ARIYANTI OETARI
Department of Biology, Faculty of Mathematics and Natural Sciences, University of Indonesia, Depok 16424

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Ecological and Taxonomical Perspective of Yeasts in Indonesia SJAMSURIDZAL, WELLYZAR; OETARI, ARIYANTI; KANTI, ATIT; SARASWATI, RASTI; NAKASHIMA, CHIHARU; WIDYASTUTI, YANTYATI; KATSUHIKO, ANDO
Microbiology Indonesia Vol 4, No 2 (2010): August 2010
Publisher : Indonesian Society for microbiology

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Abstract

In the course of ecological and taxonomical study of yeasts in Indonesia, a total of 2147 yeast isolates from 315 samples in the year 2003, 2005, 2006, and 2008 had been obtained from eight locations e.g. Liwa (Sumatera), Cibinong (Java), Cibodas (Java), Kutai (Kalimantan), Enrekang (Sulawesi), Pucak (Sulawesi), Gili and Kuta (Lombok), and Kupang (Timor).  Leaves, flowers, litters, soils, epiphytic soils, insects and insect´s nests were collected for yeasts isolation.  Our molecular identification based on D1/D2 region of nuclear large-subunit rDNA and the internal transcribed spacer (ITS) regions sequence data on 525 representative isolates revealed that 306 isolates belong to 48 described species (18 genera) and 209 strains belong to 19 undescribed species (19 genera), and 10 isolates were discarded because of contamination.  Based on their substrates, litter had the highest yeasts genera (19) followed by soils (18), flowers (10), leaves (6), epiphytic soils (4), and insects and insect´s nests (4).  Genera found on soils were also common on litters.  Yeasts genera found on flowers and epiphytic soils were common on leaves and litters.  The genera Aureobasidium, Cryptococcus, Pseudozyma, Rhodotorula and Sporidiobolus were found in all substrates.  Based on their locations, Kutai had the highest number of genera (15) followed by Cibodas (10), Cibinong (10), Enrekang (10), Kupang (10), Pucak (9), Liwa (7), and Lombok (7).  The genus Cryptococcus was found in all locations.  Our study shed a light to detection of many new taxa of yeasts, 41% of yeasts found in this study represented novel taxa.
PENGUJIAN KEMAMPUAN ANTAGONISTIK KHAMIR Rhodotorula spp. ASAL KEBUN RAYA CIBODAS TERHADAP KAPANG DARI TANAMAN TOMAT TERINFEKSI DENGAN CO-CULTURE Hafsari, Anggita R; Oetari, Ariyanti; Salamah, Andi; Sjamsuridzal, Wellyzar
JURNAL ISTEK Vol 5, No 1-2 (2011): ISTEK
Publisher : JURNAL ISTEK

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Aspergilus ochraceu and A. terreus had been known as mould that causes significant loss to quality of tomatoes. One of potential methods to control the development of this mould by using the antagonistic activity of Rhodotorula sp. Six strains of Rhodotorula sp., UICC Y-318, UICC Y-325, UICC Y- 332, UICC Y-381, UICC Y-384, and UICC Y-386, which isolated from Cibodas Botanical Garden, West Java were observed for its antagonistic activity against mould A. ochraceu D1.2.2.2.SSM3 and A. terreus D2.2.MC. Co-culture method was used as 1.58 to 5.59 x 108 CFU/ml Rhodotorula sp. growth together with 7 x 107 CFU/ml A. ochraceus D1.2.2.2.SSM3 and 7.5 x 107 CFU/ml A. terreus D2.2.MC at PDB. Result showed that the highest percentage reduction of conidial heads and hyphal width was show by Rhodotorula sp. UICC Y-318 against A.ochraceus (9.45% and 12.43%; 7.10% and 7.51%, 2 and 3 day after incubation, respectively).
Penapisan Aktivitas Selulase Isolat-isolat Khamir dari Moluska, Serasah, dan Tumbuhan di Taman Nasional Gunung Halimun, Jawa Barat Mangunwardoyo, Wibowo; Fitri, Reno; Oetari, Ariyanti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 3 (2007): October 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i3.2805

Abstract

A total of 236 yeast isolates from mollusc, litter, and plant samples from Gunung Halimun National Park were screened for cellulolytic activity based on Smith method by using 0,2% (w/v) cellulose-azure for 30 days. The results showed that 12 isolates (9 isolates from plants, 2 isolates from molluscs, dan 1 isolate from litter) have cellulolytic activity. These isolates were further screened based on Teather and Wood method for six days to determine their cellulases components by using specific substrates. Carboxymethyl cellulose (CMC) 0,1% (w/v) was used as a specific substrate to determine endoglucanase activity. Avicel 0,1% (w/v) was used as a specific substrate to determine exoglucanase activity. Cellobiose 0,1% (w/v) was used as a specific substrate to determine ?-glucosidase activity. The results showed that 6 isolates from plant have ?-glucosidase activity, and 1 isolate from plant have ?-glucosidase and endoglucanase activities. Five isolates (2 isolates from plants, 2 isolates from molluscs, and 1 isolate from litter) showed no cellulase activity on specific substrates after six days incubation.