ARTINI PANGASTUTI
Jurusan Biologi,Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas sebelas Maret

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Ripening for improving the quality of inoculated cheese Rhizopus oryzae ANA ESTIKOMAH, SOLIKAH; *, SUTARNO; PANGASTUTI, ARTINI
Nusantara Bioscience Vol 2, No 1 (2010): Nusantara Bioscience
Publisher : “Bioscience Community”, School of Graduates, Sebelas Maret University, Surakarta

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Abstract

Estikomah SA, Sutarno, Pangastuti A 2010. Ripening for improving the quality of inoculated cheese Rhizopus oryzae.Nusantara Bioscience 2: 1-6. Cheese is dairy product resulted from fermented milk in which the fermentation process can be done bylactic acid bacteria or fungus. Rhizopus oryzae is able to produce lactic acid, protease and lipase. The ripening process changes the tasteand texture. The purpose of this study is ripening to improve the quality of inoculated cheese R. oryzae. In this research the ripening wasconducted the concentration variation of temperature (5oC, 10oC, 15oC), and time (7 days, 14 days). The procedure of research consistedof two steps, namely un-ripened cheese preparation followed by ripening cheese preparation. Cheese produced in this study analyzed thevalue of pH, fat content, protein content, amino acid levels and identification of microbe with ANOVA then followed by DMRT at 5%level of significance. Data results were analyzed with the like’s nonparametric statistical test, followed by Fridman Wilcoxon SignedRank Test (WSRT) at 5% level significance. The results showed that the preferred ripened cheese panelist was at a temperature of 15oCfor 14 days. Ripening conditions affect pH, fat content, protein content and do not affect the levels of amino acids that formed ripenedcheese. The best quality ripened cheese i.e. at a temperature of 15°C for 14 days, had a pH value of 4.40, the highest protein content of9.78%, and fat content of 35.02%. The results of identified microbe in un-ripened cheese and ripened cheese include Enterococcus hirae(Enterococcus faecalis), Bacillus subtilis, and Aspergillus sp.
EFFECT OF POVIDONE IODINE TREATMENT ON BACTERIAL COMMUNITY ASSOCIATED WITH WHITE SHRIMP (LITOPENAEUS VANNAMEI) LARVAE Pangastuti, Artini; Suwanto, Antonius; Lestari, Yulin; Suhartono, Maggy T.
Marine Research in Indonesia Vol 34, No 2 (2009)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13039.897 KB) | DOI: 10.14203/mri.v34i2.471

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The efficacy of povidone iodine as a surface disinfectant was assessed in white shrimp (Litopenaeus vannamei) eggs and larvae. Eggs and nauplii were exposed to povidone iodine 20 ppm for 20 seconds. Culture-based method and Terminal Restriction Fragment Length Polymorphism (T-RFLP) were used to monitor the total number of bacteria and diversity of the bacterial community associated with shrimp eggs and larva at each developmental stage. Povidone iodine reduced the total culturable bacteria, especially Vibrio, on eggs and nauplii, as well as the total bacteria in the whole community as estimated by T-RFLP results. Povidone iodine also reduced the diversity of bacterial community and altered the evenness of phylotypes distribution suggesting that the use of povidone iodine as a surface disinfectant in shrimp aquaculture should be reconsidered.
Pemanfaatan potensi Azolla microphylla sebagai pakan untuk ikan sidat (Anguilla bicolor) Kristiawan, Rory Ade; Budiharjo, Agung; Pangastuti, Artini
DEPIK Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Vol 8, No 1 (2019): April 2019
Publisher : Faculty of Marine and Fisheries, Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (439.92 KB) | DOI: 10.13170/depik.8.1.12842

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Abstract. Azolla microphylla is included Azollaceae family that very rich of protein, essential amino acid, vitamin and mineral. Azolla plant can be a potential source of nutrient for fish. The study was aimed to determine the utilization of potential of A. microphylla as a feed for Anguilla bicolor bicolor. This study was used a completely randomized design (CRD), with five treatments and three replications. About 15 of A. bicolor bicolor with an average body length of 20-25 cm and a weight of ± 10 g were divided into five groups, into each aquarium containing three fish and they have been given treatment test by substitution of A. microphylla (0%, 10%, 20%, 30%, 40%) for 30 days. The parameters of this study were absolute weight gain, specific growth rate, feed convertion ratio and survival rate of A. bicolor. The ANOVA analysis were continued by the Tukey test in a confidence level of 95%, has shown difference results significantly (P <0.05) for control treatment (0% A. microphylla) which were absolute weight gain: 0,13±0,07 g, SGR: 0,55±0,11(%/day), FCR: 13,25±2,62 and SR: 100%. All treatment that substituted by A. microphylla haven’t shown positive results. Based results of the research, concentrations of A. microphylla which has been substituted (10%, 20%, 30%, 40%) for 30 days, it hasn’t shown potential feed for A. bicolor bicolorKeywords: Azolla microphylla, Anguilla bicolor bicolor, feed, substitution, potentialAbstrak. Azolla microphylla termasuk dalam famili Azollaceae yang sangat kaya protein, asam amino esensial, vitamin dan mineral. Tanaman azolla dapat menjadi sumber nutrien potensial untuk ikan. Anguilla bicolor bicolor merupakan salah satu ikan yang memiliki potensial ekspor dengan permintaan pasar dunia yang meningkat dari tahun ke tahun.. Tujuan penelitian ini adalah mengetahui pemanfaatan potensi A. microphylla  sebagai pakan untuk ikan sidat A. bicolor bicolor. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL), dengan lima perlakuan dan tiga kali ulangan. Sekitar 15 ekor A. bicolor bicolor dengan rata-rata panjang tubuh 20-25 cm dan berat ± 10 g dibagi menjadi lima kelompok, ke dalam akuarium masing-masing berisi tiga ikan untuk diberi pakan uji dengan substitusi A. microphylla (0%, 10%, 20%, 30%, 40%) selama 30 hari. Parameter penelitian ini adalah pertambahan berat mutlak, laju pertumbuhan spesifik, rasio konversi pakan dan tingkat kelangsungan hidup A. bicolor bicolor. Hasil analisis ANOVA dilanjutkan uji Tukey dengan taraf kepercayaan 95%, menunjukkan hasil beda nyata (P<0,05) pada perlakuan kontrol (0% A. microphylla) dengan pertambahan berat mutlak: 0,13±0,07 g, SGR: 0,55±0,11(%/hari), FCR: 13,25±2,62 dan SR: 100%. Seluruh perlakuan dengan substitusi A. microphylla belum menunjukkan hasil positif untuk ikan sidat. Dari hasil penelitian dapat disimpulkan bahwa pemberian A. microphylla dengan substitusi konsentrasi (10%, 20%, 30%, 40%) selama 30 hari, belum menunjukkan potensi sebagai pakan untuk A. bicolor bicolor.Kata Kunci : Azolla microphylla, Anguilla bicolor bicolor, pakan, substitusi, potensi