Dimas A. Pamungkas
Jurusan Perikanan Fakultas Pertanian UGM

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PENGEMBANGAN METODE LOOP-MEDIATED ISOTHERMAL AMPLIFICATION OF DNA DAN APLIKASINYA UNTUK DETEKSI KOI HERPES VIRUS PADA BEBERAPA JENIS IKAN Murwantoko, Murwantoko; Triyanto, Triyanto; Pamungkas, Dimas A.
Jurnal Perikanan Universitas Gadjah Mada Vol 12, No 2 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.443 KB) | DOI: 10.22146/jfs.2948

Abstract

The purposes of this experiment were to establish loop-mediated isothermal ampli?cation of DNA ( LAMP) method and its application to observe the presence and duration of koi herpes virus ( KHV) in freshwater ?shes after infection. The infection was carried out using 4-6 cm length of java barb (Barbodes gonionotus), grass carp (Ctenoparingodon idella), gold ?sh (komet) (Carassius auratus), tambaqui (Colossoma macropomum) and koi (Cyprinus carpio koi) ?shes. Fishes were infected intraperitoneally with KHV inoculums and 2 ?shes were sampled everyday. DNA was extracted from gill and used for diagnosis with LAMP assays. The result of this experiment showed LAMP assay can be established and gave 100 times more sensitive than conventional PCR assay. Koi was con?rmed as a host of KHV. Java barb, tambaqui, gold ?sh and grass carp can serve as vector for KHV and the presence of KHV was detected in java barb and tambaqui for 4 days, gold?sh and grass carp for 5 days after infection.