Wisnu Nurcahyo
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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Journal : Jurnal Sain Veteriner

STUDI RESPON IMUN PADA AYAM YANG DIIMUNISASI DENGAN ANTIGEN EKSKRESI SEKRESI SPOROZOIT Eimera tenellla

Jurnal Sain Veteriner Vol 22, No 2 (2004)
Publisher : Fakultas Kedokteran Hewan

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STUDI EKSPERIMENTAL SISTA JARINGAN Toxoplasma gondii SECARA IN VIVO AN EXPERIMENTAL STUDY OF Toxoplasma gondii TISSUE CYST IN VIVO

Jurnal Sain Veteriner Vol 21, No 2 (2003)
Publisher : Fakultas Kedokteran Hewan

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Pengaruh Infeksi Toxoplasma gondii Isolat Lokal terhadap Gambaran Darah pada MEncit (Mus musculus)

Jurnal Sain Veteriner Vol 22, No 1 (2004)
Publisher : Fakultas Kedokteran Hewan

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THE EFFECT OF SEVERAL DESINFECTANTS ON THE SPORULATION OF THE ERIMERIA TENELLA OOCYST

Jurnal Sain Veteriner Vol 17, No 1 (1999)
Publisher : Fakultas Kedokteran Hewan

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A research has been conducted to evaluate the effect of certain desinfectants on the speculation of the Eimerla tenclla oocysts.In this research in vitro methode has been used. This research used oocysts Eimeria tenella which have not yet Sporulated. 6 kinds of disinfectants and water as control. These desinfectants were used in the research namely : Lysol, Creolin. Kalium Jodida (KJ). Formalin, Bromoquod and Ca (OH)2.The oocysts which have not yet sporulation were imerted in these desinfectants. The duration of liners ion at each treatment was succescively 10 minutes. 20 minutes and 30 minutes. The desinfectants concentration used is of three degree of strength, in the following proportion = 1:2:4.The data colected was the percentage of oocystys which have sporulated 24 hours, 48 hours and 72 hours, after treatment. The data was analyzed with the Split Plot method (Gill. 1978).The result of research showed that each desinfectant had a different effect on the percentage of the oocyst sporulation (P < 0,01) . Bromoquod was found to be the best desinfectant, and the others were in this following order = Creolin. KJ, Water, Lysol. CafQHJn and formalin. The Concen­tration was also found significantly effective (P < 0,6) in suppressing the sporulation of the oocysts. Creolin concen­tration of the 4tri strength was found to be the best desin­fectant and the others were in this following order : water. KJ. Bromoquod, Lysol, Ca(OH)£ and formalin. The duration of imersion had no significant influence

Monitoring of Physiological and Parasites Status of Bawean Deer (Axis Kuhlii) in Its Habitat as a Baseline for Wildlife Conservation Endeavor

Jurnal Sain Veteriner Vol 33, No 2 (2015): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

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AbstractThe research on physiological and reproduction status of Bawean deer (Axis kuhlii) in its habitat has been conducted, to understand and to find out as a basic information on Bawean deer (Axis kuhlii) in its habitats a baseline data for wildlife conservation efforts. The deer is categorized as an endangered animal, therefore, more attention was given toward Bawean deer conservation. Habitat changes, loss of habitat, fragmentation and illegal hunting might caused the wild animals become more marginalized and the populations have been declined.Therefore, it is needed to have research as an effort to save Bawean deer in its natural habitat. This research activity covered monitoring on physiological and reproduction status, and also the examination of deers feces samples was parasite coprological data that serve as a basic consideration data in conservation and determination of Bawean deers health in the nature and/or conservation. The research was done in Bawean Island, Gresik and East Java, by taking faces of Bawean deers in the nature and conservation. Data gathering of physiological andreproduction status were done by examining deers condition, either directly in conservation or in its habitat, and also by interviewing people around the forest. The data on physiological status, behavior monitoring, reproduction status, faces samples, blood, food analysis, and interview result were analyzed descriptively. Some worm eggs were found during feces examination, those were Strongyl, Strongyloides sp, Trichuris sp, Fasciola gigantica and Oocysta coccidia. The result showed that the same parasites were found in the cattle and goat raised by people around in the vicinity of the forest, meaning, there was an interaction between wildlife andlivestock. According to the result, it is needed to monitor the physiological status of Bawean deers routinely since Bawean deers is classified as endangered species.

STUDI EKSPERIMENTAL SISTA JARINGAN Toxoplasma gondii SECARA IN VIVO AN EXPERIMENTAL STUDY OF Toxoplasma gondii TISSUE CYST IN VIVO

Jurnal Sain Veteriner Vol 21, No 2 (2003): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

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Daya Ovicidal Ekstrak Kulit Buah Muda (Calotropis procera) terhadap Haemonchus contortus secara in vitro

Jurnal Sain Veteriner Vol 33, No 2 (2015): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

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Penelitian ini bertujuan untuk mengetahui konsentrasi efektif ekstrak kulit buah muda Calotropis procera (C. procera) dalam menghambat perkembangan telur cacing Haemonchus contortus secara in-vitro. dari 0,2g/ml sediaan larutan ekstrak stok, albendazole konsentrasi 0,055%. Data hasil penelitian dianalisis secara deskriptif. Konsentrasi efektif ekstrak kulit buah muda C. procera (EKBMCP) dalam menghambat perkembangan daya tetas telur cacing Haemonchus contortus secarain-vitro adalah perlakuan EKBMCP konsentrasi 4,5% dengan daya hambat 88% sedangkan  perlakuan EKBMCP konsentrasi 2,5% dan 3,5% daya hambatnya secara berturut-turut adalah 70,5% dan 81%.

Monitoring of Physiological and Parasites Status of Bawean Deer (Axis Kuhlii) in Its Habitat as a Baseline for Wildlife Conservation Endeavor

Jurnal Sain Veteriner Vol 33, No 2 (2015): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

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Abstract

The research on physiological and reproduction status of Bawean deer (Axis kuhlii) in its habitat has been conducted, to understand and to find out as a basic information on Bawean deer (Axis kuhlii) in its habitats abaseline data for wildlife conservation efforts. The deer is categorized as an endangered animal, therefore, more attention was given toward Bawean deer conservation. Habitat changes, loss of habitat, fragmentation and illegalhunting might caused the wild animals become more marginalized and the populations have been declined. Therefore, it is needed to have research as an effort to save Bawean deer in its natural habitat. This research activity covered monitoring on physiological and reproduction status, and also the examination of deers feces samples was parasite coprological data that serve as a basic consideration data in conservation and determination of Bawean deers health in the nature and/or conservation. The research was done in Bawean Island, Gresik and East Java, by taking faces of Bawean deers in the nature and conservation. Data gathering of physiological and reproduction status were done by examining deers condition, either directly in conservation or in its habitat, and also by interviewing people around the forest. The data on physiological status, behavior monitoring, reproduction status, faces samples, blood, food analysis, and interview result were analyzed descriptively. Some worm eggs were found during feces examination, those were Strongyl, Strongyloides sp, Trichuris sp, Fasciola gigantica and Oocysta coccidia. The result showed that the same parasites were found in the cattle and goat raised by people around in the vicinity of the forest, meaning, there was an interaction between wildlife andlivestock. According to the result, it is needed to monitor the physiological status of Bawean deers routinely since Bawean deers is classified as endangered species.

Daya Vermisidal Ekstrak Lima Jenis Etnofarmakologi terhadap Cacing Haemonchus contortus Secara In-vitro

Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

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       Research aimed to identify the most effective concentration of five extract types (EKBMCP, EKPAS, EDMSP, EBJCP, dan EDMCO) as vermicidal anthelmintic against          H. contortus in-vitro. Research was consisted of seven groups, among them five groups were considered as treatment groups that named based on the extract type (EKBMCP, EKPAS, EDMSP, EBJCP, dan EDMCO). Each treatment group was consisted five subgroups of etnopharmacological concentration : 0,5%, 1,5%, 2,5%, 3,5% dari 0,2 g/mL. Other two group were regarded as a negative control by distilled water treatment, and a positive control by pyrantel pamoat 5%. Every single treatment group was quadruplet and exposure each concentration were performed in quadruplet with variation in exposure times i.e. : 1, 3,             5, and 7 hours. The research focused on several variables that were the percentage of live and dead H. contortus in the treatment groups, extract concentration, and treatment period by using selected etnopharmacological extract. The effective vermicidal anthelmentic concentration of each treatment was analyzed descriptively. The results showed that five etnopharmacological extract types that were EKBMCP, EKPAS, EDMSP, EBJCP, dan EDMCO had a potency as vermicidal anthelmentic. Among those treatment, EKPAS and EBJCP had the lowest concentration (2,5%) to produce optimum vermicidal effect (100%) with period five hours exposure. Other treatment groups with EKBMCP and EDMSP needed 3,5% concentration with exposure period seven hours to produce optimum vermicidal effect, respectively. Another treatment group with EDMCO needed 3,5% concentration with exposure time seven hours to produce optimum vermicidal effect. 

DAYA OVISIDAL DAN LARVISIDAL INFUSA DAUN BAMBU APUS (Gigantochloa apus)TERHADAP HAEMONCHUS CONTORTUS SECARA IN VITRO

Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

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Gastrointestinal Nematodes are one among cause decrease production efificiency in goat and sheep in Indonesia, especially parasite of abomasum namely Haemonchus contortus. This parasite causes disease of Haemonchosis causing anemia, and causes mortality to young goat or sheep. Loss of economics caused by high morbidity and  mortality this parasite so that can improve the expense of prevention and medication of this disease. This research aim was to evaluate ovicidal and larvicidal apus bamboo leaf infusion (Gigantochloa apus) against Haemonchus contortus by in vitro. Examination of apus bamboo leaves infusion to seven concentrations namely : 1.2; 1.0; 0.8,; 0.6; 0.4; 0.2;0.1; 0.05 and 0 mg / ml as negative control. Positive control uses Albendazole with concentration 0,025 mg / ml. The highest concentration 1.2 mg / ml apus bamboo leaves infusion   inhibited 92.35% egg hatching ( LC 0.49 mg / ml ) and have larvicidal activity equal to 90% ( LC50 0,47mg / ml). The concentration 1.2 mg / ml also have similar effect with positive control which use Albendazole. Phytochemical apus  bamboo leaf analysis of the highest concentration revealed the presence  tanin condensed, saponins, and alkaloids.