Widiyanti P
Institute of Tropical Disease. Airlangga University. Surabaya, Indonesia, Tropical Infection Diviston, Department of Internal Medicine Dr Soetomo Hospital-Faculty of Medicine Airlangga University, Surabaya, lndonesia

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COLLOID AND METHYLPREDNISOLONE THERAPY AS ALTERNATIVE MANAGEMENT OF DHF Nasronudin, Nasronudin; P, Widiyanti; MV, Arfijanto; M, Rusli
Buletin Penelitian Sistem Kesehatan Vol 13, No 3 Jul (2010)
Publisher : Buletin Penelitian Sistem Kesehatan

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Abstract

There are three phase in progresivity of dengue infection which are afferent phase, efferent phase and efector phase. In dengue infection, it has been found endothelial cells leak age, the pro inflammatory cytokine level alteration and other mediator followed by the plasma migration and it has the potency to become dengue shock. The mam clinical manifestationare fever and bleeding. The aim of this research is to prove the influence of colloid and methylpredmsolone theraphy to inhabit the endothelial cells leakage through the alteration of IL-1b. TNF-a and PLA-2 level. This research has been involved 36 dengue patients in Tropical Infection Division, Departement of lntemal Medicine Medical Faculty Airlangga University -Dr. Soetomo Hospital Surabaya and 36 control group. We did the examination of cytokine level (IL-1b. TNF-a PLA-2) on blood sample with ELISA method. The dose methylprednisolone 125 mg twice a day and colloid (MW 40 kDa) 500 cc perday for 3 days has been given to the patient whose fulfilled the inclusion critena. We found temperature change to normal level after intervention. In conclusion, colloid and methylprednisolone therapy is effective to accelerade the temperature, IL -1b, TNF-a, and PLA-2 level declination, accelerate the endothelial cells sealing and plasma migration in dengue infective patient. Key words: DHF, Colloid, Methylprednisolone, proinflammatory cytokine
Genetic variability of Candida albicans in HIV/AIDS patient with and without ARV therapy and non HIV/AIDS Rahayu, Retno Puji; P, Widiyanti; M, Arfijanto
Dental Journal (Majalah Kedokteran Gigi) Vol 45, No 1 (2012): (March 2012)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v45.i1.p28-34

Abstract

Background: Oral candidiasis is the mostly found oral manifestation in HIV/AIDS infected patient caused by immunocompromised especially immunodeficiency. Clinical symptoms is severe pain in oral cavity and dry mouth because of xerostomia which cause the loss of appetite. Candida albicans (C. albicans) is normal flora in oral cavity which plays as opportunistic pathogen and also the cause of oral candidiasis. Almost 90% of HIV–infected patient have oral candidiasis. This condition is clinical problem which has not been well-managed yet. C. albicans colonized oral mucous cavity has different genetic variability for each strain. Phenotype of C. albicans has been determined by genetic factor and environtment. This condition stimulate differences of genotype among various strain of C. albicans in the world. Purpose: The purpose of this research is to analyze the genetic variability of C.albicans which colonized in the mucous oral cavity of HIV/AIDS patient in Surabaya in the treatment with and without ARV therapy and non HIV/AIDS. Methods: This research has been identify and characterize the prevalent strain of C. albicans isolat in Surabaya (East Java) in HIV/AIDS infected patient with oral candidiasis by method of Iatron candidal check. The highlight of this research including cytology examination by Papanicoloau staining, C. albicans culture, spheroplast making, DNA isolation and genetic variability checking by randomly amplyfied polymorphism DNA (RAPD). Results: C. albicans colonizing oral mucosa of non-HIV patients had a predisposition of farther genetic relationship (genetic distance of 0.452) with C. albicans colonizing oral mucosa of HIV ARV and HIV non-ARV patients. The genetic distance was ranging between 0 and 1, where 9 was long genetic distance and 1 was short genetic distance. In contrast, C. albicans colonizing oral mucosa of HIV ARV have predisposition of closer genetic relationship (genetic distance of 0.762) with C. albicans colonizing oral mucosa of HIV non-ARV patients. Conclusion: The conclusion of this research were C.albicans colonizing HIV/AIDS patiens with and without ARV showed no high genetic variability between C.albicans isolate in HIV patients. There fore, the character of C.albicans colonizing HIV ARV and HIV non-ARV patients had similar genotype predisposition of closer relationship value with C.albicans colonizing oral mucosa non HIV patients.Latar belakang: Oral candidiasis merupakan manifestasi kelainan rongga mulut yang paling sering timbul pada penderita HIV/AIDS karena kondisi immunocompromised terutama defisiensi imun. Gejala klinisnya berupa nyeri hebat di rongga mulut dan mulut kering karena xerostomia yang menyebabkan hilangnya nafsu makan. Candida albicans (C. albicans) berperan sebagai patogen oprtunistik dan merupakan penyebab Kandidiasis rongga mulut. Hampir 90% penderita terinfeksi HIV mengalami kandidiasis rongga mulut. Kondisi ini merupakan masalah klinis yang belum teratasi dengan baik. Kolonisasi C. albicans di mukosa rongga mulut mempunyai variabilitas genetic yang berbeda untuk tiap strainnya. Fenotip C. albicans ditentukan oleh faktor genetic dan lingkungan. Kondisi ini menstimulir perbedaan genotip di antara berbagai strain C. albicans di dunia. Tujuan: Tujuan penelitian ini adalah meneliti korelasi antara hubungan genetik yang menunjukkan variasi genetik kolonisasi C. albicans pada rongga mulut dan insidens kandidiasis rongga mulut pada penderita HIV/AIDS dan non-HIV/AIDS. Metode: Penelitian ini mengidentifikasi dan mengkarakterisasi strain Candida albicans isolat Surabaya (Jawa Timur) pada penderita HIV/AIDS dengan kandidiasis rongga mulut dengan metode iatron candidal check. Penekanan dalam penelitian ini termasuk pada pemeriksaan sitologi dengan pengecatan Papanicoloau, kultur C. albicans, pembuatan spheroplast, isolasi DNA dan pemeriksaan variabilitas genetik dengan randomly amplified polymorphism DNA (RAPD). Hasil: C. albicans yang berkolonisasi di rongga mulut pada penderita non-HIV mempunyai predisposisi hubungan genetik (jarak genetik 0.452) dengan C. albicans yang berkolonisasi di rongga mulut pada penderita HIV yang mendapatkan terapi ARV dan non ARV. Jarak genetic bervariasi antara 0 dan 1, dimana 9 dalah jarak genetik terpanjang and 1 adalah jarak genetik terpendek. Sebaliknya, C. albicans yang berkolonisasi di rongga mulut pada penderita HIV yang menerima terapi ARV memiliki predisposisi hubungan genetic yang lebih dekat (jarak genetic 0.762) dibandingkan C. albicans yang berkolonisasi di rongga mulut pada penderita HIV non-ARV. Kesimpulan: Sebagai kesimpulan, penelitian ini menunjukkan bahwa C. albicans isolate Surabaya yang mengkolonisasi penderita HIV/AIDS dengan ARV dan non ARV memiliki hubungan kekerabatan genetik yang sama dibanding dengan pasien non HIV/AIDS.
In vivo characterization of polymer based dental cements P, Widiyanti; Siswanto, Siswanto
Dental Journal (Majalah Kedokteran Gigi) Vol 44, No 4 (2011): (December 2011)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v44.i4.p173-176

Abstract

Background: In vivo studies investigating the characterization of dental cements have been demonstrated. As few in vitro studies on this cement system have been performed. Previous researches in dental material has been standardized dental cement which fulfilled the physical and mechanical characteristic such as shear strength but were on in vitro condition, the animal model and clinical study of dental cement from laboratory has not been done yet. This research examined physical and mechanical characteristic in vivo using rabbit by making the caries (class III) in anterior teeth especially in mesial or distal incisive, fulfilled the cavity by dental cement and analyzed the compressive strength, tensile strength, and microstructure using scanning electron microscope (SEM). Purpose: This study is aimed to describe the in vivo characterization of dental cements based on polymer (zinc phosphate cement, polycarboxylate, glass ionomer cement and zinc oxide eugenol). Methods: First, preparation was done on animal model’s teeth (6 rabbits, male, 5 months old). The cavity was made which involved the dentin. Then the cavity was filled with dental cement. After the filling procedure, the animal model should be kept until 21 days and than the compressive test, tensile test and microstructure was characterized. Compressive test and tensile test was analyzed using samples from extracted tooth and was measured with autograph. The microstructure test was measured using SEM. Results: The best compressive strength value was belongs to zinc phosphate cement which was 101.888 Mpa and the best tensile strength value was belongs to glass ionomer cement which was 6.555 Mpa. Conclusion: In conclusion, comparing with 3 others type of dental cements which are zinc phosphate, polycarboxylate and glass ionomer cement, zinc oxide eugenol cement has the worst for both physical and mechanical properties.Latar belakang: Studi in vivo meneliti karakterisasi secara in vivo dari semen gigi. Beberapa studi in vitro di bidang ini telah dilakukan. Beberapa riset di bidang material gigi telah menghasilkan semen gigi yang memenuhi standart sifat fisik dan mekanik seperti regangan dan kekuatan secara in vitro, sedangkan uji in vivo dan uji klinis dari semen gigi dari laboratorium belum dilakukan. Penelitian ini menguji karakteristik fisik dan mekanik semen gigi menggunakan hewan coba kelinci dengan membuat karies kelas III di gigi anterior terutama di permukaan mesial atau distal insisif, mengisi kavitas dengan semen gigi dan menganalisa kekuatan tekan, kekuatan tarik dan struktur mikronya dengan menggunakan scanning electron microscope (SEM). Tujuan: Studi ini bertujuan memberikan gambaran karakterisasi in vivo semen gigi berbahan dasar polimer (semen seng fosfat, polikarboksilat, ionomer kaca dan seng oksida eugenol). Metode: Pertama, kami melakukan preparasi pada gigi hewan coba (6 kelinci, jantan, usia 5 bulan). Kemudian kita membuat kavitas yang melibatkan dentin. Lalu kami menumpat kavitas dengan semen gigi. Setelah prosedur penumpatan, hewan coba dipelihara selama 21 hari dan dikarakterisasi kekuatan tekan, kekuatan tarik dan struktur mikronya. Kekuatan tekan dan kekuatan tarik dianalisa dari sampel uji gigi hewan coba yang diekstraksi dan diukur dengan autograf. Struktur mikronya diuji dengan SEM. Hasil: Hasil nilai kuat tekan terbaik diperoleh oleh semen seng fosfat (zinc phosphate cement) sebesar 101,888 Mpa dan nilai kuat tarik semen gigi terbaik adalah semen gelas ionomer (glass ionomer cement) sebesar 6,555 Mpa. Kesimpulan: Dapat disimpulkan, dari ketiga jenis bahan semen yaitu seng fosfat, polikarboksilat, dan ionomer kaca, yang mempunyai sifat fisik dan mekanikal terburuk adalah semen ionomer kaca.