Articles

Found 11 Documents
Search
Journal : Alotrop

UJI AKTIVITAS ANTIOKSIDAN DAN ANTIBAKTERI EKSTRAK DAUN Ricinus communis L (JARAK KEPYAR)

Alotrop Vol 1, No 1 (2017)
Publisher : FKIP University of Bengkulu

Show Abstract | Original Source | Check in Google Scholar

Abstract

This study aims to determine the antioxidant activity of jarak kepyar’s  leaf extracts (Ricinus communis L.) and to determine of the fraction the growth of Erwinia carotovora. Jarak kepyar’s leaf extracted by maceration method using ethanol and fractionated using n-hexane and ethyl acetate. Antioxidant test using DPPH (1,1-diphenyl-2-picrylhydrazyl) with various concentrations of 125 ppm, 100 ppm, 75 ppm, 50 ppm, 25 ppm, 15 ppm and 5 ppm. Antibacterial test conducted on the active fractions as antioxidants using paper disc method and variations in the concentration of 1000 ppm, 500 ppm, 250 ppm, 125 ppm, 62.5 ppm. Phytochemical test results jarak kepyar’s leaf contain secondary metabolites are alkaloids, flavonoids, phenols and terpenoids. The test results demonstrate the antioxidant activity of ethyl acetate fraction is very strong as an antioxidant with IC50 values of 5.4 ppm and a strong ethanol fraction categorized as antioxidants IC50 of 99.8 ppm. Antibacterial test results showed inhibition of the leaf fraction of  jarak kepyar’s (Ricinus communis L.) against Erwinia carotovora categorized weak growth in ethanol fractions with inhibition zone diameter of 4 mm and being in ethyl acetate fraction with a diameter of 5 mm

AKTIVITAS ANTIOKSIDAN DAN ANTIBAKTERI EKSTRAK DAUN Jatropha gossypifolia L

Alotrop Vol 1, No 1 (2017)
Publisher : FKIP University of Bengkulu

Show Abstract | Original Source | Check in Google Scholar

Abstract

This study was conducted to determine the activity of Jatropha gossypifolia L (jarak merah’s) leaf extract as an antioxidant and antibactery against acne causing bacteria (Propionibacterium acnes). To obtain an J. gossypifolia L jarak merah’s leaf extract by maceration using 96% ethanol, and then fractionated with n-hexane and ethyl acetate. Testing of antioxidant activity by DPPH method and antibactery activity test by the paper disc method. Antioxidant activity test has done for ethanol fraction, ethyl acetate fraction and n-hexane fraction, while testing for antibacterial activity performed on fractions active as antioxidant. The test results demonstrate the antioxidant activity of ethyl acetate fraction and the ethanol fraction as a fraction very strong with IC50 value of 22.33 ppm for ethyl acetate fraction and 41.04 ppm for ethanol fraction. Then the fraction of ethyl acetate and ethanol fraction antibactery activity test, the result is known that both factions have the ability to inhibit the growth of bacteria Propionibacterium acnes demonstrated by the inhibition zone is formed. In 1000 ppm is formed diameter of the greatest inhibition zone that is equal to 6 mm for ethyl acetate fraction and 5 mm for ethanol fraction.

BIOSINTESIS NANOPARTIKEL PERAK MENGGUNAKAN EKSTRAK BUAH Passiflora flavicarva (MARKISA) UNTUK MENDETEKSI LOGAM BERAT

Alotrop Vol 1, No 1 (2017)
Publisher : FKIP University of Bengkulu

Show Abstract | Original Source | Check in Google Scholar

Abstract

The aim of this research was to synthesize silver nanoparticle (NPP) by using Passiflora flavicarva (Markisa) fruit extract as detector of heavy metal. The NPP particles produced were determined by their optimum condition on the ratio of the volume of  Passiflora flavicarva passion fruit extract to AgNO3 and the reaction time. The silver nanoparticles produced under optimum conditions will be tested for selectivity against several metals that are often present in the environment, once the selective metal is known then it can be sensitized to the metal using a UV-Vis spectrophotometer to determine the minimal concentrations that can be detected by the silver nanoparticles Formed. The results showed that Passiflora flavicarva passion fruit extracts could be used for the biosynthesis of  NPP particles because they contain vitamin C and flavonoids capable of reducing Ag+ to Ag0 in the form of NPP. The optimal conditions for forming NPP particles are in the 1: 2 ratio with a 2 hour reaction time. From the results of selectivity test of NPP to some heavy metals that often exist in environment, it is known that NPP selective to mercury metal. It was found that selective mercury was followed by a sensitivity test with a UV-Vis spectrophotometer, with sensitive NPP  up to a concentration of 6.7 ppm. From the results of this study it can be concluded that NPP particles formed can be used to detect mercury metal up to a minimum concentration of 6.7 ppm.

PEMANFAATAN ARANG AKTIF SABUT KELAPA SAWIT SEBAGAI ADSOBEN ZAT WARNA SINTETIS REACTIVE RED-120 DAN DIRECT GREEN -26

Alotrop Vol 1, No 1 (2017)
Publisher : FKIP University of Bengkulu

Show Abstract | Original Source | Check in Google Scholar

Abstract

The problem of environmental polution caused by waste of batik industry lately is increasing, so it needed a method to overcome that problem. The aim of this study was to determine the ability of activated charcoal from coconut fiber palm in adsorbing Reactive Red-120 and Direct Green-26 dyes in waste of batik along with determining the parameters of isotherms adsorption using UV-Vis Spectrophotometer analysis method. Variations of pH, contact time, adsorbent weight and temperature were carried out as variable to obtain optimum conditions of the adsorption process. The optimum of conditions for Reactive Red-120  occured at pH 3 and a contact time of 30 minutes, while Direct Green-26  occurred at pH 4 and a contact time of 40 minutes, with each adsorbent weight 150 mg and the temperature 30 °C. Adsorption isotherms determined by Freundlich and Langmuir models with maximum adsorption capacity (Qmax) were obtained for the Reactive Red-120  was 400 mg/g, while Direct Green-26 is 169 mg/g. 

UJI AKTIVITAS ANTIPLASMODIUM FRAKSI N-HEKSANA DAUN Peronema canescens TERHADAP Mus musculus

Alotrop Vol 1, No 1 (2017)
Publisher : FKIP University of Bengkulu

Show Abstract | Original Source | Check in Google Scholar

Abstract

Peronema canescens (Sungkai) has been used in Bengkulu as raw herbal remedy to reduce the fever, some people are using as a malaria drug. P.  canescens leaves contain alkaloids, flavonoids, tannins and terpenoids – steroids .This study aims to analyze the effect of P.  canescens leaves  n-hexane fraction.  against paracetemias in Mus musculus infected with Plasmodium berghei, then to prove whether the . P.  canescens leaves n-hexane fraction  has potential as alternative medicine for malaria .   To make P. canescens extract , leaves was macerated using EtOH (96%), then filtrate was evaporated using a rotary evaporator, then fractionated with n-hexane. The 25 healthy  M.musculus  weighing 20-40g each, infected with P.berghei, grouped into 5 ie group 1 (K-) was treated orally with aquades, group 2 (K +) was treated orally with 0.42 mg of Chloroquine, Group 3 (P1) Group 4 (P2), group 5 (P3) was treated orally with a P.  canescens leaves n-hexane fraction  each at a dose of 0.028 ,  0.056 and 0.084 g / kgBW. After 3 days of digestion for each treatment, the amount of erythrocytes was calculated under a microscope. : The giving of the with dose 0,028 g / kgBW able to inhibit paracetemia 33,49%,  0,056 g / kgBW able to  57.91% and  0,084 g / kgBW able to  61.69%   The conclusion is the P. canescens leaves n-hexane fraction  at a dose of 0.028 , 0.056 , and 0.084 g / kgBW orally wil be decreased the amount of paracetemia in M.musculus, and the higher the dose given the higher percent of the inhibition.

PENERAPAN MODEL PEMBELAJARAN PROBLEM BASED INSTRUCTION (PBI)DENGAN MENGGUNAKAN MEDIA AUDIO-VISUAL UNTUK MENINGKATKAN AKTIVITAS BELAJARDAN HASIL BELAJAR SISWA DI SMA N09 KOTA BENGKULU

Alotrop Vol 2, No 2 (2018)
Publisher : FKIP University of Bengkulu

Show Abstract | Original Source | Check in Google Scholar

Abstract

This research was aimed to find out the student’s improvement of  learning activity and learning outcome as the implementation of  Problem Based Instruction (PBI) by using audio-visual media. The subject of this research was the students at grade X MIPA 3 of SMA N 09 Bengkulu Ciyty academic year of 2017/2018 which consisted of 27 students, on the subject of of chemistry’s basic laws and stoichiometry. This research was conducted by the researcher by using three cycles which consisted of four steps; planning, action, observation and reflection. The data collections were tests and non tests which consisted of post test and student’s learning activity observation sheets. The data of this research was analyzed by using simple quantitative data analysis;   the student’s average score, the percentage of classical absorption and the percentage of classical learning mastery.   Based on the result of this research can be concluded that the implementation of   PBI by using audio-visual media improved student’s learning activity and learning outcomes in each cycle and achieved the clasical learning outcomes in cycle III. The improvements of student’s average score was; score 23 (Fair) of cycle I; score 26,33 (good) of cycle II; and score 28,66 (good) cycle III. The average score of teacher observation sheets of each cycles were; 25,66 fpr the first cycle (good), 28 for the second cycle (good), and 29,66 in the last cycle (good). There  was also  improvements of the students’ percentage of classical learning mastery from in cycle I 51,85%, to 66,66% in cycle II and 85,15% in cycle III.

PEMANFAATAN EKSTRAK AIR BUAH Flacourtia inermis Roxb. (LOBI-LOBI) SEBAGAI PENGAWET IKAN LAUT

Alotrop Vol 2, No 1 (2018)
Publisher : FKIP University of Bengkulu

Show Abstract | Original Source | Check in Google Scholar

Abstract

[THE  UTILIZATION OF   Flacourtia inermis Roxb. (LOB-LOBI ) FRUIT WATER EXTRACTS AS A MARINE FISH PRESERVATIVE] This research aims to determine the magnitude of the concentration and the length of time the optimum soaking water extracts of fruit Flacourtia inermis Roxb. (lobi-lobi ) as well as measuring its potential for use as a preservative in marine fish. Sample marine fish used is Kerapu Macan (Epinephelus fuscogultatus). The concentration of water extract from the fruit of F.inermis Roxb  is at levels at 10, 20, 30 and 40% with soaking time each at 1, 2 and 3 hours for any variation of the concentration. Observation of the level of the fish freshness is done with a variety of storage time of 12, 18, and 24 hours at room temperature to any variation of the concentration of extract and soaking time. The parameters used in determining the level of the freshness of the sample fish is a rate of Total Volatile Bases (TVB), moisture content, and pH. The results of the research done indicate that extracts water from the fruit of F.inermis Roxb L are potential for use as a preservative of marine fish based on the values of pH and the levels of TVB. The most optimum concentration and soaking time in Kerapu Macan fish preserve during its storage was soaking with a concentration of 30% for 1 hour, i.e., able to withstand the pace of the decline of the quality of the Kerapu Macan sample for 18 hours at room temperature: with the pH 6.7 and fish TVBvalue at 29.4 mgN/100 g.

POTENSI EKSTRAK METABOLIT SEKUNDER Eugenia uniflora L. SEBAGAI BAHAN PENGAWET TAHU

Alotrop Vol 2, No 1 (2018)
Publisher : FKIP University of Bengkulu

Show Abstract | Original Source | Check in Google Scholar

Abstract

[POTENTIAL OF Eugenia uniflora L SECONDARY METABOLITES EXTRACT AS A TOFU PRESERVATIVE]. The purpose of this research is to know the secondary metabolite compounds from the Eugenia uniflora L (Dewandaru) fruit extracts , as well as to measure the potential of water extract from the fruit of E. uniflora L for the use in the tofu preserve as well as measure the impact of the addition of water extract of the fruit of E. uniflora L against moisture, pH, protein on tofu. The process of preserving test against tofu on the research done by soaking time at 2, 3 and 4 hour with each variation of the concentration of extract at  10, 15 and 20% as well as the observation time at 24 and 48 hours for each treatment.The result of the test water extract of the fruit of the phytochemical profile E. uniflora L showed the content of alkaloids, flavonoids, phenolic , saponins and steroid compounds. Test results from the granting of water extract from the fruit of E.uniflora L in the preserve of tofu with the variation of concentration and soaking time is proven to affect the value of water content, pH, and protein on tofu that was tested.The results of the research done indicate that the granting of a water extract of the fruit of E.uniflora L proven useful to be used as a natural preservative for tofu at 24 hours with a 20% concentration and a soaking time at 2 and 3 hours. From the results, it can be concluded that water extracts from the fruit of E.uniflora L. potential to be used as a natural preservative for tofu.

UJI AKTIVITAS ASAP CAIR CANGKANG BUAH KARET (Hevea brassiliensis) DAN APLIKASINYA DALAM PENGHAMBATAN KETENGIKAN DAGING SAPI

Alotrop Vol 2, No 2 (2018)
Publisher : FKIP University of Bengkulu

Show Abstract | Original Source | Check in Google Scholar

Abstract

This research was aimed to know the degree of Fenol compound, total acid and the activity of liquid smoke of Rubber (Hevea brassiliensis) Seed Shell also the application in obstructing the rancidity of beef. The Fenol compound was determined by using Reagen Follon-Ciocalteu. The total acid was determined by using the method of titrated acids.The ability as the antioxidant was tested by DPPH method and the ability of obstructing the rancidity of beef was done by measuring the alteration of TBA value in beef as long as saved. The result was shown the fenol compound in liquid smoke of Rubber Seed Shell as much as 0,84 % and the acetic acid content was 4.725%. The antioxidant test was produced IC50 value as much as 101.27 ppm. According to the IC50 value which got from the test that the liquid smoke of Rubber Seed Shell were included in moderate category. The increment of the liquid smoke of Rubber Seed Shell were able to suppress the rancidity of beef during the storage time. It was marked by the decreasing of TBA value in beef as given in treatment A1 (liquid smoke 4% ) and A2 liquid smoke 6%. The treatment without the liquid smoke (A0) increased the TBA value from 0.05 mgMDA/Kg on day (0) to 0.615 mgMDA/Kg on day 6. The treatment of concentration liquid smoke 4% (A1)  increased  from  0.039  mgMDA/Kg  on  day  (0) to  0.395  mgMDA/Kg  on  day  6.  The  treatment  of concentration liquid smoke 6% (A2) increased from 0.031 mgMDA/Kg on day 0 to 0.209 mgMDA/Kg on day 6.

POTENSI SITOTOKSIK DAN ANTIBAKTERI EKSTRAK DAUN Laportea interrupta (L.) Chew (JELATANG AYAM) TERHADAP Staphylococcus aureus

Alotrop Vol 2, No 2 (2018)
Publisher : FKIP University of Bengkulu

Show Abstract | Original Source | Check in Google Scholar

Abstract

This research aims to determine the profile of phytochemicals, determine the level of cytotoxic extract toward the larva of Artemia salina Leach by using Brine Shrimp Lethality Test (BSLT) method, and to measure how much the activity of  antibacteria leaf ekstract of Laportea Interrupta (L.) Chew  (Jelatang Ayam )  toward the Staphylococcus aureus bacteria. The leaf of L. interrupta  (L.) Chew are dried and grinded well. The half of grinded sample are tested by phytochemical  profile and another one are ekstracted by using etanol liquid for three days then they are evaporated. The result of fitokimia leaf L. interrupta  (L.) Chew test contains secondary metabolite alkaloid,  tanin, terpenoid,  and saponin. The result of cytotoxic extract of leaf L. interrupta  (L.) Chew toward the A. salina Leach is obtained LC50 for 93,33 ppm, so, the L. interrupta  (L.) Chew is toxic because it is in the range of 31 ppm to 1000 ppm and it can be potentially as a anticancer agents. The eksract of Jelatang Ayam leaf can obstruct the growth of S. aureus, on 5 x 104 clear zone concentration that formed 9 mm being medium categorized. The more concentration of the Jelatang leaf ekstract then more its obstruction energy.