Yulita Nurchayati
Postgraduate Program, Faculty of Biology, Universitas Gadjah Mada, Indonesia

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Produksi Biomassa Tanaman Nilam (Pogostemon cablin) yang Ditanam pada Intensitas Cahaya yang Berbeda Darmanti, Sri; Nurchayati, Yulita; Hastuti, Endah Dwi; Syaifuddin, Mochammad
JURNAL ANATOMI FISIOLOGI Vol 17, No 1 (2009): Volume XVII, Nomor 1, Maret 2009
Publisher : JURNAL ANATOMI FISIOLOGI

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Abstract

Patchouli oil is found in all vegetative part of Pogostemon cablin. Therefore, a high biomass is important to increase production of patchouli oil. Biomass production is on of plant growth indicator. Plant growth is a effected by genetic and environmental factor. Light intensity will effect to chlorophyl syntesis, photosynthetic rate, transpiration and hormonal balance. The aim of this research is to study effect of light intensity on biomass production of Pogostemon cablin. Design of research is CRD (Complete Randomized Design), with one factor which is light intensity. There were difference of light intensity that use astreatments. These are : 96 LUX, 340 LUX and 780 LUX. Each treatment was replicated five times. Result indicated that, light intensity affect biomass production of Pogostemon cablin. At light intensity of 96 LUX, biomass productions is the most optimum. An increase of light intensity, biomass production ofPogostemon cablin is reduction.
Adaptasi Morfologi Fisiologi dan Anatomi Eceng Gondok (Eichhornia crassipes (Mart) Solm) di Berbagai Perairan Tercemar Haryanti, Sri; Hastuti, Rini Budi; Hastuti, Endah Dwi; Nurchayati, Yulita
JURNAL ANATOMI FISIOLOGI Vol 14, No 2 (2006): Vol. XIV, No. 2, Oktober 2006
Publisher : JURNAL ANATOMI FISIOLOGI

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The research was conducted to determine the morphology , physiology and anatomy adaptation from eceng gondok in waste water pollution. The research was done at the laboratory of Botany Structure and function. The design of the experiment use was split plot design based of Completely Randomized Design. First factor was density 2 and 4, second factor was 3 waste was LIK, drugs and metal foundry with water to control. The treatment was 3 replicates, The experiment was long 20 days with leaf morphology parameter ,growth/ physiology parameter ( long root, content of chlorophyl leaf) and anatomy parameter ( leaf,and stalk leaf and root). The result of the research indicated was leaf morphology adaptation on drugs waste and physiology adaptation on third waste and root anatomy adaptation was drugs waste with protrution branch root.
Kandungan Klorofil, Karotenoid, dan Vitamin C pada Beberapa Spesies Tumbuhan Akuatik Kurniawan, Madha; Izzati, Munifatul; Nurchayati, Yulita
JURNAL ANATOMI FISIOLOGI Vol 18, No 1 (2010): Vol. XVIII, No. 1, Maret 2010
Publisher : JURNAL ANATOMI FISIOLOGI

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Abstract

Aquatic plants have important role in water ecosystem. They serve as the main producer, oxygen suplier and heavy metal absorbtion. Beside that, aquatic plants also economically potencial, such as sources of chlorophyll, carotenoid and vitamin C. The aim of this experiment is to analize the content of total chlorophyll, carotenoids and vitamin C in 13 species of aquatic plants. It is expected that these plants maybe used for commercial purposes. The plants were collected from Rawa Pening, Genuk stream, and brackish water shrim pond in Kendal and Jepara. These chemical analysis were done by spectrophotometer, whereas vitamin C content was measured using iodometric titration method. Results indicated that the highest content of chlorophyl was resulted by Ipomoea aquatica, which is 22,1 mg/L. The highest content of carotenodid and vitamin C is resulted by Nymphaea sp., which were 3,42 mg/L and 14,1 mg/30 g respectively. It is concluded that Ipomoea aquatica and Nymphaea sp. have good commercial value as sources of pigment and vitamin C.
Respon Pertumbuhan dan Produksi Alkaloid pada Kalus Berakar Datura metel L. terhadap Peningkatan Mikronutrien dari Medium MS Hadayani, Wahyu; Nurchayati, Yulita; Setiari, Nintya
JURNAL ANATOMI FISIOLOGI Vol 12, No 1 (2012): VOL XX, NOMOR 1, TAHUN 2012
Publisher : JURNAL ANATOMI FISIOLOGI

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Senyawa alkaloid dari tanaman Solanaceae memiliki potensi obat antara lain sebagai antibakteri bahkan memberi efek halusinasi. Alkaloid pada kecubung, Datura metel disintesis pada organ akar dan diakumulasi pada bagian pucuk. Salah satu metoda untuk produksi senyawa alkaloid tersebut adalah dengan menginduksi perakaran dari kalus yang berasal dari daun (induksi kalus berakar). Produksi alkaloid dapat ditingkatkan dengan memodifikasi komponen mikronutrien dari medium dasar MS (Murashige&Skoog).  Penelitan ini bertujuan untuk mempelajari pengaruh peningkatan konsentrasi mikronutrien terhadap pertumbuhan dan kandungan alkaloid total dari kalus berakar.  Kultur diperoleh dari induksi kalus dalam medium MS tanpa zat tumbuh dengan perlakuan konsentrasi mikronutrien.  Pertumbuhan kultur ditentukan dari berat segar dan berat kering,   sedangkan kandungan alkaloid total dianalisis dengan metoda titrasi. Data dianalisis secara deskriptif. Hasil penelitian menunjukkan  bahwa peningkatan konsentrasi mikronutrien hingga 2,5 kali dari medium MS menghambat pertumbuhan kalus berakar. Namun demikian, kondisi ini tampak tidak mampu memacu produksi senyawa alkaloid dari kultur.
Pertumbuhan Tongkol Jagung Baby Corn (Zea Mays L.) Varietas Pioneer-11 Setelah Pemberian Kascing Nurchayati, Yulita; Yuliana, Titis
JURNAL SAINS DAN MATEMATIKA Volume 14 issue 4 Year 2006
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

ABSTRACT---An experiment to obtain high productivity of baby corn’s cob by casting treatment has been conducted. The aims of this experiment were to study the casting effect for baby corn productivity and to obtain the efficient dose of casting to increase baby corn productivity. This experiment was done by a Randomized Complete Design with four treatment and seven replication. The treatments were four kinds of casting dose, i.e 0g/plant, 200g/plant, 400g/plant and 600g/plant in their growth media. The measurable parameter were fresh weights, dry weights and cob quantity of baby corn. The casting was analyzed qualitatively including C/N ratio and organic material of casting. The result showed that the casting can increase the cob of baby corn productivity eventhough the efficient doses of casting to increase the baby corn productivity were not obtained.   Keywords: baby corn, casting, cob
Respon Pertumbuhan dan Produksi Senyawa Antioksidan pada Kalus Hibiscus sabdariffa L. dari Eksplan yang Berbeda secara in vitro Noviati, Agustin; Nurchayati, Yulita; Setiari, Nintya
JURNAL SAINS DAN MATEMATIKA Volume 22 Issue 1 Year 2014
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

Ascorbic acid and carotenoid are secondary metabolites found in roselle, which show antioxidant activity. These compounds can be obtained from callus induced  by several kinds of explants. The aims of this experiment is to study callus growth from explants which can encourage high level of antioxidant compounds. The callus was obtained from difference organ, i.e. section of leaf, petiole and flower sepal. Sterilized explants were planted in MS (Murashige&Skoog) combined with 2 mg/L Naphtalene Acetic Acid (NAA) dan 5 mg/L Benzyl Adenin (BA).  This experiment has been conducted by completly randomized design with 5 replicates. Besides fresh weight callus, callus respons from each explants were analyzed descrptively.  Ascorbic acid and carotenoid content were analyzed qualitatively and quantitatively by titration and spectrophotometric respectively.  The results showed that all kinds of explant dedifferentiated into callus which antioxidant content. Callus from leaf section had the higgest fresh weight with high level ascorbic acid. Whereas the higgest carotenoid level was obtained from callus-derived flower sepals. It conclused that  in vitro callus was useful for producing plant biochemical compounds. Keywords : Callus induction, antioxidant agents, explants, ascorbic acid, cartenoid
Respon Pertumbuhan dan Produksi Senyawa Antioksidan pada Kalus Hibiscus sabdariffa L. dari Eksplan yang Berbeda secara in vitro Noviati, Agustin; Nurchayati, Yulita; Setiari, Nintya
SAINS DAN MATEMATIKA Volume 22 Nomor 1 Tahun 2014
Publisher : SAINS DAN MATEMATIKA

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Abstract

Ascorbic acid and carotenoid are secondary metabolites found in roselle, which show antioxidant activity. These compounds can be obtained from callus induced  by several kinds of explants. The aims of this experiment is to study callus growth from explants which can encourage high level of antioxidant compounds. The callus was obtained from difference organ, i.e. section of leaf, petiole and flower sepal. Sterilized explants were planted in MS (Murashige&Skoog) combined with 2 mg/L Naphtalene Acetic Acid (NAA) dan 5 mg/L Benzyl Adenin (BA).  This experiment has been conducted by completly randomized design with 5 replicates. Besides fresh weight callus, callus respons from each explants were analyzed descrptively.  Ascorbic acid and carotenoid content were analyzed qualitatively and quantitatively by titration and spectrophotometric respectively.  The results showed that all kinds of explant dedifferentiated into callus which antioxidant content. Callus from leaf section had the higgest fresh weight with high level ascorbic acid. Whereas the higgest carotenoid level was obtained from callus-derived flower sepals. It conclused that  in vitro callus was useful for producing plant biochemical compounds. Keywords : Callus induction, antioxidant agents, explants, ascorbic acid, cartenoid
KANDUNGAN ASAM ASKORBAT PADA KULTUR KALUS ROSELA (hibiscus sabdariffa L.) DENGAN VARIASI KONSENTRASI SUKROSA DALAM MEDIA MS Nurchayati, Yulita; Afiah R, Fathiyah
Majalah Obat Tradisional Vol 15, No 2 (2010)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/mot-TradMedJ15iss2pp%p

Abstract

Asam askorbat merupakan salah satu antioksidan yang dijumpai dalam kelopak bunga rosela. Produksinya secara konvensional memerlukan waktu dn lahan yang luas, sehingga diperlukan metode alternatif menggunakan pembentukan kalus secara in vitro.  Sukrosa  berperan sebagai sumber karbon utama dalam medium MS, slain itu dapat berperan sebagai prazat pembentukan asam askorbat.  Penelitian ini bertujuan untuk mengkaji pengaruh penambahan sukrosa dalam pembentukan kalus dan produksi asam askorbat.  Perlakuan yang diberikan adalah beberapa konsentrasi sukrosa yaitu 20 g/L, 30 g/L, 40 g/L dan 50 g/L dalam medium kultur.  Kultur diinkubasi selama 42 hari dengan subkultur 2 kali setiap 10 hari sekali. Analisis kandungan asam askorbat dilakukan dengan metode titrasi iodometri. Hasil penelitian menunjukkan bahwa kalus terbentuk paling cepat pada penambahan sukrosa 20 g/L sedangkan kandungan asam askorbat tertinggi diperoleh pada kalus dengan penambahan sukrosa 50 g/ L.
Respon Pertumbuhan dan Produksi Senyawa Antioksidan pada Kalus Rosela (Hibiscus sabdariffa L.) dari Eksplan yang Berbeda secara in vitro Noviati, Agustin; Nurchayati, Yulita; Setiari, Nintya
Bioma Vol. 14, No. 2, Tahun 2012
Publisher : Bioma

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (443.682 KB) | DOI: 10.14710/bioma, 14, 2, 85-90

Abstract

Callus induction method can be used as tool in producing plant secondary metabolites. One of this compound found in roselle (Hibiscus sabdariffa L.) is antioxidant agent i.e ascorbic acid and carotenoid. The callus could be induced from any kind of explants in tissue culture. The aims of this experiment is to select explant which can encourage of callus formation beside high level of antioxidant compounds. The treatment of experiment was three kinds of explants i.e section of leaf, petiole and flower sepal. Sterilized explants were planted in MS (Murashige&Skoog) combined with 2 mg/L NAA dan 5 mg/L BAP.  This experiment has been conducted by completly randomized design with 5 replicates Growth variable, included fresh weight callus, growth presentation, callus respons and biochemical variable like content of ascorbic acid and carotenoid were analyzed.  The data were analyzed by analysis of varian and Duncan’s test at 95% significant level. Thed results showed that all kinds of explant dedifferentiated into callus which antioxidant content. Callus from leaf section had the higgest fresh weight with high level ascorbic acid. Whereas the higgest carotenoid level was obtained from callus-derived flower sepals. It conclused that  in vitro callus was useful for producing plant biochemical compounds.   Keywords : Callus induction, antioxidant agents, explants, ascorbic acid, cartenoid
PENGARUH ALELOKIMIA EKSTRAK TANAMAN PADI (Oryza sativa L. Var. IR64) TERHADAP PERKECAMBAHAN DAN PERKEMBANGAN KECAMBAH KEDELAI (Glycine max L.) Mahayaning, Fitrian Agna; Darmanti, Sri; Nurchayati, Yulita
JURNAL ANATOMI FISIOLOGI Vol 23, No 2 (2015): Volume XXIII, Nomor 2, Oktober 2015
Publisher : JURNAL ANATOMI FISIOLOGI

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Abstract

The aim of this research is to know the allelochemicals effect of  rice var. IR 64 residues extract on germination and growth soybean var. Grobogan seedling. This experiment  uses completely randomized factorial design (3x5). The fist factors is extract resources (root, straw, and hull) and secont factor is level concentration extrac (0%, 25%, 50%, 75%, dan 100%) with 5 replicates. Data analysed using ANOVA and DMRT.The results showed that allelochemicals from residue of rice IR 64 extract inhibit the germination and growth soybean var. Grobogan seedling, the higher concentration of the treatment the greater inhibition. Inhibition most likely caused by a hull rice extract.   Keywords: Glycine max, Oryza sativa, allelochemical, phenol