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Isolasi dan Karakterisasi Bakteri pada Ikan Kembung (Rastrelliger sp.) Asin Berkitosan

Biospecies Vol 6, No 01 (2013)
Publisher : Biospecies

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Abstract

Kitosan memiliki sifat antibakteri sehingga dapat dimanfaatkan sebagai bahan pengawet ikan. Penelitian ini dilakukan di Laboratorium Mikrobiologi Universitas Syiah Kuala, Banda Aceh dari bulan Mei sampai Agustus 2012, untuk mengisolasi dan mengkarakterisasi  bakteri pada ikan kembung (Rastrelliger sp.) asin berkitosan. Metode yang digunakan dalam penelitian ini deskriptif. Dari hasil isolasi didapat enam isolat yang memiliki morfologi koloni dan sel yang berbeda. Isolat yang memiliki bentuk bundar (50%), elevasi cembung (33,3%), tepian licin (50%) warna kuning (66,7%), sel bakteri gram positif (66,7%), bentuk sel bulat (100%), tidak berspora (66,7%) dan bersifat motil (66,7%).

BIOPROSPECTING SOIL ACTINOMYCETES: ISOLATION AND ANTIBACTERIAL ASSAY

Jurnal Biologi Edukasi Vol 4, No 1 (2012): Jurnal Biologi Edukasi
Publisher : Jurnal Biologi Edukasi

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Abstract

Searching for novel bioactive compounds of microorganisms is increasing. Actinomycetes are a group of bacteria that have potential as a major producer of bioactive compounds. This study aimed to isolate and evaluate the isolates of actinomycetes collected from soil. This research was carried out in three stages, namely the field sampling, isolation of actinomycetes, screening the potential as a producer of bioactive compounds (antimicrobials). A total of 11 isolates of actinomycetes have been isolated in this study. The isolates varied in morphological characteristics. The antibacterial evaluation showed that nine isolates (81.8%) that were able to inhibit either the test organism. Three isolates of ACT-04, ACT-05, ACT-06 and ACT-11 were capable of inhibiting both bacterial targets.

ISOLASI, KARAKTERISASI DAN UJI ANTIMIKROBA BAKTERI ENDOFIT ASAL TUMBUHAN JOHAR (Cassia siamea Lamk.)

Jurnal Biologi Edukasi Vol 4, No 1 (2012): Jurnal Biologi Edukasi
Publisher : Jurnal Biologi Edukasi

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Abstract

Endophytic bacteria are of alternative of antimicrobial compound producers. Their existence in the medicinal plants of johar (Cassia Siamea Lamk.) enables to produce bioactive compounds simmilar to those contained by the host plants. It is necessary to isolate and characterize endophytic bacteria from johar. Methods consisted of samples sterilization, endophytic bacterial isolation, and characterization of the morphology and physiology of Endophytic bacteria. a total of seven isolates of endophytic bacterial isolates were obtained from the isolation of plant Johar. Isolates DC 2, DC 3 and BC 3 had antibacterial capabilities with inhibitory zone diameters of 5 mm, 8.5 mm and 11.5 mm respectively.

Potensi Antibakteri Beberapa Tumbuhan Obat Tradisional

Jurnal Biologi Edukasi Vol 2, No 3 (2010): Jurnal Biologi Edukasi
Publisher : Jurnal Biologi Edukasi

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Abstract

The research of antibacterial ability from Anacardium occidentale (Anacardiaceae), Lawsonia inermis (Lythraceae), Averrhoa bilimbi, Averrhoa carambola (Oxalidaceae), Capsicum frutescens and Solanum torvum (Solanaceae) to Escherichia coli ATCC 25922 and Staphylococcus  aureus ATCC 29213 was done from Mei until November 2010 at Microbiology laboratory, Natural Science, Syiah Kuala University. Leave and barks extracts of plant was soluted using n-Hexane solution. Antibacterial activities was tested by using diffusion method with each concentrations 10%, 20%, and 30%. The result showed that plants leaves and barks had ability to inhibit the growth of Staphylococcus  aureus ATCC 29213. The largest of inhibition zone was formed by L. inermis leaves extract in 6 mm. Meanwhile C. frutescens barks extract was showed inhibition zone in 5 mm.

THE EFFECT OF STORAGE TIME ON TOTAL OF FUNGI IN KANJI PEDAH

Jurnal Natural Volume 11, Number 2, September 2011
Publisher : Syiah Kuala University

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Abstract

The research was conducted to determine the quality of the kanji pedah by counting total number and percentage of Aspergillus flavus and Aspergillus niger. It had been done since March from to October 2010 in Microbiology Laboratory of Biology Departement FMIPA Unsyiah. Kanji pedah as the sample was taken from Meunasah Mesjid Syamtalira village, Aron Subs-district of North Aceh. This research used sample from variety storage time groups range 3-7 days; 5-7 months; 9-12 months; and 15-18 months. Data were analyzed in descriptive and compared with the quality standard from Directorate General of National Agency of Drug and Food Control (Dirjen BPOM RI). The result showed the entire sample had fungi with ranged 1.45-63.7x102. The amount of fungi exceeds the quality standard already established by the National Agency of Drug and Food Control No. 05018/KBPOM/2001: 50 colony/g. Also Aspergillus flavus was found with range from 3%-27% meanwhile Aspergillus niger was found with range from 21,1%-40,9%.

INHIBITION TEST OF METHANOL EXTRACT FROM SOURSOP LEAF (Annona muricata Linn.) AGAINST Streptococcus mutans BACTERIA*

Jurnal Natural Volume 17, Number 1, March 2017
Publisher : Syiah Kuala University

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Abstract

Abstract. Dental caries is a disease with a high prevalence of caries in Indonesia is caused by the bacterium Streptococcus mutans. The leaves of the soursop (Annona muricata Linn.) is one of the herbs that can remove dental plaque. This study aims to determine the chemical content and measure the inhibitory antibacterial soursop leaft methanol extractin inhibiting the growth of Streptococcus mutans bacteria. Phytochemical screening stated that the leaf of the soursop contains flavonoids, alkaloids, tannins, steroids, glycosides and saponins. Test of inhibition were measured using paper disc diffusion method with variation concentrations of 5, 10, 15 20, and 25%, positive control is tetrasilkin 30 µg, and a negative control is methanol. Antibacterial test results showed that the methanol extract of soursop leaves have antibacterial activity against Streptococcus mutans at concentrations of 5, 10, 15, 20 and 25% with inhibitory diameter of 9,1; 10,57; 11,53; 12,01 and 13,75 mm respectively. Keywords: Soursop leaves, Dental caries, Streptococcus mutans, Methanol.*Judul ini telah dipresentasikan pada Seminar Nasional: Indonesian Students Conference on Science and Mathematics (ISCSM) 11-12 November 2015, Banda Aceh Indonesia

THE POTENT OF METHANOL EXTRACTS OF CASHEW (Anacardium occidentale L.) AGAINST METHICILLIN-RESISTANT Staphylococcus aureus (MRSA)

Jurnal Natural Volume 12, Number 2, September 2012
Publisher : Syiah Kuala University

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Abstract

Use of antibiotics including misuse and overuse has aided natural bacterial evolution by helping the microbes become resistant such as methicillin-resistant Staphylococcus aureus (MRSA). The MRSA resistant in disease treatment with commonly used antibiotics needs new drug to treat patients. Traditional herb can be alternative treatment such as cashew (Anacardium occidentale L.). Antibacterial activities of methanol extracts of stem cashew with concentration 10%, 20% and 30% showed zone of inhibition between 17 -20 mm.

IDENTIFIKASI BAKTERI ENDOFIT ASAL TANAMAN BELIMBING WULUH

Jurnal Biologi Edukasi Vol 5, No 1 (2013): Jurnal Biologi Edukasi
Publisher : Jurnal Biologi Edukasi

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Abstract

Isolat bakteri endofit DB2dan BUB2hasil isolasi dari tanaman belimbing wuluh memiliki kemampuan antijamur terhadap Candida albicans. Isolat DB2 memiliki kemampuan menghambat yang lebih luas dibandingkan dengan isolat BUB2. Hasil karakterisasi fisiologi menggunakan MICROBACTTM GNB 24E (OXOID) menunjukkan kedua isolat memiliki karakterisasi fisiologi yang berbeda. Hasil analisis gen 16S rRNA menunjukkan isolat DB2memiliki kemiripan sebesar 99% dengan Pseudomonas montelli.

Potency Study of N-Hexane Extracts of Black Plum (Syzygium cumini (L.) Skeels) in the Inhibitation Growth of Salmonella typhi and Candida sp.

Jurnal Natural Volume 14, Number 1, March 2014
Publisher : Syiah Kuala University

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Abstract

N-hexane extracts of the bark from black plum (Syzygium cumini (L.) Skeels) showed activity against Salmonella typhi. Meanwhile, the result showed that N-hexane was not able to inhibit the growth of  Candida sp. The research was done at Microbiology laboratory, Biology Department Faculty of Mathematics and Science,  Syiah Kuala University. The barks of black plum were collected from Aceh Besar District. The extraction method for black plum used maceration with N-hexane and than concentrated in rotary evaporator. The disc diffusion method used to screen the antimicrobial activity with 20 %, 30 %, 40 % dan 50 % concentration.

THE EFFECT OF METHANOL EXTRACT OF LEAVES OF HIBISCUS ROSA-SINENSIS L. AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA)

Jurnal Biologi Edukasi Vol 7, No 1 (2015): Jurnal Biologi Edukasi
Publisher : Jurnal Biologi Edukasi

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Abstract

This study was aimed to determine the effect of the methanol extract against bacteria Methicillin Resistant Staphylococcus aureus (MRSA) and to measure its inhibition capacity. Phytochemical test and extraction were conducted at the Research Laboratory of Chemistry Department, Faculty of Mathematics and Natural Sciences (MIPA). Antibacterial test was done at the Laboratory of Microbiology Department of Biological Science, University of  Syiah Kuala. The samples used leaves of H. rosa-sinensis collected from Gampong Sektor Timur, Darussalam.This study used completely randomized design (CRD) with five treatements and three replications namely; negative control using the solvent, extract test with  concentrations 0.2 g / mL, 0.3 g / mL, 0.4 g / mL, and  positive control using 30 µg linezolid. Phytochemical test showed that the fresh samples contain alkaloids, terpenoids, steroids, and flavonoids, while the methanol extract contains alkaloids and flavonoids. Antibacterial test showed that the methanol extract have inhibited MRSA bacterial growth, with the average of inhibition zone was 15.90 mm at the concentration of 0.4 g / mL.