Nursyirwani Nursyirwani
Jurusan Ilmu Kelautan Fakultas Perikanan dan Ilmu Kelautan Universitas Riau Kampus Bina Widya Sp.Panam Pekanbaru-Riau-Indonesia. Telp.0761-862620 E-mail: nursyirwani_adnan@yahoo.com

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Isolasi dan Karakterisasi Bakteri Hidrokarbonoklastik dari Perairan Dumai dengan Sekuen 16S rDNA

ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 12, No 1 (2007): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Penelitian ini dilaksanakan dari bulan September sampai Desember 2006. Tujuan penelitian adalah untuk mempelajari karakteristik molekuler bakteri hidrokarbonuklastik yang diisolasi dari perairan laut Dumai berdasarkan sekuen 16S rDNA. Isolasi bakteri dilakukan pada media cair dan padayang ditambahk an Sumatran crude oil. Karakteristik molekuler diperoleh melalui isolasi dan ampHdkasi DNA dengan PCR dan sekuensing menggunakan ABI 3130 Genetic Analyzer. Dari 6 isolat yang danalisis, hanya ada 3 isolatyang dapat disekuensing. Dari perbandingan dengan BLAST database, didapatkan kesamaan sekuen yang terdekat untuk isolat CA (91%) adalah Providenda vermicola, isolat DA (93%) adalah Burkholderia cepacia, dan isolat FA (99%) adalah Myroides odoratimimus.Kata kunci: Bakteri hidrokarbonuklastik, PCR dan 16S rDNAThe research was conducted from September to December 2006. The aim was to study molecular characterization of hidrocarbonoclastic bacteria based on sequence 16S rDNA from Dumai waters. The bacteria was isolated in both broth and solid media added with the Sumatran crude oil. Molecular characterization included DNA isolation and amplification using PCR, and sequencing by ABI 3130 Genetic Analyzer. Three of six isolateswere successfully sequenced. The comparison of 16S rDNA with known 16S rDNA sequences from BLAST database showed that the closest sequence similarity of isolate CA (91%) was Providencia vermicola, isolate DA(93%) was Burkholderia cepacia, and isolate FA (99%) was Myroides odoratimimus.Key words : Characterization, Hydrocarbonoclastic Bacteria, PCR, 16S rDNA

Isolasi Bakteri Asam Laktat dari Usus Ikan Kerapu Macan (Epinephelus fuscoguttatus) dan Potensinya Sebagai Antivibrio

ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 16, No 2 (2011): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Tujuan penelitian ini adalah untuk mengisolasi dan menguji potensi antibakterial bakteri asam laktat dari ikan Kerapu Macan (Epinephelus fuscoguttatus) terhadap bakteri patogen Vibrio alginolyticus. Bakteri tersebut diisolasi dari usus ikan dengan metode sebarulas pada media agar MRS dan GYP+CaCO3. Koloni yang tumbuh diidentifikasi  berdasarkan  karakter  morfologi,  biokimia  dan  fisiologi.  Aktivitas  antibacterial  terhadap  V. alginolyticus diuji dengan metode difusi kertas cakram pada media Zobell 2216E lapis ganda, diameter zona jernih disekitar kertas cakram diukur dengan kaliper. Pada medium agar GYP+CaCO3 didapatkan 21 isolat yang menampakkan zona jernih disekitar koloninya. Dari karakteristik morfologi, biokimia dan fisiologi dari isolat-isolat tersebut semua isolat dapat dikategorikan kedalam genus Lactobacillus. Tetapi, dari uji aktivitas antivibrio hanya 20 isolat menunjukkan zona hambat yang berkisar dari 14,0-21,5 mm, dimana tiga isolat menunjukkan aktivitas tertinggi, yaitu berturut-turut isolat KSBU 12C, KSBU 13D dan KSBU 5Da.Kata kunci: Isolasi, bakteri asam laktat, antivibrio, Kerapu Macan The objectives of this research were to isolate and examine antibacterial potency of lactic acid bacteria from Tiger Grouper (Epinephelus fuscoguttatus) against pathogen, Vibrio alginolyticus. The bacteria were isolated from the fish intestine by spread plate method on MRS and GYP+CaCO3  agar media. The grown colonies were identified based on morphological, biochemical and physiological characters. Antibacterial activity against V. alginolyticus was examined by the paper disc diffusion method on Zobell 2216E double layer agar, and clear zone diameter around the paper disc was measured by using caliper. Twenty one isolates  with  clear zone around the colonies were obtained from the GYP+CaCO3 agar. Morphological, biochemical and physiological characteristics of the colonies and cells indicated that all isolates might be categorized into Lactobacillus. However, there were only twenty isolates showed inhibition zones from 14.0-21.5 mm in antivibrio activity test, of which the highest activity was indicated by three isolates namely KSBU12C, KSBU 13D and KSBU 5Da, respectively. Key words: Isolation, lactic acid bacteria, antivibrio, Tiger grouper.

Properti Probiotik Isolat Bakteri Asam Laktat untuk Mengendalikan Pertumbuhan Vibrio alginolyticus pada Ikan Kerapu Macan (Epinephelus fuscoguttatus)

ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 16, No 3 (2011): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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  Tujuan penelitian ini adalah untuk menyeleksi isolat bakteri asam laktat (BAL) yang telah diisolasi dari ikan Kerapu Macan (Epinephelus fuscoguttatus) berdasarkan toleransi terhadap pH dan bile salts, patogenisitas dan kemampuannya menekan pertumbuhan Vibrio alginolyticus pada uji ko-kultur. Hasil penelitian menunjukkan bahwa dua puluh isolat BAL yang telah diuji antagonismenya terhadap V. alginolyticus pada penelitian terdahulu, dapat hidup pada pH rendah 3 dan tumbuh pada konsentrasi bile salts 0,5% kecuali isolat KSBU 13D.  Semua isolat tidak patogen terhadap ikan Kerapu Macan setelah diinfeksi BAL pada konsentrasi 107 cfu/ml secara intraperitoneal (IP). Pada uji ko-kultur, dari 6 isolat BAL yang dipilih, tiga isolat (KSBU 12C, KSBU 5Da, KSBU 9) berturut-turut mampu menekan pertumbuhan V. alginolyticus, dan penambahan konsentrasi BAL terbaik adalah 107 cfu/ml. Kata kunci: bakteri asam laktat, vibriosis,  patogenisitas, ko-kultur, kerapu macan The research objective was to select lactic acid bacteria which previously isolated from Tiger Grouper (Epinephelus fuscoguttatus) based on pH and bile salts tolerances, pathogenicity test and ability to decrease the growth of Vibrio alginolyticus in co-culture test. The result shows that twenty isolates which had also been examined their antagonisms against V. alginolyticus previously, were able to survive relatively low pH  values 3 and able to survive at high bile salts concentrations 0.5% except KSBU 13D isolate.  All isolates were nonpathogenic to Tiger Grouper after being intraperitoneally infected with the lactic acid bacteria at concentration 107 cfu/ml. In co-culture test, V. alginolyticus was reduced significantly by three isolates (KSBU 12C, KSBU 5Da, KSBU 9) from 6 isolates selected, and the best additional bacterial concentration was  107 cfu/ml. Key words: lactic acid bacteria,  vibriosis,  pathogenicity,  kerapu macan

ANTAGONISM HETEROTROPHIC BACTERIA ISOLATED FROM ESTUARY OF SIAK RIVER AND SETTLED SEA OF SIAK REGENCY AGAINST PATHOGENIC BACTERIA

Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5, No 1 (2018): Wisuda April Tahun 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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   Heterotrophic bacteria is bacteria that use organic substances as its nutrition sources. Organic substances are obtained from the rest of other organisms, waste or substances contained in the body of other organisms. This study aims to determine the activity of heterotrophic bacteria against pathogenic bacteria (Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas sp.). Total amount of bacteria at station 1 was higher than station 2, those were 2.3 x 107 cfu / ml at station 1 and 1.8 x 107 cfu / ml at station 2. Statistical analysis using the simple linear regresion indicated that the correlation between the growth of heterotrophic bacteria on environmental parameters (DO, pH, temperature and salinity) was very weak of wich correlation value(r) was 0.20  for all parameters. Antagonism test against pathogenic bacteria found that there were 8 bacterial isolates wich potentially inhibited the growth of the three pathogenic bacteria, the isolates were A11, A12, A14, A19, A21, A22, A23 and A25 with the inhibition zone ranges 4,8 - 14,5 mm. From the DNA sequences with 16S rDNA method and BLAST analysis, the bacteria isolates were identified asKerstersia gyiorum, Kerstersia sp.,Enterococcus sp. and three isolates was unidentified possibly due to these species hadnot registered in GenBank or had never been identified beforeKey words: Heterotrophic Bacteria, Antagonism, Estuary, 16s rDNA

Daya Hambat Ekstrak Kuda Laut (Hippocampus spinosissimus) terhadap Pertumbuhan Bakteri Vibrio alginolyticus

Jurnal Perikanan Dan kelautan Vol 22, No 2 (2017)
Publisher : Fakultas Perikanan dan Ilmu Kelautan Universitas Riau

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Tujuan dari penelitian ini adalah menguji daya hambat ekstrak kuda laut(Hippocampus spinosissimus) terhadap pertumbuhan bakteri Vibrio alginolyticus.Penelitian ini dilakukan pada bulan Desember 2016 sampai Januari 2017 di LaboratoriumMikrobiologi Laut Jurusan Ilmu Kelautan, Universitas Riau. Ekstrak etanol kudalaut memiliki kandungan kimia steroid/triterpenoid, saponin dan glikosida sertamemiliki efek sitotoksik Uji daya hambat dilakukan dengan metode eksperimen menggunakanempat konsentrasi ekstrak (100%, 50%, 25% dan 12,5%). Dari hasil penelitianyang dilakukan ditemukan bahwa rata-rata diameter zona daya hambat ekstrakkuda laut tertinggi terdapat pada konsentrasi 100% (3,67 mm) sedangkan yang terendahterdapat pada konsentrasi 25% (0,73%). Ekstrak kuda laut mampu menghambatpertumbuhan bakteri V. alginolyticus dengan potensi lemah dengan nilai rata-rata diameterzona daya hambat <5 mm. Ekstrak kuda laut cenderung bersifat bakteriostatik.

Correlation Analysis Between Losses with Boiler Opacity and Ambient Air Quality Effect on Capacity of Pulmonary Function of Palm Oil Mill Employees (Comparative Study on PT. AIP and PT. BNS)

Jurnal Ilmu Lingkungan Vol 11, No 1 (2017)
Publisher : Jurnal Ilmu Lingkungan

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The comparative research was conducted from October 2016 until January 2017 at two companies namely PT. AIP Teluk Siak Factory (TSF) and PT. BNS Mandah Factory (MDF) in Riau Province. The purposes of the research were to analyze the correlation between oil and palm kernel (losses) that is entrained fuel (fibers and shells) with opacity emissions boiler in TSF and MDF, and to analyze the impact of ambient air SO2 levels on capacity pulmonary function palm oil mill employees (TSF and MDF) with controll variables personel protective aquipment, age, smoking habits, and tenure.Based on the results, The correlation strength of oil loss in fiber cyclone, kernel loss in fiber cyclone, and kernel loss in winowing with boiler opacity in TSF and MDF is low correlation (R value of 0.320). The simultaneous and partial oil loss in fiber cyclone, kernel loss in fiber cyclone, and kernel loss in winowing were not significantly affect the boiler opacity in both factories (F test and t test of significance  > 0.05). R2 value of 10.2% indicates the magnitude of losses effect on opacity. This analysis explains that the losses are not the dominant factor affecting the boiler opacity. The boiler opacity is influenced by quality and balance fuel with oxygen, continuity process in mill and optimization of boiler performance as the operation practice, maintenance and boiler sparepart replacement. The impact of ambient air SO2 levels on capacity pulmonary function when controlled by age and tenure showed significant values (p < 0.05). Age and tenure are factors that differentiate magnitude of the impact of ambient air SO2levels on capacity pulmonary function. While the impact of ambient air SO2 levels on capacity pulmonary function when controlled by personel protective aquipment (PPE) and smoking habits did not show significant values (p > 0.05) in both factories. PPE and smoking habits are not factors that differentiate magnitude of the impact of ambient air SO2 levels on capacity pulmonary function.

ANALYSIS OF METAL CONTENT OF Pb, Cr, Cu, AND Zn IN GONGGONG SNAIL (Strombus canarium) FROM COASTAL WATERS OF SINGKEP ISLAND LINGGA REGENCY OF RIAU ARCHIPELAGO PROVINCE

Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 1 Januari s/d Juni 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT This research was conducted in December 2017 in the coastal waters of Singkep Island, Lingga Regency, Riau ArchipelagoProvince. The purpose of this research was to analyse the content of metal Pb, Cr, Cu and Zn on soft tissue in gonggong snail (S. canarium) with different size and from two different stations. This study also determine the status of heavy metal pollution based on MPI (Metal Pollution Index) value and the feasibility consumption of gonggong snail. Analysis of metal content of heavy metal based on Yap et al., (2003). The results showed that the highest metal content of Pb was found in large snail size (1.1827 µg/g) at Station 2 and the highest Cr content was found in medium-sized snails (1.4053 µg/g) at Station 2. Highest Cu (27.9133 µg/g) in Station 1 and the highest Zn content was foundin large snail size (52.3273 µg/g) at Station 1. Average Cu and Zn content (23.7581 µg/g and 48.2146 µg/g) was higher than that of Pb and Cr (0.7800 µg/g and 1.0089 µg/g) in snails soft tissues. The MPI value of Singkep Island waters was 5.48 which was still relatively low. Based on the calculation of PTWI, gonggong snail in Singkep Island waters was still safe and feasible to be consumed for not exceeding the safe limits of consumption that has been determined, these are for Pb (8.9755 kg/week), Cr (6.3832 kg/week), Cu (41.2491 kg/week), and Zn (40.6516 kg / week). Key Words: Singkep Island, Heavy Metal, Pollution, Gonggong Snail (Strombus canarium)

DEGRADATION ABILITY AND IDENTIFICATION OF OIL DEGRADATION BACTERIA BY 16S rRNA SEQUENCING

Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 1 Januari s/d Juni 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTPollution in the marine environment caused by industrial activity, marine transportation, offshore mining and oil refineries, one of which comes from petroleum contamination. One of  efforts to prevent and to manage oil pollutionwas by the use of oil degrading bacteria. The objective of this research was to examine degradation ability of marine bacteria isolates and identify the bacterial isolate based on 16S rRNA sequence. The research was conducted from March to May 2018. The oil degradation analysis was conducted at Laboratory of Marine Microbiology,Faculty of Fishery and Marine Science, while the molecularidentification of bacteria was done in Genetics Laboratory, Faculty of Mathematics and Natural Sciences, University of Riau. The results showed that five isolates of oil degradation bacteria, selected(BM 1d, BM 7b, BM 7c, BM 14a and BM 14b) were able to degrade crude oil at different concentrations. The highest degradation at 1% concentration is indicated by BM 14b isolate, at 2% concentration was indicatedby BM 1d isolate and at 3% concentration was indicated BM 14b isolate. From the degradation analysis results, three isolates (BM 1d, BM 14a, BM 14b) have the highest degradation ability to determine the type of oil degrading bacteria. The molecular identification results by the 16S rRNA sequencing and BLAST analysis showed that BM 1d isolate were similar to Bacillus sp. (95%), BM 14a isolate were similar to Bacillus flexus (92%) and BM 14b isolate were similar to Bacillus cereus (98%). Keyword : Oil Degradation Bacteria , Crude Oil, 16S rRNA, Bacillus

ISOLATION OF OIL DEGRADATION BACTERIA FROM SEDIMENT IN SUNGAI PAKNING WATERS OF BENGKALIS REGENCY AND THE ABILITY TO DEGRADE CRUDE OIL

Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT Oil pollution in Bengkalis waters usually comes from land, marine transportation, defection port activities and oil industry. Oil pollution can be trapped in sediment because it can not be dissolved and difficult to degrade, one way to overcome pollution is by bioremediation. The purpose of this research was to isolate bacteria from sediment Sungai Pakning waters, to examine the ability of isolates in degrading crude oil and to identify the bacterial isolates genetically based on sequence 16S rRNA. The research was conducted from March to May 2018. Bacterial isolation was conducted in Laboratory of Marine Microbiology and the oil degradation analysis was conducted in Laboratory of Chemical Oceanography, Faculty of Fishery and Marine Science, University of Riau. This research used survey and experiment method, survey method to measuring of water quality, removal of sample and isolated of bacteria, experiment method to examine the ability of isolates in degrading crude oil. The result showed that eight isolates (7D15, 7D35, 7D23, 14D15, 14D34, 21D13, 21D23, 21D35) were able to degrade crude oil at 1-3% concentrations. The highest degradation at 1% concentration was indicated by 21D35 isolate, at 2%  concentration is indicated by  21D13 isolate, and at 3% concentration is indicated by 7D23 isolate. To molecular identification results by the 16S rRNA sequence take of three isolates have the highest degradation ability. 21D13 isolate was similar to Achromobacter pulmonis, 21D35 isolate was similar to Achromobacter sp., and 7D23 isolate was similar to Vibrio sp. Keyword : Pakning River, Oil Degradation Bacteria, 16 rRNA, Achromobacter, Vibrio

CHARACTERIZATION OF LACTIC ACID BACTERIA FROM CINCALUK BASEDON16S rRNA SEQUENCE

Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 1 Januari s/d Juni 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT Lactic acid bacteria (LAB) is a groupof bacteria frequently used as probiotic in aquaculture because of its beneficial effects on health, pathogenic bacteria prevention and increase of fish growth. LAB could be found in fermented fish products such as Cincaluk that is produced by people in Tembilahan and Bengkalis of Riau Province. The purpose of this research was to characterize LAB isolated from Cincaluk Tembilahan and Bengkalis genetically. This research was conducted from February until May 2018. Bacterial isolates were identified based on morphology, biochemical characters and 16S rRNA sequence. 24 isolates found were round, creamy white, Gram positive, did not produce indole, produced catalase, non motil, used citrate, did Methyl Red, produced H2S and gas, glucose, sucrose and lactose were fermented. Based on 16S rRNA sequence. Five of six isolates selected were identified as Lactobacillus plantarum (97-100% similarites) and one isolate was Bacillus cereus (94%) Keyword:Characteristic, Lactid Acid Bacteria, Cincaluk, Genetic,16S rRNA sequence