MULYOTO PANGESTU
1. EPRD- Dept. Obstetrics and Gynecology, Monash University, Monash Medical Center,Victoria, Melbourne 2.Lab. Reproductive Physiology, Jenderal Soedirman University, Purwokerto

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Sperm Preservation using Freeze-Drying Method SAILI, TAKDIR; PANGESTU, MULYOTO; SETIADI, MOHAMAD AGUS; AGUNGPRIYONO, SRIHADI; R. TOELIHERE, MOZES; BOEDIONO, ARIEF
HAYATI Journal of Biosciences Vol 12, No 1 (2005): March 2005
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (32.025 KB) | DOI: 10.4308/hjb.12.1.41

Abstract

Since the discovery of cryopreservation method for bull semen, cryopreservation become an alternative method for maintaining gamet resources of certain animal which is threatened or near extinction. This technology was then applied to the preservation of embryo, oocyte, ovary and testis. The application of intracytoplasmic sperm injection (ICSI) for which sperm motility is unnecessary had supported the effort to create simplified method such as freeze-drying for sperm preservation. Due to the benefit of ICSI over the conventional in vitro fertilization (IVF) the spermatozoon could be mechanically driven to pass through the zona pellucida and entering the cytoplasm of oocytes prior to fertilization. The freeze-drying method is an alternative method in sperm preservation which ignored the motility of sperm. The sperm resulted from this technique is in drying state, therefore, it might be stored in room temperature or in refrigerator. Many reports have claimed that freeze-dried sperm which is not motile but has an intact DNA was able to fertilize oocytes, even produced offspring in mouse.
Jumlah Sel Piramidal CA3 Hipokampus Tikus Putih Jantan pada Berbagai Model Stres Kerja Kronik Arjadi, Fitrianto; Soejono, Sri Kadarsih; Maurits, Lientje Setyawati; Pangestu, Mulyoto
Majalah Kedokteran Bandung Vol 46, No 4 (2014)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Paparan stres kronik dan berkepanjangan mengakibatkan hilangnya neuron di regio  CA3 (cornu ammonis) hipokampus dan  penurunan kognitif. Tujuan penelitian  mengetahui perbedaan jumlah sel piramidal CA3 hipokampus tikus putih jantan pada model stres kerja meliputi   paradoxical sleep deprivation (PSD), imobilisasi, dan footshock kronik. Metode penelitian adalah  post-test only with control group design experimental dengan rancangan acak lengkap  menggunakan 24 ekor tikus putih jantan galur Wistar usia 3–4 bulan yang dibagi 4 kelompok:  KI kontrol tanpa perlakuan,  KII (stres PSD), KIII (stres imobilisasi), dan KIV (stres footshock) dan sel piramidal CA3 hipokampus  diwarnai dengan toluidine-blue. Jumlah sel dihitung menggunakan  perangkat lunak Image raster v2.1, perbesaran 400x tiap 10 lapangan pandang. Penelitian dilakukan  6 bulan (April–September 2012) di Laboratorium Hewan Coba, Fakultas Kedokteran dan Ilmu Kesehatan Universitas Jenderal Soedirman. Analisis data menggunakan analysis of variance (ANOVA) dengan Post-Hoc least significant difference (LSD) menunjukkan  perbedaan jumlah sel piramidal CA3 hipokampus signifikan (p=0,037) pada minimal dua kelompok perlakuan. Uji statistik dengan Post-Hoc LSD menunjukkan perbedaan signifikan jumlah sel piramidal CA3 hipokampus antara  kelompok kontrol (12,9±2,47) dan kelompok imobilisasi (9,00±1,53) (p<0,05). Simpulan, kelompok imobilisasi kronik memiliki jumlah sel piramidal CA3 hipokampus terendah dibandingkan dengan ketiga kelompok lainnya.Kata kunci:  Model stres kerja kronik, sel piramidal CA3 hipokampus, tikus putih jantanNumber of CA3 Pyramidal Cell in Male Albino Rat   Hippocampus Exposed to Various Chronic Work Stress Models AbstractProlonged and chronic exposure to stress leads to the loss of neurons at the CA3 (cornu ammonis) hippocampus region and spatial memory deficits. The aim of this study was to study the number of CA3 pyramidal cells in albino rats that were exposed to chronic stress of works model consisting of paradoxical sleep deprivation (PSD), immobilization, and foot shock stresses. The method applied was the post-test only method with control group experimental design using completed randomized design (CRD on 24 3–4 month old male Wistar rats . The rats were divided into 4 groups: group I (control), group II (PSD stress), group III (immobilization stress), and group IV (footshock stress). The CA3 pyramidal cell hippocampus was stained with toluidine-blue. The number of CA3 pyramidal cell of hippocampus was counted using Image raster v2.1 software at 400x magnification in 10 duplicates for each sample. The study was conducted in six months (April–September 2012) at the Animal Laboratory, Faculty of Medical and Health Sciences, Jenderal Soedirman University. Analysis for the differences in the number of CA3 pyramidal cells was conducted using analysis of variance (ANOVA) with Post-Hoc LSD. The results of the ANOVA  showed a p value=0.037, meaning that there was significant difference in at least two groupsof treatment. Further statistical test using Post-Hoc LSD showed a significant difference  between the control group (12.9 ± 2.47) and the chronic immobillization group (9,00 ± 1,53) (p<0.05). In conclusion, the chronic immobillization stress group has the lowest average number of hippocampus CA3 pyramidal cells compared to other groups Key words: CA3 pyramidal cell in hippocampus, chronic works stress model, male albino rats DOI: 10.15395/mkb.v46n4.337