Nursyirwani Nursyirwani
Jurusan Ilmu Kelautan Fakultas Perikanan dan Ilmu Kelautan Universitas Riau Kampus Bina Widya Sp.Panam Pekanbaru-Riau-Indonesia. Telp.0761-862620 E-mail: nursyirwani_adnan@yahoo.com

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Journal : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

ANTAGONISM HETEROTROPHIC BACTERIA ISOLATED FROM ESTUARY OF SIAK RIVER AND SETTLED SEA OF SIAK REGENCY AGAINST PATHOGENIC BACTERIA Zirma, Andrei Putra; Feliatra, Feliatra; Nursyirwani, Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5, No 1 (2018): Wisuda April Tahun 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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   Heterotrophic bacteria is bacteria that use organic substances as its nutrition sources. Organic substances are obtained from the rest of other organisms, waste or substances contained in the body of other organisms. This study aims to determine the activity of heterotrophic bacteria against pathogenic bacteria (Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas sp.). Total amount of bacteria at station 1 was higher than station 2, those were 2.3 x 107 cfu / ml at station 1 and 1.8 x 107 cfu / ml at station 2. Statistical analysis using the simple linear regresion indicated that the correlation between the growth of heterotrophic bacteria on environmental parameters (DO, pH, temperature and salinity) was very weak of wich correlation value(r) was 0.20  for all parameters. Antagonism test against pathogenic bacteria found that there were 8 bacterial isolates wich potentially inhibited the growth of the three pathogenic bacteria, the isolates were A11, A12, A14, A19, A21, A22, A23 and A25 with the inhibition zone ranges 4,8 - 14,5 mm. From the DNA sequences with 16S rDNA method and BLAST analysis, the bacteria isolates were identified asKerstersia gyiorum, Kerstersia sp.,Enterococcus sp. and three isolates was unidentified possibly due to these species hadnot registered in GenBank or had never been identified beforeKey words: Heterotrophic Bacteria, Antagonism, Estuary, 16s rDNA
ANALYSIS OF METAL CONTENT OF Pb, Cr, Cu, AND Zn IN GONGGONG SNAIL (Strombus canarium) FROM COASTAL WATERS OF SINGKEP ISLAND LINGGA REGENCY OF RIAU ARCHIPELAGO PROVINCE Nurdiansyah, Meiji; Amin, Bintal; Nursyirwani, Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 1 Januari s/d Juni 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT This research was conducted in December 2017 in the coastal waters of Singkep Island, Lingga Regency, Riau ArchipelagoProvince. The purpose of this research was to analyse the content of metal Pb, Cr, Cu and Zn on soft tissue in gonggong snail (S. canarium) with different size and from two different stations. This study also determine the status of heavy metal pollution based on MPI (Metal Pollution Index) value and the feasibility consumption of gonggong snail. Analysis of metal content of heavy metal based on Yap et al., (2003). The results showed that the highest metal content of Pb was found in large snail size (1.1827 µg/g) at Station 2 and the highest Cr content was found in medium-sized snails (1.4053 µg/g) at Station 2. Highest Cu (27.9133 µg/g) in Station 1 and the highest Zn content was foundin large snail size (52.3273 µg/g) at Station 1. Average Cu and Zn content (23.7581 µg/g and 48.2146 µg/g) was higher than that of Pb and Cr (0.7800 µg/g and 1.0089 µg/g) in snails soft tissues. The MPI value of Singkep Island waters was 5.48 which was still relatively low. Based on the calculation of PTWI, gonggong snail in Singkep Island waters was still safe and feasible to be consumed for not exceeding the safe limits of consumption that has been determined, these are for Pb (8.9755 kg/week), Cr (6.3832 kg/week), Cu (41.2491 kg/week), and Zn (40.6516 kg / week). Key Words: Singkep Island, Heavy Metal, Pollution, Gonggong Snail (Strombus canarium)
DEGRADATION ABILITY AND IDENTIFICATION OF OIL DEGRADATION BACTERIA BY 16S rRNA SEQUENCING Shaumi, Nurul; Nursyirwani, Nursyirwani; Feliatra, Feliatra
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 1 Januari s/d Juni 2018
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ABSTRACTPollution in the marine environment caused by industrial activity, marine transportation, offshore mining and oil refineries, one of which comes from petroleum contamination. One of  efforts to prevent and to manage oil pollutionwas by the use of oil degrading bacteria. The objective of this research was to examine degradation ability of marine bacteria isolates and identify the bacterial isolate based on 16S rRNA sequence. The research was conducted from March to May 2018. The oil degradation analysis was conducted at Laboratory of Marine Microbiology,Faculty of Fishery and Marine Science, while the molecularidentification of bacteria was done in Genetics Laboratory, Faculty of Mathematics and Natural Sciences, University of Riau. The results showed that five isolates of oil degradation bacteria, selected(BM 1d, BM 7b, BM 7c, BM 14a and BM 14b) were able to degrade crude oil at different concentrations. The highest degradation at 1% concentration is indicated by BM 14b isolate, at 2% concentration was indicatedby BM 1d isolate and at 3% concentration was indicated BM 14b isolate. From the degradation analysis results, three isolates (BM 1d, BM 14a, BM 14b) have the highest degradation ability to determine the type of oil degrading bacteria. The molecular identification results by the 16S rRNA sequencing and BLAST analysis showed that BM 1d isolate were similar to Bacillus sp. (95%), BM 14a isolate were similar to Bacillus flexus (92%) and BM 14b isolate were similar to Bacillus cereus (98%). Keyword : Oil Degradation Bacteria , Crude Oil, 16S rRNA, Bacillus
ISOLATION OF OIL DEGRADATION BACTERIA FROM SEDIMENT IN SUNGAI PAKNING WATERS OF BENGKALIS REGENCY AND THE ABILITY TO DEGRADE CRUDE OIL Fitria, Gina Ulfa; Nursyirwani, Nursyirwani; Thamrin, Thamrin
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
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ABSTRACT Oil pollution in Bengkalis waters usually comes from land, marine transportation, defection port activities and oil industry. Oil pollution can be trapped in sediment because it can not be dissolved and difficult to degrade, one way to overcome pollution is by bioremediation. The purpose of this research was to isolate bacteria from sediment Sungai Pakning waters, to examine the ability of isolates in degrading crude oil and to identify the bacterial isolates genetically based on sequence 16S rRNA. The research was conducted from March to May 2018. Bacterial isolation was conducted in Laboratory of Marine Microbiology and the oil degradation analysis was conducted in Laboratory of Chemical Oceanography, Faculty of Fishery and Marine Science, University of Riau. This research used survey and experiment method, survey method to measuring of water quality, removal of sample and isolated of bacteria, experiment method to examine the ability of isolates in degrading crude oil. The result showed that eight isolates (7D15, 7D35, 7D23, 14D15, 14D34, 21D13, 21D23, 21D35) were able to degrade crude oil at 1-3% concentrations. The highest degradation at 1% concentration was indicated by 21D35 isolate, at 2%  concentration is indicated by  21D13 isolate, and at 3% concentration is indicated by 7D23 isolate. To molecular identification results by the 16S rRNA sequence take of three isolates have the highest degradation ability. 21D13 isolate was similar to Achromobacter pulmonis, 21D35 isolate was similar to Achromobacter sp., and 7D23 isolate was similar to Vibrio sp. Keyword : Pakning River, Oil Degradation Bacteria, 16 rRNA, Achromobacter, Vibrio
CHARACTERIZATION OF LACTIC ACID BACTERIA FROM CINCALUK BASEDON16S rRNA SEQUENCE Ayu, Putri; Nursyirwani, Nursyirwani; Feliatra, Feliatra
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 1 Januari s/d Juni 2018
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ABSTRACT Lactic acid bacteria (LAB) is a groupof bacteria frequently used as probiotic in aquaculture because of its beneficial effects on health, pathogenic bacteria prevention and increase of fish growth. LAB could be found in fermented fish products such as Cincaluk that is produced by people in Tembilahan and Bengkalis of Riau Province. The purpose of this research was to characterize LAB isolated from Cincaluk Tembilahan and Bengkalis genetically. This research was conducted from February until May 2018. Bacterial isolates were identified based on morphology, biochemical characters and 16S rRNA sequence. 24 isolates found were round, creamy white, Gram positive, did not produce indole, produced catalase, non motil, used citrate, did Methyl Red, produced H2S and gas, glucose, sucrose and lactose were fermented. Based on 16S rRNA sequence. Five of six isolates selected were identified as Lactobacillus plantarum (97-100% similarites) and one isolate was Bacillus cereus (94%) Keyword:Characteristic, Lactid Acid Bacteria, Cincaluk, Genetic,16S rRNA sequence 
Inhibitory Powerof Lactic Acid Bacteria (BAL) from Cincaluk against pathogenic bacteria (Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus)and protease enzyme production Hutabarat, Povy; Nursyirwani, Nursyirwani; Effendi, Irwan
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
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ABSTRACT Lactic acid bacteria (BAL) have the ability to inhibit the growth of pathogenic bacteria. BAL is bacterium that has an important role in the processof fermentation of organic materials. BAL can be found in traditional fishery products such as cincaluk (fermented shrimp). This study was conducted from February to April 2018. Test of isolate activity of BAL and protease enzyme was carried out at Marine Microbiology Laboratory, Faculty of Fisheries and Marine Scince, University of Riau.The study aimed was to examine the BAL activity of cincaluk against pathogenic bacteria and tested the protease enzyme produced by BAL from the cincaluk produced the community in Bengkalis and Tembilahan, Riau. This research used survey method where fresh cincaluk sample was used as abservation object in isolating the BAL and testing antagonism against three different pathogenic bacteria (Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus). The results of antagonism test showed that all bacterial isolated have ability to inhibit the growth of the three pathogenic bacteria. The highest inhibition zone P.aeruginosawas demonstrated by a GT isolate (11.0 mm), toward the highest inhibition zone against E. coliwas indicated by PB isolate (8.7 mm), and toward S. aureuswasdemonstrated by MB isolate (6.6 mm). All bacterial isolates produced protease enzyme indicated by clear zone around the colonies on skim milk medium. Keywords: BAL, Antagonism, Inhibition zone, Pathogens, Protease    
DENSITY OF Escherichia coli BACTERIA AND HETEROTROPHIC BACTERIA IN DUMAI SEAWATER OF RIAUPROVINCE Qoriman, Anisa; Feliatra, Feliatra; Nursyirwani, Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
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ABSTRACT This research was conducted from April until May 2018. The purpose of this study was to calculate the density of Escherichia coli in sea water of Dumai, to determine the ratio of E. coli to heterotrophic bacteria and to examine the resistance of E. coli to antibiotics. In this study the determination of sampling points using purposive sampling method, was set intothree sampling points for each station. Density ofE.coliwas calculated by using the Most Probable Number (MPN) method and density total of heterotrophic bacteria by using spread plate count method. The result showed that densityE. colirangedfrom 2.37 x 104 – 1.1 x 105 CFU/ml. The highest MPN number ofE. coliwas found in stations 2 and 3, andthe lowest wasin station 5. The total number of heterotrophic bacteria ranged from 7.37 x 106 – 10.38 x 106 CFU/ml. The highest number of heterotrophic was foundin station 4 and the lowest was in station 5.The highest percentage ratio of density of E.colito heterotrophic bacteriawas in station 2(1.20%)and the lowest was in station 5 (0.32%).Resistance of E.coli toward chloramphenicol as antibiotic testedindicated that 7 isolateswere resistant with the inhibition zone ranged from 2.17 –10.70 mm, 9 isolates were categorized intointermediate with the inhibition zone ranged from 11.90–19.33 mm and 1 isolate was sensitive with the inhibition zone was 22.77 mm. Towardantibiotics penicillin andisoniazid, all bacterial isolates were resistant with the inhibition zone ranged from 0.80 - 4.70 mm and 1.50- 4.67 mm, respectively.Keyword: Density, Escherichia coli,MPN method, Heterotrophic Bacteria, Antibiotics 
ISOLATION OF DIESEL OIL DEGRADATION BACTERIA IN THE WATERS AROUND THE PORT OF TELUK BAYUR PADANG CITY Citra, Sri Melinda; Yoswaty, Dessy; Nursyirwani, Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
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ABSTRACT Diesel oil degradation bacteria can be found in various habitats in the marine environment including in the waters around the harbor. This research aims at diesel oil in the waters around the port of Teluk Bayur and test its ability to degrade diesel oil. Isolation of the degrading bacteria has been from April to conductedJune 2018 in the Marine Microbiology Laboratory, Department of Marine Science, Faculty of Fishery and Marine University of Riau.Total count of diesel oil decomposing bacteria ranges from 0.4 x 106 to 7.3 x 106 CFU/ml. Eleven isolates of the diesel oil degradation bacteria were selected, ie BM1a, BM1b, BM1c, BM1d, BM7a, BM7b, BM7c, BM7d, BM14a, BM14c and BM14d. Based on morphological, physiology and biochemical characteristics, seven isolates were irregular, two round and concentric isolates, seven isolates with corrugated colony edges, two irregular and notched isolates, six yellow colony isolates, five white isolates, colony elevation all isolates emerge, all isolates were positive on catalase test, in H2S test all negative isolates, all isolates in the sugar test produced gas, all isolates were motile and Gram negative, all indole isolates were negative, seven methyl red positive isolates and four methy red isolates negative. In the degradation test only six bacterial isolates were selected, ie BM1a, BM1c, BM7a, BM7d, BM14c and BM14d. The highest degradation of diesel oil at concentrations 1%, 2% and 3% were performed by isolates BM1c, BM7a and BM1a respectively. Overall, all isolates were able to degrade diesel oil in the waters around Teluk Bayur Port, Padang City. Keyword: Isolation, Bacteria, Degradation, Diesel Oil 
ABUNDANCE OF Anabaena azollae AND NITRATE LEVELS IN Azolla microphylla BRACKISH WATER CULTURE Nanggola, Nursyafni; Effendi, Irwan; Nursyirwani, Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
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ABSTRACT               Azolla michrophylla is beneficial for Anabaena azollae because it can act as a host through the supply of oxygen and nitrogen that can be used. Nitrogen fixation carried out by A. Azollae is atmospheric nitrogen gas (N2) converted into ammonia (NH3). This research was conducted at the Laboratory of Marine Microbiology and Marine Chemistry, Department of Mariene Science, Faculty Of Fisheries And Marine Science, University of Riau in April to June 2018. The purpose of this research was to determine the abundance of A. azollae in the growth of A. microphylla plants and determine the levels of nitrate contained in the growth of A. microphylla in brackish water culture. Factorial randomized group design (RAK) was applied in this experimental. Temperature, salinity, and pH were water quality parameters measured. The results showed that the best growth of A. microphylla was obtained at a salinity of 0 ppt with a starter of         A. microphylla 3 gram with a value of 50.42 gram. The highest abundance of A.  azollae is found in salinity 3 ppt with a 3 gram level with the abundance of 5643 cells / ml. A. microphylla and A. azollae had sufficient nitrate levels for their growth in brackish water cultured at 0, 1, 2, and 3 ppt salinitis of in combination with  A. microphylla 1, 1.5 and 3 gram as a starters. The highest nitrate level was obtained on the 14th and 21st day with a value of 1.2625 mg / L and 1.4500 mg / L.   Keywords: Azolla microphylla, Anabaena azollae, Growth, Abundance, Nitrate.