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MORPHOLOGYCAL AND MOLECULAR PARTIAL HISTONE-H3 CHARACTERIZATION OF BINTAN SEA SNAIL GONGGONG (STROMBUS SP.) AS A SPECIES VALIDATION Viruly, Lily; Andarwulan, Nuri; Suhartono, Maggy T.; Nurilmala, Mala
HAYATI Journal of Biosciences Vol. 26 No. 2 (2019): April 2019
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (658.468 KB) | DOI: 10.4308/hjb.26.2.%x

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Sea snail gonggong is an icon of Tanjungpinang-Riau Islands Province. It is a favorite seafood item in Riau Islands Province, and is high economic value but not known widely yet. Until now, sea snail gonggong has been highly exploited but the research on this snail is very limited. The aim of this study was to morphology and molecular characterization of Bintan gonggong snail (Strombus sp.) as a species validation. Bintan gonggong snail included thick-shelled gonggong and thin-shelled gonggong. Morphology identifcation of species Bintan gonggong snail was based on morphometric variability. Molecular identifcation used partial Histone-H3, MEGA version 6.06, and bioinformatics analysis. The result showed that the morphological identifcation of thick-shelled and thin-shelled gonggong based on shell width, the lip thickness, and total weight signifcantly di?erent, but other variables (i.e shell length, shell depth, aperture length, and gonggong weight) were not signifcantly di?erent (p<0.05). Resulted of a molecular identifcation with phylogenetic analysis that the thin-shelled and thick-shelled Bintan gonggong snails were 1 species and a genetic distance of 1%. They were not species Strombus canarium, Strombus vitatus, and Strombus epidromis. Bintan gonggong snails were Strombus turturella (Leavistrombus turturella). DNA sequences of Bintan gonggong have been registered in Gen-Bank with registration numbers MH348131 (thinshelled gonggong) and MH348132 (thick-shelled gonggong).
Perubahan kandungan vitamin dan mineral ikan kembung lelaki akibat proses penggorengan Nurilmala, Mala; ., Nurjanah; Febriyansyah, Reza; Hidayat, Taufik
DEPIK Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Vol 4, No 2 (2015): AUGUST 2015
Publisher : Faculty of Marine and Fisheries, Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.036 KB) | DOI: 10.13170/depik.4.2.2688

Abstract

Abstract. Indian mackerel (Rastrelliger kanagurta) is one of sea water fish in Indonesia. It is a good source of protein. This fish is consumed by vary processing methods. In addition, it is very known that frying is the favorite method for food processing including fish in Indonesia because of its specific taste. However there is no data found for changes of vitamin and mineral  compositions so far, thus our study investigated the effect of deep frying  using 4L palm oil at 180 oC for 5 minutes on vitamin A, B12, and minerals (Ca, Na,K, Fe, Zn, and Se) of this fish. The measurenment of vitamin A and B12 was carried  out by HPLC and  AAS for  mineral. The results showed that deep frying effected on vitamin A with significantly increased (P<0.05), on the other hand vitamin B12  decreased significantly (P<0.05).  Mineral Ca  increased significantly (P<0.05), however Na dan K  decreased significantly (P<0.05). No significant result for Fe and Zn. Furthermore, it is found that Se content was under limit detection.Keywords: deep frying; mineral; Rastrelliger kanagurta; vitaminAbstrak. Ikan kembung lelaki (Rastrelliger kanagurta) merupakan salah satu ikan laut Indonesia. Ikan ini  dikonsumsi  dengan berbagai metode pengolahan. Pengolahan dengan menggoreng merupakan metode yang sangat disukai di Indonesia karena akan menghasilkan rasa yang khas termasuk pada ikan kembung. Di sisi lain, belum adanya data yang ditemukan akibat metode penggorengan ini pada perubahan vitamin dan mineral ikan kembung, sehingga tujuan penelitian ini adalah menentukan pengaruh yang terjadi pada kandungan vitamin A, B12, dan mineral ikan kembung (Ca, Na, K, Fe, Zn, dan Se)  setelah digoreng dalam deep fryer menggunakan 4L minyak goreng pada suhu 180 oC selama 5 menit. Pengujian yang dilakukan yaitu, uji vitamin A dan B12 dengan HPLC, serta uji mineral dengan AAS. Hasil penelitian menunjukkan bahwa metode penggorengan deep fryingberpengaruh nyata (P<0,05) terhadap vitamin A, sedangkan vitamin B12menurun secara signifikan (P<0,05). Mineral Ca meningkat secara signifikan (P<0,05), sedangkan Na dan K  menurun secara signifikan (P<0,05) setelah proses penggorengan. Mineral Fe dan Zn tidak berubah secara signifikan. Selenium memiliki kandungan dibawah limit deteksi.Kata kunci: proses penggorengan; mineral; Rastrelliger kanagurta; vitamin; 
CHEMICAL, MICROBIOLOGY CHANGES AND DETECTION OF HDC GENE ON LONGTAIL TUNA THUNNUS TONGGOL DURING CHILLING TEMPERATURE STORAGE Nurilmala, Mala; Abdullah, Asadatun; Matutina, Vicentius Marco; Nurjanah; Yusfiandayani, Roza; Sondita, M. Fedi A.; Hizbullah, Hanifah Husein
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 11 No. 2 (2019): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (942.362 KB) | DOI: 10.29244/jitkt.v11i2.23007

Abstract

Histamine is a biogenic amine that appear during post moterm phase on the fish flesh that contain high content of histidine. The higher level of histamine can be reduced by good handling practice to maintain fish quality for example: using chilling temperature. This research aimed to determine chemical and microbiology changes from longtail tuna Thunnus tonggol and the time when hdc gene can be detected during chilling temperature storage 8±3°C. This research design was a completely randomized design (CRD) with parameters of differences in fish storage time (1,2,3,4,5,6,7 days) and ice ratio 1:1. The results showed that the tuna fish experienced quality deterioration for 7 days of storage. Organoleptic values and pH decreased during storage and on the seventh day the fish were in the rigormortis phase. TVB and TPC values increased during storage and on the sixth day storage has passed the safe limit for consumption. Histamine levels of this tuna on the seventh day were 1.96 ppm. HDc gene detection using the PCR method showed negative results in each treatment. The protein profile that was formed during storage displayed to separate because of the cathepsin activity.
PRODUCTION OF ALGINATE OLIGOSACCHARIDES (AOS) AS PREBIOTIC INGREDIENTS THROUGH BY ALGINATE LYASE ENZYME Afni, Fahriza Sri; Purwaningsih, Sri; Nurilmala, Mala; Peranginangin, Rosmawati
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 20 No. 1 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (331.108 KB) | DOI: 10.17844/jphpi.v20i1.16498

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Prebiotics is indigestible foods that can not be digested but can stimulate the growth and activity of bacteria in the digestive tract effecting human health. Alginate oligosaccharides (AOS) can be used as a source of prebiotic. That compounds can be produced enzymatically by cutting long chain alginates using alginate lyase. The aim of this study was to produce alginate lyase enzyme then producing Alginate oligosaccharides (AOS) as a prebiotic ingredients. The alginate lyase enzyme can be produced from Bacillus megaterium bacteria using a discontinuous fermentor. The enzyme was  optimum temperature of 45°C and an optimum pH of 7.0. Alginate oligosaccharides production was performed with the addition of different enzyme concentrations 25, 50, 75, and 100 U. The result of the addition of enzyme (25, 50,75 U) showed that the value of polymerization degrees (DP) were between 4-5. However, the addition of enzyme (100 U) was in the range of  DP 3-4. Bacterial probiotic growth test results of Bifidobacteria and Lactobacillus showed that 1% added AOS media were able to increase the growth of probiotic bacteria compared to themedia without addition of AOS. The addition Alginate lyase activity of 50 U in AOS production is the best treatment of both probiotic bacteria.
DNA MINI-BARCODES AS A MOLECULAR MARKER FOR VARIOUS HAIRTAIL FISH PRODUCTS TRACEABILITY Abdullah, Asadatun; Nurilmala, Mala; Sitaresmi, Kinanti Permata
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 22 No. 1 (2019): Jurnal Pengolahan Hasil Perikanan
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (492.61 KB) | DOI: 10.17844/jphpi.v22i1.25874

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Hairtail fishery is an important fishery commodity with high economic value as export commodity. Authentication based on DNA mini-barcodes can be applied to overcome seafood fraud and to support Indonesian traceability system. This study was aimed to design species-specific primers of DNA minibarcodes and authentication of various processed hairtail fish products such as smoked, baked, fried, canned, and boiled. Analytical method applied PCR-based of cytochrome c oxidase 1 (COI) full length andmini-barcodes. Mini-barcode primer specific for hairtail (Trichiurus lepturus) successfully amplified DNA of processed hairtail fish. The BLAST analysis showed the samples were identified as Trichiurus sp. and T. lepturus with homology level of 91% to 100%. Phylogenetic analysis showed the processed sample (LC dan LD) were in the same group with T. lepturus.
NUTRIENT COMPOSITION OF EEL ANGUILLA BICOLOR BICOLOR AND ANGUILLA MARMORATA Nafsiyah, Ikromatun; Nurilmala, Mala; Abdullah, Asadatun
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 21 No. 3 (2018): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (284.834 KB) | DOI: 10.17844/jphpi.v21i3.24733

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Eel (Anguilla sp.) is a highly nutritious Indonesian marine commodity. However, information on the eel nutrient content in Indonesia is still limited. This study was aimed to determine chemical properties of Indonesian eels,Anguilla bicolor bicolor and A. marmorata. Proximate, amino acid, fatty acid, and vitamin A analysis were carried out on the fresh eel. The A. bicolor bicolor  ontained 65.51% of moisture, 1.34% of ash, 16.78% of protein, 13.26% of fat, and 3.1% of carbohydrate. Meanwhile, results on A. marmorata showed it contained 57.17% of moisture, 1.09% of ash, 17.30% of protein, 21.35% of fat, and 3.12% of carbohydrate. Leucine and glutamic acid represented the highest essential and non-essential amino acid of both A. bicolor bicolor and A. marmorata, respectively. The highest saturated and monounsaturated fatty acid were palmitate and oleic acid for A. bicolor bicolor as well as A. marmorata. The content of vitamins in A. marmorata was higher than in A. bicolor bicolor.
HISTAMIN DAN IDENTIFIKASI BAKTERI PEMBENTUK HISTAMIN PADA TUNA MATA BESAR (THUNNUS OBESUS) Wodi, Stevy Imelda Murniati; Trilaksani, Wini; Nurilmala, Mala
Jurnal Teknologi Perikanan dan Kelautan Vol 9 No 2 (2018): NOVEMBER 2018
Publisher : Fakultas Perikanan dan Ilmu Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1973.836 KB) | DOI: 10.24319/jtpk.9.185-192

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Big eyes tuna (Thunnus obesus) is a species that potentially valuable as a source of protein and commercial aspect. In the perspective of quality and safety, inappropriate care during catching and processing leading to microorganism contamination and can cause health problems when the meat is consumed. Histamine content, total Histamine Forming Bacteria (HFB), and Total Plate Count (TPC) are the indicator in food safety and quality standard for tuna?s products. This research aims to measured histamine content, total HFB, TPC score and to identify the histamineforming bacteria in big eyes tuna observed during chilling temperature for nine days. There are three stages, sample preparation, storage, chemical and microbiology analysis. The During the observation period, histamine content, total HFB dan TPC score are increased in all part of the sample. Highest TPC sore is observed at day 9 which is 5,4 x 105 CFU/g in belly part, 5,1 x 105 CFU/g in dorsal part and 1,0 x 104 CFU/g in tail part respectively. For total HFB, 2,7 x 105 CFU/g in belly part, 1,4 x 105 CFU/g in dorsal part and 2,3 x 103 SFU/g in tail part respectively. For histamine content, all parts experienced increasing histamine measured 59,73 ppm, 131,10 ppm, and 96,04 ppm respectively. In this research, Bacillus subtilis is identified 99% as histamine forming bacteria in big eyes tuna.
EFFECTIVENESS OF ALKALI AND ACID TO PRODUCE COLLAGEN FROM FISH SKIN OF STRIPED CATIFISH Devi, Hilda Lu?lu?in Nanda Alfira; Suptijah, Pipih; Nurilmala, Mala
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 20 No. 2 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (361.823 KB) | DOI: 10.17844/jphpi.v20i2.17906

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Fish skin is one of the alternative sources contained high protein  to isolate collagen. Fish skin generally extracted by the method of acid, alkali and enzymes. The study aim to determine the effectiveness of NaOHand acetic acid on catfish (Pangasius sp.) skin extraction  process.  The concentrations of alkaline pretreatment were 0,05; 0,1; 0,15 and 0,2 M with the soaking time of 2, 4, 6, 8 and 10 h by NaOH replacement in every 2 h. The concentrations of acetic acid for hydrolisis process were 0.05; 0.1; 0.15 and 0.2 M with the soaking time of 1, 2, and 3 h. The experimental design used for pretreatment process is split splot, while for the hydrolysis process is factorial completely randomized design. The results showed that pretreatment with a concentration of 0.05 M NaOH for 4 h has a significant effect for eliminating non-collagen protein (p&lt;0.05). The acetic acid concentration of 0.15 M for 1 h also has a significant effect on fish skin swelling. The yield of striped catfish collagen was 17.272%, the protein content was 86%, and the viscosity was 12 cP. Fish skin extract was identified as type I collagen by functional groups and electrophoretic analysis. Collagen from striped catfish skin has ?1 and ?2 and protein structure with the molecular weight of ? chain were 94 and 98 kDa, meanwhile the molecular wheight of ? chain was 204 kD.
Improving DNA Barcode-based Fish Identification System on Imbalanced Data using SMOTE Kusuma, Wisnu Ananta; Noviana, Nurdevi; Hasibuan, Lailan Sahrina; Nurilmala, Mala
TELKOMNIKA (Telecommunication Computing Electronics and Control) Vol 15, No 3: September 2017
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (457.977 KB) | DOI: 10.12928/telkomnika.v15i3.5011

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Problem in imbalanced data is very common in classification or identification. The problem is raised when the number of instances of one class far exceeds the other. In the previous research, our DNA barcode-based Identification System of Tuna and Mackerel was developed in imbalanced dataset. The number of samples of Tuna and Mackerel were much more than those of other fish samples. Therefore, the accuracy of the classification model was probably still in bias. This research aimed at employing Synthetic Minority Oversampling Technique (SMOTE) to yield balanced dataset. We used k-mers frequencies from DNA barcode sequences as features and Support Vector Machine (SVM) as classification method. In this research we used trinucleotide (3-mers) and tetranucleotide (4-mers). The training dataset was taken from Barcode of Life Database (BOLD). For evaluating the model, we compared the accuracy of model using SMOTE and without SMOTE in order to classify DNA barcode sequences which is taken from Department of Aquatic Product Technology, Bogor Agricultural University. The results showed that the accuracy of the model in the species level using SMOTE was 7% and 13% higher than those of non-SMOTE for trinucleotide (3-mers) and tetranucleotide (4-mers), respectively. It is expected that the use of SMOTE, as one of data balancing technique, could increase the accuracy of DNA barcode based fish classification system, particularly in the species level which is difficult to be identified.
Molecular Identification of Microalgae BTM 11 and its Lectin Isolation, Characterization, and Inhibition Activity Mustopa, Apon Zaenal; Isworo, Rhestu; Nurilmala, Mala; Susilaningsih, Dwi
ANNALES BOGORIENSES Vol 20, No 2 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v20i2.251

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BTM 11 is unknown species of microalgae, but has active compounds that can inhibit viruses. One of proteins produced by microalgae is a lectin. Lectin is a carbohydrate-binding protein found in various microalgae that show antiviral and antibacterial activity. The purpose of this study was to perform identification of the species of microalgae BTM 11, isolation, characterization, and assay of lectin inhibitory activity. Microalgae BTM 11 was identified to have homology with Cyanobacterium 99% and Geitlerinema sp 98%. Lectin of microalgae BTM 11 was isolated by ammonium sulfate precipitation of 75% with a molecular weight of 17 kDa. Lectin protein activity of microalgae BTM 11 was able to inhibit the enzyme activity of RNA helicase hepatitis C by 57.90% and 27.55%. In addition, the protein was able to suppress the activity of Staphylococcus aureus ATCC 6538, E. coli EPEC K.1.1. and Salmonella typhii ATCC 25241. Activitiy of lectin was stable at 30 °C and was unaffected by the action of the enzyme. These results indicate that lectin of microalgae BTM 11 could be a alternative to antiviral and antibacterial proteins.