Philips Onggowidjaja
Universitas Kristen Maranatha

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Kehadiran Bakteri Coliform dalam Es Batu pada Tiga Rumah Makan Ayam Goreng Siap Saji di Bandung ., Michael; Onggowidjaja, Philips; Rusmana, Djaja
Jurnal Kedokteran Maranatha Vol 9, No 2 (2010)
Publisher : Universitas Kristen Maranatha

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Some reports have shown that ice cubes may contain Enterobacteriaceae bacteria. This descriptive, observational research aims to analyze the microbiological quality of the ice cubes served at three selected restaurants  (1,2,3).  Three  samples  of  ice  cubes  were  rinsed  with  steril  water,  left  melted,  and  diluted serially.  Each  diluted  sample  was  inoculated  into  MacConkey  Agar  and  EMB  Agar  with  pour  plate method duplo. The data collected were the number of colonies (interpreted as cfu or colony forming unit) of Escherichia coli and other Enterobacteriaceae bacteria. The species of selected colonies were identified by  biochemical reactions. The result showed that sample 1 contained 3 cfu/ml Escherichia coli and 5 cfu/ml Pseudomonas  sp.  Sample  2  contained  10  cfu/ml  Escherichia  coli  and  4  cfu/ml  Pseudomonas  sp.,  but sample 3 didn’t contain Escherichia coli and other Enterobacteriaceae bacteria members. It is concluded that ice cubes from restaurant 3 met the requirement for drinking water, whereas those from restaurants 1 and 2 did not.  Keywords: ice cubes, Escherichia coli, Pseudomonas sp.
Analisis DNA Bacillus sp. BAC4 Hasil Polymerase Chain Reaction (PCR) dengan Primer-Primer yang Dirancang dari Gen pga Onggowidjaja, Philips; Moeis, Maelita Ramdani; Ratnaningsih, Enny
Jurnal Kedokteran Maranatha Vol 8, No 1 (2008)
Publisher : Universitas Kristen Maranatha

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Bacillus sp. BAC4 DNA was amplified using PCR method employing primers designed from pga genes of 5 bacteria (Arthrobacter viscosus, Bacillus megaterium, Escherichia coli, Kluyvera citrophila, and Providentia rettgeri). Analysis  of DNA base direct sequencing of PCR products showed the highest homology of Bacillus sp. BAC4 DNA with Bacillus subtilis DNA at the region from citG to yirG.  This fact states that primers designed from pga genes of 5 bacteria has not been successful in amplifying Bacillus sp. BAC4 pga gene, and that Bacillus sp.  BAC4 pga gene is predicted to be quite different from the existing data of pga genes available at Genbank.  Analysis of DNA base direct sequencing amplified using the pga primers shows the highest homology to the DNA of Bacillus subtilis.
Akurasi Deteksi Mycobacterium tuberculosis dengan Teknik PCR menggunakan “Primer X” dibandingkan dengan Pemeriksaan Mikroskopik (BTA) dan Kultur Sputum Penderita dengan Gejala Tuberkulosis Paru Jasaputra, Diana Krisanti; Onggowidjaja, Philips; Soeng, Sylvia
Jurnal Kedokteran Maranatha Vol 5, No 1 (2005)
Publisher : Universitas Kristen Maranatha

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Akhir-akhir ini, terjadi peningkatan jumlah penderita tuberkulosis. Guna pembe-rantasan penyakit TBC, diagnosis dan deteksi Mycobacterium tuberculosis menjadi amat penting. Deteksi tersebut dapat dilakukan dengan teknik Polymerase Chain Reaction (PCR), pemeriksaan mikroskopik, dan kultur bakteri. Tujuan penelitian ini adalah menilai sensitivitas, spesifisitas, dan akurasi deteksi Mycobacterium tuberculosis dalam sputum penderita TBC paru dengan teknik PCR dibandingkan pemeriksaan secara mikroskopik (Bakteri Tahan Asam/BTA) dan kultur bakteri TBC dalam sputum.Penelitian ini merupakan suatu uji diagnostik yang dirancang secara cross sectional. Penelitian dilakukan terhadap penderita TB paru di BP4 Jl Cibadak Bandung, mulai April 2004 sampai dengan Agustus 2004.Pemeriksaan sputum penderita dilakukan dengan tiga teknik pemeriksaan, yaitu dengan teknik PCR, pemeriksaan BTA secara mikroskopik, dan kultur bakteri.Dibandingkan dengan pemeriksaan Bakteri Tahan Asam secara mikroskospik, deteksi Mycobacterium tuberculosis dengan teknik PCR memiliki sensitivitas 30%, spesifisitas 80%, dan akurasi 47%. Uji kemaknaan dengan Mc Nemar memberikan hasil adanya perbedaan yang bermakna. (p < 0,01).Dibandingkan dengan metode kultur bakteri TBC, deteksi Mycobacterium tuberculosis dengan teknik PCR memiliki sensitivitas 65%, spesifisitas 40%, dan akurasi 57%. Uji kemaknaan dengan Mc Nemar memberikan hasil tidak adanya perbedaan yang bermakna. (p = 1,0). Dibandingkan dengan pemeriksaan Bakteri Tahan Asam secara mikroskospik, deteksi Mycobacterium tuberculosis dengan metode kultur bakteri TBC memiliki sensitivitas 31,6%, spesifisitas 81,8%, dan akurasi 50%. Uji kemaknaan dengan Mc Nemar memberikan hasil adanya perbedaan yang bermakna. (p < 0,01).Dengan demikian, dapat disimpulkan bahwa deteksi Mycobacterium tuberculosis dengan teknik PCR sama baiknya dengan kultur bakteri TBC, namun waktu pemeriksaan dengan teknik PCR lebih singkat dibandingkan dengan kultur bakteri TBC. Mycobacterium tuberculosis banyak tidak terdeteksi dengan pemeriksaan mikroskopik (BTA)
Penurunan Populasi Bakteri dalam Saliva setelah Mengunyah Permen Karet Onggowidjaja, Philips; Tirtanamala, Liessyana; Hendriani, Dian
Jurnal Kedokteran Maranatha Vol 4, No 2 (2005)
Publisher : Universitas Kristen Maranatha

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Research has been performed to know whether chewing gum was effective significantly in decreasing bacteria population in salivla. Saliva was collected from 10 volunteers before chewing gum, 5 minutes after, and 20 minutes after. Saliva samples were diluted then cultured in Nutrient Agar Plates. Grown colonies (cfu, colony forming unit) were counted, data were tabulated and analyzed by using student t test. Chewing gum showed significant effect on decreasing bacteria population in salival, after 5 and 20 minutes of chewing gum.
Keberadaan Salmonella dan Bakteri Coliform pada Bumbu Kacang Baso Tahu ., Indrawaty; Onggowidjaja, Philips
Jurnal Kedokteran Maranatha Vol 2, No 1 (2002)
Publisher : Universitas Kristen Maranatha

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“Baso tahu” is common food.  Salmonella and colifom bacteria commonly contaminate foods.  The presence of both groups was examined from “baso tahu” peanut thick sauce, by growing it on SS agar and MacConkey agar respectively.  Results of biochemical test showed Salmonella did not contaminate it.  The thick sauce was contaminated by 2895,4 coliform bacteria per gr (on average), which exceeded the standard.
Brain Damage due to Excessive Glycine Diet Related to NR2B Protein Onggowidjaja, Philips; Shahib, M. Nurhalim
Jurnal Kedokteran Maranatha Vol 11, No 2 (2012)
Publisher : Universitas Kristen Maranatha

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Damaged brain will lower the quality of life. Several efforts to prevent brain damage have been performed, including the studies to understand the brain damage mechanisms and to find the drugs to prevent further damages. One of the brain damage mechanisms after the trauma caused by ischemia, infection, etc. is the exitotoxic reaction triggered by excessive Ca2+ influx into the neuron. The influx is facilitated by ion channel at neuron, and the performance of this channel is influenced by its subunits, such as NR1, NR2A-D, and NR3A,B. The NR1 subunit helps the opening of the channel after binding Glycine, while the NR2B subunit helps the opening after binding Glutamate. Glycine and Glutamate are ligands working as coagonist to each other. The presence of excessive Glycine is considered to increase the Ca2+ influx, which can lead to neuronal death through apoptotic pathway. Therefore, brain-damage patients’ intake of foodstuff rich in Glycine should be controlled to prevent them from further damage.
THE INFLUENCE OF COMMON CARP MEAT (Cyprinus carpio) DIET ON BRAIN INDEX AND THE EXPRESSION OF GENE NR2B OF SWISS WEBSTER MICE CEREBRUM Onggowidjaja, Philips; Shahib, M. Nurhalim
Journal of Medicine and Health Vol 1, No 2 (2015)
Publisher : Maranatha Christian University

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This research was to evaluate the influence of common carp meat diet on brain index and  gene NR2B expression of  Swiss Webster mice cerebrum.  The gene expression and performance of ion Ca2+ channel in neuron are influenced by Glycine and Glutamic Acid, which present in relatively high quantities in common carp meat.  Eighteen male mice were divided into 3 diet groups, the standard pellet, 120 mg and 480 mg common carp meat/day.  The brain index differences among groups were analyzed using ANOVA test (p≤0,05) and LSD.  The total RNA of 5 mg mouse cerebrum was isolated, followed by RT-PCR; the DNA quantities were predicted using Geldoc instrument, then were analyzed using ANOVA test (p≤0,05).  The 120 mg diet group brain index did not show difference (p=0,284) against the standard diet group, while the 480 mg diet group showed the increase compared to the standard (p=0,034) and 120 mg diet groups (p=0,004).  No DNA quantity differences of all groups (p=0,195).  It was concluded, the 480 mg common carp meat/ day diet increased the brain index of Swiss Webster mice, while the 120 mg and 480 mg common carp/day diets did not influence the gene NR2B expression of Swiss Webster mice cerebrum. Keywords : brain index, gene NR2B expression, common carp meat diet
THE INFLUENCE OF COMMON CARP MEAT (Cyprinus carpio) DIET ON BRAIN INDEX AND THE EXPRESSION OF GENE NR2B OF SWISS WEBSTER MICE CEREBRUM Onggowidjaja, Philips; Shahib, M. Nurhalim
Journal of Medicine & Health Vol 1 No 2 (2015)
Publisher : Universitas Kristen Maranatha

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (404.543 KB) | DOI: 10.28932/jmh.v1i2.505

Abstract

This research was to evaluate the influence of common carp meat diet on brain index and  gene NR2B expression of  Swiss Webster mice cerebrum.  The gene expression and performance of ion Ca2+ channel in neuron are influenced by Glycine and Glutamic Acid, which present in relatively high quantities in common carp meat.  Eighteen male mice were divided into 3 diet groups, the standard pellet, 120 mg and 480 mg common carp meat/day.  The brain index differences among groups were analyzed using ANOVA test (p≤0,05) and LSD.  The total RNA of 5 mg mouse cerebrum was isolated, followed by RT-PCR; the DNA quantities were predicted using Geldoc instrument, then were analyzed using ANOVA test (p≤0,05).  The 120 mg diet group brain index did not show difference (p=0,284) against the standard diet group, while the 480 mg diet group showed the increase compared to the standard (p=0,034) and 120 mg diet groups (p=0,004).  No DNA quantity differences of all groups (p=0,195).  It was concluded, the 480 mg common carp meat/ day diet increased the brain index of Swiss Webster mice, while the 120 mg and 480 mg common carp/day diets did not influence the gene NR2B expression of Swiss Webster mice cerebrum. Keywords : brain index, gene NR2B expression, common carp meat diet