Andi Parenrengi
Balai Penelitian dan Pengembangan Budidaya Air Payau

Published : 44 Documents
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PENAPISAN DAN ANALISIS SPONGE EFEKTIF SEBAGAI ANTIBIOFOULING DI TAMBAK DAN KERAMBA JARING APUNG Suryati, Emma; Parenrengi, Andi; -, Rosmiati; Laining, Asda
MARINA CHIMICA ACTA 1999
Publisher : Universitas Hasanuddin

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Abstract

Crude extracts of sponge has been screened for its antibiofouling properties in fishpond and keramba floating net through several stages such as isolation, identification of attached organisms, bioactivity test of sponge crude extract using asbetic plate, 10 x 5 cm, coated with the extract. Those showing  antibiofouling activity is further tested of its toxicity upon larvae (Balanus amphitrite). The amount of organisms attached on asbetic plate is an activity indicator of sponge extract crude. Field observation was conducted for 40 days with 10 day interval. The result of sponge bioactive screening on attached organisms at floating net provide 8 species among others are; Asterospus sp, Clathria sp, Clathria reinwardi, Desmopasma sp, Dysidea sp,  Haliclona sp, Pericarax sp, dan  Jaspis sp, while in fishpond 4 species i.e;  Clathria sp,  Halichondria sp, Callyspongia sp, and  Jaspis sp  with value of LC50 in 24 consecutive hours as follows:  27,99 ppm; 87,57 ppm ; 112,62 ppm;  dan 117,63 ppm. Extract of  Clathria sp showed highest toxicity and its active fraction is in crude extract while  Halichondria sp is more in its aquoeus fraction. These bioactive components were estimated coming from terpenoid and phenolic acid derivatives.   Key words : Sponge, bioactive, antibiofouling, fishpond, keramba
EKSTRAKSI DNA RUMPUT LAUT Kappaphycus alvarezii DENGAN METODE FENOL KLOROFORM Tenriulo, Andi; Suryati, Emma; Parenrengi, Andi; -, Rosmiat
MARINA CHIMICA ACTA Vol 2
Publisher : Universitas Hasanuddin

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Abstract

Telah dilakukan ekstraksi genom DNA rumput laut Kappaphycus  alvarezii dengan metode konvensional fenol kloroform dengan modifikasi. Sampel yang digunakan berasal dari lokasi budidaya di Pinrang, Madura dan Lombok. Hasil ekstraksi memperlihatkan pita genom DNA yang relatif bersih.  Penambahan kalium asetat dapat meningkatkan kemurnian genom DNA yang diekstraksi. Pengukuran konsentrasi dan kemurnian genom DNA dilakukan dengan menggunakan Spektrofotmeter UV-VIS, diperoleh konsentrasi DNA sekitar 180-240 ng/mL dengan tingkat kemurnian rata-rata 1,89. Hasil elektroforesis menunjukkan bahwa  konsentrasi DNA 1.020 ng cukup untuk memperlihatkan pita yang jelas.   Kata Kunci :  Ekstraksi DNA, Kappaphycus  alvarezii , Fenol kloroform
Pertumbuhan dan Perkembangan Eksplan Rumput Laut Gracilaria verrucosa dan Gracilaria gigas pada Aklimatisasi di Tambak Mulyaningrum, Sri Redjeki Hesti; Parenrengi, Andi; Suryati, Emma
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 20, No 3 (2015): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1750.963 KB) | DOI: 10.14710/ik.ijms.20.3.135-142

Abstract

Aklimatisasi eksplan rumput laut hasil kultur jaringan merupakan proses adaptasi eksplan dengan lingkungan budidaya. Penelitian ini bertujuan untuk mengevaluasi performa pertumbuhan dan perkembangan eksplan rumput laut G. verrucosa dan G. gigas yang diaklimatisasi di tambak dan mendapatkan informasi awal mengenai prospek pengembangan budidaya rumput laut G. gigas di tambak. Eksplan rumput laut G. verrucosa dan G. gigas hasil kultur jaringan dipelihara dalam hapa berukuran 50x50x50 cm dengan berat awal 15 g.hapa-1 dan dipelihara di tambak. Desain penelitian adalah rancangan acak lengkap (RAL) dengan enam unit penelitian terdiri dari tiga ulangan untuk masing-masing spesies. Pemeliharaan eksplan dilakukan selama 60 hari dan setiap 15 hari dilakukan pengukuran bobot, panjang dan perkembangan eksplan serta monitoring terhadap kualitas air. Pengamatan histologi sel rumput laut G. verrucosa dan G. gigas dilakukan dibawah mikroskop. Analisis data pertumbuhan dilakukan dengan uji komparatif independent t-test sedangkan data perkembangan eksplan dan histologi sel rumput laut dianalisis secara deskritif. Pada pemeliharaan di tambak kedua jenis rumput laut memiliki pertumbuhan yang berbeda nyata (P<0,05). Rumput laut G. verrucosa memiliki bobot mutlak lebih tinggi (221,82 g) dari G. gigas (51,94 g) dan LPH (laju pertumbuhan harian) bobot lebih tinggi (3,27%) dari G. gigas (2%). Rumput laut G. verrucosa juga memiliki pertambahan panjang yang lebih tinggi (5,28 cm) dari G. gigas (2,71 cm) dengan LPH panjang masing-masing sebesar 3,06% dan 2,18%. Perkembangan eksplan rumput laut G. verrucosa lebih cepat daripada G. gigas karena faktor fisika dan kimia lingkungan perairan tambak yang tidak sesuai untuk pertumbuhan rumput laut G. gigas yang memiliki susunan sel korteks lebih rapat. Kata kunci: pertumbuhan, perkembangan, G. verrucosa, G. gigas, eksplan, tambak Acclimatization of tissue culture seaweed explants was an adaptation procces of explants to cultivation environment. This study aims to evaluate the growth and development of G. verrucosa dan G. gigas explants on pond acclimatization as early information of pond aquaculture development of G. gigas. Explants of G. verrucosa and G. gigas were rearing on 50x50x50 cm cage net with 15 g.cage-1 of initial weight and cutured on pond. The study was a completely randomized design with six unit experiment including three replicates for each species. Acclimation was done in 60 days then explants weight, length, development, and water quality were monitored every 15 days. G. verrucosa and G. gigas cell histology was observed under microscope. Growth data was analyzed comparatively using independent t-test then explants development and cell histology were represented descriptively. The study showed that the growth of both species was significantly different (P<0.05) on pond cultivation. G. verrucosa had higher weight (221.82 g) than G. gigas (51.94 g) also higher DGR (daily growth rate) (3.27%) than G. gigas (2%). G. verrucosa also had higher elongation (5.28 cm) than G. gigas (2.71 cm) with length DGR of 3.06% and 2.18%, respectively. The development of G. verrucosa explants was better than G. gigas, because of the physical and chemical environment of pond water was not suitable for G. gigas which had dense cortical structure. Keywords: growth, development, G. verrucosa, G. gigas, explants, pond
Binary Vector Construction and Agrobacterium tumefaciens-mediated Transformation of Lysozyme Gene in Seaweed Kappaphycus alvarezii handayani, Tri; Alimuddin, Alimuddin; Widyastuti, Utut; Suryati, Emma; Parenrengi, Andi
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol 21, No 2 (2014)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2014.21.2.348

Abstract

Ice-ice disease is the biggest problem in the cultivation of seaweed Kappaphycus alvarezii. The disease is caused by bacterial infection and induced by drastic changes of water quality. Lysozyme has the ability to break down bacterial cell wall. The purpose of this research was to construct of a binary vector pMSH1-Lys carrying chicken Lysozyme (Lis) gene and introduce pMSH1-Lis on K. alvarezii. The binary vector expression was transformed into Agrobacterium tumefaciens LBA4404 by triparental mating. Thallus was inoculated with A. tumefaciens carrying pMSH1-Lis and then the transformed thallus was selected by adding 20 mg/L hygromycin to the culture medium. PCR analysis showed that the construction of the binary plasmid pMSH1-Lys was established. Percentage of transformation of pMSH1-Lis on K. alvarezii was 23.56%, while the efficiency of regeneration was 11.32%. PCR analysis showed that three of the regenerated thallus contained Lysozyme gene. Thus, transgenic K. alvarezii was produced successfully and this can be useful for studying the mechanisms of seaweed defense against bacterial infection.
Growth, Morphology and Growth Related Hormone Level in Kappaphycus alvarezii Produced by Mass Selection in Gorontalo Waters, Indonesia Fadilah, Siti; Alimuddin, .; Pong-Masak, Petrus Rani; Santoso, Joko; Parenrengi, Andi
HAYATI Journal of Biosciences Vol 23, No 1 (2016): January 2016
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1072.105 KB) | DOI: 10.4308/hjb.23.1.29

Abstract

The use of high quality seed can support the success of the seaweed cultivation. This study was conducted to evaluate the growth performance, morphology and growth related hormone level of brown strain seaweed Kappaphycus alvarezii seed produced by mass selection. Selection was performed in the Tomini Gulf, Gorontalo, based on mass selection of seaweed seed protocol with a slight modification in cut-off 10% of the highest daily growth rate. Selection was carried out for four generations. The selected 4thgeneration of seed was then used in cultivation performance test in the Celebes Sea, North Gorontalo, for three production cycles. The results showed that the selected K. alvarezii has higher clump weight and daily growth rate, longer thallus, more number of branches, and shorter internodes compared to the unselected control and seaweed from the farmer as external control. Furthermore, total sugar content, levels of kinetin hormone and kinetin:indole-3-acetic acid ratio were higher in selected seaweeds than that of unselected control and external control. Thus, mass selection method could be used to produce high growth of seed, and kinetin and indole-3-acetic acid play an important role in growth of K. alvarezii.
Regenerasi Kalus Berfilamen Rumput Laut Kappaphycus alvarezii Pada berbagai Perbandingan Zat Pengatur Tumbuh Auksin (Indole Acetic Acid) dan Sitokinin (Kinetin, Zeatin) Mulyaningrum, Sri Redjeki Hesti; Parenrengi, Andi; Risjani, Yenny; Nursyam, Happy
The Journal of Experimental Life Science Vol 2, No 1 (2012)
Publisher : Graduate School, University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2012.002.01.05

Abstract

Abstrak Interaksi auksin-sitokinin dianggap penting untuk mengatur pertumbuhan dan perkembangan dalam jaringan tanaman. Penelitian ini bertujuan untuk menentukan komposisi auksin dan sitokinin yang tepat untuk regenerasi filamen kalus rumput laut K. alvarezii. Kultur filamen kalus dilakukan pada media cair dengan formulasi ZPT indole acetic acid (IAA) : kinetin : zeatin, dengan komposisi konsentrasi sebagai berikut : A (0,4:0:1) ppm; B (0,4:0,25:0,75) ppm; C (0,4:0,5:0,5) ppm; D (0,4:0,75:0,25) ppm; E (0,4:1:0) ppm; kontrol (tanpa ZPT). Penelitian menggunakan rancangan acak lengkap dengan pengulangan masing-masing perlakuan 3 kali. Parameter yang diamati adalah laju pertumbuhan harian, sintasan, kecepatan regenerasi, panjang tunas dan perkembangan morfologi. Hasil yang diperoleh menunjukkan bahwa formula ZPT terbaik adalah formula A dengan laju pertumbuhan harian 1,929%/hari, sintasan 83,33%, kecepatan regenerasi 41,67% dan rata-rata panjang tunas 44,59 µm. Tunas mulai terbentuk pada 15 hari masa kultur.Kata kunci: formula zat pengatur tumbuh, auksin-sitokinin, K. alvarezii, mikropropagasi
IMPROVEMENT METHOD OF GENE TRANSFER IN Kappaphycus alvarezii Triana, St. Hidayah; Alimuddin, .; Widyastuti, Utut; Suharsono, .; Suryati, Emma; Parenrengi, Andi
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol 8, No 1 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.29244/jitkt.v8i1.13087

Abstract

Method of foreign gene transfer in red seaweed Kappaphycus alvarezii has been reported, however, li-mited number of transgenic F0 (broodstock) was obtained. This study was conducted to improve the method of gene transfer mediated by Agrobacterium tumefaciens in order to obtain high percentage of K. alvarezii transgenic. Superoxide dismutase gene from Melastoma malabatrichum (MmCu/Zn-SOD) was used as model towards increasing adaptability of K. alvarezii to environmental stress. The treat-ments were the culture media and recovery duration, and each treatment consisted of three replica-tions. The best method was co-cultivation using liquid media, then recovery was conducted in liquid media for 10 days. That treatment allowed higher transformation percentage (90%), regeneration effi-ciency (90%), putative bud efficiency (100%), number of buds and explants sprouted (100%) and transgenic explants (100%). The transgenic explants showed an amplification PCR product of Mm-Cu/Zn-SOD gene fragment, whereas the non-transgenic explants showed no amplification product.  All results revealed that suitable method of transgenesis for K. alvarezii has been developed. Keywords:       Agrobacterium tumefaciens, culture media, Kappaphycus alvarezii, recovery duration, transformation
SPONGE MOLECULAR SCREENING FOR ANTIMICROBIAL GENES BY PCR Rosmiati, Rosmiati; Suryati, Emma; Parenrengi, Andi; Sulaeman, Sulaeman
Indonesian Aquaculture Journal Vol 2, No 2 (2007): (December 2007)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (160.132 KB) | DOI: 10.15578/iaj.2.2.2007.127-131

Abstract

Molecular biotechnology approach has been applied on sponge for preventingdiseases on fishery culture. This is important for anticipating and avoiding the using of amount of sponge in nature. The present study aims to screen the antimicrobial (oxytetracycline and chloramphenicol) genes of sponge. DNA extraction of samples was done using the DNeasy Plant mini kit, Phenol-Chloroform and modification of Phenol-Chloroform methods. The presence of oxytetracycline and chloramphenicol genes in sponge was detected using Polymerase chain reaction (PCR) technique. Result of the study showed that four species (Sylotella aurantium, Acanthella kletra, Gelliodes fibulatus and Auletta sp. were amplified for oxytetracycline and two species (Auletta sp. and Pericharax sp.) of sponge were amplified for chloramphenicol at each 226 bp.
GENETIC, COLORATION, AND GROWTH PERFORMANCE OF TWO DIFFERENT VARIETIES OF Kappaphycus alvarezii Sulaeman, Sulaeman; Parenrengi, Andi; Suryati, Emma; Rosmiati, Rosmiati
Indonesian Aquaculture Journal Vol 2, No 1 (2007): (June 2007)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (81.59 KB) | DOI: 10.15578/iaj.2.1.2007.23-26

Abstract

Two different colors (green and brown) of Kappaphycus alvarezii have been farmed in Indonesian waters for many years. This study aimed at comparing two ‘varieties’, i.e. green and brown, both genetically and morphologically. Samples for DNA analysis were collected from a farmer in Pinrang Regency, South Sulawesi. Five universal primers i.e. Ca-01, Ca-02, P-40, P-50, and DALRP were selected to obtain DNA genetic markers in differentiating the green and brown varieties. To compare coloration patterns during cultivation and the growth performance of both varieties, a field experiment was performed in a seaweed farming area in Pinrang Regency, during dry season of August-September 2004. The result of genetic assessment showed that the five selected primers revealed different RAPD banding pattern for both varieties. P-50 and DALRP primers demonstrated the greatest amplification in differentiating RAPD fragment between green and brown varieties. Fragment 900 bp and 1.300 bp were consistently generated in the green variety but were not amplified in the brown variety. The result of the field study confirmed that the coloration pattern of green and brown varieties was fixed; no interchange in color occurred during one crop cultivation.
CHARACTERISTICS OF VIRAL PROTEIN, VP-15, OF WHITESPOT SYNDROME VIRUS ISOLATED FROM INFECTED TIGER SHRIMP Penaeus monodon (Fabricius, 1798) Parenrengi, Andi; Alimuddin, Alimuddin; Tenriulo, Andi
Indonesian Aquaculture Journal Vol 12, No 2 (2017): (December, 2017)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (157.395 KB) | DOI: 10.15578/iaj.12.2.2017.67-75

Abstract

White spot syndrome virus (WSSV) has caused mass mortality on tiger shrimp (Penaeus monodon) culture and adversely affects prawn industry worldwide including Indonesia. It is well known that the protein structure of WSSV plays an important role in the virus infection and morphogenesis process. A viral protein structure called VP-15 is located in the nucleocapsid of virion virus. The protein structure involves in the life cycle of WSSV in host cells. A gene encoding VP-15 could be involved in constructing the RNA interference (RNAi), so it is needed to isolate and characterize for RNAi technology purpose. The study was aimed to isolate and characterize the VP-15 from the infected WSSV tiger shrimp. The characterization of VP-15 was undertaken through assessment of nucleotide sequence, amino acid deduction, alignment nucleotide/protein searches using Genetyx and BLAST program, and dendrogram construction analysis. The results showed that VP-15 was successfully isolated in form of ORFDNA with a fragment size of 243 bp. The phylogenetic tree analysis revealed three clusters corresponding to the time (year) of isolates collection. The VP-15 consisted of 80 amino acids, two start codons (ATG), one stop codon (TAA), and one Kozak context (AAAATGG). Hydrophilic amino acid was the highest composition (44.2%), followed by neutral (31.2%) and hydrophobic (24.6%) amino acid groups. The VP-15 was rich in amino acid of lysine (21.3%), arginine (22.9%) and serine (24.6%). The successful isolation of VP-15 is a very important step in providing a basic yet suitable material in constructing the dsRNA vaccine to control shrimp diseases in aquaculture.