ARTINI PANGASTUTI
Jurusan Biologi,Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas sebelas Maret

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Ripening for improving the quality of inoculated cheese Rhizopus oryzae

Nusantara Bioscience Vol 2, No 1 (2010): Nusantara Bioscience
Publisher : “Bioscience Community”, School of Graduates, Sebelas Maret University, Surakarta

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Abstract

Estikomah SA, Sutarno, Pangastuti A 2010. Ripening for improving the quality of inoculated cheese Rhizopus oryzae.Nusantara Bioscience 2: 1-6. Cheese is dairy product resulted from fermented milk in which the fermentation process can be done bylactic acid bacteria or fungus. Rhizopus oryzae is able to produce lactic acid, protease and lipase. The ripening process changes the tasteand texture. The purpose of this study is ripening to improve the quality of inoculated cheese R. oryzae. In this research the ripening wasconducted the concentration variation of temperature (5oC, 10oC, 15oC), and time (7 days, 14 days). The procedure of research consistedof two steps, namely un-ripened cheese preparation followed by ripening cheese preparation. Cheese produced in this study analyzed thevalue of pH, fat content, protein content, amino acid levels and identification of microbe with ANOVA then followed by DMRT at 5%level of significance. Data results were analyzed with the like’s nonparametric statistical test, followed by Fridman Wilcoxon SignedRank Test (WSRT) at 5% level significance. The results showed that the preferred ripened cheese panelist was at a temperature of 15oCfor 14 days. Ripening conditions affect pH, fat content, protein content and do not affect the levels of amino acids that formed ripenedcheese. The best quality ripened cheese i.e. at a temperature of 15°C for 14 days, had a pH value of 4.40, the highest protein content of9.78%, and fat content of 35.02%. The results of identified microbe in un-ripened cheese and ripened cheese include Enterococcus hirae(Enterococcus faecalis), Bacillus subtilis, and Aspergillus sp.

EFFECT OF POVIDONE IODINE TREATMENT ON BACTERIAL COMMUNITY ASSOCIATED WITH WHITE SHRIMP (LITOPENAEUS VANNAMEI) LARVAE

Marine Research in Indonesia Vol 34, No 2 (2009)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

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Abstract

The efficacy of povidone iodine as a surface disinfectant was assessed in white shrimp (Litopenaeus vannamei) eggs and larvae. Eggs and nauplii were exposed to povidone iodine 20 ppm for 20 seconds. Culture-based method and Terminal Restriction Fragment Length Polymorphism (T-RFLP) were used to monitor the total number of bacteria and diversity of the bacterial community associated with shrimp eggs and larva at each developmental stage. Povidone iodine reduced the total culturable bacteria, especially Vibrio, on eggs and nauplii, as well as the total bacteria in the whole community as estimated by T-RFLP results. Povidone iodine also reduced the diversity of bacterial community and altered the evenness of phylotypes distribution suggesting that the use of povidone iodine as a surface disinfectant in shrimp aquaculture should be reconsidered.

Pemanfaatan potensi Azolla microphylla sebagai pakan untuk ikan sidat (Anguilla bicolor)

DEPIK Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Vol 8, No 1 (2019): April 2019
Publisher : Faculty of Marine and Fisheries, Syiah Kuala University

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Abstract

Azolla microphylla termasuk dalam famili Azollaceae yang sangat kaya protein, asam amino esensial, vitamin dan mineral. Tanaman azolla dapat menjadi sumber nutrien potensial untuk ikan. Anguilla bicolor merupakan salah satu ikan yang memiliki potensial ekspor dengan permintaan pasar dunia yang meningkat dari tahun ke tahun.. Tujuan penelitian ini adalah mengetahui pemanfaatan potensi A. microphylla  sebagai pakan untuk ikan sidat (A. bicolor). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL), dengan lima perlakuan dan tiga kali ulangan. Sekitar 15 ekor A. bicolor dengan rata-rata panjang tubuh 20-25 cm dan berat ± 10 g dibagi menjadi lima kelompok, ke dalam akuarium masing-masing berisi tiga ikan untuk diberi pakan uji dengan substitusi A. microphylla (0%, 10%, 20%, 30%, 40 %) selama 30 hari. Parameter penelitian ini adalah pertambahan berat mutlak, laju pertumbuhan spesifik, rasio konversi pakan dan kelangsungan hidup A. bicolor. Hasil analisis ANOVA dilanjutkan uji Tukey dengan taraf kepercayaan 95%, menunjukkan hasil beda nyata (P<0,05) pada perlakuan kontrol (0% A. microphylla) dengan pertambahan berat mutlak: 0,13±0,07g, SGR: 0,55±0,11(%/hari), FCR: 13,25±2,62 dan SR: 100%. Seluruh perlakuan dengan substitusi A. microphylla belum menunjukkan hasil positif untuk ikan sidat. Dari hasil penelitian dapat disimpulkan bahwa pemberian A. microphylla dengan substitusi konsentrasi (10%, 20%, 30%, 40%) selama 30 hari, belum menunjukkan potensi sebagai pakan untuk A. bicolor.