N. Nurjanah
Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor Jalan Lingkar Akademik Kampus IPB Bogor 16680 Telepon (0251) 8622915 Faks.(0251) 8622916

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Effect of Feeding Diet Containing Different Dose of Vitamin E with 1:2 of n-3/n-6 Fatty Acid Content on Reproductive Performance of Female Zebrafish Danio rerio Utomo, N.B.P; Nurjanah, N.; Setiawati, Mia
Jurnal Akuakultur Indonesia Vol 5, No 1 (2006): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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Abstract

The role of essential fatty acids  as a precursor of prostaglandin and as a component of cell membrane in the form of phospholipids, can affect cell fluidity. Vitamin E functions as  fatty acid antioxidant in the body and affects egg quality.  Vitamin E and essential fatty acids (n-3 and n-6) are required at the same time in a proper  ratio to induce fish gonad maturation.  Zebra fish Danio rerio broodstock in 29 days pre-saline were fed on diet containing 4 different vitamin E concentration; i.e. 325, 375, 425 and 475 mg/kg diet with constant n-3 and n-6 fatty acid doses of 1% and 2%, respectively, for 6 weeks.  Reproduction performance in terms of fecundity was improved (p
Chemical Composition, Bioactive Components and Antioxidant Activities from Root, Bark and Leaf Lindur Dia, Siluh Putu Sri; Nurjanah, N.; Jacoeb, Agoes Mardiono
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 18, No 2 (2015): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

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Abstract

Plants lindur (Bruguiera gymnorrhiza) is one of the plants that have a potential asa source of bioactive compounds for the antioxidant. This study aimed to determine thechemical composition, bioactive compound and determine antioxidant activity of leaves,bark and roots of plants lindur (B. gymnorrhiza). This research was conducted throughseveral stages proximate test, stratified extraction, phytochemical test, and antioxidantactivity test with DPPH. The highest yield value resulting by ethanol extract of the leavesis 12,85 % and the lowest was produced by n-hexane root extract that is 0,18 %. The leavescontain high levels of protein, fat, and water which is the higher of the two parts from theother samples. Bark containing ash content (4,12%) and carbohydrates (46,02%). Root hasthe highest levels of carbohydrates that is 25,91 %. Bioactive components were detected onethanol and ethyl acetate extracts of the leaves are flavonoids, tannins, phenols, saponins,steroids and tritepenoid. Bark extract ethanol, ethyl acetate and ethanol root bark hasbioactive components there are flavonoids, tannins, phenols, saponins, and tritepenoid. Ethyl acetate extract of the roots only have bioactive components there are flavonoids,phenols, saponins, and triterpenoids. The potential antioxidant activity produced fromethyl acetate extracts of bark lindur with IC50 values is 14,21 ppm.Keywords: Antioxidants, phytochemicals, roots, ethanol, Bruguiera gymnorrhiza
Detection Tuna and Processed Products Based Protein and DNA Barcoding Wulansari, Nuring; Nurilmala, Mala; Nurjanah, N.
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 18, No 2 (2015): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

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Abstract

Tuna is the second largest fishery commodity in Indonesia after the shrimp. Since thehigh demand and the limited stock of tuna resulted in fraudulent chance. Authenticationis required to meassure consumers regarding the accuracy of its labeling and foodsafety. In this study, the authentication was based on protein and DNA barcoding usingcytochrome-b gene (cyt-b) of the mitochondrial DNA as the target of gene. Primer ofcyt b gene was designed based on the tuna species. This study aimed to identify theauthenticity of tuna fresh and its processed products through protein using SDS-PAGE and DNA barcoding techniques. The phases of this research were protein electrophoresisby SDS-PAGE, DNA extraction, PCR amplification, electrophoresis and sequencing.Samples of fresh fish (Tu1, Tu2, Tu3, Tu4, and Tu5) and processed tuna (canned andsteak) were successfully extracted. Result showed that SDS-PAGE proved the damage ofproteins in the processed tuna, so this method was not appropriate if it is used to identifythe authenticity of tuna. PCR electrophoresis results showed that the samples of tuna,tuna steak, sushi, meat ball, abon, and caned tuna were successfully amplified in the rangeof 500-750 bp except Ka3, which was in line with the target of DNA (620 bp). Resultedsequences of Tu2, Tu3, Tu4 and Tu5 were identified according the results of morphometricnamely T. albacares, while Tu1 was identified as T. obesus with homology level of 99%.Processed tunas (steak and canned tuna) were identified as T. albacares, as stated on thelabels.Keywords: Authentication, cytb, DNA barcoding, design primer, SDS-PAGE
PEMBELAJARAN DENGAN PENDEKATAN KONTEKSTUAL BERBANTUAN SOFTWARE FOCUSKY UNTUK MENINGKATKAN KEMAMPUAN BERPIKIR KRITIS DAN DISPOSISI MATEMATIS Najmul, I.; Cahya, E.; Nurjanah, N.
Jurnal Penelitian Pendidikan Vol 17, No 3 (2017): MANAJEMEN MUTU PENDIDIKAN DAN MODEL PEMBELAJARAN
Publisher : Universitas Pendidikan Indonesia

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Abstract

Penelitian ini bertujuan untuk mengkaji peningkatan kemampuan berpikir kritis dan disposisi matematis siswa SMA melalui pembelajaran dengan pendekatan kontekstual berbantuan software focusky. Desain penelitian yang digunakan adalah kuasi eksperimen. Populasi dalam penelitian ini adalah seluruh siswa kelas X di salah satu SMA di Kabupaten Karawang. Instrumen yang digunakan dalam penelitian ini adalah pretes dan postes untuk kemampuan berpikir kritis dan disposisi matematis. Hasil penelitian dianalisis dengan menggunakan Microsoft Excel 2013 dan SPSS versi 21. Hasil penelitian menunjukkan bahwa: 1) Peningkatan kemampuan berpikir kritis siswa yang memperoleh pembelajaran dengan pendekatan kontekstual berbantuan software focusky secara keseluruhan lebih baik daripada siswa yang memperoleh pembelajaran dengan pendekatan kontekstual 2) disposisi matematis siswa yang memperoleh pembelajaran dengan pendekatan kontekstual berbantuan software focusky tidak lebih baik daripada siswa yang memperoleh pembelajaran dengan pendekatan kontekstual.
Profile of Phenolic and Antioxidants Activity from Seaweed Extract Turbinaria conoides and Eucheuma cottonii Yanuarti, Rini; Nurjanah, N.; Anwar, Effionora; Hidayat, Taufik
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 20, No 2 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

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Abstract

Seaweed extract has ability to reduce some effects of free radicals because of phenolic compound. The purpose of this study was to determine total phenolic content, total flavonoids and antioxidant activity of seaweed extract from Turbinaria conoides and Eucheuma cottonii with different solvents. The extraction was carried out by n-hexane (non-polar) solvent, ethyl acetate (semi-polar) and methanol (polar). The results showed that the highest total phenol and flavonoids were found in  extract of Turbinaria conoides with ethyl acetate 211.00 mg GAE/g and 157.16 mg QE/g. Seaweed Eucheuma cottonii extract using methanol solvent had a higher phenol content 141.00 mg GAE/g, whereas the highest flavonoid content found in ethyl acetate solvent (35.1771 mg QE/g). The very strong antioxidant activity (< 50 μg/mL) was found in Turbinaria conoides and Eucheuma cottonii which extracted by methanol solvent (15.148 μg/mL and 23.154 μg/mL). Seaweed Turbinaria conoides and Eucheuma cottonii had a great potential as natural antioxidant.
Identification and Profiling of Active Compounds from Golden Apple Snail’s Egg Pigments Abdullah, Asadatun; Nurjanah, N.; Reyhan, Muhammad
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 20, No 2 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

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Abstract

Golden apple snail (Pomacea canaliculata) has been known as rice corps pest due to high adaptability and reproductive power. Utilization of Pomacea canaliculata’s eggs as raw materials in the food and health industry is one of the efforts to eradicate the pest snail. This study was aimed to identify the active compounds contained in the extract pigments of Pomacea canaliculata’s eggs. The methods of this study were extraction of pigments using acetone and methanol, analyzing the active compound (secondary metabolite) qualitatively, TLC to determine pigment components and LC-MS/MS to identify active compounds semi quantitatively. The results showed that active compounds in the methanol extract contain 11 carotenoid pigments of xanthophyl group, two carotenoid pigments of carotene group, and 2 active compounds in nonpigmented form, whereas the acetone extract contain 11 pigmentcarotenoids of xanthophyl group and 2 compounds active in non-pigment form.
Organoleptic Characteristics and Chemicals Ilabulo Catfish Fortification Harmain, Rita Marsuci; Dali, Faiza; Nurjanah, N.; Jacoeb, Agoes Mardiono
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 20, No 2 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

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Abstract

Diversification of traditional food ilabulo made from raw catfish (Pangasius sp.) has the potential to be developed in Gorontalo province to substitute chicken viscera. The research aimed to make ilabulo substitute the raw material of chicken viscera with the catfish fortified Kappaphycus alvarezii seaweed and catfish bone meal and to determine the organoleptic and chemical characteristics of ilabulo catfish fortification. Fortified treatment is K. alvarezii  and catfish bone (5 dan 10% ),  B (10 and 15%) and  C (15 dan 20%). The organoleptic analysis used a hedonic scale of favorite criteria on the appearance, texture, color, flavor, and taste. The results of organoleptic analysis continued with Bayes test. The chemical analysis used the Association of Official Analytical Chemist method. The result of the hedonic characteristic of ilabulo catfish fortification was on the appearance  neutral criteria – like (5.53–7.03), texture neutral criteria – rather like (5.8–7.1), aroma rather like (6.3–6.73), color neutral – like (6.1–7.03) and taste (6.07–6.53) neutral criteria – rather like. The result of Bayes test obtained by a texture of importance value 5, appearance importance value 5, aroma of importance value 4, color of interest value 3 and taste of importance value 2. Characteristic of ilabulo culture of selected fortified catfish that was the fortification of K. alvarezii seaweed 15% and catfish  bone flour (20%) (fortification C) obtained by water content 56.46%, ash 11.54%, protein 7.78%, fat 8.91%, coarse fiber 0.61%, carbohydrate 22.07% and calcium 0.315 %.
Antioxidant Activities of Various Brown Seaweeds from Seribu Islands Diachanty, Seftylia; Nurjanah, N.; Abdullah, Asadatun
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 20, No 2 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

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Abstract

Brown seaweeds contain various metabolites (carotenoids, laminarin, alginate, fucoidan, mannitol, and phlorotannin) as anticancer substances, antioxidants and chemopreventive agent against degenerative diseases. This research was aimed to determine the chemical properties and antioxidants activity of Sargassum polycystum, Padina minor and Turbinaria conoides. This research consisted of the proximate analysis, crude fiber, minerals, heavy metals, extraction, phytochemicals, analysis phenolic compound and antioxidant activity with DPPH, FRAP and CUPRAC methods. The results showed moisture content of S. polycystum, P.  minor, and T. conoides 24-31%, fat 0.23-0,47%, protein 3-4%, ash 27-45%, carbohydrate 26-38% and crude fiber 2-6%. Mineral Fe S. polycystum, P.minor and T.conoides about 0.03 g/kg to 0.39 g/kg, Ca 11.28 g/kg to 27.98 g/kg, K 13.86 g/kg to 41.15 g/kg, Na 16.31 g/kg to 20.51 g/kg and Mg 8.04 g/ kg to 18.68 g/kg. The range of heavy metals Pb S. polycystum, P.  m i n o r  and T. conoides 3.48 mg/kg to 12.68 mg/kg, Hg 0.24 mg/kg to 0.53 mg/kg and Cu 5.66 mg/kg to 7.02 mg/kg. Ethanol extract of S. polycystum, P. minor, and T. conoides contains alkaloids, phenolic, flavonoids, saponins, steroids, and triterpenoids. Total phenolic compounds of brown seaweeds ranged from 3758.97-8287.18 mg GAE/g. Brown seaweeds contain DPPH antioxidant activities within IC50 values of 1.9-9.6 mg/mL, FRAP 70.643-105.357 µmol Trolox/g and CUPRAC 85.268-201 µmol Trolox/g.