter, Pseudomonas, and Bacillus.
Pemanfaatan Kitin Sebagai Bahan Membran Elektroda Enzim Diamin Oksidase Untuk Biosensor Histamin Karim, Abdul; Patong, Abd. Rauf; Wahab, Abd. Wahid; Raya, Indah
Al-Kimia Vol 2, No 2 (2014): Desember
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (593.928 KB) | DOI: 10.24252/al-kimia.v2i2.1642

Abstract

This research aims to utilize isolated chitin from shrimp waste to develop histamine biosensors basedon diamine oxidase (DAO) enzyme electrode with cyclic voltammetry. DAO enzyme trapped in the chitin-cellulose acetate membranes with various comparisons were layered on the Pt electrode. Histamine will be oxidized by the DAO enzyme produces aldehydes and H2O2 which acts as an electron transfer mediator. Biosensor performance is influenced by several factors, especially the concentration and composition of electrode  membranei.Comparison of chitin-cellulose acetate used in this study were 1:1, 2:1 and 3:1. Isolated chitin from the shrimp waste is chemically obtainedrendamen of 23.6%, and characterization of electrode membrane by FTIR and cyclic voltammetry showed that the DAO enzymes electrode with chitin-cellulose acetate membrane 2:1 is the best composition.
Isolasi Dan Identifikasi Bakteri Termofil Penghasil Amilase Dari Sumber Air Panas Lejja Sulawesi Selatan Arfah, Rugaiyah A.; Patong, Abd. Rauf; Ahmad, Ahyar; Djide, M. Natsir
Al-Kimia Vol 2, No 2 (2014): Desember
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.196 KB) | DOI: 10.24252/al-kimia.v2i2.1644

Abstract

A Research isolation and identification of bacteria termofil amylase from hot springs Lejja South Sulawesi has been done. This study aims to characterize the morphological, biochemical, genus and species of bacteria producing  the enzyme amylase. The method used in this study through the stages: 1) Skrening and isolation of bacteria by means of as much as 1.0 mL of sample dilution plated on Petri dishes containing agar medium, then incubated for  20-24 hours at 50 °C, colonies of bacteria growing and has a colony morphology different character each taken 1 ose then etched into the amylolytic selective medium then incubated for 20-24 hours at 40oC and 50oC. Colonies that grew on selective media is scratched quadrant amylolytic to obtain pure isolates. Pure bacterial isolates taken 1 ose then grown in selective medium for 48 h at 50° C, bacterial isolates were grown spilled iodine solution (2% I2 and 0.2% KI) when there is a clearing zone around the colony indicated as the enzyme-producing bacterial isolates termofil amylase; 2) termofil characterization of bacterial isolates in microscopy with Gram stain; 3) isolates selected biochemical tests performed according  to the method Bergeys Manual and Systematic of Bacteriology. Results of screening and isolation of 10 bacterial isolates obtained amylase through iodine test, selected 2 isolates, 1 isolate from water samples RSAII-1B and 1 isolates from water samples mixed sediment RSSII-4B, which has a diameter of clearing zone of 5.6 cm respectively and 5.15 cm; out such characterization results of gram stain microscopy showed that the 2 isolates including gram + and shaped bacillus, the colony morphology as observed macroscopically, microscopy and  biochemical test results  obtained  RSAII isolates and isolates RSSII-1B-4B is a Bacillus sp.
Isolasi dan Implementasi Protein Bioaktif Kepah (Atactodea striata) Sebagai Bahan Obat Antibakteri Hasan, Tahirah; Patong, Abd. Rauf; Wahab, Abd. Wahid; Djide, M. Natsir
Al-Kimia Vol 2, No 2 (2014): Desember
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (270.675 KB) | DOI: 10.24252/al-kimia.v2i2.1652

Abstract

This study aimed to 1) determine the degree of saturation of ammonium sulfate right to extract and purify the bioactive protein from shells (Atactodea striata), 2) determine the fraction of active protein from shells (Atactodea striata) as a potential antibacterial. In this study used the Lowry method for determine protein concentration and agar diffusion method for antibacterial activity. Extraction of shells Atactodea striata was conducted by making use of buffer solution (0,1 M Tris-HCl of pH 8.3, 2 M NaCl, 0.01 M CaCl2, 1 % β-mercaptoethanol, and  0.5 % Triton X-100). Purification of proteins by  precipitation using ammonium sulfate at saturation level 30 %, 50 %, 70 %, and 90 %. The results showed that the protein concentration of the crude   extract is 41.6354 mg/mL. At fractionation rate of 0-90% saturation showed the highest concentration of protein found in fractions with 70% saturation level is 56.4184 mg/mL. The testing of antibacterial activity against Staphylococcus aureus showed that crude extracts and protein fractions Atactodea striata is considered effective as an antibacterial. The highest bioactivity during 24-hour incubation in protein fractions obtained by ammonium sulfate saturation level of 50% is 25.17 mm. Whereas the lowest activity was obtained at 90% saturation level is 14.05 mm. Bioactivity against Escherichia coli after incubation for 24 hours has the highest activity in the protein fraction with 30% ammonium sulfate saturation is 15.12 mm. Whereas the lowest activity was showed at 70% saturation level is 10.30 mm. After the observation was continued for 48 hours on both test bacteria, which formed a clear area becomes cloudy. It shows that the crude extract and fractions of protein tend to be bacteriostatic against Staphylococcus aureus and Escherichia coli.