Wesly Pasaribu
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Efektivitas Ekstrak Daun Pacar Air (Impatiens balsamina L.) Untuk Meningkatkan Respon Imun Non Spesifik Ikan Nila (Oreochromis niloticus) Pasaribu, Wesly; Longdong, Sammy N.J
e-Journal BUDIDAYA PERAIRAN Vol 3, No 1 (2015)
Publisher : e-Journal BUDIDAYA PERAIRAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (179.71 KB)

Abstract

The objective of this research was  to determine the most effective dose and the best induction time of  Impatiens balsamina extract in enhancing nonspesific immune response of nile tilapia (Oreochromis niloticus).  The tested fish were nile tilapia, 14-16 cm in length, and  39-42 g in weight, obtained from Balai Pengembangan dan Pembinaan Pembudidayaan Ikan (BP3I) Tateli. The  research was designed in 2x4 factorial in completely randomized design.  There were  two factors   tested  in this research, the dose  and  time.  There were four levels of dose,  A1 = 0 mg/mL extract, A2= 30 mg/mL extract, A3= 50 mg/mL extract, A4= 70 mg/mL extract; and there were  two levels of time,  B1= 7 days after injection and B2=14 days after injection.   The extract was injected intramuscularly  with a dose of  0.2 mL per fish.   Data collected in this research was the immune parameters (total leucocyte count and phagocytosis activity). The results showed that the most effective dose in enhancing nonspesific immune response was   A1=50 mg/mL extract and the best induction time was  B1= 7 day after injection.   The results also indicated  that there was significant  interaction between  dose and time in influencing  the total amount of leucocyte, but there was not  interaction in influencing the phagocytic activities. Keywords : Impatiens balsamina, immune Response, total leucocyte count, phagocytosis activity.
Seaweed extract of Gracilaria verrucosa as an antibacterial and treatment against Vibrio harveyi infection of Litopenaeus vannamei Rudi, Mad; Sukenda, Sukenda; Pasaribu, Wesly; Hidayatullah, Dendi
Jurnal Akuakultur Indonesia Vol 18, No 2 (2019): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.18.2.11-20

Abstract

ABSTRACTThe objectives of this study were to investigate the antibacterial activity of G. verrucosa extract in test inhibitory zone with different concentrations (500, 1000, 1500, and 2000 mg/L) and  to examine G. verrucosa extract with different dosage (0.5, 1.0, 1.5, 2.0 g/kg) in feed on immune responses (total hemocytes count, phagocytic activity, phenoloxidase activity, respiratory burst) and survival rate in the Litopenaeus vannamei against the pathogenic Vibrio harveyi. Pacific white shrimp with an initial body weight of 5.25±0.55 g was reared in the aquarium (60×30×30 cm3) with a density of 10 shrimp/aquarium. Pacific white shrimp had been fed three times a day as much as 3% in at satiation for 14 days after challenged with V. harveyi. The first results of the inhibitory test showed that all the concentration of G. verrucosa extract was able to inhibit the growth of V. harveyi and the second result showed that the extract of G. verrucosa can increase the immune responses of shrimp. In the result of survival showed that shrimp fed with 0.5, 1.0, 1.5, and 2.0 g/kg has 80, 73, 70, and 70%, respectively. In conclusion, the seaweed extract of G. verrucosa has antibacterial activity and can induce the immune responses and resistance of Pacific white shrimp against V. harveyi infection.Keywords: Gracilaria verrucosa, seaweed, Vibrio harveyi, vibriosis,  Litopenaeus vannamei ABSTRAKTujuan dari penelitian ini adalah untuk menguji aktivitas antibakteri ekstrak G. verrucosa dalam uji zona hambat dengan konsentrasi yang berbeda (yaitu 500, 1000, 1500, dan 2000 mg/L) dan studi perlakuan pengobatan untuk menguji ekstrak G. verrucosa pada pakan dengan dosis yang berbeda (yaitu 0,5; 1,0; 1,5; dan 2,0 g/kg) pada respons imun (yaitu jumlah total hemosit, aktivitas fagositik, aktivitas fenoloksidase, respiratory burst) dan tingkat kelangsungan hidup pada udang vaname terhadap bakteri patogen Vibrio harveyi. Udang vaname dengan berat badan awal 5,25 ± 0,55 g dipelihara di akuarium (60 × 30 × 30 cm3) dengan kepadatan 10 udang/akuarium. Udang vaname  pasifik diberi makan tiga kali sehari 3% at satiation selama 14 hari setelah di uji tantang V. harveyi. Hasil pertama dari uji zona hambat menunjukkan bahwa semua konsentrasi ekstrak G. verrucosa mampu menghambat pertumbuhan V. harveyi dan hasil kedua menunjukkan bahwa pemberian ektrak G. verrucosa dapat meningkatkan respon imun udang. Hasil tingkat kelangsungan hidup menunjukkan bahwa perlakuan pakan udang dengan dosis 0,5; 1,0; 1,5; dan 2,0 g/kg memiliki tingkat kelangsungan hidup masing-masing 80, 73, 70, dan 70%. Kesimpulannya, ekstrak rumput laut G. verrucosa memiliki aktivitas antibakteri dan dapat menginduksi respons imun & ketahanan udang terhadap infeksi V. harveyi.Kata kunci: Gracilaria verrucosa, rumput laut, Vibrio harveyi, vibriosis, udang vaname