Ida Parwati
Departemen Patologi Klinik Fakultas Kedokteran Universitas Padjadjaran/Rumah Sakit Dr. Hasan Sadikin Bandung.

Published : 60 Documents
Articles

PRELIMINARY MAPPING STUDY OF Mycobacterium tuberculosis COMPLEX DNA BY Spoligotyping ON ISOLATED SPUTUM OF TUBERCULOSIS PATIENT FROM 10 PROVINCE CAPITAL CITY Lisdawati, Vivi; Parwati, Ida; Sudarmono, Pratiwi; Sudiro, T. Mirawati; Ramadhany, Ririn; Puspandari, Nelly; Rif’ati, Lutfah; ., Triyani S.
Bulletin of Health Studies Vol. 38 No. 4 December 2010
Publisher : Bulletin of Health Studies

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Mapping TB genotype of Mycobacterium tuberculosis (Mtb) is an importantstudy to identify their distribution or characteristic and also may lead to improve controlof the disease. This study conducted a preliminary mapping of the tubercle bacilli whichhad been circulating in Indonesia. Cultures of DNA isolated from TB patients at urbanareas in 16 provinces in Indonesia, are chosen based on TB Case Detection Rate (CDR)2006 from Indonesia Directorate General of Communicable Disease Control andEnvironment Health (Ministry of Health), were analyzed by spoligotyping for straindifferentiation. In this first part, the analyzed result only came from urban areas in 10provinces, i.e. Palembang, Bandar Lampung, Serang, Jakarta, Bandung, Surabaya,Banjarmasin, Makassar, Pontianak and Ambon.Sample were 269 DNA from 294 isolates collected from sputum of suspect TB patientswith sputum-smear positive (SS+) and age above 15 years old. All samples were obtainedfrom peripheral health laboratory in each province. The procedure collection isaccordance to Indonesia DOTs guidelines (AFB smears) and samples were transportedfrom those 10 areas to Bacteriology Laboratory at CBPRD. Sputum was taken for culturein liquid media MGIT Bactec 960 and solid media Lowenstein Jensen. The DNAs frompositive liquid media MGIT Bactec 960 were isolated and analyzed by spoligotyping toidentify the spoligo pattern. The spoligotyping results converted into octal format withinWords & Excel spreadsheets and compared to International Spoligotype-database(SpolDB4).The previous study (Parwati et.al.) found some differences geographical distributionbetween Beijing genotype strain of tubercle bacilli in West Indonesia compare to EastIndonesia, and the same pattern was also found in this study. Furthermore, the results inthis study showed the differences in spoligo pattern of Mtb complex at 10 urban areas inWest, Middle and East Indonesia. The percentage of Beijing strain family in the samplesfrom West Indonesia showed around 26.61% (31.48% in Sumatra, 28.83% in Java and16.98% in Kalimantan); from the Middle Indonesia around 25.93%; and none werefound in samples from the East Indonesia. The SpolDB4 pattern also showed that themajority of isolates belonged to major clades of M.tuberculosis, i.e. the East African-Indian (EAI); Haarlem (H), Latin American-Mediterranean (LAM), the Central andMiddle Eastern Asian (CAS); U = undefined; T = T family; and the MANU/ Manu family.We also found some isolates of Mycobacterium bovis. There were no significantassociation showed between genotype families and gender, but strong significantassociation found with ethnics and geography. Further confirmation of the results is stillBul. Penelit. Kesehat, Vol. 38, No. 4, 2010: 169 - 185170ongoing (κ value; RFLP and MIRU-VNTR). As conclusion, this study constituted a firstattempt to describe the preliminary mapping of genetic population structure of thetubercle bacilli circulating in Indonesia.Key words: preliminary mapping, Mtb complex, spoligotyping, Beijing genotype strain,SpolDB4
Comparison of Diagnostic Value of Platelet, Leucocyte, NS1 Antigen, and Antidengue IgM Antibody Suwandono, Agus; Nurhayati, Nurhayati; Parwati, Ida; Rudiman, Panji Irani Fianza; Wisaksana, Rudi; Kosasih, Herman; Alisjahbana, Bachti
Journal of the Indonesian Medical Association Vol. 61 No. 8 August 2011
Publisher : Journal of the Indonesian Medical Association

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There are various laboratory tests used to diagnose dengue viral infections. However, not all diagnostic laboratories have those capabilities. Routine hematology tests such as platelet and leukocyte counts are still used by clinicians as supportive tests to diagnose dengue infections because they are available in most primary health centers or small laboratories. This study evaluated the diagnostic use of platelet and leukocyte counts, which are easier and cheaper than dengue NS1 antigen and IgM antibodies, and the appropriate laboratory tests in regard to day(s) of fever. This study used acute specimens from all dengue and non-dengue cases that had been confirmed by a series of dengue diagnostic tests. All the specimens were collected from several studies conducted by US-NAMRU-2, Dr. Hasan Sadikin Hospital/Medical Faculty, Padjadjaran University, and National Institute of Health Research and Development (NIHRD) from 2000 to 2009. This study revealed either thrombocytopenia or leucopenia is an accurate parameter to determine dengue infections starting from day 4 of illness. NS1 test was helpful for diagnosis especially in first and second day of fever whereas IgM antibody is recommended to be used starting from day 5 of fever. J Indon Med Assoc. 2011;61:326-32.Keywords: dengue, thrombocytopenia, leucopenia, NS1 antigen, dengue IgM antibody
Uji Validitas Neutrophil Gelatinase Associated Lipocalin sebagai Penanda Diagnosis Gangguan Ginjal Akut pada Sepsis Hidayat, -; Parwati, Ida; Gondodiputro, Rubin Surachno; Rita, Coriejati
Majalah Kedokteran Bandung Vol 44, No 2 (2012)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Gangguan ginjal akut (GgGA) merupakan penurunan fungsi ginjal secara mendadak yang ditandai dengan peningkatan kreatinin serum ≥0,3 mg/dL atau meningkat >1,5 kali dari kadar sebelumnya atau penurunan urine output (UO) <0,5 mL per jam selama >6 jam. Sepsis merupakan penyebab tersering GgGA dengan angka kejadian berkisar 20–50% dan angka kematian mendekati 70%. Kadar neutrophil gelatinase associated lipocalin (NGAL) urine penderita GgGA dapat meningkat secara cepat dan lebih awal dibandingkan dengan kadar kreatinin serum sehingga NGAL dapat dijadikan penanda diagnosis GgGA. Penelitian bertujuan mengetahui validitas NGAL urine sebagai penanda diagnosis GgGA pada penderita sepsis. Sebanyak 50 sampel urine diambil dari penderita sepsis di Unit Gawat Darurat (UGD), Intensive Care Unit (ICU), dan Medical Intermediate Care (MIC) di Rumah Sakit Dr. Hasan Sadikin Bandung selama Februari sampai Mei 2010 dan dilakukan pemeriksaan kadar NGAL urine dengan metode enzyme linked immunosorbent assay (ELISA). Data yang diperoleh dianalisis dengan uji nonparametrik Mann-Whitney, kurva receiver operating characteristic (ROC), dan uji validitas. Hasil penelitian didapatkan kadar NGAL urine penderita sepsis dengan GgGA lebih tinggi secara bermakna dibandingkan dengan penderita sepsis tanpa GgGA (3.380 ng/mL berbanding 116 ng/mL; p<0,001). Pada cut-off point 107 ng/mL, NGAL urine memiliki sensitivitas 100%, spesifisitas 36%, positive predictive value (PPV) 60,9%, negative predictive value (NPV) 100%, dan akurasi 68%. Simpulan, kadar NGAL urine memiliki validitas yang baik dan dapat dijadikan sebagai penanda diagnosis terjadinya GgGA pada penderita sepsis. [MKB. 2012;44(2):121–6]. Validity Test of Neutrophil Gelatinase Associated Lipocalin as Diagnostic Marker forAcute Kidney Injury on SepsisAcute kidney injury (AKI) is an abrupt decrease of renal function which marked by increase of serum creatinine ≥0.3 mg/dL or ≥1.5 times of previous level or decrease urine output <0.5 mL/hour in >6 hours. Sepsis is the most common cause of AKI with incidence rate is about 20–50% and mortality nearly 70%. Urine neutrophil gelatinaseassociated lipocalin (NGAL) level in AKI patients can increase quickly and earlier compared with serum creatinine and could be as a marker for AKI. The purpose of this study was to assess the validity of urine NGAL as diagnostic marker of AKI on sepsis patients. Subjects were 50 urine samples of sepsis patients from Emergency Department (ED), Intensive Care Unit (ICU) and Medical Intermediate Care (MIC) in Dr. Hasan Sadikin Hospital Bandung between February and May 2010 and were examined with enzyme linked immunosorbent assay (ELISA) method. Data analysis was performed by non parametric Mann-Whitney test, receiver operating characteristic (ROC) analysis and validity test.The results found that urine NGAL of AKI patients were significantly higher compared with non AKI patients (3,380 ng/mL vs 116 ng/mL, p<0.001). A cut-off point >107 ng/mL for urine NGAL had a sensitivity of 100%, specificity of 36%, positive predictive value (PPV) of 60.9%, negative predictive value (NPV) of 100% and accuracy of 68%. In conclusions, urinary NGAL level has good validity and could be used as a screening test for AKI on sepsis patients. [MKB. 2012;44(2):121–6]. DOI: http://dx.doi.org/10.15395/mkb.v44n2.131
Kesesuaian antara Metode Microscopic Observation Drug Susceptibility Assay dan Ogawa pada Biakan Mycobacterium tuberculosis Dewi B., Ni Sayu; Parwati, Ida; Alisjahbana, Bachti; Turbawaty, Dewi Kartika
Majalah Kedokteran Bandung Vol 43, No 2 (2011)
Publisher : Fakultas Kedokteran, Universitas Padjadjaran

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Tuberkulosis (TB) merupakan masalah kesehatan masyarakat di berbagai negara di dunia. Diagnosis pasti TB ditegakkan berdasarkan penemuan kuman M. tuberculosis pada pemeriksaan mikroskopik atau biakan sputum. Biakan merupakan baku emas, namun metode yang digunakan saat ini membutuhkan waktu minimal 8 minggu. Microscopic observation drug susceptibility assay (MODS) merupakan metode biakan untuk M. tuberculosis menggunakan media cair yang dapat sekaligus menguji kepekaan obat TB secara mikroskopik. Tujuan penelitian ini adalah untuk mengetahui kesesuaian metode MODS dengan metode Ogawa (media padat) untuk biakan M. tuberculosis pada penderita TB paru. Penelitian cross sectional telah dilakukan di Departemen Patologi Klinik RSUP Dr. Hasan Sadikin Bandung dan pemeriksaan spesimen dilakukan di Balai Pengembangan Laboratorium Kesehatan (BPLK) Provinsi Jawa Barat periode April–Agustus 2010. Subjek penelitian sebanyak 133 penderita yang didiagnosis tersangka TB paru. Setiap spesimen ditanam pada dua media, media cair MODS dan media padat Ogawa. Analisis statistik kesesuaian metode MODS dengan Ogawa menggunakan uji koefisien Kappa. Terdapat 172 spesimen dari 133 subjek. Kesesuaian antara hasil biakan M. tuberculosis metode MODS dan Ogawa didapatkan nilai Kappa 0,91 yang berarti terdapat kesesuaian yang tinggi antara metode MODS dan Ogawa. Perbandingan waktu pertumbuhan M. tuberculosis secara bermakna lebih cepat (p = 0,000) pada metode MODS, yaitu 10,1 hari (rentang 4–21 hari), dibandingkan dengan metode Ogawa, yaitu 24,8 hari (rentang 14–35 hari). Simpulan, metode MODS dan Ogawa mempunyai angka keberhasilan diagnostik yang relatif sama, keunggulan metode MODS adalah pertumbuhan M. tuberculosis lebih cepat dibandingkan dengan metode Ogawa. [MKB. 2011;43(2):83–8].Kata kunci: Microscopic observation drug susceptibility assay (MODS), M. tuberculosis, Ogawa, sputumConformity Method Between Microscopic Observation Drug Susceptibility Assay and Ogawa Mycobacterium CultureTuberculosis (TB) is a problem of public health that causing high morbidity and mortality rates in various countries in the world. The diagnosis of pulmonary tuberculosis in adults can be established based on the discovery of M. tuberculosis on smear or culture of sputum. Culture is the gold standard but the availlable method is time consuming, it is need minimal eight weeks. Microscopic observation drug susceptibility assay (MODS) is one of methods for M. tuberculosis culture using liquid medium that can be a simultaneously test for M. tuberculosis drug sensitivity. The purpose of this study was to determine the conformity of the MODS method compared with Ogawa method for cultivation of M. tuberculosis in pulmonary TB patients. The cross sectional research has been conducted at Clinical Pathology Department of Dr. Hasan Sadikin Hospital, Bandung and examination of the specimen done at Health Laboratory Development Unit (BPLK), West Java Province between April to August 2010. The subjects were patients who diagnosed as pulmonary TB suspect. Each collected specimen was cultured in liquid media MODS and solid media Ogawa. To analyze the conformity of MODS and Ogawa method, Kappa coefficient of agreement was used. There were 172 specimens collected from 133 subjects. The conformity between culture results of M. tuberculosis in MODS method and in Ogawa’s method using Kappa coefficient, was high (Kappa index 0.91). The difference of growth time of M. tuberculosis significant (p=0.000), in MODS was 10.1 days (range 4–21 days) and in Ogawa method was 24.8 days (range 14–35 days). Conclusion, MODS and Ogawa’s method have the relatively similar diagnostic success rate, the advantage of MODS method is, the growth of M. tuberculosis is faster than in Ogawa method. [MKB. 2011;43(2):83–8].Key words: Microscopic observation drug susceptibility assay (MODS), M. tuberculosis, Ogawa, sputum
Gambaran Foto Toraks Tuberkulosis Paru Genotipe Beijing dan Non-Beijing Soetikno, Ristaniah D.; Parwati, Ida
Majalah Kedokteran Bandung Vol 42, No 3 (2010)
Publisher : Fakultas Kedokteran, Universitas Padjadjaran

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Mycobacterium tuberculosis genotipe Beijing mempunyai virulensi lebih tinggi, tersebar global, dan lebih resisten dibanding non-Beijing. Penelitian analitik observasional dengan rancangan potong silang untuk mengetahui gambaran foto toraks pada kedua genotipe dilakukan periode September 2003–Desember 2005 di Rumah Sakit Hasan Sadikin Bandung. Pembacaan foto toraks oleh dua ahli radiologi. Subjek terdiri dari 206 laki-laki dan 196 perempuan, 131 genotipe Beijing dan 271 non-Beijing. Usia rata-rata 31 tahun. Hasil pembacaan oleh pengamat I pada genotipe Beijing menunjukkan gambaran ringan 60 (45,8%) dan berat 71 (54,2%); genotipe non-Beijing gambaran ringan 205 (75,6%) dan berat 66 (24,4%). Pengamat II untuk genotipe Beijing 62 (47,3%) dan 69 (52,7%);genotipe non-Beijing 208 (76,8%) dan 63 (23,2%). Diperoleh perbedaan bermakna antara genotipe Beijing dan non-Beijing (p<0,05). Kavitasi genotipe Beijing 69 (52,7%) baik pengamat I maupun II, sedangkan non-Beijing berturut-turut 61 (22,5%) dan 60 (22,1%), dengan p<0,05. Kavitas ≥4 cm pada genotipe Beijing 42 (60,0%) oleh pengamat I dan 40 (58,0%) oleh pengamat II, tidak terdapat perbedaan bermakna kavitas <4 dengan ≥4 cm (p>0,05).Kesesuaian foto toraks pengamat I dengan II untuk gambaran ringan 265 dari 270 dan berat 132 dari 137 ( >0,80). Disimpulkan gambaran foto toraks genotipe Beijing lebih berat dan lebih banyak kavitasi. [MKB. 2010;42(3):92-5].Kata kunci: tuberkulosis paru Thoracic Photo Profile of Lung Tuberculosis Beijing and Non Beijing GenotypesBeijing genotype Mycobacterium tuberculosis has higher virulence, spread globally, and more resistant compare with non Beijing. Observational analytic study with cross sectional design to determine the thoracic photos profile of the two genotypes, was done September 2003–December 2005 at Hasan Sadikin Hospital Bandung. Thoracic photos were analyzed by two radiologists. Subjects consisted of 206 men and 196 women comprising of 131 Beijing and 271 non Beijing. The mean age was 31 years. Thoracic photo description by observer I on Beijing showed 60 (45.8%) mild and 71 (54.2%) severe; non Beijing 205 (75.6%) mild and 66 (24.4%) severe. Observer II were 62 (47.3 %) mild and 69 (52.7%) severe of Beijing; 208 (76.8%) and 63 (23.2%) non Beijing, respectively. There was significant difference between Beijing and non Beijing (p <0.05). Cavitation of Beijing by both observer was 69 (52.7%), whereas non Beijing were 61 (22.5%) and 60 (22.1%), respectively (p<0.05). Cavitation of Beijing ≥4 cm was 42 (60.0%) by observer I and 40 (58.0%) by observer II, there was no significant difference between <4 and ≥4 cm (p >0.05). Similarity description from two radiologist was 265 of 270 in mild, and 132 of 137 in severe ( >0.80). In conclusion, thoracic photos of Beijing genotype is significantly more severe with more cavitation. [MKB. 2010;42(3):92-5].Key words: Beijing genotype, lung tuberculosis, non Beijing genotype, thoracic photo
Penentuan Serotipe Virus Dengue dan Gambaran Manifestasi Klinis serta Hematologi Rutin pada Infeksi Virus Dengue Andriyoko, Basti; Parwati, Ida; Tjandrawati, Anna; Lismayanti, Leni
Majalah Kedokteran Bandung Vol 44, No 4 (2012)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Setiap serotipe virus dengue dapat menimbulkan manifestasi klinis mulai dari demam dengue (DD) sampai demam berdarah dengue (DBD) dan sindrom syok dengue (SSD). Sulit untuk menilai karakteristik klinis dan hasil hematologi yang khas untuk setiap serotipe. Tujuan penelitian ini untuk menentukan serotipe virus dengue dan gambaran manifestasi klinis DD, DBD, SSD serta hasil pemeriksaan hematologi rutin yaitu hemoglobin, hematokrit, leukosit, dan trombosit pada setiap serotipe virus dengue. Penelitian dilakukan di Rumah Sakit Dr. Hasan Sadikin Bandung sejak Maret 2010 sampai Juli 2011. Subjek penelitian adalah penderita infeksi dengue usia >14 tahun dengan riwayat demam <5 hari. Sampel darah diambil untuk penentuan serotipe dengue dengan teknik reverse transcription polymerase chain reaction (RT-PCR) dilanjutkan dengan seminested PCR. Data manifestasi klinis dan hematologi rutin diperoleh dari penelusuran rekam medis. Penelitian ini merupakan penelitian deskriptif. Sebanyak 75 penderita ikut dalam penelitian. Serotipe virus dapat dideteksi pada 27 (36%) sampel dengan dominasi DENV-3 (13) diikuti DENV-2 (8), DENV-4 (4), dan DENV-1 (2). Kasus DBD ditemukan paling banyak pada infeksi DENV-3. Infeksi DENV-2 memberikan penurunan hemoglobin tertinggi, persentase peningkatan hematokrit tertinggi, leukosit terendah, dan trombosit terendah dibandingkan dengan serotipe lainnya. Kesimpulan penelitian ini adalah ditemukan keempat serotipe di RS Dr. Hasan Sadikin Bandung yang didominasi serotipe DENV-3. Kasus DBD terutama disebabkan DENV-3. Hasil hematologi rutin paling abnormal ditemukan pada infeksi DENV-2. [MKB. 2012;44(4):253–60]Dengue Virus Serotyping and Its Clinical Manifestation and RoutineHaematology in Dengue InfectionsAll DENV serotypes can cause a spectrum of disease from dengue fever (DF) to dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). It is difficult to differentiate clinical characteristicand hematologic result for each serotype. Aim of this study were to determine dengue serotype and describe clinical manifestation of DF, DHF, DSS and routine hematologic results, i.e.haemoglobin, hematocrit, leukocyte, and thrombocyte in each serotype. This study was conducted at Dr. Hasan Sadikin Hospital Bandung from March 2010 until July 2011. Subjects were dengue patients aged >14 years with a history of fever <5 days. Blood samples were taken for serotype determination by reverse transcription polymerase chain reaction (RT-PCR) followed by semi-nested PCR. Clinical manifestation data and haematologic result were obtained from medical records. This was a descriptive study. Seventy five patients were included in this study. Dengue serotype can be detected in 27 (36%) samples with DENV-3 (13) were dominating followed by DENV-2 (8), DENV-4 (4), and DENV-1 (2). DHF was mainly found in DENV-3. DENV-2 gavethe highest decrease in hemoglobin, highest percentage increase in haematocrit, lowest leukocyte, and lowest thrombocyte. In conclusion, all 4 serotypes are found in RSUP Dr. Hasan Sadikin Hospital Bandung with DENV-3 domination. DHF is mainly caused by DENV-3. DOI: http://dx.doi.org/10.15395/mkb.v44n4.138
Validitas Metode Polymerase Chain Reaction GeneXpert MTB/RIF pada Bahan Pemeriksaan Sputum untuk Mendiagnosis Multidrug Resistant Tuberculosis Sirait, Nurlina; Parwati, Ida; Dewi, Nina Susana; Suraya, Nida
Majalah Kedokteran Bandung Vol 45, No 4 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Pengendalian tuberkulosis saat ini terkendala oleh metode diagnostik konvensional yang lambat terutama untuk mendeteksi multidrug resistant tuberculosis (MDR-TB). Deteksi dini MDR-TB sangat penting untuk mencegah penyebaran MDR-TB dan mengurangi angka kematian. Penelitian ini bertujuan menganalisis validitas pemeriksaan polymerase chain reaction genexpert MTB/RIF suatu pemeriksaan molekuler automatis yang cepat untuk mendeteksi MDR-TB. Penelitian dilakukan di Poliklinik Directly Observed Treatment Short- Course (DOTS) Rumah Sakit Dr. Hasan Sadikin Bandung. Subjek penelitian adalah penderita tersangka MDRTB. Sampel penelitian berupa dahak yang dilakukan pemeriksaan mikroskopis, uji kepekaan metode proporsi media Lowenstein Jensen dan polymerase chain reaction genexpert MTB/RIF. Selama periode Agustus 2012 sampai Januari 2013 didapatkan 88 subjek penelitian, kelompok usia terbanyak 25–34 tahun. Hasil pemeriksaan kultur didapatkan 54 sampel tumbuh, 34 sampel tidak tumbuh, dan 3 sampel merupakan Mycobacteria lain dari tuberkulosis. Sebesar 97,5% sampel yang resisten rifampisin juga resisten terhadap isoniazid. Pemeriksaan GeneXpert MTB/RIF menunjukkan sensitivitas 92,3% dan spesifisitas 75% dengan akurasi 88,2%. Simpulan, pemeriksaan GeneXpert MTB/RIF memiliki validitas tinggi untuk mendiagnosis MDR-TB terhadap baku emas uji kepekaan M. tuberculosis metode proporsi pada media Lowenstein Jensen. Pemeriksaan ini disarankan sebagai alat skrining MDR-TB. [MKB. 2013;45(4):234–9]Kata kunci: GeneXpert MTB/RIF, MDR-TB, uji kepekaan metode proporsiValidity of Polymerase Chain Reaction GeneXpert MTB/RIF Method on Sputum Sample Examination to Diagnose Multidrug Resistant TuberculosisControl of tuberculosis is hampered by slow conventional diagnostic methods especially for the detection of multidrug resistant tuberculosis (MDR-TB). Early detection of MDR-TB is essential to interrupt MDR-TB transmission and reduce the death rate. The aim of this study was to assess the validity of polymerase chain reaction genexpert MTB/RIF examination, which is a rapid automated molecular examination for the detection of MDR-TB. The study was conducted at the Directly Observed Treatment Short-Course (DOTS) clinic at Dr. Hasan Sadikin General Hospital Bandung. Subjects were patients with suspected MDR-TB. The research sample was sputum which was subjected to microscopic examination, susceptibility test proportion methods in Lowenstein Jensen media and polymerase chain reaction genexpert MTB/RIF examination. During August 2012 until January 2013, 88 subjects were obtained, with most of them were 25–34 years old. There were 54 samples obtained that grew in culture and 34 samples did not grow while 3 samples were other Mycobacteria of tuberculosis. It was also found that 97.5% of rifampin-resistant samples were also resistant to isoniazid. Examination of GeneXpert MTB/RIF showed a sensitivity of 92.3%, specificity of 75% with an accuracy of 88.2%. In conclusion, GeneXpert MTB/RIF examination has high validity for diagnosing MDR-TB against M. tuberculosis gold standard resistance test using the proportion method in Lowenstein Jensen media. The examination is recommended for MDR-TB screening. [MKB. 2013;45(4):234–9]Key words: GeneXpert MTB/RIF,MDR-TB, susceptibility test proportion methods DOI: http://dx.doi.org/10.15395/mkb.v45n4.170
Perbedaan Kadar Platelet Activating Factor Plasma antara Penderita Demam Berdarah Dengue dan Demam Dengue Setiabudi, Djatnika; Setiabudiawan, Budi; Parwati, Ida; Garna, Herry
Majalah Kedokteran Bandung Vol 45, No 4 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Manifestasi klinis infeksi virus dengue dapat berupa demam dengue atau keadaan yang lebih berat yaitu demam berdarah dengue. Patogenesis yang menerangkan hal tersebut belum jelas. Teori yang sering dikemukakan yaitu pada penyakit dengue berat terjadi peningkatan kadar mediator proinflamasi. Tujuan penelitian ini untuk melihat perbedaan kadar platelet activating factor plasma penderita demam berdarah dengue dengan demam dengue. Penelitian observasional dengan rancangan potong lintang dilakukan pada Januari–Februari 2013. Subjek penelitian adalah penderita dengue usia 1–14 tahun yang dirawat di Rumah Sakit Dr. Hasan Sadikin Bandung, RSUD Kota Bandung (Ujungberung), dan RSUD Kota Cimahi (Cibabat). Diagnosis dengue dikonfirmasi dengan pemeriksaan antigen nonstruktural-1 dan atau pemeriksaan serologis imunoglobulin M dan G. Sampel darah fase demam, kritis dan pemulihan diambil untuk pemeriksaan kadar platelet activating factor plasma menggunakan metode enzymelinked immunosorbent assay. Selama kurun waktu penelitian didapat 26 penderita dengue, terdiri atas 14 kasus demam dengue dan 12 demam berdarah dengue. Kadar platelet activating factor plasma pada fase kritis penderita demam berdarah dengue [541,45 (239,30–2.449,00)] pg/mL lebih tinggi secara bermakna dibandingkan dengan penderita demam dengue [289,55 (149,50–961,50)] pg/mL; p=0,007. Simpulan, kadar platelet activating factor plasma pada fase kritis penderita demam berdarah dengue lebih tinggi daripada penderita demam dengue. [MKB. 2013;45(4):251–6]Kata kunci: Demam berdarah dengue, demam dengue, platelet activating factor The Difference of Platelet Activating Factor Plasma Level between Dengue Hemorrhagic Fever and Dengue Fever patientsDengue virus infection can manifest as dengue fever and, more severely, as dengue hemorrhagic fever. Their pathogenesis until now is not fully understood. One of the most favorable theories stated the presence of increasing titer of pro-inflammatory mediator in severe dengue. The aim of this study was to determine the difference of plasma platelet activating factor titer between dengue hemorrhagic fever and dengue fever patients. This observational study with cross sectional design was conducted during January–February 2013. Subjects were dengue patients, 1 to 14 years old, hospitalized at Dr. Hasan Sadikin General Hospital, Bandung District Hospital (Ujungberung), and Cimahi District Hospital (Cibabat). Dengue cases were confirmed based on nonstructural-1 antigen and/or immunoglobulin M and G rapid test. Blood samples from febrile, critical and recovery phase were drawn for the examination of platelet activating factor titer using the enzyme-linked immunosorbent assay method. There were 26 dengue cases (14 as dengue fever and 12 as dengue hemorrhagic fever). Plasma platelet activating factor titer at the critical phase was significantly higher in dengue hemorrhagic fever patients [541.45 (239.30–2,449.00)] pg/mL compared to dengue fever patients [289.55 (149.50–961.50)] pg/mL; p=0.007. In conclusion, plasma platelet activating factor titer at the critical phase is higher in dengue hemorrhagic fever patients than in dengue fever patients. [MKB. 2013;45(4):251–6]Key words: Dengue hemorrhagic fever, dengue fever, platelet activating factor DOI: http://dx.doi.org/10.15395/mkb.v45n4.172
Implementation of 25-well culture plates for M. tuberculosis drug susceptibility testing in Indonesia Rosana, Yeva; Sudiro, Tjahjani M.; Alisjahbana, Bachti; Parwati, Ida; van Crevel, Reinout; van Soolingen, Dick
Medical Journal of Indonesia Vol 14, No 3 (2005): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (322.775 KB) | DOI: 10.13181/mji.v14i3.186

Abstract

At present, there is no standardized method for Mycobacterium tuberculosis drug susceptibility testing (DST) among laboratories in Indonesia. Since January 2001 to January 2004 we have tried to establish the method of 25-well culture plates with middlebrook’s media (Drug Susceptibility Culture Plate (DSCP) method) used by the Dutch Supranational Reference Laboratory at the Institute of Public Health and the Environment (RIVM), Bilthoven, Netherlands. Our experience showed that this method potentially gives better result as it can be very well standardized, faster and provides detailed MIC (Minimal Inhibitory Concentration) values. Data from 364 isolates that have been tested by DSCP method showed that resistance to INH, rifampicin, ethambutol, and streptomycin were 21.4%, 19.8%, 15.7%, and 16.5% respectively. Multidrug resistance were found in 13.2% isolates. (Med J Indones 2005; 14: 142-6)Keywords: M. tuberculosis, DST, DSCP method
Efficiency of BacT/Alert in detecting Salmonella Parwati, Ida; Samaun, Endang
Medical Journal of Indonesia Vol 7 (1998): Supplement 1
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (72.439 KB) | DOI: 10.13181/mji.v7iSupp1.1112

Abstract

[no abstract available]
Co-Authors - Hidayat, - - Liza - Noormartany Abdul Hadi Martakusumah Agnes Rengga Indrati Agus Suwandono Ahmad Faried Andi Basuki Prima Birawa Anggraini Alam, Anggraini Ani Melani Maskoen Anna Tjandrawati AR, Ganiem Arto Yuwono Soeroto, Arto Yuwono Bachti Alisjabana, Bachti Bachti Alisjahbana banowati, marni Basti Andriyoko Bethy S. Hernowo Bethy Suryawati Hernowo Budi Setiabudiawan Budiman, Astrid Tamara Maajid Catarina, Erica Chrysanti Murad, Chrysanti Cissy B Kartasasmita Cissy B. Kartasasmita Coriejati Rita Dahlan, Zulkarnain Dewi Kartika Turbawaty Dewi Nurhayati Dick van Soolingen Djatnika Setiabudi DK, Turbawati Effendi, Yusuf Sulaiman Efrida Efrida, Efrida Endang Samaun Endang Sutedja Fairuz Quzwain, Fairuz Fiona Lestari, Fiona Gunawan, J. E. Gurnida, Dida Ahmad Hadyana Sukandar Hartantri, Yovita Hendra Subroto, Hendra Herman Kosasih Herri S. Sastramihardja Herry Garna I, Agnes Rengga Ike Sri Redjeki Indahwaty, - Ivana Agnes Sulianto, Ivana Agnes Johanes C. Mose Johanes Cornelius Mose Juniati, Rianita Larissa, Larissa Lelly Yuniarti, Lelly Leni Lismayanti Livia Noviani, Livia Lutfah Rif’ati Meijerink, Hinta Mike Rezeki Nafsi, Nurizzatun Nafsi, Nurizzatun Nanan Sekarwana Nataprawira, Heda M Nelly Puspandari Nenny Gustiani Ni Sayu Dewi B. Nida Suraya Nina Susana Dewi Nina Tristina Nurhayati Nurhayati Nurlina Sirait Oktavia, D Panji Irani Fianza Rudiman Pratiwi Sudarmono Prayudi Santoso Prihatni, Delita Prihatni, Delita Pujiyanti, Hapsari Rachmat Soelaeman Rachmayati, Silvia Reinout van Crevel Rengga Indrati, Agnes RINI SUNDARI, RINI Ririn Ramadhany Ristaniah D. Soetikno Rita Rachmayanti, Rita Rizal, A Roesli, Rully MA. Rovina Ruslami, Rovina Rubin Surachno Gondodiputro Rudi Wisaksana Sjahid, I. Sjahid, Idaningroem Soeroto, Arto Y Sofiati Dian, Sofiati Sugianli, A K Sugianli, Adhi Kristianto Sumantri, Rahmat Suryana, Nida Sylvia Rachmayati T, Dewi Kartika T. Mirawati Sudiro Tiene Rostini, Tiene Tjahjani M. Sudiro Triyani S. . van der Venn, Andre Vivi Lisdawati Wahyudi, Kumia Wida Purbaningsih, Wida Wulan Ardhana Iswari, Wulan Ardhana Yani Triyani, Yani Yanti Yanti Yeva Rosana Yulius, Elsa Yuwono, Arto