Ida Bagus Made Oka
Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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ISOLASI TOXOPLASMA GONDII PADA AYAM BURAS Apsari, Ida Ayu Pasti; Artama, Wayan Tunas; -, Sumartono; Damriyasa, I Made; Oka, Ida Bagus Made; Swacita, Ida Bagus Ngurah
Buletin Veteriner Udayana Vol. 3 No.2 Agustus 2011
Publisher : Buletin Veteriner Udayana

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A study was conducted Toxoplasma gondii isolate from the brain and the heart of freerangechickens in Bali. The aim of this study to determine the seroprevalence andto isolate Toxoplasma gondii from free-range chicken heart and brain. To achieve of thisstudy observed 311 free-range chicken serum samples with ELISA method and examined225 free-range chicken brain and heart used digestion method. All of the samples takenfrom 9 districts in Bali. The results showed that the seroprevalence of Toxoplasma gondiiinfreerange chicken has 91.64%. Isolation of Toxoplasma godii from the heart and the brainfreerange chicken found the cyst on inoculate heart and brain, but by bioassay in mice for4 weeks observation failed to find tachyzoite form in peritonial exudat
STRATEGI PEMBERANTASAN PENYAKIT CACINGAN PADA ANJING DI SEKITAR OBYEK WISATA SANUR DALAM UPAYA PENCEGAHAN PENULARANNYA YANG BERSIFAT ZOONOSIS APSARI, IDA AYU PASTI; OKA, IDA BAGUS MADE; DAMRIYASA, I MADE; DWINATA, MADE
Jurnal Udayana Mengabdi Volume 5 No.1 – April 2006
Publisher : Jurnal Udayana Mengabdi

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ABSTRACT The role of the dog as a definitive host for a number of zoonotic parasites has been recognized as a significant public health problem especially strey dog population. In this population, poor levels of hygine and overcrowding, together with the lack of veterinary attention and zoonotic awareness, exacerbates the risk of disease infection. A public service was undertaken on Friday 16th September 2005 in Sanur beach to control the dog parasitic zoonoses. About 30 trey dogs and 31 domesticated dogs were treated with anthelmintic to control the helminth infections. All of strey dogs were clinically in poor condition.
Studi Patologi Kejadian Cysticercosis pada Tikus Putih Berata, I Ketut; Arjana, Anak Agung Gde; Sudira, I Wayan; Merdana, I Made; Budiasa, I Ketut; Oka, Ida Bagus Made
Jurnal Veteriner Vol 11, No 4 (2010)
Publisher : Jurnal Veteriner

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Rats are commonly used as animal model in pathological and reproduction research, butunfortunately they are often infected with cysticercosis. The objective of this research was to determinethe pathological changes the of the rats (Rattus novergicus) tissues affected with cysticercus. Thisresearch using 24 of female rats. They were adapted to a new environment for a week and the feeding andwater were provided ad libitum. At the end of adaptation period rats were necropsied and the visceralorgans were examined for pathological changes especially the present of cysticercosis. The liver and kidneyof each rat were soaked in 10% phosphate buffered formalin. Following dehydration process, tissue wereembedded in paraplast, cut at 5 micron and stained with Harris hematoxylin eosin (HE). The resultshowed that 8 of 24 rats were affected by cysticercosis on the liver. The histopathological changes werenecrotic lesions and eosinophylic cells infiltration around the cysticercosis lesion. The results showed that8 of 23 rats were affected by cysticercosis. The presence of necrosis and cells inflammation could interferethe results of the study when such a rats are used. It is therefore necessary to screen rats for cysticercosis.
Infeksi Larva Cacing Anisakis spp. pada Ikan Layur (Trichiurus lepturus) YUSTISIA SEMARARIANA, I WAYAN; OKA, IDA BAGUS MADE; ADI SURATMA, I NYOMAN
Indonesia Medicus Veterinus Vol 1 (2) 2012
Publisher : Indonesia Medicus Veterinus

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Penelitian ini menggunakan metode observasional dengan mengamati adanya larva cacing Anisakis spp. pada 32 ekor ikan layur (Trichiurus lepturus) yang ditangkap di perairan laut Kedonganan Badung. Dengan hasil yang didapatkan prevalensi infeksi larva cacing Anisakasis spp. pada ikan layur di perairan laut Kedonganan sebesar 31,25%. Pada ikan berukuran < 100 cm sebesar 26,67 % dan pada ikan berukuran > 100 cm sebesar 100%. Intensitas infeksi larva cacing Anisakis spp. pada ikan layur di perairan laut Kedonganan rata-rata 9,2 larva per ekor ikan, pada ikan berukuran < 100 cm dengan intensitas infeksinya rata-rata 2,63 larva per ekor ikan dan pada ikan berukuran > 100 cm intensitas infeksinya rata-rata 35,5 larva per ekor ikan. Terdapat kolerasi yang sangat nyata (p<0,01) antara ukuran ikan dengan intensitas infeksi. Distribusi larva cacing Anisakis spp. pada ikan layur adalah pada rongga abdomen, lambung, usus dan otot. Pada ikan berukuran < 100 cm distribusi infeksinya pada rongga abdomen dan usus. Pada ikan berukuran > 100 cm distribusinya infeksinya pada rongga abdomen, lambung, usus dan otot. Dengan presentasi adalah pada rongga abdomen 100%, lambung 10%, usus 50% dan otot 10%.
STRONGYLOIDOSIS PADA ANAK BABI PRA-SAPIH Oka, Ida Bagus Made; Dwinata, I Made
Buletin Veteriner Udayana Vol. 3 No.2 Agustus 2011
Publisher : Buletin Veteriner Udayana

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The Research was conducted to determine the contribution Strongyloides ransomi againstdiarrhea in piglets on pig farms inBali. The number of sample 501 piglets feces fromBadung, Tabanan and Gianyar. The examination of worm eggs with Sodium AceticFoemaldehid method (SAF). The data obtained were analyzed statistically with Fischer test/ Chi Qwadrat Test.The results was obtained the prevalence of Strongyloides ransomi in piglets of 7.4%. Theresults was found a significant relationship between infection Strongyloides ransomi withdiarrhea, where the infected piglets Strongyloides ransomi higher risk ( OR=6 ) than ofpiglets uninfected.
Seroprevalensi dan Isolasi Toxoplasma gondii pada Ayam Kampung di Bali (SEROPREVALENCE AND ISOLATION OF TOXOPLASMA GONDII AMONG FREE-RANGE CHICKENS IN BALI) Dwinata, I Made; Oka, Ida Bagus Made; Suratma, Nyoman Adi; Damriyasa, I Made
Jurnal Veteriner Vol 13, No 4 (2012)
Publisher : Jurnal Veteriner

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The prevalence of Toxoplasma gondii in free range chickens is a good indicator of the prevalence of T.gondii oocysts in the environment and the meat of chicken is considered one of the sources of the humaninfection. A study to determine the seroprevalence of T.gondii in free ranging chickens in eight regency inBali have been undertaken. More over, attempt to isolate T gondii was also performed from the copropositivesample. Seroprevalence was detected using modified agglutination test (MAT) and isolation of T.gondiiwere performed from organs (heart and brain) using pepsin-HCl digestion method. Further the pathogenicityof the isolate was determined by bioassay using mice. The result showed that the seroprevalence was24.8% (31 out of 125 chickens examined). T.gondii was found in 17 of the 31 seropositive chickens (55%)more over all isolates were a vitulent to the mice.
Protein Spesifik Cairan Kista Cysticercus bovis pada Sapi Bali yang Diinfeksi dengan Taenia saginata (SPECIFIC PROTEIN OF CYSTICERCUS BOVIS CYST FLUID ON BALI CATTLE EXPERIMENTALLY INFECTED WITH TAENIA SAGINATA) Dharmawan, Nyoman Sadra; Dwinata, I Made; Swastika, Kadek; Damriyasa, I Made; Oka, Ida Bagus Made; Astawa, I Nyoman Mantik
Jurnal Veteriner Vol 14, No 1 (2013)
Publisher : Jurnal Veteriner

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Cysticercus bovis is the larval stage of Taenia saginata, the bovine tapeworm. The infection of thislarval in cattle musculature causes Bovine cysticercosis or Cysticercosis bovis.  Bovine cysticercosis is foundworldwide, but mostly in developing countries, where unhygienic conditions, poor cattle managementpractices, and the absence of meat inspection are common.  The adult Taenia infection in man is referredto as taeniasis.  Taenia saginata taeniasis is also found almost all over the world.  The prevalence ofTaenia saginata taeniasis has reported up to 27.5% in Gianyar Bali. In order to control the diseases,vaccination against the larvae stages in cattle of Taenia saginata may play an important role in controllingthe disease in the endemic regions.  The aims of the present study were to prepare and to investigate theimmunogenic protein as vaccine candidate for controlling  Cysticercus bovis infection in in Bali cattle.Cysticercus protein from the cyst fluid was firstly used to immunize mice and the mice sera were thencollected. Cysticercus proteins then analyzed using sodium dodecyl sulfate-gel electrophoresis (SDS-PAGE).All cysticercus proteins were then visualized by Commasie blue staining. The proteins were also transferredonto nitrocellulose membrane and the immunogenic proteins were visualized by Western Blotting usingimmune sera raised in mice.  By Commasie blue staining, a total of 17 proteins were detected with themolecular weight of 14,86 kDa -122,40 kDa from the smallest to the largest. As many as 7 immunogenicproteins with the molecular weights of 16.81 kDa; 19.22 kDa; 20.98 kDa; 27.41 kDa; 34.02 kDa; 38.31 kDa;and 54.94kDa were detected.
Prevalensi dan Intensitas Infeksi Parasit Crustacea pada Ikan Sulir Kuning (Caesio cuning) dan Ikan Pisang-pisang (Pterocaesio diagramma) yang Dipasarkan Di Pasar Ikan Kedonganan, Kabupaten Badung Hambarsika, I Gusti Agung Made Armada; Oka, Ida Bagus Made; Suratma, Nyoman Adi
Buletin Veteriner Udayana Vol. 6 No.1 Pebruari 2014
Publisher : Buletin Veteriner Udayana

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Penelitian ini bertujuan untuk mengetahui prevalensi,  intensitas infeksi dan predileksi parasit crustacea pada ikan sulir kuning (Caesio cuning) dan Ikan Pisang-pisang (Pterocaesio diagramma) yang dipasarkan di Pasar Ikan Kedonganan, Kabupaten Badung. Penelitian ini dilakukan dengan menggunakan 35 sampel Ikan Sulir Kuning (Caesio cuning) dan 35 sampel Ikan Pisang-pisang (Pterocaesio diagramma) yang diperoleh di Pasar Ikan Kedonganan. Metode penelitian yang digunakan adalah metode observatif dan analisis data dengan uji Chi-Square. Hasil penelitian yang telah dilakukan, didapatkan prevalensi infeksi parasit Crustacea pada Ikan Sulir Kuning (Caesio cuning) adalah 22,86 %, dan pada Ikan Pisang-pisang (Pterocaesio diagramma) adalah 31,43 %. Parasit Crustacea yang menginfeksi Ikan Sulir Kuning (Caesio cuning) dan Ikan Pisang-pisang (Pterocaesio diagramma) adalah dari kelompok Isopoda dan Copepoda. Identifikasi lebih lanjut, ternyata kedua ikan ini terinfeksi jenis parasit Crustacea yang sama, yaitu Isopoda jenis larva praniza dan Copepoda jenis Sagum folium. Rerata intensitas infeksi  Sagum folium pada Ikan Sulir Kuning (Caesio cuning) adalah 1,5 ± 1,46 dan larva praniza adalah 1,5 ± 1,40. Rerata intensitas infeksi Sagum folium pada Ikan Pisang-pisang (Pterocaesio diagramma) adalah 2 ± 1,77 dan larva praniza adalah 1. Parasit Crustacea yang ditemukan berpredileksi pada insang, operculum dan mata. Setelah dilakukan analisis dengan Uji Chi-Square, ternyata tidak terdapat hubungan yang nyata antara jenis ikan dengan prevalensi infeksi parasit Crustacea.
Prevalensi Infeksi Cacing pada Ikan Pisang-pisang (Pterocaesio diagramma) dan Ikan Sulir Kuning (Caesio cuning) yang Dipasarkan di Pasar Ikan Kedonganan, Badung Pradipta, I Putu Hendra; Suratma, Nyoman Adi; Oka, Ida Bagus Made
Buletin Veteriner Udayana Vol. 6 No.1 Pebruari 2014
Publisher : Buletin Veteriner Udayana

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Penelitian ini bertujuan untuk mengetahui prevalensi, intensitas infeksi serta distribusi cacing pada berbagai organ, selain itu dapat juga  melihat hubungan antara jenis ikan dengan prevalensi infeksi cacing pada ikan Pisang - pisang (Pterocaesio diagramma) dan ikan Sulir Kuning (Caesio cuning)  yang dipasarkan di Pasar Ikan Kedonganan, Badung. Metode penelitian yang dilakukan adalah dengan pengamatan secara makroskopis dan mikroskopis mengikuti metode Fernando et al. & Kabata, kemudian data dianalisa secara deskriptif dan menggunakan uji Chi-Square.  Prevalensi dan intensitas dari masing-masing jenis cacing pada ikan Pisang – pisang (Pterocaesio diagramma) yaitu digenea (45,71%) dengan rata-rata 4,81±5,9, cestoda (34,29%) dengan rata-rata 12,5±18,1, Hysterothylacium sp. (2,86%) dan Raphidascaris sp. (2,86%) ditemukan berjumlah 1 ekor, Terranova sp. (8,57%) dengan intensitas 1 ekor setiap ikan,  acanthocephala (42,85%) dengan rata-rata 2,13±1,35, sedangkan ada 2 ekor cacing (5,71%) tidak bisa teridentifikasi. Pada ikan Sulir Kuning (Caesio cuning) dari 35 ekor ikan yang diteliti , prevalensi digenea (82, 86%) dengan rata-rata 5,62±4,6, Hysterothylacium sp. (5,71%) dengan rata-rata jumlah cacing 1 ekor dan Cucculanus sp (5,71%) dengan rata-rata 1,5. Lokasi distribusi cacing yang menginfeksi ikan Pisang - pisang (Pterocaesio diagramma) dan ikan Sulir Kuning (Caesio cuning) ditemukan pada beberapa organ antara lain operkulum, insang, rongga insang, lambung, usus, hati, sekum, gonad dan rongga tubuh. Setelah dilakukan analisis statistik menggunakan uji Chi-Square ternyata prevalensi infeksi tidak berhubungan nyata (P>0,05) dengan jenis ikan.
Infeksi Cacing Saluran Pencernaan Monyet Ekor Panjang (Macaca fascicularis) Yang Diperdagangkan Di Pasar Satria Denpasar Dwipayanti, Kadek Ari; Oka, Ida Bagus Made; Rompis, Aida LT
Buletin Veteriner Udayana Vol. 6 No.1 Pebruari 2014
Publisher : Buletin Veteriner Udayana

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Penelitian ini bertujuan untuk mengetahui jenis dan intensitas serta prevalensi infeksi cacing saluran pencernaan pada monyet ekor panjang (Macaca fascicularis) yang diperdagangkan di Pasar Satria Denpasar. Empat puluh lima sampel feses M. fascicularis (24 ekor monyet betina dan 21 ekor monyet jantan) diambil secara aseptis, diberi label dan selanjutnya diperiksa di laboratorium Parasitologi FKH Universitas Udayana. Sampel diperiksa menggunakan metode konsentrasi sedimentasi untuk mengidentifikasi jenis cacing dan modifikasi McMaster untuk mengetahui intensitas infeksi. Jenis cacing saluran pencernaan yang berhasil diidentifikasi berturut-turut adalah Ancylostoma sp. (91,1%), Trichostrongylus sp./Oesophagostonum sp. (73,3%), Trichuris sp. (22,2%), Ascaris sp. dan Taenia sp. (4,4%). Rataan intensitas infeksi cacing      Ancylostoma sp. 4913 ± 4849 telur per gram tinja, Thrichostrongylus sp./ Oesophagostonum sp. 871 ± 816 telur per gram tinja, Trichuris sp. berkisar      171 ± 111 telur per gram tinja. Ascaris sp. dan Taenia sp. memiliki intensitas terendah yakni <100 telur per gram tinja. Secara umum prevalensi kecacingan adalah 93,3%. Prevalensi kecacingan pada monyet betina (52,2%) sedangkan pada monyet jantan (48,8%). Berdasarkan umur, prevalensi kecacingan pada anakan, bayi, dan dewasa berturut-turut (72,1%), (25,6%), dan (2,3%). Pola infeksi umumnya tipe infeksi campuran (82,3%) dibandingkan infeksi tunggal (17,7%).