Sayu Putu Yuni Paryati
Medical Faculty, Jenderal Achmad Yani University

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Antibodies titer of dogs immunized by anti-idiotypic vaccine detected by using enzyme linked immunosorbent assay Paryati, Sayu Putu Yuni; Soejoedono, Retno D.; Nawangsih, Eka Noneng
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Rabies control programs, including extensive vaccination with attenuated or inactivated vaccines. However, such vaccines are not without problems and can have detrimental effects. Indeed attenuated vaccines can revert to a more virulent form, and inactivated vaccines may produce serious side effects. These facts, have led to the creation of a new generation of vaccines: recombinant-DNA vaccines, synthetic peptide vaccines, and anti-idiotypic vaccines. The aim of this study is to study the result of anti-idiotypic immunization methods in dogs detected by using enzyme linked immunosorbent assay (ELISA). Anti-idiotype antibodies against rabies (Ab2) were isolated from chicken blood, separated by means of ammonium sulfate precipitation, then dialyzed using PBS pH 8.0 for 24 hours at 2 – 8 oC and purified using affinity chromatography column. Three groups of dogs were immunized, group I was immunized intramuscularly (i.m) with purified IgY, group II was immunizded oraly (p.o) with purified IgY and group III was immunized intramuscularly (i.m) with rabies viral vaccines.  The antibody response (Ab3) was detected using Agar Gel Precipitation Test (AGPT). The efficacy of Ab3 was detected using ELISA. By ELISA, the result of immunization indicated that the level of Ab3 titers of anti-idiotypic vaccine immunized dogs intramuscularly are more than 0.5 IU/ml (protective according to WHO standard), and significantly higher than oraly immunization, but it significantly lower than Ab3 titers of rabies viral vaccine immunized dogs. The conclusion of this study is intramuscularly immunization of anti-idiotypic antibodies can induce protective immune response against rabies virus, although its lower than antibodies titer of viral vaccine, it has a good prospect for vaccine development in controlling rabies
Effect of School Community Empowerment Model towards Handwashing Implementation among Elementary School Students in Dayeuhkolot Subdistrict Solehati, Tetti; Kosasih, Cecep Eli; Susilawati, Sri; Lukman, Mamat; Paryati, Sayu Putu Yuni
Jurnal Kesehatan Masyarakat Nasional Volume 11, Issue 3, February 2017
Publisher : Faculty of Public Health Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (261.725 KB) | DOI: 10.21109/kesmas.v11i3.1171

Abstract

Handwashing with soap behavior in Indonesia remains a problem. The cause is associated with lack of awareness in handwashing with soap. This study aimed to determine effect of school community empowerment on handwashing implementation among elementary school students in Dayeuhkolot Subdistrict, Bandung District. This study used quasi experimental design with pretest and posttest, also descriptive and inferential analysis. Samples consisted of 24 teachers, 377 students at 4th – 6th grade and 24 little doctors. The approach method in this study was using integrated school health effort (combined model of fit for school and selected school health effort) consisting of six stages. Instruments were knowledge questionnaires, observation and checklist sheets. Handwashing with soap was evaluated for three months. Results found that score of little doctors in good category increased in skill of handwashing with soap from 0% to 100%, the skill among the students improved in good category from 0% to 87.5%. School community empowerment affects on handwashing behavior among elementary school students.AbstrakPerilaku mencuci tangan dengan sabun di Indonesia masih menjadi masalah. Penyebabnya dikaitkan dengan kurangnya kesadaran dalam mencuci tangan pakai sabun. Penelitian ini bertujuan untuk mengetahui pengaruh pemberdayaan komunitas sekolah terhadap penerapan mencuci tangan di kalangan siswa sekolah dasar di Kecamatan Dayeuhkolot, Kabupaten Bandung. Penelitian ini menggunakan desain quasi eksperimental dengan pretest dan posttest serta melakukan analisis deskriptif dan inferensial. Sampel terdiri dari 24 guru, 377 siswa di kelas 4-6, dan 24 dokter kecil. Metode pendekatan dalam penelitian ini menggunakan usaha kesehatan sekolah terpadu (gabungan model fit for school dan UKS terpilih), yang terdiri dari enam tahap. Instrumen terdiri dari kuesioner pengetahuan, lembar observasi, dan lembar checklist. Cuci tangan pakai sabun dievaluasi selama tiga bulan. Hasil menemukan bahwa terdapat skor meningkat dalam kategori baik untuk keterampilan cuci tangan pakai sabun guru dari 12,5% menjadi 100%, skor dokter kecil dalam kategori baik meningkat pada keterampilan cuci tangan pakai sabun dari 0% sampai 100%, keterampilan dari cuci tangan pakai sabun pada siswa meningkat dalam kategori baik dari 0% menjadi 87,5%. Pemberdayaan komunitas sekolah memengaruhi perilaku mencuci tangan di kalangan siswa SD.
PCS-6 Preparation and Application of Nano Chitosan Particles as Adjuvan in Rabies Vaccination Based on Anti-Idiotype Antibody Paryati, Sayu Putu Yuni; Hasan, K; Ramadhanti, S
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Rabies is a fatal disease to humans and animals, but can be controlled by prophylaxis administration before being exposed. One type of vaccine that can be utilized is an anti-idiotype antibody vaccine originating from IgY purification of chicken egg yolk. The use of vaccine additives in the form of adjuvants is very important to improve the effectiveness of vaccines [1].Chitosan is non-toxic, easily synthesized, safe to use and able to induce an immune response by activating macrophages. Chitosan can modulate dendritic cell maturation so that it can induce interferon interactions and stimulate activity of T lymphocytes and B lymphocytes. Chitosan can be made in the form of chitosan nanoparticles that can be used to deliver drugs and vaccines through inhalation pathways, orally, intravenously and as non-viral gene delivery vectors. In the oral delivery of chitosan nanoparticles are able to overcome the problem of solubility, protect the drug from enzymatic degradation, controlled release, and extend the time of action in the bloodstream through ionic mechanisms with mucin. Inhalation of chitosan 0.5mg/ml with influenza vaccine produces a good response [2-4].In this work, the preparation of chitosan nanostructures and their application as adjuvan in antibody anti-idiotype rabies vaccination were reviewed.
VPH-4 The Influence of Medium pH on Lactobacillus acidophillus Viability in Soyghurt Tested In Vitro and In Vivo Halimah, Lia Siti; Paryati, Sayu Putu Yuni; Nawangsih, Eka Noneng
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

L. acidophillus bacteria have a high tolerance for acidic atmosphere. This bacterial resistance occurs because the ability to maintain cytoplasmic pH is more alkaline than extracellular pH so that all the different proteins and enzymes in it can still work optimally. L. acidophillus bacteria have cell membranes that are more resistant to cell leakage. Cellular membranes consisting of two layers of phospholipid (lipid bilayer) which on each surface of the layer are attached to proteins and lipid bilayer glycoproteins are semipermeable, which will limit the movement of compounds in and out between the cytoplasm and the external environment. The digestive tract has a different pH, starting from the oral cavity with a pH ranging from 6-7 (interval), stomach with pH 1.5-2 (acid), and intestine with a pH of 8-8.9 (base). Many bacteria can live and have habitat in the oral and intestinal cavities, but only certain bacteria are known to survive in the stomach. The nature of bacteria, in general, does not stand in an acidic atmosphere in the stomach, but there are also pathogenic bacteria (detrimental) that are resistant to the acidic atmosphere in the stomach so that it can cause disease in the digestive system. The stomach is an organ of the digestive system which functions to digest food and as the body's defense organ. The body's defense mechanism is carried out by the stomach against bacteria, namely by removing stomach acid. The presence and endurance of L. acidophillus bacteria in the stomach have not been reported. Therefore, in this study will be tested the effect of soyghurt containing L. acidophillus bacteria on the viability of L. acidophillus bacteria in the stomach and its effect on the gastric fluid profile of male Wistar rats.